| escherichia albertii sp. nov., a diarrhoeagenic species isolated from stool specimens of bangladeshi children. | the taxonomic position of a group of five d-sorbitol- and lactose-negative enterobacterial isolates recovered from diarrhoeal stools of children at the international centre for diarrhoeal disease research, bangladesh (icddr,b), was investigated by dna-dna hybridization, phenotypic characterization and 16s rdna sequencing. these strains were originally identified as 'hafnia alvei-like' with the api 20e system but, in fact, show more phenotypic and genotypic resemblance to members of the genus esc ... | 2003 | 12807204 |
| biochemical properties of a newly described escherichia species, escherichia albertii. | five strains of a newly described escherichia species, escherichia albertii, were extensively characterized by conventional biochemical methods and by commercial identification panels. e. albertii is an indole-negative species that ferments d-mannitol but not d-xylose. because these strains are not included in the databases of commercial systems at present, they were most often identified as hafnia, salmonella, escherichia coli, or, on one system (microscan dried overnight panels), yersinia ruck ... | 2003 | 14532241 |
| evolutionary genetics of a new pathogenic escherichia species: escherichia albertii and related shigella boydii strains. | a bacterium originally described as hafnia alvei induces diarrhea in rabbits and causes epithelial damage similar to the attachment and effacement associated with enteropathogenic escherichia coli. subsequent studies identified similar h. alvei-like strains that are positive for an intimin gene (eae) probe and, based on dna relatedness, are classified as a distinct escherichia species, escherichia albertii. we determined sequences for multiple housekeeping genes in five e. albertii strains and c ... | 2005 | 15629933 |
| natural antimicrobial susceptibility patterns and biochemical identification of escherichia albertii and hafnia alvei strains. | bangladeshi diarrheagenic hafnia alvei-like strains have been described recently as the new species escherichia albertii (int j syst evolut microbiol. 2003;53:807-810). the natural susceptibility of 21 e. albertii and 76 h. alvei strains to 69 antimicrobial agents was examined, applying a microdilution procedure in isosensitest broth (for all the strains) and cation-adjusted mueller-hinton broth (for some strains). examining the phenotypic features of both taxa with commercial identification sys ... | 2005 | 15766600 |
| the genus hafnia: from soup to nuts. | the genus hafnia, a member of the family enterobacteriaceae, consists of gram-negative bacteria that are occasionally implicated in both intestinal and extraintestinal infections in humans. despite the fact that the genus currently contains only a single species (h. alvei), more extensive phylogenetic depth (two or more species) is apparent based upon dna relatedness and 16s rrna gene sequencing studies. hafnia causes a variety of systemic infections, including septicemia and pneumonia; however, ... | 2006 | 16418520 |
| allelic subtyping of the intimin locus (eae) of pathogenic escherichia coli by fluorescent rflp. | intimin is a highly polymorphic protein encoded by the eae gene and plays a crucial role in the attaching-effacing phenotype of diarrheagenic escherichia coli and related pathogens. we have developed a method to quickly and accurately uncover allelic variation at the eae locus through the use of fluorescent rflp (frflp). application of frflp to 151 eae-positive strains (including the newly described escherichia albertii) revealed 26 different frflp types that correspond to 20 of the 28 previousl ... | 2006 | 16842363 |
| sensitivity of escherichia albertii, a potential food-borne pathogen, to food preservation treatments. | escherichia albertii is a potential food-borne pathogen because of its documented ability to cause diarrheal disease by producing attachment and effacement lesions. its tolerances to heat (56 degrees c), acid (ph 3.0), and pressure (500 mpa [5 min]) were evaluated and found to be significantly less than those of wild-type e. coli o157:h7. | 2007 | 17468283 |
| role of rna polymerase sigma-factor (rpos) in induction of glutamate-dependent acid-resistance of escherichia albertii under anaerobic conditions. | escherichia albertii is a potential enteric food-borne pathogen with poorly defined genetic and biochemical properties. acid resistance is perceived to be an important property of enteric pathogens, enabling them to survive passage through stomach acidity so that they may colonize the mammalian gastrointestinal tract. we analyzed glutamate-dependent acid-resistance pathway (gdar) in five e. albertii strains that have been identified so far. we observed that the strains were unable to induce gdar ... | 2008 | 18422618 |
| escherichia albertii and hafnia alvei are candidate enteric pathogens with divergent effects on intercellular tight junctions. | attaching-effacing lesion-inducing escherichia albertii and the related, but non-attaching-effacing organism, hafnia alvei, are both implicated as enteric pathogens in humans. however, effects of these bacteria on epithelial cells are not well-characterized. related enteropathogens, including enterohemorrhagic escherichia coli o157:h7, decrease epithelial barrier function by disrupting intercellular tight junctions in polarized epithelia. therefore, this study assessed epithelial barrier functio ... | 2008 | 18930803 |
| characterization of a beta-glucoside operon (bgc) prevalent in septicemic and uropathogenic escherichia coli strains. | escherichia coli strains, in general, do not ferment cellobiose and aryl-beta-d-glucosidic sugars, although "cryptic" beta-d-glucoside systems have been characterized. here we describe an additional cryptic operon (bgc) for the utilization of cellobiose and the aryl-beta-d-glucosides arbutin and salicin at low temperature. the bgc operon was identified by the characterization of beta-glucoside-positive mutants of an e. coli septicemia strain (i484) in which the well-studied bgl (aryl-beta-d-gluc ... | 2009 | 19233952 |
| detection of potentially pathogenic bacteria in the drinking water distribution system of a hospital in hungary. | the drinking water distribution system of a hospital was investigated using standard cultivation techniques, taxon-specific pcrs targeting pathogenic bacteria, denaturing gradient gel electrophoresis, cloning and sequencing. the results obtained verify the higher sensitivity of pcr compared to cultivation for detecting legionella and pseudomonas aeruginosa. moreover, several other opportunistic pathogenic bacteria, such as escherichia albertii, acinetobacter lwoffi and corynebacterium tuberculos ... | 2010 | 19519854 |
| escherichia albertii in wild and domestic birds. | escherichia albertii has been associated with diarrhea in humans but not with disease or infection in animals. however, in december 2004, e. albertii was found, by biochemical and genetic methods, to be the probable cause of death for redpoll finches (carduelis flammea) in alaska. subsequent investigation found this organism in dead and subclinically infected birds of other species from north america and australia. isolates from dead finches in scotland, previously identified as escherichia coli ... | 2010 | 20350378 |
| ability of three dna-based assays to identify presumptive escherichia coli colonies isolated from water by the culture-based mfc agar method. | we tested the ability of three pcr assays, targeting uida and tuf genes to correctly identify escherichia coli colonies isolated from water and we compared them to two ß-glucuronidase-based culture methods (colilert(®) and readycult(®)), in terms of specificity and sensitivity. e. coli isolates recovered on mfc agar were first tested for the presence of the uida positive colonies were presumed to be e. coli. for further characterization, uida-negative colonies were subsequently identified using ... | 2011 | 21420142 |
| Epidemiological investigation of eaeA-positive Escherichia coli and Escherichia albertii strains isolated from healthy wild birds. | Escherichia coli has commonly been associated with diarrheal illness in humans and animals. Recently, E. albertii has been reported to be a potential pathogen of humans and animals and to be carried by wild birds. In the present study, the prevalence and genetic characteristics of intimin-producing E. coli and E. albertii strains were evaluated in wild birds in Korea. Thirty one of 790 Enterobacteriaceae strains from healthy wild birds were positive for the intimin gene (eaeA) and twenty two of ... | 2011 | 22068490 |
| molecular characterization of cytolethal distending toxin gene-positive escherichia coli from healthy cattle and swine in nara, japan. | cytolethal distending toxin (cdt)-producing escherichia coli (ctec) has been isolated from patients with gastrointestinal or urinary tract infection, and sepsis. however, the source of human infection remains unknown. in this study, we attempted to detect and isolate ctec strains from fecal specimens of healthy farm animals and characterized them phenotypically and genotypically. | 2014 | 24742173 |
| first complete genome sequence of escherichia albertii strain kf1, a new potential human enteric pathogen. | escherichia albertii has been recently recognized as an emerging human and bird enteric pathogen. here, we report the first complete chromosome nucleotide sequence of a clinical isolate of e. albertii strain kf1, which may provide information about the pathogenic potential of this new species and the mechanisms of evolution of enteropathogenic escherichia spp. | 2014 | 24482506 |
| defining the genome features of escherichia albertii, an emerging enteropathogen closely related to escherichia coli. | escherichia albertii is a recently recognized close relative of escherichia coli. this emerging enteropathogen possesses a type iii secretion system (t3ss) encoded by the locus of enterocyte effacement, similar to enteropathogenic and enterohemorrhagic e. coli (epec and ehec). shiga toxin-producing strains have also been identified. the genomic features of e. albertii, particularly differences from other escherichia species, have not yet been well clarified. here, we sequenced the genome of 29 e ... | 2015 | 26537224 |
| evaluating the occurrence of escherichia albertii in chicken carcass rinses by pcr, vitek analysis, and sequencing of the rpob gene. | escherichia albertii is a recently described species that has been associated with gastroenteritis in humans and with healthy and ill birds. most recently, it has been identified as the causative agent in a food-borne outbreak in japan. the distribution and clinical importance of e. albertii are not well studied because its importance is unclear. culture methods for clinical isolation frequently miss e. albertii or incorrectly identify it as shigella spp., escherichia coli, or hafnia alvei. this ... | 2015 | 25548040 |
| lambda red-mediated recombineering in the attaching and effacing pathogen escherichia albertii. | the ability to introduce site-specific mutations in bacterial pathogens is essential towards understanding their molecular mechanisms of pathogenicity. this has been greatly facilitated by the genetic engineering technique of recombineering. in recombineering, linear double- or single-stranded dna molecules with two terminal homology arms are electroporated into hyperrecombinogenic bacteria that express a phage-encoded recombinase. the recombinase catalyzes the replacement of the endogenous alle ... | 2016 | 26843851 |
| production of partially phosphorylated myo-inositol phosphates using phytases immobilised on magnetic nanoparticles. | phytases of different origin were covalently bound onto fe3o4 magnetic nanoparticles (12 nm). binding efficiencies of all three phytases were well above 70% relative to the number of aldehyde groups available on the surface of the magnetic nanoparticles. temperature stability for all three phytases was enhanced as a consequence of immobilisation, whereas ph dependence of enzyme activity was not affected. maximum catalytic activity of the immobilised phytases was found at 60°c (rye), 65°c (asperg ... | 2013 | 23747448 |
| escherichia marmotae sp. nov., isolated from faeces of marmota himalayana. | the taxonomic position of a group of seven closely related lactose-negative enterobacterial strains, which were isolated from fresh faecal samples of marmota himalayana collected from the qinghai-tibetan plateau, china, was determined by using a polyphasic approach. cells were gram-reaction-negative, non-sporulating, non-motile, short rods (0.5-1 × 1-2.5 μm). by 16s rrna gene sequences, the representative strain, ht073016(t), showed highest similarity values with escherichia fergusonii atcc 3546 ... | 2015 | 25851592 |
| locus of enterocyte effacement: a pathogenicity island involved in the virulence of enteropathogenic and enterohemorragic escherichia coli subjected to a complex network of gene regulation. | the locus of enterocyte effacement (lee) is a 35.6 kb pathogenicity island inserted in the genome of some bacteria such as enteropathogenic escherichia coli, enterohemorrhagic e.coli, citrobacter rodentium, and escherichia albertii. lee comprises the genes responsible for causing attaching and effacing lesions, a characteristic lesion that involves intimate adherence of bacteria to enterocytes, a signaling cascade leading to brush border and microvilli destruction, and loss of ions, causing seve ... | 2015 | 25710006 |
| escherichia albertii inactivation following l-lactic acid exposure or cooking in ground beef. | escherichia albertii is an emerging foodborne pathogen recovered from young children and adults exhibiting symptoms of gastroenteritis via pathogenesis factors including attaching and effacing lesions, cytolethal distending toxin, and shiga toxin variants. study objectives were to determine e. albertii survival following (i) exposure to lactic acid as a function of solution ph and incubation period and (ii) cooking ground beef patties to different endpoint temperatures. e. albertii was incubated ... | 2016 | 28221930 |
| escherichia albertii, a novel human enteropathogen, colonizes rat enterocytes and translocates to extra-intestinal sites. | diarrhea is the second leading cause of death of children up to five years old in the developing countries. among the etiological diarrheal agents are atypical enteropathogenic escherichia coli (aepec), one of the diarrheagenic e. coli pathotypes that affects children and adults, even in developed countries. currently, genotypic and biochemical approaches have helped to demonstrate that some strains classified as aepec are actually e. albertii, a recently recognized human enteropathogen. studies ... | 2017 | 28178312 |
| isolation and characterization of an escherichia albertii producing three different toxins from a child with diarrhea. | here, we show that a bacterium isolated as a sole pathogen from a child with diarrhea harbor eae and 2 different cytolethal distending toxin genes (cdt) homologous to escherichia coli cdt-i and cdt-ii genes, respectively. the bacterium was originally identified as atypical e. coli by conventional biochemical tests. however, this bacterium was finally identified as e. albertii by multilocus sequence analysis, which is an only method being able to clearly differentiate e. coli from e. albertii. sh ... | 2016 | 27580579 |
| first bacteraemic human infection with escherichia albertii. | the facultative anaerobic gram-negative species escherichia albertii has been isolated from human faeces in gastrointestinal infection and from a range of wild bird species. here we report the first case of a febrile infection associated with e. albertii bacteraemia in a 76-year-old woman with gastric dysplasia. | 2015 | 27257499 |
| prevalence of eae-positive, lactose non-fermenting escherichia albertii from retail raw meat in china. | escherichia albertii is a newly emerging enteric pathogen that has been associated with gastroenteritis in humans. recently, e. albertii has also been detected in healthy and sick birds, animals, chicken meat and water. in the present study, the prevalence and characteristics of the eae-positive, lactose non-fermenting e. albertii strains in retail raw meat in china were evaluated. thirty isolates of such strains of e. albertii were identified from 446 (6·73%) samples, including duck intestines ... | 2016 | 26004066 |
| isolation of escherichia albertii from raw chicken liver in fukuoka city, japan. | | 2015 | 25993974 |
| detection of escherichia albertii from chicken meat and giblets. | escherichia albertii occasionally causes food-borne outbreaks of gastroenteritis in humans; however, little is known about the vehicle of transmission. to screen retail chicken products for the presence of e. albertii, 104 retail chicken products were investigated. portions of enrichment cultures that were pcr-positive for e. albertii (n=3) were sub-cultured on agar medium. only 2 strains obtained from 2 chicken giblet samples were identified as e. albertii by multi locus sequence typing. antimi ... | 2015 | 25754935 |
| shiga toxin 2a in escherichia albertii. | | 2015 | 25653403 |
| nonspecificity of primers for escherichia albertii detection. | | 2014 | 25410570 |
| shiga toxin 2f-producing escherichia albertii from a symptomatic human. | the previously identified shiga toxin (stx) 2f-producing escherichia coli o115:hnm strain f08/101-31, isolated from a symptomatic human, was confirmed to be e. albertii in the present study by whole genome dna-dna hybridizations, by sequencing (cpn60, dnaj, and 16s rrna genes), and by multi-locus sequence typing. the f08/101-31 strain was originally identified as e. coli rather than the relatively new bacterial species e. albertii, which was first described in 2003, because it did not display an ... | 2014 | 24858610 |
| characterization of escherichia fergusonii and escherichia albertii isolated from water. | the aim of this study was to characterize escherichia fergusonii and escherichia albertii isolated from water. | 2014 | 24849008 |
| identification of escherichia albertii as a causative agent of a food-borne outbreak occurred in 2003. | | 2014 | 24647262 |
| an environmental escherichia albertii strain, dm104, induces protective immunity to shigella dysenteriae in guinea pig eye model. | the environmental escherichia albertii strain dm104, which cross-reacts serologically with shigella dysenteriae was assessed for pathogenic properties, immunogenicity, and protective efficacy in different animal models to evaluate it as a vaccine candidate against s. dysenteriae, which causes the severe disease, shigellosis. the dm104 isolate was found to be non-invasive and did not produce any entero- or cyto-toxins. the strain also showed negative results in the mouse lethal activity assay. th ... | 2014 | 24452425 |
| escherichia albertii, a newly emerging enteric pathogen with poorly defined properties. | escherichia albertii is a newly emerging enteric pathogen that has been associated with sporadic infections among humans and birds. selected coliform isolates were screened for allelic variation in 2 housekeeping genes (lysp and mdh) specific for e. albertii. the 48 strains that were identified as e. albertii were tested for 15 virulence markers and biochemical and serogical properties. all e. albertii strains were non-motile, fermented d-glucose (with gas), d-mannitol, and d-mannose, but failed ... | 2013 | 23938305 |
| serological cross-reaction between o-antigens of shigella dysenteriae type 4 and an environmental escherichia albertii isolate. | an environmental freshwater bacterial isolate, dm104, appearing as shigella-like colonies on selective agar plates was found to show strong and specific serological cross-reactivity with shigella dysenteriae type 4. biochemical identification according to the analytical profile index, molecular serotyping by restriction of the amplified o-antigen gene cluster (rfb-rflp), together with phylogenetic analysis of the 16s rrna gene and multi-locus sequence analysis, identified the isolate as escheric ... | 2013 | 23748967 |
| antibiotic resistance and growth of the emergent pathogen escherichia albertii on raw ground beef stored under refrigeration, abuse, and physiological temperature. | escherichia albertii is an emerging gram-negative facultative rod that has been implicated in multiple cases of human diarrheal disease, particularly in young children. when biochemical and other typing methods have been used, this organism has often been misidentified due to similarities with other members of the family enterobacteriaceae. isolates have been reported to be capable of producing attachment and effacement lesions via the synthesis of intimin, cytolethal distending toxin, and a var ... | 2013 | 23317867 |
| human gastroenteritis outbreak associated with escherichia albertii, japan. | although escherichia albertii is an emerging intestinal pathogen, it has been associated only with sporadic human infections. in this study, we determined that a human gastroenteritis outbreak at a restaurant in japan had e. albertii as the major causative agent. | 2013 | 23260717 |
| isolation and identification of escherichia albertii from a patient in an outbreak of gastroenteritis. | a microbial strain harboring the eae gene, which is known as the virulence gene of enteropathogenic escherichia coli (epec) and most enterohemorrhagic e. coli, was isolated from a patient in a gastroenteritis outbreak that occurred in 22 patients in akita prefecture, japan, in november 2011. the biochemical characteristics of the isolate were more similar to those of a novel escherichia sp., e. albertii than e. coli. partial 16s rrna gene sequences of the isolate were identical to those of a cer ... | 2012 | 22627300 |
| clinical significance of escherichia albertii. | discriminating escherichia albertii from other enterobacteriaceae is difficult. systematic analyses showed that e. albertii represents a substantial portion of strains currently identified as eae-positive escherichia coli and includes shiga toxin 2f-producing strains. because e. albertii possesses the eae gene, many strains might have been misidentified as enterohemorrhagic or enteropathogenic e. coli. | 2012 | 22377117 |
| structures and gene clusters of the o-antigens of escherichia albertii o3, o4, o6, and o7. | the o-specific polysaccharides (opss) called o-antigens were obtained by mild acid degradation of the lipopolysaccharides of escherichia albertii serotypes o3, o4, o6, and o7 and studied by sugar analysis along with 1d and 2d (1)h and (13)c nmr spectroscopy. the following structure was established for the ops of e. albertii o4, which, to our knowledge, is unique among known bacterial polysaccharide structures: →2)-α-l-rhap-(1 → 2)-α-l-fucp-(1 → 2)-β-d-galp-(1 → 3)-α-d-galpnac-(1 → 3)-β-d-glcpnac ... | 2017 | 28672166 |
| multiplex polymerase chain reaction for identification of escherichia coli, escherichia albertii and escherichia fergusonii. | escherichia coli, escherichia albertii, and escherichia fergusonii are closely related bacteria that can cause illness in humans, such as bacteremia, urinary tract infections and diarrhea. current identification strategies for these three species vary in complexity and typically rely on the use of multiple phenotypic and genetic tests. to facilitate their rapid identification, we developed a multiplex pcr assay targeting conserved, species-specific genes. we used the daydreamer™ (pattern genomic ... | 2017 | 28599915 |
| protection against shigellosis caused by shigella dysenteriae serotype 4 in guinea pigs using escherichia albertii dm104 as a live vaccine candidate strain. | recently, we reported the induction of protective immunity by environmental escherichia albertii strain dm104 against shigella dysenteriae in guinea pig model. in this study, we assessed three different immunization routes, such as intranasal, oral, and intrarectal routes, and revealed differences in immune responses by measuring both the serum igg and mucosal iga antibody titers. protective efficacy of different routes of immunization was also determined by challenging immunized guinea pigs aga ... | 2017 | 28597684 |
| structure and gene cluster of the o-antigen of escherichia albertii o1 resembling the o-antigen of pseudomonas aeruginosa o5. | the o-specific polysaccharide (o-antigen) was obtained by mild acid degradation of the lipopolysaccharide of escherichia albertii serotype o1 strain sp20140089 and studied by sugar analysis along with 1d and 2d (1)h and (13)c nmr spectroscopy. the following structure was established for the trisaccharide repeating unit of the o-polysaccharide: →4)-β-d-manpnac3naca-(1 → 4)-β-d-glcpnam3naca-(1 → 3)-α-d-glcpnac-(1→ where mannac3naca and glcnam3naca indicate 2,3-diacetamido-2,3-dideoxymannuronic aci ... | 2017 | 28494314 |