| genomic sequence analyses of segments 1 to 6 of dendrolimus punctatus cytoplasmic polyhedrosis virus. | the complete nucleotide sequences of genomic segments s1 to s6 from dendrolimus punctatus cypovirus 1 (dpcpv-1) have been determined. each segment of s1 to s6 possess a single open reading frame. conserved motifs 5' (aguaa) and 3'(guuagcc) were found at the ends of each segment. comparison of the proteins of dpcpv with those of other members in the family reoviridae lead us to suggest that s1, s3, s4 and s6 encode the viral structural protein vp1, vp2, vp3 and vp4, respectively. s5 encoded viral ... | 2003 | 12827465 |
| nucleotide sequences of genome segments s6, s7 and s10 of dendrolimus punctatus cypovirus 1. | the nucleotide sequences of genome segments s6, s7 and s10 of dendrolimus punctatus cypovirus 1 hunan i (dpcpv-hn(i)) and dpcpv-hn(i)-se(3) (dpcpv-hn(i) passed three times in spodoptera exigua) were determined. segment s10 was 944 nucleotides in length and encoded a polyhedrin of 248 amino acids (28,439 da). only two nucleotide mutations were found between dpcpv-hn(i) s10 and dpcpv-hn(i)-se3 s10, and the deduced amino acid sequences of the polyhedrin proteins were identical. segment s7, 1 501 nu ... | 2004 | 14689283 |
| characterization of the rna-binding domain in the dendrolimus punctatus cytoplasmic polyhedrosis virus nonstructural protein p44. | dendrolimus punctatus cytoplasmic polyhedrosis virus (dpcpv-1) belongs to the cypovirus genus in the reoviridae family. the orf of genome segment 8 (s8) of dpcpv-1 was cloned into vector pmal-c2x and used to express a 44kda protein (p44) in e. coli, which was detected by western blotting. the gel mobility shift assays showed that p44 had ssrna-binding activity. competitive assay indicated that this protein only bind to ssrna and could not interact with dna and dsrna. the binding of p44 to ssrna ... | 2005 | 16023751 |
| mapping the rna-binding domain on the dpcpv vp4. | the rna-binding properties of vp4 protein of dendrolimus punctatus cytoplasmic polyhedrosis virus (dpcpv) vp4 were analyzed. vp4 was expressed in e. coli and assayed for rna binding activity by gel mobility shift assay. vp4 was found to bind rna (ssrna and dsrna) in a sequence-independent manner, but did not interact with dna. to identify the domain(s) of the protein important for rna binding, a number of deletions were made and tested by gel mobility shift assays and northwestern blot. the cent ... | 2006 | 16195791 |
| identification of transmembrane domain of a membrane associated protein ns5 of dendrolimus punctatus cytoplasmic polyhedrosis virus. | we examined the intracellular localization of ns5 protein of dendrolimus punctatus cytoplasmic polyhedrosis virus (dpcpv) by expressing ns5-gfp fusion protein and proteins from deletion mutants of ns5 in baculovirus recombinant infected insect spodoptera frugiperda (sf-9) cells. it was found that the ns5 protein was present at the plasma membrane of the cells, and that the n-terminal portion of the protein played a key role in the localization. a transmembrane region was identified to be present ... | 2006 | 16889685 |
| characterisation and partial sequence analysis of two novel cypoviruses isolated from the winter moth operophtera brumata (lepidoptera: geometridae). | the complete nucleotide sequences of genomic segments s5 to s10 from operophtera brumata cypovirus 18 (opbucpv18), and the complete nucleotide sequences of genomic segments s2, s5, s9 and s10 from operophtera brumata cypovirus 19 (opbucpv19) have been determined. each genome segment contained a single open reading frame (orf). conserved motifs 5' (aguaaa....guuagcu) 3' were found at the ends of each segment of opbucpv18, whilst conserved motifs 5' (aacaaa....uuugc) 3' were found at each segment ... | 2007 | 17546493 |
| molecular characterization of segments 7-10 of dendrolimus punctatus cytoplasmic polyhedrosis virus provides the complete genome. | the nucleotide sequences of genomic segments s7-s10 from dendrolimus punctatus cypovirus strain hunan (dpcpv-hn) have been determined. this provides the complete genome sequences of dpcpv-hn. each segment of s7-s10 possess a single segment each. homology searches showed that the nucleotide sequences and the deduced amino acid sequences of dpcpv s7-10 had high level of identities with those of bombyx mori cypovirus (bmcpv) s7-10, respectively. while the amino acid sequences of the proteins encode ... | 2003 | 12837553 |