| high-throughput gender identification of accipitridae eagles with real-time pcr using taqman probes. | the objective was to develop high-throughput gender identification of eagles. based on blast and alignment analyses, the chd-z and chd-w sequences of nine species of eagles were highly homologous with spilornis cheela hoya (s. c. hoya); therefore, taqman probes were designed to target their chd-zw-common and chd-w-specific regions. in s. c. hoya, genders were identified using taqman-based, real-time pcr (amplified by p2/p8 primers); this method was validated with anatomically confirmed controls ... | 2008 | 18440628 |
| avian pox infection in a free-living crested serpent eagle (spilornis cheela) in southern taiwan. | avian pox viruses (apvs) have been reported to cause infection in diverse avian species worldwide. herein we report the first case of apv infection in a free-living bird, a subadult crested serpent eagle (spilornis cheela), in taiwan. in addition to the typical wart-like lesions distributed on the cere, eyelid, and face, there were also yellowish nodules below the tongue and on the hard palate. phylogenetic analysis of the 4b core protein gene showed that the apv is very close to that found in w ... | 2011 | 21500652 |
| helminth fauna of a japanese golden eagle, aquila chrysaetos japonica. | a japanese golden eagle, aquila chrysaetos japonica, was found dead in nagano prefecture pb 399-8200, japan, and subjected to necropsy. the necropsy revealed that the entire length of the intestine was filled with several masses of intestinal parasites. the recovered helminths were identified as one digenean trematode species, neodiplostomum reflexum; two species of nematodes, synhimantus sp. and larvae of porrocaecum sp.; and a single species of acanthocephala, centrorhynchus sp. digenea and ac ... | 2012 | 23272374 |
| high-throughput avian molecular sexing by sybr green-based real-time pcr combined with melting curve analysis. | combination of chd (chromo-helicase-dna binding protein)-specific polymerase chain reaction (pcr) with electrophoresis (pcr/electrophoresis) is the most common avian molecular sexing technique but it is lab-intensive and gel-required. gender determination often fails when the difference in length between the pcr products of chd-z and chd-w genes is too short to be resolved. | 2008 | 18269737 |
| quantitative and qualitative morphologic, cytochemical and ultrastructural characteristics of blood cells in the crested serpent eagle and shikra. | the crested serpent eagle (spilornis cheela) is a bird of prey found in the tropical rain forest in thailand. the shikra (accipiter badius) is a sparrow hawk and common resident in thailand. blood samples from 9 crested serpent eagles and 12 shikras were obtained from september 2010 to november 2014. they were clinically healthy and negative for blood parasites detectable by light microscopy and molecular techniques (partial cytochrome b gene for avian malaria and partial 18s rrna gene for trypa ... | 2015 | 26563029 |
| use of random amplified polymorphic dna to identify several novel markers for sex identification in the crested serpent eagle and crested goshawk. | the crested serpent eagle (spilornis cheela hoya) has no distinct sexual dimorphic traits. in the current study, we report the results of an ee0.6 (ecori 0.6-kb fragment) sequence applied to s. cheela hoya and a novel random amplified polymorphic dna (rapd) marker that can be used to sex individuals within the species s. cheela hoya and accipiter trivigatus formosae (crested goshawk). we used sex-specific primers for the avian chd1 (chromo-helicase-dna-binding 1) gene and the ee0.6 sequence in p ... | 2009 | 19560806 |
| validation of spilornis cheela hoya taqman probes for potential gender identification of many accipitridae species. | the objective of this study was to test the hypothesis that genders of accipitridae species, with the same or similar sequences to our previously proposed spilornis cheela hoya (s. c. hoya) chromo-helicase-dna binding protein (chd)-w-specific and chd-zw-common taqman probes, can be successfully determined. eight species of accipitridae with known genders were collected. after pcr, ta cloning, sequencing, and alignment analyses, sequence length differences of griffiths p2/p8 pcr amplicons between ... | 2010 | 19959218 |
| some species of centrorhynchus lühe, 1911 (acanthocephala: centrorhynchidae) from the collection of the natural history museum, london. | seven species of centrorhynchus lühe, 1911 are present in the parasitic worms collection of the natural history museum, london: c. aluconis (müller, 1780) lühe, 1911 from strix aluco linnaeus in great britain; c. buteonis (schrank, 1788) kostylev, 1914 from accipiter virgatus (temminck) (new host record) in sri lanka (new geographical record); c. clitorideus (meyer, 1931) golvan, 1956 from athene brama (temminck) (new host record) in india; c. crotophagicola schmidt and neiland, 1966 (encysted j ... | 2005 | 16167121 |
| dna barcodes of philippine accipitrids. | dna barcoding is a molecular method that rapidly identifies an individual to a known taxon or its closest relative based on a 650-bp fragment of the cytochrome c oxidase subunit i (coi). in this study, dna barcodes of members of the family accipitridae, including haliastur indus (brahminy kite), haliaeetus leucogaster (white-bellied sea eagle), ichthyophaga ichthyaetus (grey-headed fish eagle), spilornis holospilus (crested serpent-eagle), spizaetus philippensis (philippine hawk-eagle), and pith ... | 2011 | 21429130 |
| complete mitochondrial genome of the spilornis cheela (falconiformes, accipitridae): comparison of s. cheela and spizaetus alboniger. | here, we sequenced the complete mitochondrial genome of spilornis cheela (falconiformes, accipitridae), which is considered as endemic raptor species and listed in the second category of national key protected wild animals in china. the genome is 18,291 bp in size. its gene arrangement pattern was identical with that of spizaetus alboniger. we compared the mitochondrial genome of s. cheela with that of s. alboniger. nucleotide sequence similarity between the two whole mitochondrial genomes was 8 ... | 2013 | 23316785 |
| hematocrit and plasma chemistry values in adult collared scops owls (otus lettia) and crested serpent eagles (spilornis cheela hoya). | in this study, we report hematocrit and plasma chemistry values for adult captive collared scops owls (otus lettia) and crested serpent eagles (spilornis cheela hoya). in particular, we address the gender-specific differences within these values. we measured hematocrit (hct) and plasma chemistry values for uric acid (ua), plasma urea nitrogen (bun), total protein (tp), albumin (alb), glucose (glu), cholesterol (cho), triglyceride (tg), aspartate aminotransferase (ast), alanine aminotransferase ( ... | 2012 | 22446394 |
| anesthesia with isoflurane and sevoflurane in the crested serpent eagle (spilornis cheela hoya): minimum anesthetic concentration, physiological effects, hematocrit, plasma chemistry and behavioral effects. | the initial goal of this study was to determine the minimum anesthetic concentration (mac) for isoflurane (iso) and sevoflurane (sevo) for the crested serpent eagle. next, we compared the anesthetic effects of each on the physiological effects, hematocrit, plasma chemistry values and behavior in spontaneously breathing captive adult crested serpent eagles. sixteen eagles were randomly allocated to two groups for anesthesia with iso (n=8) or sevo (n=8). first, we measured the mac values of iso an ... | 2013 | 23955396 |
| an improved pcr method for gender identification of eagles. | eagles are sexually monomorphic and therefore it is difficult to determine their gender, which is a crucial need for management purposes. in this study, we have developed an improved gender identification method by exploiting length differences between the chromo-helicase-dna binding protein (chd)-z and chd-w genes of spilornis cheela hoya. by comparing dna sequences for chd-w and chd-z from 10 species of falconiformes eagles we designed universal gender identification pcr primers that exploit d ... | 2008 | 18385011 |
| frequent detection of anticoagulant rodenticides in raptors sampled in taiwan reflects government rodent control policy. | anticoagulant rodenticides (ars) are known to cause extensive secondary exposure in top predators in europe and north america, but there remains a paucity of data in asia. in this study, we collected 221 liver samples from 21 raptor species in taiwan between 2010 and 2018. most birds were recovered from rescue organizations, but some free-ranging individuals were obtained from bird-strike prevention measures at airports. ars were detected in 10 species and more than half of the total samples. co ... | 2019 | 31326797 |
| complete mitochondrial genome sequence of the himalayan griffon, gyps himalayensis (accipitriformes: accipitridae): sequence, structure, and phylogenetic analyses. | this is the first study to describe the mitochondrial genome of the himalayan griffon, gyps himalayensis, which is an old world vulture belonging to the family accipitridae and occurring along the himalayas and the adjoining tibetan plateau. its mitogenome is a closed circular molecule 17,381 bp in size containing 13 protein-coding genes, 22 trna coding genes, two rrna-coding genes, a control region (cr), and an extra pseudo-control region (ccr) that are conserved in most accipitridae mitogenome ... | 2019 | 31410282 |