| complete sequence determination and genetic analysis of banna virus and kadipiro virus: proposal for assignment to a new genus (seadornavirus) within the family reoviridae. | arboviruses with genomes composed of 12 segments of double-stranded (ds) rna have previously been classified as members or probable members of the genus coltivirus within the family reoviridae: a number of these viruses have been isolated in north america and europe and are serologically and genetically related to colorado tick fever virus, the coltivirus type species. these isolates constitute subgroup a of the coltiviruses. the complete genome sequences are now presented of two asian arbovirus ... | 2000 | 10811934 |
| genus coltivirus (family reoviridae): genomic and morphologic characterization of old world and new world viruses. | we report a genomic and morphologic study of the european eyach (eya) virus (genus coltivirus, family reoviridae) and a comparative analysis with the american colorado tick fever (ctf) virus (the type species of the genus). the previously established, but distant, antigenic relationship between these viruses was strengthened by genetic findings (presence of cognate genes, amino acid identity between 55 and 88%, similar conserved terminal motifs, suspected read-through phenomenon in segment 9 of ... | 2002 | 11958454 |
| recombinant vp9-based enzyme-linked immunosorbent assay for detection of immunoglobulin g antibodies to banna virus (genus seadornavirus). | banna virus (bav, genus seadornavirus, family reoviridae) is an arbovirus suspected to be responsible for encephalitis in humans. two genotypes of this virus are distinguishable: a (chinese isolate, bav-ch) and b (indonesian isolate, bav-in6969) which exhibit only 41% amino-acid identity in the sequence of their vp9. the vp7 to vp12 of bav-ch and vp9 of bav-in6969 were expressed in bacteria using pgex-4t-2 vector. vp9 was chosen to establish an elisa for bav, based mainly on two observations: (i ... | 2004 | 14715307 |
| the structure and function of the outer coat protein vp9 of banna virus. | banna virus (bav: genus seadornavirus, family reoviridae) has a double-shelled morphology similar to rotavirus and bluetongue virus. the structure of bav outer-capsid protein vp9 was determined by x-ray crystallography at 2.6 a resolution, revealing a trimeric molecule, held together by an n-terminal helical bundle, reminiscent of coiled-coil structures found in fusion-active proteins such as hiv gp41. the major domain of vp9 contains stacked beta sheets with marked structural similarities to th ... | 2005 | 15642258 |
| identification and functional analysis of vp3, the guanylyltransferase of banna virus (genus seadornavirus, family reoviridae). | banna virus (bav) particles contain seven structural proteins: vp4 and vp9 form an outer-capsid layer, whilst the virus core contains three major proteins (vp2, vp8 and vp10) and two minor proteins (vp1 and vp3). sequence analysis showed that vp3 contains motifs [kx(i/v/l)s] and (hx(n)h) that have previously been identified in the guanylyltransferases of other reoviruses. incubation of purified bav-ch core particles with [alpha-32p]gtp resulted in exclusive covalent labelling of vp3, demonstrati ... | 2005 | 15784908 |
| structural organization of an encephalitic human isolate of banna virus (genus seadornavirus, family reoviridae). | banna virus (bav) is the type species of the genus seadornavirus within the family reoviridae. the chinese bav isolate (bav-ch), which causes encephalitis in humans, was shown to have a structural organization and particle morphology reminiscent of that of rotaviruses, with fibre proteins projecting from the surface of the particle. intact bav-ch virus particles contain seven structural proteins, two of which (vp4 and vp9) form the outer coat. the inner (core) particles contain five additional p ... | 2005 | 15784909 |
| liao ning virus, a new chinese seadornavirus that replicates in transformed and embryonic mammalian cells. | seadornaviruses are emerging arboviral pathogens from the south-east of asia. the genus seadornavirus contains two distinct species, banna virus (bav) isolated from humans with encephalitis and kadipiro virus. bav replicates within insect cells and mice but not in cultured mammalian cells. here, the discovery of liao ning virus (lnv), a new seadornavirus from the aedes dorsalis mosquito, which was completely sequenced and was found to be related to bav and kadipiro virus, is reported. two seroty ... | 2006 | 16361432 |
| [detection of sindbis virus-specific nucleic acid with sybr green i real time pcr assay]. | to develop a rapid, specific and sensitive method for detecting sindbis virus (sinv) with sybr green i real time pcr. | 2005 | 16415993 |
| [laboratory testing of specimens from patients with viral encephalitis from some regions of china]. | to understand the viral etiology of viral encephalitis in china by detecting igm antibody and viral rna in the clinical samples of patients from some provinces of china by enzyme-linked immunosorbent assay and polymerase chain reaction. | 2008 | 18574526 |
| [0507js60 virus isolated in xinjiang was identified as liaoning virus]. | 0507js60 virus was isolated from a pool of culex sp. collected in kashi, xinjiang, which could be propagated stably on c6/36 cells and caused cytopathic effects continuously. viral particles had no envelope and appeared round with diameter of about 55nm (n = 10). capsomeres on the surface of capsid were clearly visible. electrophoresis of viral genome showed a profile of 12 double stranded rna (dsrna) segments. sequencing of the twelfth segment revealed the length of 760bp (genbank id: fj157354) ... | 2008 | 19226952 |
| liao ning virus in china. | abstract: background: liao ning virus is in the genus seadornavirus within the family reoviridae and has a genome composed of 12 segments of double-stranded rna (dsrna). it is transmitted by mosquitoes and only isolated in china to date and it is the only species within the genus seadornavirus which was reported to have been propagated in mammalian cell lines. in the study, we report 41 new isolates from northern and southern xinjiang uygur autonomous region in china and describe the phylogeneti ... | 2011 | 21649929 |