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enterobacter kobei sp. nov., a new species of the family enterobacteriaceae resembling enterobacter cloacae.the name enterobacter kobei sp. nov. is proposed for a group of organisms referred to as nih group 21 at the national institute of health, tokyo. the members of this species are gram-negative, motile rods conforming to the definition of the family enterobacteriaceae. the dna relatedness of 23 strains of nih group 21 to the representative proposed as the type strain of this species averaged 82% at 70 degrees c, whereas the relatedness to other species within the family enterobacteriaceae was less ...19968824173
population genetics of the nomenspecies enterobacter cloacae.the genetic heterogeneity of the nomenspecies enterobacter cloacae is well known. enterobacter asburiae, enterobacter cancerogenus, enterobacter dissolvens, enterobacter hormaechei, enterobacter kobei, and enterobacter nimipressuralis are closely related to it and are subsumed in the so-called e. cloacae complex. dna-dna hybridization studies performed previously identified at least five dna-relatedness groups of this complex. in order to analyze the genetic structure and the phylogenetic relati ...200312957918
gamma proteobacteria can nodulate legumes of the genus hedysarum.the bacteria hosted in the root nodules of the three mediterranean wild legume species hedysarum carnosum, hedysarum spinosissimum subsp. capitatum, and hedysarum pallidum, growing in native stands in different habitats in algeria were isolated. bacteria were recovered on yeast-mannitol-agar or on minimal media from a total of 52 nodules. isolates were analyzed by amplified ribosomal dna restriction analysis (ardra) using the enzyme cfoi, and further sorted by rapd fingerprinting. a total of ten ...200415368852
reassignment of enterobacter dissolvens to enterobacter cloacae as e. cloacae subspecies dissolvens comb. nov. and emended description of enterobacter asburiae and enterobacter kobei.the taxonomic position of enterobacter dissolvens was re-evaluated based on the analysis of the type strain atcc 23373t and three clinical isolates. the strains were assigned to the genetic cluster of the species by phylogenetic sequence analysis in the frame of a recent population genetic study. the relatedness of e. dissolves to the other species of the e. cloacae complex was analyzed by dna-dna hybridization studies based on melting profiles in microplates. the genetic cluster of e. dissolven ...200515900966
nosocomial urosepsis caused by enterobacter kobei with aberrant phenotype.enterobacter kobei is the species of the enterobacter cloacae complex, which is phenotypically most closely related to the species e. cloacae. this is the first report of infection caused by a new biotype of e. kobei. a patient with a history of urinary bladder operation developed a urosepsis with an enterobacter isolate displaying the e. cloacae phenotype. the isolate was classified to the species e. kobei by sequence analysis of the 16s-rdna, 4 protein-coding genes and enterobacterial repetiti ...200516168611
bacteria colonizing root nodules of wild legumes exhibit virulence-associated properties of mammalian pathogens.bacteria not proficient in nitrogen fixing symbiosis were proven able to invade root nodules of three wild legumes of the genus hedysarum in algeria and to be multiplying in these in place of the natural rhizobium symbionts. the involved species featured taxa known as human pathogens including: enterobacter cloacae, enterobacter kobei, escherichia vulneris, pantoea agglomerans and leclercia adecarboxylata. a direct screening of the phenotypic determinants of virulence using human cultured cells ...201019916054
comparison of maldi tof with conventional identification of clinically relevant bacteria.a completely new approach to diagnose microbial agents at least one day earlier based on mass spectrometric analysis becomes possible in the microbiology laboratory: maldi tof: matrix-assisted laser desorption/ionisation time-of-flight. comparison between results of the new procedure with those obtained by conventional testing is mandatory.201020924806
resistance patterns and integron cassette arrays of enterobacter cloacae complex strains of human origin.the aim of this research was to analyse the resistance patterns and characterize the distribution and genetic content of resistance integrons within enterobacter cloacae complex strains originating from hospitalized patients. the strains were included in the e. cloacae complex study following sequence analysis of the hsp60 gene. the determination of resistance towards eight classes of antimicrobials was followed by pcr detection of integrons and analyses of the size and sequences of their variab ...201121330416
a dual approach employing maldi-tof ms and real-time pcr for fast species identification within the enterobacter cloacae complex.a real-time pcr procedure targeting the gene of the molecular cochaperon dnaj (dnaj) was developed for specific detection of strains belonging to the enterobacter cloacae group. the inclusivity and exclusivity of the real-time pcr assay were assessed with seven reference strains of e. cloacae, 12 other enterobacter species and 41 non-enterobacter strains. inclusivity as well as exclusivity of the duplex real-time pcr was 100%. in contrast, resolution of matrix-assisted laser desorption ionizatio ...201122150997
enterobacter cloacae complex: clinical impact and emerging antibiotic resistance.species of the enterobacter cloacae complex are widely encountered in nature, but they can act as pathogens. the biochemical and molecular studies on e. cloacae have shown genomic heterogeneity, comprising six species: enterobacter cloacae, enterobacter asburiae, enterobacter hormaechei, enterobacter kobei, enterobacter ludwigii and enterobacter nimipressuralis, e. cloacae and e. hormaechei are the most frequently isolated in human clinical specimens. phenotypic identification of all species bel ...201222827309
enumeration and identification of coliform bacteria injured by chlorine or fungicide mixed with agricultural water.chemical sanitizers may induce no injury (bacteria survive), sublethal injury (bacteria are injured), or lethal injury (bacteria die). the proportion of coliform bacteria that were injured sublethally by chlorine and fungicide mixed with agricultural water (pond water), which was used to dilute the pesticide solution, was evaluated using the thin agar layer (tal) method. in pure cultures of enterobacter cloacae , escherichia coli , and e. coli o157:h7 (representing a human pathogen), the percent ...201628221856
comparison of bacterial genome assembly software for minion data and their applicability to medical microbiology.translating the oxford nanopore minion sequencing technology into medical microbiology requires on-going analysis that keeps pace with technological improvements to the instrument and release of associated analysis software. here, we use a multidrug-resistant enterobacter kobei isolate as a model organism to compare open source software for the assembly of genome data, and relate this to the time taken to generate actionable information. three software tools (pbcr, canu and miniasm) were used to ...201628348876
emergence of a novel enterobacter kobei clone carrying chromosomal-encoded ctx-m-12 with diversified pathogenicity in northeast china. 201728243445
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