Publications

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survival of gfp-tagged antagonistic bacteria in the rhizosphere of tomato plants and their effects on the indigenous bacterial community.the survival and colonization patterns of pseudomonas putida prd16 and enterobacter cowanii prf116 in the rhizosphere of greenhouse-grown tomato plants and the effects of their inoculation on the indigenous bacterial community were followed by selective plating, molecular fingerprinting, and confocal laser scanning microscopy (clsm) over 3 weeks. both strains, which showed in vitro antagonistic activity against ralstonia solanacearum, were previously tagged with gfp. seed and root inoculation we ...200616629751
halotolerance and survival kinetics of lactic acid bacteria isolated from jalapeño pepper (capsicum annuum l.) fermentation.the microbiota associated with spontaneous fermentation of vegetables in a saline substrate may represent an important group of bacteria in the food industry. in this work, the lactic acid bacteria (lab) weissella cibaria, lactobacillus plantarum, lactobacillus paraplantarum, and leuconostoc citreum were identified by partial 16s rrna gene sequence analysis. in addition, entophytic bacteria such as pantoea eucalypti, pantoea anthophila, enterobacter cowanii, and enterobacter asburiae were detect ...201425039289
effects of yeasts and bacteria on the levels of folates in rye sourdoughs.fermentation of rye dough is often accompanied with an increase in folate content. in this study, three sourdough yeasts, candida milleri cbs 8195, saccharomyces cerevisiae ts 146, and torulaspora delbrueckii ts 207; a control, baker's yeast s. cerevisiae alko 743; and four lactobacillus spp., l. acidophilus tsb 262, l. brevis tsb 307, l. plantarum tsb 304, and l. sanfranciscensis tsb 299 originally isolated from rye sourdough were examined for their abilities to produce or consume folates. the ...200616213050
enterobacter radicincitans sp. nov., a plant growth promoting species of the family enterobacteriaceae.a plant growth promoting bacterial isolate (d5/23t) from the phyllosphere of winter wheat, able to fix atmospheric nitrogen and to produce auxines and cytokinins was investigated in a polyphasic taxonomy approach. phylogenetic analyses using the 16s rrna gene sequence of the strain clearly indicated that the strain belonged to the family enterobacteriaceae, most closely related to enterobacter cloacae with 99.0% and enterobacter dissolvens with 98.5% sequence similarity. phylogenetic analysis de ...200515900968
enterobacter cowaniisp. nov., a new species of the family enterobacteriaceae.the name enterobacter cowanii sp. nov. is proposed for a group of organisms referred to as nih group 42. members of this species are gram-negative, motile rods conforming to the definition of the family enterobacteriaceae. the dna relatedness of nine strains of nih group 42 to the proposed type strain of this species averaged 85% at 70 degrees c, whereas the relatedness to other species within the family enterobacteriaceae was less than 38%. because the dna relatedness (5-38%) is closer to speci ...200011080391
isolation and identification of e. cowanii from powdered infant formula in nicu and determination of antimicrobial susceptibility of isolates.enterobacter cowanii is a genus of common gram-negative, facultatively anaerobic, rod-shaped, non-spore-forming bacterium of the enterobacteriaceae family. this organism can be potentially a powdered infant milk formula-borne opportunistic pathogen. the aim of this study was to isolate and identify e. cowanii from consumed powdered infant formula milk (pif) in intensive care units (nicu) and to determine antimicrobial susceptibility patterns of this bacterium.201425562018
new report of additional enterobacterial species causing wilt in west bengal, india.ralstonia solanacearum is known to be the most prominent causal agent of bacterial wilt worldwide. it has a wide host range comprising solanaceous and nonsolanaceous plants. typical symptoms of the disease are leaf wilt, browning of vascular tissues, and collapsing of the plant. with the objective of studying the diversity of pathogens causing bacterial wilt in west bengal, we collected samples of diseased symptomatic crops and adjacent symptomatic and asymptomatic weeds from widespread location ...201526040797
culture-dependent and -independent methods to investigate the predominant microorganisms associated with wet processed coffee.the fermentation process of yunnan arabica coffee is a typical wet fermentation. its excellent quality is closely related to microbes in the process of fermentation. the purpose of this study was to isolate and identify the microorganisms in the wet method of coffee processing in yunnan province, china. microbial community structure and dominant bacterial species were evaluated by traditional cultivated separation method and pcr-dgge technology, and were further analyzed in combination with the ...201627113591
comparison of microbial diversity of edible flowers and basil grown with organic versus conventional methods.the consumption and use of edible flowers as food is growing; however, no study has been conducted to evaluate their role in the cause of food-borne illness or in food safety. recent food-borne outbreaks traced to fresh herbs have raised concern about their processing and handling. basil, one of the most commonly used fresh herbs, has been identified as a source of food-borne illness. baseline assessments of microflora were performed, and the microbial diversity between growing methods (organic ...201021076485
enterobacter arachidis sp. nov., a plant-growth-promoting diazotrophic bacterium isolated from rhizosphere soil of groundnut.a methylotrophic nitrogen-fixing bacterial strain, ah-143(t), isolated from the rhizosphere soil of field-grown groundnut was analysed by a polyphasic taxonomic approach. comparative 16s rrna gene sequence analysis combined with rpob gene sequence analysis allocated strain ah-143(t) to the family enterobacteriaceae, with enterobacter radicincitans and enterobacter cowanii as the closest relatives. the strain is gram-stain-negative, non-spore-forming, aerobic and motile, having straight rod-shape ...201019684326
isolation of enterobacter cowanii from eucalyptus showing symptoms of bacterial blight and dieback in uruguay.this study was performed to identify bacterial strains isolated simultaneously with pantoea species from eucalyptus trees showing symptoms of bacterial blight and dieback in uruguay.200919674289
enterobacter turicensis sp. nov. and enterobacter helveticus sp. nov., isolated from fruit powder.four gram-negative, facultatively anaerobic, non-spore-forming isolates of coccoid rods were obtained from fruit powder and investigated in a polyphasic taxonomic study. comparative 16s rrna gene sequence analysis allocated the isolates to the family enterobacteriaceae. their phylogenetic position within the family enterobacteriaceae was confirmed by rpob sequence analysis and as the highest rpob sequence similarities were obtained with enterobacter radicincitans, enterobacter cowanii and entero ...200717392213
cloning of four genes involved in limonene hydroxylation from enterobacter cowanii 6l.genes encoding proteins responsible for limonene catabolism were cloned from a limonene-degrading microorganism, enterobacter cowanii 6l, which was isolated from citron (citrus junos) peel. the 8.6, 4.7, and 7.7 kb fragments (cd3, cd4, and cd6) of e. cowanii 6l chromosomal dna that confer to e. coli the ability to grow on limonene have been cloned and their corresponding dna sequences were determined. nine open reading frames (orfs) were identified, and the four orfs (921 bp of cd3-2; 1515 bp of ...200718051329
taxonomic evaluation of the genus enterobacter based on multilocus sequence analysis (mlsa): proposal to reclassify e. nimipressuralis and e. amnigenus into lelliottia gen. nov. as lelliottia nimipressuralis comb. nov. and lelliottia amnigena comb. nov., respectively, e. gergoviae and e. pyrinus into pluralibacter gen. nov. as pluralibacter gergoviae comb. nov. and pluralibacter pyrinus comb. nov., respectively, e. cowanii, e. radicincitans, e. oryzae and e. arachidis into kosakonia gen. nov. as kosakonia cowanii comb. nov., kosakonia radicincitans comb. nov., kosakonia oryzae comb. nov. and kosakonia arachidis comb. nov., respectively, and e. turicensis, e. helveticus and e. pulveris into cronobacter as cronobacter zurichensis nom. nov., cronobacter helveticus comb. nov. and cronobacter pulveris comb. nov., respectively, and emended description of the genera enterobacter and cronobacter.the taxonomy of enterobacter has a complicated history, with several species transferred to and from this genus. classification of strains is difficult owing to its polyphyletic nature, based on 16s rrna gene sequences. it has been previously acknowledged that enterobacter contains species which should be transferred to other genera. in an attempt to resolve the taxonomy of enterobacter, mlsa based on partial sequencing of protein-encoding genes (gyrb, rpob, infb and atpd) was performed on the t ...201323632228
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