| use of molecular hybridization to detect type d retrovirus markers in rhesus placentas and other tissues. | we have shown previously that approximately 20% of the mason-pfizer virus (mpv) genome is present as endogenous provirus in rhesus monkeys. we report here that several full-term rhesus placentas examined contain additional mpv proviral sequences in their dna. competitive molecular hybridization experiments demonstrated that some of these placentas also contain rna complementary to the entire mpv 60 to 70s rna genome. examination of internal organs of rhesus monkeys captured in the wild also reve ... | 1979 | 106962 |
| monkeypox virus (mpv). ii. uncoating and nucleic acid synthesis. | | 1972 | 4344140 |
| cellular source of interferon induced in human peripheral blood mononuclear leukocytes by mumps virus or by tumour cells persistently infected with mumps virus. | human leukocyte interferon (ifn-a) was induced in cultures of human peripheral blood mononuclear leukocytes (pbml) from seropositive healthy donor infected by mumps virus, or by a human lung carcinoma cell line (pc-10) persistently infected with mumps virus (pc-10/m-v). adherent cells (m0), non-t, non-b cells and b cells all produced ifn in response to mumps virus or pc-10/mpv cells. the non-t, non-b cells co-cultured with pc-10/mpv cells produced high-titred ifn. however, t cells did not produc ... | 1981 | 6169793 |
| the polypeptides of monkeypox virus: i. analysis of the polypeptide synthesis of mpv by sds-page and by two-dimensional electrophoresis. | | 1981 | 6261451 |
| [isolation of a murine pneumonia virus strain (mpv) and its cultivation in bhk-cells]. | | 1974 | 4446845 |
| monkeypox virus (mpv). i. propagation, purification, and further characterization. | | 1972 | 4346186 |
| humoral immunity and protection of mice challenged with homotypic or heterotypic parvovirus. | two serotypes of autonomously replicating parvoviruses infect laboratory mice. genome regions coding for the nonstructural proteins of minute virus of mice [mvm] and mouse parvovirus [mpv] are almost identical, whereas capsid-coding sequences are divergent. we addressed these questions: does humoral immunity confer protection from acute infection after challenge with homotypic or heterotypic parvovirus, and if it confers protection against acute mpv infection, does it also protect against persis ... | 1999 | 10480642 |
| carbohydrate-binding proteins of tumor lines with different growth properties. i. differences in their pattern for three clones of rat fibroblasts transformed with a myeloproliferative sarcoma virus. | three clones of myeloproliferative virus (mpv)-transformed rat fibroblasts (nrk) with different growth properties and morphology were transplanted to athymic nude mice. presence of carbohydrate-binding proteins was inferred by fluorescence microscopy using fluorescent, glycosylated markers. salt and detergent extracts of tumors from this model system were fractionated under identical conditions on different sets of sepharose columns, to which lactose, asialofetuin, melibiose, mannan and fucose h ... | 1985 | 4028124 |
| carbohydrate-binding proteins of tumor lines with different growth properties. ii. changes in their pattern in clones of transformed rat fibroblasts of differing metastatic potential. | a tumor model system of clones of myeloproliferative sarcoma virus (mpv)-transformed rat fibroblasts (nrk) with different growth properties and metastatic potential was studied. the relationship between metastatic behavior and composition of carbohydrate-binding proteins (lectins) was analyzed by affinity chromatography. the metastatic variant differs qualitatively from its parental clone in the presence of galactoside-binding proteins at apparent molecular weight of 42 kda. the alpha-glucosyl-b ... | 1986 | 3791380 |
| mason-pfizer virus rna genome: relationship to the rna of morphologically similar isolates and other oncornaviruses. | the 60-70s rna of mason-pfizer virus (mpv) was iodinated in vitro and used in both direct and competitive molecular hybridization studies. mpv proviral sequences are present at a frequency of approximately one to two copies per haploid genome in the dna of experimentally infected human cells. by nucleic acid competition hybridization, mpv rna was found to be indistinguishable from the rna of a virus (x381) isolated from a rhesus mammary gland and from rna isolated from the cytoplasm of ao cells ... | 1976 | 943573 |
| distribution of mason-pfizer virus-specific sequences in the dna of primates. | iodinated mason-pfizer virus (mpv) 60-70s rna has been used in molecular hybridization experiments to determine the distribution of mpv-specific proviral sequences in the dnas of primates. approximately 20% of the mpv genome is present as endogenous provirus in rhesus monkeys. competitive hybridization experiments showed no homology between mpv 60-70s rna and the 60-70s rnas of m7, rd-114, and the simian sarcoma virus. no mpv-specific proviral sequences were detected in the dnas of apparently no ... | 1977 | 407372 |
| mason-pfizer like virus in kidney grafted patients. | lymphocytes from a kidney grafted patient were specifically stimulated to incorporated thymidine in vitro by mitomycin c treated hela cells infected with a mason-pfizer like virus (mpv). the thermolabile mutant of vesicular stomatitis virus (vsv tl) grown in leukocytes of this patient produced pseudotypes which were neutralized by the patient's sera and boy rabbit anti-mpv sera. coculture of the patients leukocytes with sirc cells yielded virus populations with dual properties; those of mpv plus ... | 1979 | 372781 |
| poxvirus infection of the baboon (papio cynocephalus). | ten serial passages of monkeypox (mpv), vaccinia, variola, and chimpanzeepox (chimp-9) viruses were performed in baboons (papio cynocephalus) via skin scarification. comparisons of clinical and virological results indicate that mpv and vaccinia are very closely related and that variola and chimp-9 viruses are identical. these findings suggest that infections of simians with chimp-9 virus resulted from contact with variola virus, the source of which is still unknown. on the other hand, mpv in mon ... | 1976 | 191209 |
| human and primate poxviruses: i. growth characteristics of cytolytic and tumor variants. | the dual potential of poxviruses to be cytolytic and tumorgenic has been extended. yaba monkey tumor virus formed foci on monkey and human embryonic cells. cytolytic or plaque-forming virus was isolated from yaba tumor homogenates by selective sucrose centrifugation and passage through monkey or human embryonic cells. monkey pox virus (mpv) was cytolytic for monkey cells or human embryonic cells, but upon passage onto young monolayers of human carcinoma cells, hela, produced a restricted cytopat ... | 1975 | 167685 |
| the use of standard and relaxed hybridization conditions to detect two classes of sequences related to type-d retroviruses in the dnas of primates. | using standard hybridization conditions (68 degrees c and 0.4 m sodium phosphate) and assaying for rnaase-resistant rna . dna duplexes in the presence of 2 x ssc (1 x ssc is 0.15 m nacl and 0.015 m sodium citrate), sequences representing approx. 20% of the mason-pfizer virus (mpv) genome have previously been shown to be endogenous in dnas of all old world monkeys examined (drohan, w., colcher, d., schochetman, g. and schlom, j. (1977) j. virol. 23, 36--43). we now report that titration of both t ... | 1978 | 102351 |
| [effect of human interferon on viral production in a cell line chronically infected by a type d retrovirus (mason-pfizer virus)]. | human interferon specifically inhibits the viral multiplication in a human cell line infected by a type d retrovirus, the mason-pfizer virus (mpv). this inhibition is less important than for type c viruses. | 1979 | 88278 |
| [chronic infection caused by the simian mason-pfizer virus in primate cell cultures]. | cell cultures of monkey prepuce (rhfs) and african green monkey kidney (bsc-1) were infected once with simian mason-pfizer virus (mpv) and virus expression in the course of establishment of chronic infection was studied. the productive infection was characterized by changes in the cell metabolism (dna synthesis increased 2-3-fold as early as the "zero" passage), the appearance of gs-antigen, formation of virions of type d and high activity of rna-dependent dna-polymerase. multinuclear giant cell ... | 1978 | 78587 |
| squirrel monkey retrovirus: an endogenous virus of a new world primate. | squirrel monkey retrovirus (smrv) was isolated by cocultivation of squirrel monkey lung cells with canine cells. 3h-labeled 60-70s smrv rna, isolated from virus grown in canine cells, hybridized to the same extent and to the same cot1/2 value to the dna of all tissues of all squirrel monkeys tested; cot1/2 values show that smrv proviral sequences are present in the low repetitive range. no smrv proviral sequences were detected in tissues from a variety of other new world monkeys, old world monke ... | 1977 | 69724 |
| identification of a novel lymantria dispar nucleopolyhedrovirus mutant that exhibits abnormal polyhedron formation and virion occlusion. | in previous studies on the formation of lymantria dispar nuclear polyhedrosis virus (ldmnpv) few polyhedra (fp) mutants, several polyhedron formation mutants (pfm) were identified that appeared to be unique. these viral mutants are being characterized to investigate the processes of polyhedron formation and virion occlusion. ldmnpv isolate pfm-1 is one of these mutants, and is described in this report. genetic techniques were used to determine if isolate pfm-1 contained a mutation in the polyhed ... | 1998 | 9647698 |
| nucleotide and predicted amino acid sequence-based analysis of the avian metapneumovirus type c cell attachment glycoprotein gene: phylogenetic analysis and molecular epidemiology of u.s. pneumoviruses. | a serologically distinct avian metapneumovirus (ampv) was isolated in the united states after an outbreak of turkey rhinotracheitis (trt) in february 1997. the newly recognized u.s. virus was subsequently demonstrated to be genetically distinct from european subtypes and was designated ampv serotype c (ampv/c). we have determined the nucleotide sequence of the gene encoding the cell attachment glycoprotein (g) of ampv/c (colorado strain and three minnesota isolates) and predicted amino acid sequ ... | 2003 | 12682171 |
| mouse parvovirus infection potentiates allogeneic skin graft rejection and induces syngeneic graft rejection. | the recently identified autonomous mouse parvovirus designated mouse parvovirus-1 (mpv-1) persists in adult balb/c mice for at least 9 weeks, infects lymphoid tissues, interferes with the ability of cloned t cells to proliferate, and exhibits immunomodulatory properties. as a consequence of these findings, the present studies were undertaken to characterize further the inmunomodulatory effects of mpv-1 on t cell-mediated immune responses in vivo and in vitro. | 1998 | 9645799 |
| ineffective platelet production in thrombocytopenic human immunodeficiency virus-infected patients. | thrombocytopenia has been characterized in six patients infected with human immunodeficiency virus (hiv) with respect to the delivery of viable platelets into the peripheral circulation (peripheral platelet mass turnover), marrow megakaryocyte mass (product of megakaryocyte number and volume), megakaryocyte progenitor cells, circulating levels of endogenous thrombopoietin (tpo) and platelet tpo receptor number, and serum antiplatelet glycoprotein (gp) iiia49-66 antibody (gpiiia49-66ab), an antib ... | 1998 | 9558379 |
| efficacy of three microbiological monitoring methods in a ventilated cage rack. | the use of individually ventilated caging (ivc) to house mice presents new challenges for effective microbiological monitoring. methods that exploit the characteristics of ivc have been developed, but to the authors' knowledge, their efficacy has not been systematically investigated. air exhausted from the ivc rack can be monitored, using sentinels housed in cages that receive rack exhaust air as their supply air, or using filters placed on the exhaust air port. to aid laboratory animal personne ... | 2004 | 15357318 |
| human monkeypox -- kasai oriental, democratic republic of congo, february 1996-october 1997. | human monkeypox is a severe smallpox-like illness caused by monkeypox virus (mpv); monkeypox occurs in sporadic outbreaks, and infection is enzootic among squirrels and monkeys in the rainforests of western and central africa. in 1996, cases of monkeypox were reported from villages in the katako-kombe health zone, kasai oriental, zaire (i.e., democratic republic of congo). the world health organization (who), in collaboration with cdc, investigated this outbreak and identified 92 suspected cases ... | 1997 | 9408046 |
| candidate multi-peptide-vaccine against classical swine fever virus induced potent immunity with serological marker. | our previous study proposed a protective multi-peptide-vaccine (mpv) with freund's adjuvant against classical swine fever virus (csfv). in this study, another candidate mpv, using aluminum adjuvant, was further examined. all immunized pigs kept healthy during the experimental period, while the control group rapidly showed clinical symptoms and died. moreover, anti-sera from mpv-immunized pigs could interact with peptides involved in the mpv, in contrast to anti-sera from non-immunized or infecte ... | 2005 | 15882522 |
| genetic diversity in marine algal virus communities as revealed by sequence analysis of dna polymerase genes. | algal-virus-specific pcr primers were used to amplify dna polymerase gene (pol) fragments (683 to 689 bp) from the virus-sized fraction (0.02 to 0.2 microns) concentrated from inshore and offshore water samples collected from the gulf of mexico. algal-virus-like dna pol genes were detected in five samples collected from the surface and deep chlorophyll maximum. pcr products from an offshore station were cloned, and the genetic diversity of 33 fragments was examined by restriction fragment length ... | 1996 | 8702280 |
| detection and quantification of human metapneumovirus in pediatric specimens by real-time rt-pcr. | human metapneumovirus (h mpv), a recently identified virus, causes respiratory illness in children. | 2005 | 16036180 |
| molecular characterization of newly recognized rodent parvoviruses. | several autonomous rodent parvoviruses distinct from the prototypic rodent parvoviruses have been isolated. these include variants of a mouse parvovirus (mpv), a hamster isolate designated hamster parvovirus (hapv), and a variant strain of minute virus of mice (mvm) designated mvm-cutter or mvm(c). in this study, the dna sequence of the coding regions of the viral genome and the predicted protein sequences for each of these new isolates were determined and compared to the immunosuppressive and p ... | 1996 | 8609486 |
| molecular characterization of a newly recognized mouse parvovirus. | mouse parvovirus (mpv), formerly known as orphan parvovirus, is a newly recognized rodent parvovirus distinct from both serotypes of minute virus of mice (mvm). restriction analysis of the mpv genome indicated that many restriction sites in the capsid region were different from those of mvm, but most sites in the nonstructural (ns) region of the genome were conserved. mpv resembled mvm in genome size, replication intermediates, and ns proteins. replication intermediates in infected cells were th ... | 1994 | 8083985 |
| antemortem detection of mouse parvovirus and mice minute virus by polymerase chain reaction (pcr) of faecal samples. | parvoviruses remain one of the most common viral infections seen in laboratory mouse colonies. the purpose of this study was to develop an antemortem polymerase chain reaction (pcr) assay to detect mice infected with mouse parvovirus-1 (mpv) and mice minute virus (mmv) using faecal samples. the mmv pcr assay consistently detected as few as 100 plasmid copies of mmv in faecal samples, while the mpv pcr assay detected as few as 10 plasmid copies of mpv. faecal pellets from infected mice held at ro ... | 2006 | 16600074 |
| amplification of dna polymerase gene fragments from viruses infecting microalgae. | nested pcr with three highly degenerate primers was used for amplification and identification of dna polymerase (pol) genes from viruses which infect three genera of microalgae. group-specific primers (avs1 and avs2) were designed on the basis of inferred amino acid sequences unique to the dna pol genes of viruses (pbcv-1 and ny-2a) that infect an endosymbiotic chlorella-like alga (chlorophyceae) and a virus (mpv-sp1) which infects the photosynthetic flagellate micromonas pusilla (prasinophyceae ... | 1995 | 7747950 |
| immunological characterization of type-d retrovirus isolates from human cells. | competition radioimmunoassays for the 27,000 molecular weight(p27) major internal protein and the low-molecular-weight 10,000-12,000 (p10-12) polypeptides of the primate type-d retroviruses, mason-pfizer virus(mpv) and langur virus (lv), were established. type-d isolates from various primate and human tumor cells were examined in both these assays. there were no detectable immunological differences between mpv and two type-d retroviruses previously reported to be isolated from human cell lines ( ... | 1980 | 7251333 |
| the molecular biology of yaba tumour pox virus: analysis of lipids, proteins and dna. | cytopathological studies have shown that yaba tumour pox virus (yaba virus) infection leads to the accumulation of large lipid vacuoles. the rate of accumulation of these vacuoles increased as the infection proceeded. these lipid vacuoles were not seen in control cells or in cells infected with monkeypox virus (mpv) but were seen during yaba virus infection in the presence of cytosine arabinofuranoside (100 microgram/ml). yaba virus also failed to inhibit host protein synthesis as infection proc ... | 1982 | 6292349 |
| monoclonal antibodies to a monkeypox virus polypeptide determinant. | three monkeypox virus (mpv) antibody-secreting murine monoclones were characterized as being of the immunoglobulin g1 isotype, gave a 4+ reaction in the indirect fluorescent-antibody test, gave a positive reaction in the enzyme immunoassay, and did not neutralize mpv. these monoclonal antibodies were determined by the sodium dodecyl sulfate-polyacrylamide gel electrophoresis transblot method to react to a 15,500-molecular-weight mpv polypeptide. this reactivity could not be removed by adsorption ... | 1984 | 6207310 |
| enhancement of cytotoxicity of human peripheral blood lymphocytes by interferon. | human lung carcinoma cells persistently infected with mumps virus (pc-10/mpv) were lysed with human peripheral blood mononuclear leukocytes (pbml) obtained from seropositive donors who had anti-mumps virus-neutralizing antibody in their sera. this cellular cytotoxicity was due not to the cytotoxic t lymphocytes but mainly to the non-t, non-b cells, possibly related to natural killer (nk) cells. moreover, it was concerned not with antibody against mumps virus antigens but with alpha-interferon (i ... | 1981 | 6169978 |
| specific detection of monkeypox virus by polymerase chain reaction. | the open reading frame coding for the a-type inclusion body protein (ati) of monkeypox virus (mpv) was identified and sequenced for two strains. nucleotide sequence comparison revealed 72-95.3% homology with the reported open reading frame sequences of the atis of other orthopoxvirus species, such as variola, vaccinia, cowpox, ectromelia, and camelpox viruses. each mpv strain contained an 8-bp deletion, which caused a frameshift that introduced a premature stop in the open reading frame at base ... | 1998 | 9779620 |
| effect of mouse strain and age on detection of mouse parvovirus 1 by use of serologic testing and polymerase chain reaction analysis. | detection of mouse parvovirus 1 (mpv) depends on use of serologic and polymerase chain reaction (pcr) assays. these assays were evaluated for their ability to detect virus-specific antibodies or viral dna in multiple strains and ages of mice inoculated with mpv. | 2000 | 11099132 |
| the immune response to vaccinia virus is significantly reduced after scarification with tk- recombinants as compared to wild-type virus. | although it is unlikely that large-scale vaccination against smallpox will ever be required again, it is conceivable that the need may arise to vaccinate against a human orthopoxvirus infection. a possible example could be the emergence of monkey poxvirus (mpv) as a significant human disease in africa. vaccinia virus (vv) recombinants, genetically modified to carry the immunogenic proteins of other pathogenic organisms, have potential use as vaccines against other diseases present in this region ... | 2000 | 11155357 |
| the prevalence of viral antibodies during a large population fluctuation of house mice in australia. | we studied the seroprevalence of three viruses (mouse cytomegalovirus (mcmv), minute virus of mice (mvm), and mouse parvovirus (mpv)) in house mice (mus domesticus) in 1995 7. in the first year average mouse density was less than 1 mouse/ha. from november 1995 to may 1996 the population increased at an average rate of 7% per week, a doubling time of about 10 weeks. from august 1996 to may 1997 the population increased at an average rate of 10% per week, a doubling time of about 7.5 weeks. from a ... | 2000 | 11218223 |
| species-specific differences in the structure of orthopoxvirus complement-binding protein. | vaccinia virus complement-binding protein (vcp) is secreted from the cells infected with the virus and controls the complement activation reactions. this protein contains four short consensus repeats (scr), typical of the protein family of complement activation regulators. organization of the vcp genes/proteins of orthopoxviruses-monkeypox (mpv), variola, cowpox and vaccinia viruses-and their cellular homologues (daf and c4bp) were studied comparatively. for this purpose, vcp genes of three mpv ... | 2001 | 11682123 |
| human monkeypox and smallpox viruses: genomic comparison. | monkeypox virus (mpv) causes a human disease which resembles smallpox but with a lower person-to-person transmission rate. to determine the genetic relationship between the orthopoxviruses causing these two diseases, we sequenced the 197-kb genome of mpv isolated from a patient during a large human monkeypox outbreak in zaire in 1996. the nucleotide sequence within the central region of the mpv genome, which encodes essential enzymes and structural proteins, was 96.3% identical with that of vari ... | 2001 | 11734207 |
| detection of rodent parvoviruses by use of fluorogenic nuclease polymerase chain reaction assays. | polymerase chain reaction (pcr) assays have proven useful for detection of rodent parvoviruses in animals and contaminated biological materials. fluorogenic nuclease pcr assays combine pcr with an internal fluorogenic hybridization probe, eliminating post-pcr processing and potentially enhancing specificity. consequently, three fluorogenic nuclease pcr assays were developed, one that detects all rodent parvoviruses, one that specifically detects minute virus of mice (mvm), and one that specifica ... | 2001 | 11924790 |
| species-specific differences in organization of orthopoxvirus kelch-like proteins. | organization of orthopoxvirus proteins of the kelch superfamily and their genes were analyzed and compared. complete genomic sequences of variola (var), monkeypox (mpv), vaccinia (vac), and species-specific regions of cowpox (cpv) viruses were used in the work. despite the multiplicity of kelch-like proteins in orthopoxviruses, their function is still vague. it has been discovered that the genes of orthopoxvirus kelch-like proteins are localized only to the terminal variable regions of the genom ... | 2002 | 12018707 |
| validation of an enzyme-linked immunosorbent assay for detection of mouse parvovirus infection in laboratory mice. | parvoviruses are among the most prevalent infectious agents in mouse colonies. infection in laboratory mice is confirmed by detection of serum antibodies to these agents, and most diagnostic tests cannot distinguish serogroup of the infecting agent. the principal objective of the research reported here was to develop and validate a sensitive, serogroup-specific diagnostic test that will distinguish between mouse parvovirus (mpv) and minute virus of mice (mvm) infection. | 2002 | 12022396 |
| analysis of the monkeypox virus genome. | monkeypox virus (mpv) belongs to the orthopoxvirus genus of the family poxviridae, is endemic in parts of africa, and causes a human disease that resembles smallpox. the 196,858-bp mpv genome was analyzed with regard to structural features and open reading frames. each end of the genome contains an identical but oppositely oriented 6379-bp terminal inverted repetition, which similar to that of other orthopoxviruses, includes a putative telomere resolution sequence and short tandem repeats. compu ... | 2002 | 12083817 |
| novel engineered hiv-1 east african clade-a gp160 plasmid construct induces strong humoral and cell-mediated immune responses in vivo. | hiv-1 sequences are highly diverse due to the inaccuracy of the viral reverse transcriptase. this diversity has been studied and used to categorize hiv isolates into subtypes or clades, which are geographically distinct. to develop effective vaccines against hiv-1, immunogens representing different subtypes may be important for induction of cross-protective immunity, but little data exist describing and comparing the immunogenicity induced by different subtype-based vaccines. this issue is furth ... | 2003 | 14517067 |
| rapid detection and differentiation of human pathogenic orthopox viruses by a fluorescence resonance energy transfer real-time pcr assay. | the orthopox viruses that are pathogenic for humans include variola major virus (var), monkeypox virus (mpv), cowpox virus (cpv), and to a lesser extent, camelpox virus (cml) and vaccinia virus (vac). pcr is a powerful tool to detect and differentiate orthopox viruses, and real-time pcr has the further advantages of rapid turnaround time, low risk of contamination, capability of strain differentiation, and use of multiplexed probes. | 2004 | 14962998 |
| human metapneumovirus: a new pathogen in children and adults. | in 2001, human metapneumovirus (hmpv) was discovered in young children with respiratory tract infection of unknown origin. in the two years since its discovery the dinical characteristics of this new virus have been darified. in children, especially those younger than one year of age, hmpv is responsible for 5 to 10% of respiratory tract infections requiring hospitalisation; its clinical course is somewhat milder, but otherwise indistinguishable from respiratory syncytial virus (rsv) infection. ... | 2004 | 15460495 |
| population dynamics and rates of molecular evolution of a recently emerged paramyxovirus, avian metapneumovirus subtype c. | we report the existence of two distinct sublineages of avian metapneumovirus (mpv) subtype c, a virus which has caused serious economic loss in commercial turkey farms in the united states. this subtype is closely related to human mpv, infects multiple avian species, and is globally distributed. the evolutionary rates of this virus are estimated to be 1.3 x 10(-3) to 7 x 10(-3) substitutions per site per year, and coalescent estimates place its emergence between 1991 and 1996. the four genes exa ... | 2009 | 19052092 |
| diagnosis of human metapneumovirus and rhinovirus in patients with respiratory tract infections by an internally controlled multiplex real-time rna pcr. | adequate laboratory diagnosis of human rhinoviruses (hrv) and human metapneumoviruses (h mpv) requires molecular methods as viral culture lacks sensitivity. however, setting up individual pcrs for all respiratory viruses is not practical so preferentially multiplex pcrs are used. | 2005 | 15994117 |
| activity and regulation of alpha interferon in respiratory syncytial virus and human metapneumovirus experimental infections. | respiratory syncytial virus (rsv) and human metapneumovirus (hmpv) cause a similar spectrum of respiratory infections in humans. classified within the paramyxoviridae family, pneumovirinae subfamily, rsv and hmpv present a significant degree of divergence in genome constellation, organization, and protein sequences. rsv has been reported to be a poor inducer of alpha/beta interferons (ifn-alpha/beta) and partially resistant to its antiviral activity. the nature of the innate immune response to h ... | 2005 | 16051812 |
| genetic diversity of algal viruses which lyse the photosynthetic picoflagellate micromonas pusilla (prasinophyceae). | the genetic similarity among eight clones of micromonas pusilla virus (mpv) isolated from five geographic locations was measured by dna hybridization. our objective was to explore the existence of genetically distinct populations of mpv by comparing the similarity among mpvs isolated from a single water sample to the similarity among viruses isolated from geographically distant locations. the highest and lowest similarities we observed were 70% (plusmn) 1.1% (mean (plusmn) standard error [se], n ... | 1995 | 16535105 |
| development of elisa using recombinant antigens for specific detection of mouse parvovirus infection. | nucleotide sequences of mouse parvovirus (mpv) isolate, named mpv/ut, and mouse minute virus (mmv) were analyzed and used for expressing recombinant proteins in e. coli. elisa tests using recombinant major capsid protein (rvp2) and recombinant major non-structural protein (rns1) as antigens were developed and their performance in serologic detection of rodent parvovirus infection was assessed. mpv-rvp2 and mmv-rvp2 elisas reacted specifically with anti-mpv and anti-mmv mouse sera, respectively. ... | 2006 | 16651694 |
| human metapneumovirus elicits weak ifn-gamma memory responses compared with respiratory syncytial virus. | human metapneumovirus (mpv) is a recently discovered pathogen that causes repeated lower respiratory tract infections beginning in infancy. the prevalence, nature and control of human regulatory responses to mpv are unknown. in this study, we develop and optimize systems to evaluate mpv-driven cytokine responses. using primary culture of human pbmc from previously exposed adults, mpv-stimulated responses were directly compared with those elicited by genetically and clinically similar respiratory ... | 2006 | 16670291 |
| identification of novel murine parvovirus strains by epidemiological analysis of naturally infected mice. | random-source dna samples obtained from naturally infected laboratory mice (n=381) were evaluated by pcr and rflp analysis to determine the prevalence of murine parvovirus strains circulating in contemporary laboratory mouse colonies. mouse parvovirus (mpv) was detected in 77% of samples, minute virus of mice (mvm) was detected in 16% of samples and both mvm and mpv were detected in 7% of samples. mvmm, a strain recently isolated from clinically ill nod-mu chain knockout mice, was detected in 91 ... | 2006 | 16690918 |
| microbiological monitoring of laboratory mice and biocontainment in individually ventilated cages: a field study. | over recent years, the use of individually ventilated cage (ivc) rack systems in laboratory rodent facilities has increased. since every cage in an ivc rack may be assumed to be a separate microbiological unit, comprehensive microbiological monitoring of animals kept in ivcs has become a challenging task, which may be addressed by the appropriate use of sentinel mice. traditionally, these sentinels have been exposed to soiled bedding but more recently, the concept of exposure to exhaust air has ... | 2006 | 16803642 |
| respiratory virus pneumonia after hematopoietic cell transplantation (hct): associations between viral load in bronchoalveolar lavage samples, viral rna detection in serum samples, and clinical outcomes of hct. | few data exist on respiratory virus quantitation in lower respiratory samples and detection in serum from hematopoietic cell transplant (hct) recipients with respiratory virus-associated pneumonia. | 2010 | 20350162 |
| increased mean platelet volume in chronic hepatitis b patients with inactive disease. | hepatitis b virus (hbv) infection is a major public health problem worldwide, responsible for considerable morbidity and mortality from chronic liver disease. conflicting findings on the possible association between hbv surface antigen (hbsag) positivity, indicating inactive hbsag carrier status, and atherosclerosis have been reported. platelet activation and aggregation are central processes in the pathophysiology of atherosclerosis. mean platelet volume, a determinant of platelet activation, i ... | 2010 | 20357720 |
| comparison of real-time pcr assays with fluorescent-antibody assays for diagnosis of respiratory virus infections in children. | conventional fluorescent-antibody (fa) methods were compared to real-time pcr assays for detection of respiratory syncytial virus (rsv), influenza virus type a (flua), parainfluenza virus types 1, 2, and 3 (piv1, piv2, and piv3), human metapneumovirus (mpv), and adenovirus (adv) in 1,138 specimens from children with respiratory illnesses collected over a 1-year period. at least one virus was detected in 436 (38.3%) specimens by fa and in 608 (53.4%) specimens by pcr (p<0.001). specimen quality w ... | 2006 | 16825353 |
| detection of mouse parvovirus in mus musculus gametes, embryos, and ovarian tissues by polymerase chain reaction assay. | we used primary and nested polymerase chain reaction (pcr) assays to determine the presence of mouse parvovirus (mpv) in mouse sperm, oocytes, preimplantation embryos, and ovarian tissues collected from mpv-infected mice. the primary pcr assay detected mpv in 56% of the sperm samples. mpv was not eliminated by passing sperm samples through a percoll gradient. after percoll treatment, mpv was still present in 50% of the samples according to primary pcr assay. oocyte samples that did not undergo e ... | 2007 | 17348291 |
| reliability of soiled bedding transfer for detection of mouse parvovirus and mouse hepatitis virus. | serologic monitoring of sentinel mice exposed to soiled bedding is a common method of detecting viral infections in mice. because bedding transfer protocols vary, the sensitivity of this method has not been documented sufficiently. we examined the reliability of bedding transfer during various stages of infection with mouse parvovirus (mpv) and mouse hepatitis virus (mhv). most mice exposed to bedding contaminated with mpv 0, 3, or 7 d previously seroconverted, whereas only mice exposed to beddi ... | 2007 | 17348296 |
| respiratory virus infection among hematopoietic cell transplant recipients: evidence for asymptomatic parainfluenza virus infection. | the incidence of respiratory virus infection after hematopoietic cell transplantation (hct) has probably been underestimated with conventional testing methods in symptomatic patients. this prospective study assessed viral infection episodes by testing weekly respiratory samples collected from hct recipients, with and without symptoms reported by questionnaire, for 100 days after hct. samples were tested by culture and direct fluorescent antibody testing for respiratory syncytial virus (rsv), par ... | 2007 | 17502457 |
| resource dynamics during infection of micromonas pusilla by virus mpv-sp1. | viruses infecting marine phytoplankton drive phytoplankton diversity, terminate blooms and shuttle genetic material. assessments of the scale of viral impacts on trophic networks are, however, speculative. we investigated fluxes of dna between host and virus during infection of the prasinophyte alga micromonas pusilla by phycodnavirus mpv sp1. under a light-dark regimen, viral genomes accumulated to a transient peak within 24 h, at the expense of both host dna synthesis and nuclear dna. viral ge ... | 2007 | 17922756 |
| multiplex real-time pcr for detection of respiratory tract infections. | broad diagnostics of respiratory infection by molecular assays has not yet won acceptance due to technical difficulties and high costs. | 2008 | 18093871 |
| transmission probabilities of mouse parvovirus 1 to sentinel mice chronically exposed to serial dilutions of contaminated bedding. | intermittent serodetection of mouse parvovirus (mpv) infections in animal facilities occurs frequently when soiled bedding sentinel mouse monitoring systems are used. we evaluated induction of seroconversion in naïve single-caged weanling icr mice (n = 10 per group) maintained on 5-fold serially diluted contaminated bedding obtained from scid mice persistently shedding mpv1e. soiled bedding from the infected scid mice was collected, diluted, and redistributed weekly to cages housing icr mice to ... | 2008 | 18524171 |
| management strategies for controlling endemic and seasonal mouse parvovirus infection in a barrier facility. | despite improved diagnostic and rederivation capabilities, research facilities still struggle to manage parvovirus infections (e.g., mouse parvovirus (mpv) and minute virus of mice) in mouse colonies. multi-faceted approaches are needed to prevent adventitious organisms such as mpv from breaching a barrier facility. in this article, the authors document recent changes to the salk institute's animal care program that were intended to help manage mouse parvovirus in the barrier facility. specifica ... | 2011 | 21508953 |
| monkeypox virus viral chemokine inhibitor (mpv vcci), a potent inhibitor of rhesus macrophage inflammatory protein-1. | monkeypox virus (mpv) is an orthopoxvirus with considerable homology to variola major, the etiologic agent of smallpox. although smallpox was eradicated in 1976, the outbreak of mpv in the u.s. highlights the health hazards associated with zoonotic infections. like other orthopoxviruses, mpv encodes a secreted chemokine binding protein, vcci that is abundantly expressed and secreted from mpv infected cells. emsa data shows vcci efficiently binds rhesus mip-1alpha (rhmip-1alpha) at near one to on ... | 2008 | 18639466 |
| development and application of a reverse transcriptase polymerase chain reaction to detect chinese isolates of duck hepatitis virus type 1. | we developed a reverse transcriptase polymerase chain reaction (rt-pcr) method for the detection of duck hepatitis virus type 1 (dhv-1) in the tissues of infected and clinically affected ducks and in chick and duck embryos. we found the assay to be effective in detecting the virus in china, where it is being used in studies on the epidemiology of the disease. we applied this simple and rapid diagnostic method to the detection of dhv isolates grown in chick and duck embryos and in tissues obtaine ... | 2009 | 18706944 |
| embryo transfer rederivation of c.b-17/icr-prkdc(scid) mice experimentally infected with mouse parvovirus 1. | we determined whether embryos derived from c.b-17/icr-prkdc(scid) (scid) mice infected with mouse parvovirus (mpv) 1b and mated to mpv-naive b6c3f1 mice would transmit virus to naive recipient female mice and rederived progeny. viral dna was detected by quantitative pcr (qpcr) in lymphoid tissues, gonad, sperm, and feces of all mpv1b-inoculated scid mice. viral dna was detected in 1 of 16 aliquots of embryos from infected male scid mice and in 12 of 18 aliquots of embryos from infected female sc ... | 2008 | 18724777 |
| [orthopoxvirus genes for kelch-like proteins. i. analysis of species specific differences by gene structure and organization]. | genes and proteins of the kelch superfamily were structurally analyzed in the smallpox (spv), monkeypox (mpv), cowpox (cpv), and vaccinia (vv) viruses. genes potentially coding for the kelch-like proteins were found only in the variable terminal regions of the orthopoxvirus genome. the set and sizes of their protein products varied with species. all genes of the superfamily proved to be disrupted by mutations in spv, which is highly pathogenic for its only host, man. the largest set of kelch-lik ... | 2002 | 12173463 |
| serodiagnosis of mice minute virus and mouse parvovirus infections in mice by enzyme-linked immunosorbent assay with baculovirus-expressed recombinant vp2 proteins. | mice minute virus (mmv) and mouse parvovirus (mpv) type 1 are the two parvoviruses known to naturally infect laboratory mice and are among the most prevalent infectious agents found in contemporary laboratory mouse colonies. serologic assays are commonly used to diagnose mmv and mpv infections in laboratory mice; however, highly accurate, high-throughput serologic assays for the detection of mmv- and mpv-infected mice are needed. to this end, the major capsid viral protein (vp2) genes of mmv and ... | 2002 | 12204954 |
| candidate peptide vaccine induced protection against classical swine fever virus. | former investigations demonstrated that the envelope glycoprotein e2 could protect pigs from classical swine fever virus (csfv). based on these findings, we prepared synthetic peptide vaccine using e2 n-terminal antigenic units b/c and hoped to induce protective activity against lethal challenge of virulent csfv strain shimen. five overlapped peptides sequence-covering amino acids 693-777 on e2 of shimen were synthesized and then conjugated with bovine serum albumin (bsa), respectively. in the v ... | 2002 | 12450690 |
| quantitative characterization of virus-like particles by asymmetrical flow field flow fractionation, electrospray differential mobility analysis, and transmission electron microscopy. | here we characterize virus-like particles (vlps) by three very distinct, orthogonal, and quantitative techniques: electrospray differential mobility analysis (es-dma), asymmetric flow field-flow fractionation with multi-angle light scattering detection (affff-mals) and transmission electron microscopy (tem). vlps are biomolecular particles assembled from viral proteins with applications ranging from synthetic vaccines to vectors for delivery of gene and drug therapies. vlps may have polydisperse ... | 2009 | 18958863 |
| encapsulation of dna and non-viral protein changes the structure of murine polyomavirus virus-like particles. | asymmetrical-flow field flow fractionation with multiple-angle light scattering (affff-mals) was, for the first time, used to characterize the size of murine polyomavirus virus-like particles (mpv vlps) packaged with either insect cell genomic dna or non-viral protein. encapsidation of both genomic dna and non-viral protein were found to cause a contraction in vlp radii of gyration by approximately 1 nm. non-viral protein packaged into vlps consisted of a series of glutathione-s-transferase, his ... | 2008 | 18979257 |
| comparison of conventional and molecular detection of respiratory viruses in hematopoietic cell transplant recipients. | sensitive detection of respiratory viruses is important for early diagnosis of infection in patients following hematopoietic cell transplantation (hct). to evaluate the relative sensitivity of respiratory virus detection in specimens from hct recipients, we compared the results of conventional and quantitative molecular methods. | 2009 | 19453994 |
| development and application of a reverse transcriptase polymerase chain reaction to detect chinese isolates of duck hepatitis virus type 1. | we developed a reverse transcriptase polymerase chain reaction (rt-pcr) method for the detection of duck hepatitis virus type 1 (dhv-1) in the tissues of infected and clinically affected ducks and in chick and duck embryos. we found the assay to be effective in detecting the virus in china, where it is being used in studies on the epidemiology of the disease. we applied this simple and rapid diagnostic method to the detection of dhv isolates grown in chick and duck embryos and in tissues obtaine ... | 2009 | 19475729 |
| genotypes and clinical data of respiratory syncytial virus and metapneumovirus in brazilian infants: a new perspective. | the aim of this study was to determine if there was a correlation between respiratory syncytial virus (rsv) and metapneumovirus (mpv) genotypes and clinical data of brazilian infants hospitalized for acute lower respiratory infection. the viruses in the patients' nasopharyngeal secretions were studied using the polymerase chain reaction and phylogenetic analysis. the study assessed 144 infants; 31.9% were rsv positive and 5.6% were mpv positive. statistical analysis was performed using the chi-s ... | 2009 | 19578627 |
| effects of ultraviolet radiation on marine virus-phytoplankton interactions. | abstract ambient ultraviolet radiation (uvr) is harmful to many biological systems and increased uvr, due to a reduced ozone layer, may have many unforeseen consequences. viruses are the most abundant biological particles in the sea and are thought to play an important role in the structure and functioning of aquatic ecosystems. although an increasing number of studies have been published during the last 15 years, aquatic viral ecology is still in its infancy and little is known about the effect ... | 2003 | 19719609 |
| expansion, reexpansion, and recall-like expansion of vgamma2vdelta2 t cells in smallpox vaccination and monkeypox virus infection. | little is known about the in vivo kinetics of t-cell responses in smallpox/monkeypox. we showed that macaque vgamma2vdelta2 t cells underwent 3-week-long expansion after smallpox vaccine immunization and displayed simple reexpansion in association with sterile anti-monkeypox virus (anti-mpv) immunity after mpv challenge. virus-activated vgamma2vdelta2 t cells exhibited gamma interferon-producing effector function after phosphoantigen stimulation. surprisingly, like alphabeta t cells, suboptimall ... | 2009 | 19740988 |
| inhibition of monkeypox virus replication by rna interference. | the orthopoxvirus genus of poxviridae family is comprised of several human pathogens, including cowpox (cpxv), vaccinia (vacv), monkeypox (mpv) and variola (varv) viruses. species of this virus genus cause human diseases with various severities and outcome ranging from mild conditions to death in fulminating cases. currently, vaccination is the only protective measure against infection with these viruses and no licensed antiviral drug therapy is available. in this study, we investigated the pote ... | 2009 | 19889227 |
| vaccination of grouper broodfish (epinephelus tukula) reduces the risk of vertical transmission by nervous necrosis virus. | nervous necrosis virus (nnv) has caused mass mortality in many species of cultured fish at larval stage. strong evidence of vertical transmission of nnv has been reported in the carrier broodstock of striped jack and sea bass. an effective immunization program was developed and monitored in adult groupers (epinephelus coioides) with average body weight of 1.35kg. the highest neutralizing antibody titers were found in the fish intramuscularly injected with adjuvanted nnv vaccine at 10(9)tcid(50)k ... | 2010 | 19954763 |
| experimental infection of mice with hamster parvovirus: evidence for interspecies transmission of mouse parvovirus 3. | hamster parvovirus (hapv) was isolated 2 decades ago from hamsters with clinical signs similar to those induced in hamsters experimentally infected with other rodent parvoviruses. genetically, hapv is most closely related to mouse parvovirus (mpv), which induces subclinical infection in mice. a novel mpv strain, mpv3, was detected recently in naturally infected mice, and genomic sequence analysis indicates that mpv3 is almost identical to hapv. the goal of the present studies was to examine the ... | 2010 | 20412687 |
| comparative whole genome sequence analysis of wild-type and cidofovir-resistant monkeypoxvirus. | we performed whole genome sequencing of a cidofovir {[(s)-1-(3-hydroxy-2-phosphonylmethoxy-propyl) cytosine] [hpmpc]}-resistant (cdv-r) strain of monkeypoxvirus (mpv). whole-genome comparison with the wild-type (wt) strain revealed 55 single-nucleotide polymorphisms (snps) and one tandem-repeat contraction. over one-third of all identified snps were located within genes comprising the poxvirus replication complex, including the dna polymerase, rna polymerase, mrna capping methyltransferase, dna ... | 2010 | 20509894 |
| epidemiology of viral respiratory tract infections in a prospective cohort of infants and toddlers attending daycare. | the epidemiology of respiratory tract infections (rtis) in a daycare cohort has not been explored using molecular techniques. | 2010 | 20650679 |
| gene expression profiling of monkeypox virus-infected cells reveals novel interfaces for host-virus interactions. | monkeypox virus (mpv) is a zoonotic orthopoxvirus and a potential biothreat agent that causes human disease with varying morbidity and mortality. members of the orthopoxvirus genus have been shown to suppress antiviral cell defenses, exploit host cell machinery, and delay infection-induced cell death. however, a comprehensive study of all host genes and virus-targeted host networks during infection is lacking. to better understand viral strategies adopted in manipulating routine host biology on ... | 2010 | 20667104 |
| development and evaluation of an immunochromatographic strip test based on the recombinant ul51 protein for detecting antibody against duck enteritis virus. | duck enteritis virus (dev) infection causes substantial economic losses to the worldwide duck-producing areas. the monitoring of dev-specific antibodies is a key to evaluate the effect of dev vaccine and develop rational immunization programs. thus, in this study, an immunochromatographic strip (ics) test was developed for detecting dev serum antibodies. | 2010 | 20946624 |
| stunned silence: gene expression programs in human cells infected with monkeypox or vaccinia virus. | poxviruses use an arsenal of molecular weapons to evade detection and disarm host immune responses. we used dna microarrays to investigate the gene expression responses to infection by monkeypox virus (mpv), an emerging human pathogen, and vaccinia virus (vac), a widely used model and vaccine organism, in primary human macrophages, primary human fibroblasts and hela cells. even as the overwhelmingly infected cells approached their demise, with extensive cytopathic changes, their gene expression ... | 2011 | 21267444 |
| genomic analysis of a pneumovirus isolated from dogs with acute respiratory disease. | a previously unrecognized virus belonging to the subfamily pneumovirinae and most closely related to murine pneumovirus (mpv) was identified in domestic dogs in 2 related animal shelters. additional diagnostic testing yielded 3 new viral isolates and identified 6 additional pcr positive dogs from other usa locations indicating that its distribution is not geographically limited. nucleotide sequences encompassing 9 of the 10 genes were compared to the only 2 available mpv strains, 15 and j3666. s ... | 2011 | 21324612 |
| effects of the polymorphisms of mx1, bat2 and cxcl12 genes on immunological traits in pigs. | it is necessary that genetic markers or biomarkers can be used to predict resistance towards a wide range of infectious diseases. in the present study, we estimated the potential markers and measured their relationship with heritabilities of a wide range of immune traits. polymorphisms in exon 13 of mx1, intron 25 of bat2 and intron 3 of cxcl12 were identified by sequencing, and the genotypes were analyzed by pcr-rflp in a resource population composed of 352 pure breed landrace piglets at days 0 ... | 2011 | 21667240 |
| coping with parvovirus infections in mice: health surveillance and control. | parvoviruses of mice, minute virus of mice (mvm) and mouse parvovirus (mpv), are challenging pathogens to eradicate from laboratory animal facilities. due to the impediment on rodent-based research, recent studies have focused on the assessment of re-derivation techniques and parvoviral potential to induce persistent infections. summarizing recent data, this review gives an overview on studies associated with parvoviral impact on research, diagnostic methods, parvoviral persistence and re-deriva ... | 2011 | 22037057 |
| identification of potential human respiratory syncytial virus and metapneumovirus t cell epitopes using computational prediction and mhc binding assays. | human respiratory syncytial virus (rsv) and human metapneumovirus (mpv) are two of the most common causes of serious viral lower respiratory tract illness in humans. cd8+ t cells have been shown to be important in animal models and human clinical studies for the clearance of viral infection, and they may contribute in part to protection against severe disease during reinfections. precise enumeration and accurate phenotyping of rsv- or mpv-specific cd8+ t cells in humans is currently limited by t ... | 2011 | 21854782 |
| [Study of xiaozhang recipe combined with lamivudine in treatment of 84 chronic viral hepatitis B patients with compensated liver cirrhosis]. | To assess the clinical efficacy of Xiaozhang Recipe (XR) in treatment of chronic viral hepatitis B patients with compensated liver cirrhosis. | 2011 | 22013800 |
| an alphavirus replicon-based human metapneumovirus vaccine is immunogenic and protective in mice and cotton rats. | human metapneumovirus (hmpv) is a recently discovered paramyxovirus that causes upper and lower respiratory tract infections in infants, the elderly, and immunocompromised individuals worldwide. here, we developed venezuelan equine encephalitis virus replicon particles (vrps) encoding hmpv fusion (f) or attachment (g) glycoproteins and evaluated the immunogenicity and protective efficacy of these vaccine candidates in mice and cotton rats. vrps encoding hmpv f protein, when administered intranas ... | 2008 | 18786987 |
| monkeypox virus evades antiviral cd4+ and cd8+ t cell responses by suppressing cognate t cell activation. | monkeypox virus (mpv) is a virulent human pathogen that has gained increased attention because of its potential use as a bioterrorism agent and inadvertent introduction into north america in 2003. the us outbreak also provided an important opportunity to study mpv-specific t cell immunity. although mpv-specific cd4(+) and cd8(+) t cells could recognize vaccinia virus (vv)-infected monocytes and produce inflammatory cytokines such as ifngamma and tnfalpha, they were largely incapable of respondin ... | 2008 | 18796610 |
| age-associated variability in susceptibility of swiss webster mice to mpv and other excluded murine pathogens. | detection of mouse parvovirus (mpv) and other murine pathogens in research colonies is dependent on the transmissibility of the agents and the sensitivity of sentinels to those agents. transmissibility is based on several agent-dependent properties including mode of transmission, infectivity, and environmental stability, whereas host susceptibility can vary according to mouse age, strain, and sex. in this study, 4-wk-old, 12-wk-old, and aged swiss webster female sentinel mice were compared for t ... | 2012 | 23294885 |
| [Etiology survey on virus of acute respiratory infection in Guangzhou from 2006 to 2009]. | To investigate the pathogens of acute respiratory infection (ARI) in Guangzhou from 2006 to 2009. | 2011 | 22177307 |
| progress in respiratory virus vaccine development. | viral respiratory infections cause significant morbidity and mortality in infants and young children as well as in at-risk adults and the elderly. although many viral pathogens are capable of causing respiratory disease, vaccine development has to focus on a limited number of pathogens, such as those that commonly cause serious lower respiratory illness (lri). whereas influenza virus vaccines have been available for some time (see the review by clark and lynch in this issue), vaccines against ot ... | 2011 | 21858754 |
| optimization of peptide-based elisa for serological diagnostics: a retrospective study of human monkeypox infection. | abstract although smallpox has been eradicated, other diseases caused by virulent orthopoxviruses such as monkeypox virus (mpv) remain endemic in remote areas of western and central sub-saharan africa, and represent a potential biothreat due to international travel and/or inadvertent exposure. unfortunately, extensive antigenic cross-reactivity among orthopoxviruses presents a challenge to serological diagnosis. we previously reported a 20mer peptide-based elisa that identified recent mpv infe ... | 2012 | 22217169 |
| complete genome sequence and biological characterization of moroccan pepper virus (mpv) and reclassification of lettuce necrotic stunt virus as mpv. | moroccan pepper virus (mpv) and lettuce necrotic stunt virus (lnsv) have been steadily increasing in prevalence in central asia and western north america, respectively, over the past decade. recent sequence analysis of lnsv demonstrated a close relationship between the coat proteins of lnsv and mpv. to determine the full extent of the relationship between lnsv and mpv, the genomes of three mpv isolates were sequenced and compared with that of lnsv. sequence analysis demonstrated that genomic nuc ... | 2013 | 23360531 |
| the genome sequence of lettuce necrotic stunt virus indicates a close relationship to moroccan pepper virus. | lettuce necrotic stunt virus (lnsv) causes severe losses to lettuce production in the western united states, which results in stunting, necrosis and death on all non-crisphead lettuces, as well as flower abortion and yield losses in greenhouse tomato production. the genome of lnsv was sequenced and has an organization typical of viruses of the genus tombusvirus. sequence comparisons indicated that much of the genome is relatively closely related to tomato bushy stunt virus; however, the coat pro ... | 2012 | 22527864 |