| the p19 protein of grapevine algerian latent virus is a determinant of systemic infection of chenopodium quinoa. | a previous study showed that both grapevine algerian latent virus (galv) and tomato bushy stunt virus (tbsv) systemically infect nicotiana benthamiana, but galv causes systemic infection whereas tbsv causes only local lesions in chenopodium quinoa (c. quinoa). we recently isolated galv strain naju (galv-n) from limonium sinense and tbsv strain sacheon (tbsv-s) from tomato. both viruses belong to the genus tombusvirus and have a similar genome organization. to identify determinants of systemic in ... | 2012 | 22342277 |
| development of virus-induced gene expression and silencing vector derived from grapevine algerian latent virus. | grapevine algerian latent virus (galv) is a member of the genus tombusvirus in the tombusviridae and infects not only woody perennial grapevine plant but also herbaceous nicotiana benthamiana plant. in this study, we developed galv-based gene expression and virus-induced gene silencing (vigs) vectors in n. benthamiana. the galv coat protein deletion vector, pgmg, was applied to express the reporter gene, green fluorescence protein (gfp), but the expression of gfp was not detected due to the necr ... | 2016 | 27493613 |
| construction of a synthetic infectious cdna clone of grapevine algerian latent virus (galv-nf) and its biological activity in nicotiana benthamiana and grapevine plants. | grapevine algerian latent virus (galv) is a tombusvirus first isolated in 1989 from an algerian grapevine (vitis spp.) plant and more recently from water samples and commercial nipplefruit and statice plants. no further reports of natural galv infections in grapevine have been published in the last two decades, and artificial inoculations of grapevine plants have not been reported. we developed and tested a synthetic galv construct for the inoculation of nicotiana benthamiana plants and differen ... | 2014 | 25367743 |
| evaluation of various species demarcation criteria in attempts to classify ten new tombusvirus isolates. | the usefulness of various suggested species demarcation criteria was compared in attempts to determine the taxonomic status of ten new tombusvirus isolates. five of them (lim 1, 2, 3, 5 and 6) were obtained from different sources of commercially grown statice (limonium sinuatum), two (gyp 1 and 2) from different sources of commercially grown gypsophila paniculata and three from water samples, i.e. from a small river (schunter) in northern germany, from a brook (near dossenheim) in southern germa ... | 2004 | 15593416 |