Publications

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diversity and distribution of begomoviruses infecting tomato in india.leaf curl begomoviruses cause serious yield losses to indian tomato crops. total dnas were extracted from leaves of 69 tomato plants and 34 weeds or neighbouring crops collected from all the major tomato producing areas of india. eighty-one of the 103 samples were positive by pcrs using begomovirus genus-specific primers. coat protein (cp) genes from 29 samples were pcr amplified, cloned and sequenced. phylogenetic analyses of the cp sequences revealed five different tomato leaf curl begomovirus ...200515703846
molecular characterization of two distinct monopartite begomoviruses infecting tomato in india.tomato leaf curl viruses, which are the members of the genus begomovirus, have emerged as devastating pathogens worldwide causing huge economic losses and threatening production of crops like cassava, cotton, grain legumes and vegetables. even though the tolcv isolates from northern india have been shown to possess bipartite genome (designated as dna a and dna b), those from australia, taiwan and southern india have a single genomic component (dna a). we describe here the genetic diversity of tw ...201021092241
complete nucleotide sequence of croton yellow vein mosaic virus and dna-β associated with yellow vein mosaic disease of jatropha gossypifolia in india.a severe yellow vein mosaic disease was noticed on several jatropha gossypifolia plants growing nearby agriculture fields at lucknow, india. diseased plants exhibited yellow vein mosaic, leaf deformation, vein swelling and stunting. a population of whiteflies (bemisia tabaci) was also noticed in the vicinities; therefore, begomovirus infection was suspected. to confirm begomovirus association, total dna was isolated from symptomatic leaf samples and subjected to pcr using dna-a, dna-b and dna-β- ...201121479677
rnai mediated gene silencing against betasatellite associated with croton yellow vein mosaic begomovirus.plant viruses encode suppressors of posttranscriptional gene silencing, an adaptive antiviral defense responses that confines virus infection. previously, we identified single-stranded dna satellite (also known as dna-β) of ~1,350 nucleotides in length associated with croton yellow vein mosaic begomovirus (cyvmv) in croton plants. the expression of genes from dna-β requires the begomovirus for packaged, replication, insect transmission and movement in plants. the present study demonstrates the e ...201425086625
spatial and temporal diversity of begomoviral complexes in papayas with leaf curl disease.old world, monopartite begomoviruses associated with satellite dna β were observed in papaya showing symptoms of leaf curl disease sampled randomly over five years from within a radius of 250 km in north-central india. three groups of dna a sequences were evident. one group resembled chili leaf curl virus infecting tomatoes (chilcuv). another group resembled tomato leaf curl new delhi virus (tolcundv). the third group was novel (tentatively named papaya leaf crumple virus, palcrv), with less tha ...201222437254
complete nucleotide sequences of two begomoviruses infecting madagascar periwinkle (catharanthus roseus) from pakistan.though catharanthus roseus (madagascar periwinkle) is an ornamental plant, it is famous for its medicinal value. its alkaloids are known for anti-cancerous properties, and this plant is studied mainly for its alkaloids. here, this plant has been studied for its viral diseases. complete dna sequences of two begomoviruses infecting c. roseus originating from pakistan were determined. the sequence of one begomovirus (clone kn4) shows the highest level of nucleotide sequence identity (86.5 %) to an ...201323065111
host range and genetic diversity of croton yellow vein mosaic virus, a weed-infecting monopartite begomovirus causing leaf curl disease in tomato.croton yellow vein mosaic virus (cyvmv) is a widely occurring begomovirus in croton bonplandianum, a common weed in the indian subcontinent. in this study, cyvmv (genus begomovirus, family geminiviridae) was transmitted by whiteflies (bemisia tabaci) to as many as 35 plant species belonging to 11 families, including many vegetables, tobacco varieties, ornamentals and weeds. cyvmv produced bright yellow vein symptoms in croton, whereas in all the other host species, the virus produced leaf curl s ...201323096697
biology and interactions of two distinct monopartite begomoviruses and betasatellites associated with radish leaf curl disease in india.emerging whitefly transmitted begomoviruses are major pathogens of vegetable and fibre crops throughout the world, particularly in tropical and sub-tropical regions. mutation, pseudorecombination and recombination are driving forces for the emergence and evolution of new crop-infecting begomoviruses. leaf curl disease of field grown radish plants was noticed in varanasi and pataudi region of northern india. we have identified and characterized two distinct monopartite begomoviruses and associate ...201222339942
the efficacy of antisense-based construct for inducing resistance against croton yellow vein mosaic virus in nicotiana tabacum.begomoviruses have increased pathogenicity because of their adaptation to a wide host range; consequently, these viruses cause a major loss to agroeconomic crops worldwide. in this study, we designed a gene construct representing an antisense coat protein gene. we also analyzed the efficacy of the induced resistance against croton yellow vein mosaic virus (cryvmv) affecting papaya in nicotiana tabacum plants. positive control plants developed typical leaf curl symptoms, whereas transgenic plants ...201728762207
agroinfection of tobacco by croton yellow vein mosaic virus and designing of a replicon vector for expression of foreign gene in plant.croton yellow vein mosaic virus (cyvmv, genus begomovirus family geminiviridae) is a proliferating begomovirus in the indian sub-continent. the infectious constructs in binary vector was developed against the cyvmv genome and its associated betasatellite. agroinoculation of the genomic construct of cyvmv produced leaf curl symptoms alone in three species of tobacco, nicotiana tabacum, n. benthamiana and n. glutinosa. co-inoculation of betasatellite enhanced the severity of the disease and reduce ...201628466040
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