anaerostipes caccae gen. nov., sp. nov., a new saccharolytic, acetate-utilising, butyrate-producing bacterium from human faeces.two strains of a previously undescribed eubacterium-like bacterium were isolated from human faeces. the strains are gram-variable, obligately anaerobic, catalase negative, asporogenous rod-shaped cells which produced acetate, butyrate and lactate as the end products of glucose metabolism. the two isolates displayed 99.9% 16s rrna gene sequence similarity to each other and treeing analysis demonstrated the faecal isolates are far removed from eubacterium sensu stricto and that they represent a ne ...200212086188
lactate-utilizing bacteria, isolated from human feces, that produce butyrate as a major fermentation product.the microbial community of the human colon contains many bacteria that produce lactic acid, but lactate is normally detected only at low concentrations (<5 mm) in feces from healthy individuals. it is not clear, however, which bacteria are mainly responsible for lactate utilization in the human colon. here, bacteria able to utilize lactate and produce butyrate were identified among isolates obtained from 10(-8) dilutions of fecal samples from five different subjects. out of nine such strains ide ...200415466518
two routes of metabolic cross-feeding between bifidobacterium adolescentis and butyrate-producing anaerobes from the human gut.dietary carbohydrates have the potential to influence diverse functional groups of bacteria within the human large intestine. of 12 bifidobacterium strains of human gut origin from seven species tested, four grew in pure culture on starch and nine on fructo-oligosaccharides. the potential for metabolic cross-feeding between bifidobacterium adolescentis and lactate-utilizing, butyrate-producing firmicute bacteria related to eubacterium hallii and anaerostipes caccae was investigated in vitro. e. ...200616672507
cross-feeding between bifidobacterium longum bb536 and acetate-converting, butyrate-producing colon bacteria during growth on vitro coculture fermentations of bifidobacterium longum bb536 and two acetate-converting, butyrate-producing colon bacteria, anaerostipes caccae dsm 14662 and roseburia intestinalis dsm 14610, with oligofructose as the sole energy source, were performed to study interspecies interactions. two clearly distinct types of cross-feeding were identified. a. caccae dsm 14662 was not able to degrade oligofructose but could grow on the fructose released by b. longum bb536 during oligofructose breakdow ...200617056678
exopolysaccharides produced by intestinal bifidobacterium strains act as fermentable substrates for human intestinal bacteria.eleven exopolysaccharides (eps) isolated from different human intestinal bifidobacterium strains were tested in fecal slurry batch cultures and compared with glucose and the prebiotic inulin for their abilities to act as fermentable substrates for intestinal bacteria. during incubation, the increases in levels of short-chain fatty acids (scfa) were considerably more pronounced in cultures with eps, glucose, and inulin than in controls without carbohydrates added, indicating that the substrates a ...200818539803
isolation of lactate-utilizing butyrate-producing bacteria from human feces and in vivo administration of anaerostipes caccae strain l2 and galacto-oligosaccharides in a rat model.lactate-utilizing butyrate-producers were isolated from human feces and identified based on the sequences of 16s rrna gene. anaerostipes caccae strain l2, one of the seven human fecal isolates, was administered to rats with galacto-oligosaccharides (gos) as bifidogenic carbohydrates for stimulating lactate formation in the hindgut. ingestion of gos alone increased concentrations of cecal lactate and butyrate compared with control rats (p<0.05). additional administration of strain l2 on gos tende ...200818554304
in vitro kinetics of prebiotic inulin-type fructan fermentation by butyrate-producing colon bacteria: implementation of online gas chromatography for quantitative analysis of carbon dioxide and hydrogen gas production.kinetic analyses of bacterial growth, carbohydrate consumption, and metabolite production of five butyrate-producing clostridial cluster xiva colon bacteria grown on acetate plus fructose, oligofructose, inulin, or lactate were performed. a gas chromatography method was set up to assess h2 and co2 production online and to ensure complete coverage of all metabolites produced. method accuracy was confirmed through the calculation of electron and carbon recoveries. fermentations with anaerostipes c ...200919633122
lactate has the potential to promote hydrogen sulphide formation in the human colon.high concentrations of sulphide are toxic for the gut epithelium and may contribute to bowel disease. lactate is a favoured cosubstrate for the sulphate-reducing colonic bacterium desulfovibrio piger, as shown here by the stimulation of sulphide formation by d. piger dsm749 by lactate in the presence of sulphate. sulphide formation by d. piger was also stimulated in cocultures with the lactate-producing bacterium bifidobacterium adolescentis l2-32. other lactate-utilizing bacteria such as the bu ...200919732152
human intestinal microbiota: characterization of a simplified and stable gnotobiotic rat model.the study of host microbe interactions is hampered by the complexity and inter-individual variability of the human gut microbiota. therefore, a simplified human intestinal microbiota (sihumi) consisting of seven bacterial species was introduced into germfree rats. species selection was based on numerical importance and fermentative abilities in the human gut. association of the rats with the sihumi (anaerostipes caccae, bacteroides thetaiotaomicron, bifidobacterium longum, blautia producta, clos ...201021637015
arabinoxylans and inulin differentially modulate the mucosal and luminal gut microbiota and mucin-degradation in humanized rats.the endogenous gut microbiota affects the host in many ways. prebiotics should favour beneficial intestinal microbes and thus improve host health. in this study, we investigated how a novel class of potential prebiotic long-chain arabinoxylans (lc-ax) and the well-established prebiotic inulin (in) modulate the gut microbiota of humanized rats. six weeks after axenic rats were inoculated with a human faecal microbiota, their colonic microbiota was similar to this inoculum (∼ 70%), whereas their c ...201121883787
anaerostipes butyraticus sp. nov., an anaerobic, butyrate-producing bacterium from clostridium cluster xiva isolated from broiler chicken caecal content, and emended description of the genus anaerostipes.four butyrate-producing isolates were obtained from the caecal content of a 4-week-old broiler chicken. the 16s rrna gene sequences were determined and confirmed the close relatedness of the four isolates, which suggested that they were derived from a single bacterial clone. phylogenetic analysis based on 16s rrna gene sequences showed that its closest relatives were members of cluster xiva of the clostridium subphylum of gram-positive bacteria and that the closest related type strain was anaero ...201019666785
na(+)-translocating membrane pyrophosphatases are widespread in the microbial world and evolutionarily precede h(+)-translocating pyrophosphatases.membrane pyrophosphatases (ppases), divided into k(+)-dependent and k(+)-independent subfamilies, were believed to pump h(+) across cell membranes until a recent demonstration that some k(+)-dependent ppases function as na(+) pumps. here, we have expressed seven evolutionarily important putative ppases in escherichia coli and estimated their hydrolytic, na(+) transport and h(+) transport activities as well as their k(+) and na(+) requirements in inner membrane vesicles. four of these enzymes (fr ...201121527638
kinetic modelling of lactate utilization and butyrate production by key human colonic bacterial species.butyrate is the preferred energy source for colonocytes and has an important role in gut health; in contrast, accumulation of high concentrations of lactate is detrimental to gut health. the major butyrate-producing bacterial species in the human colon belong to the firmicutes. eubacterium hallii and a new species, anaerostipes coli ss2/1, members of clostridial cluster xiva, are able to utilize lactate and acetate via the butyryl coa : acetate coa transferase route, the main metabolic pathway f ...201121388423
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