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studies on erythrocyte membrane. vii. in vitro haemolytic effect of sesbania grandiflora leaves.aqueous extract of the leaves of sesbania grandiflora produced haemolysis of human and sheep erythrocytes even at very low concentrations. haemolysis was greater when the ph was acidic. the liberation of phospholipids and sterols into the supernatant as a result of haemolysis indicated possible damage to the erythrocyte membrane.19827080081
primary structure of sesbania mosaic virus coat protein: its implications to the assembly and architecture of the virus.sesbania mosaic virus (smv) is a plant virus that infects sesbania grandiflora plants in andhra pradesh, india. the amino acid sequence of the coat protein of smv was determined using purified peptides generated by cleavage with trypsin, chymotrypsin, v8 protease and clostripain. the 230 residues so far determined were compared to the corresponding residues of southern bean mosaic virus (sbmv), the type member of sobemoviruses. the overall identity between the sequences is 61.7%. the amino termi ...19948002015
structure of sesbania mosaic virus at 4.7 a resolution and partial sequence of the coat protein.sesbania mosaic virus (smv) is a plant virus infecting sesbania grandiflora plants in andhra pradesh, india. amino acid sequence of the tryptic peptides of smv coat protein were determined using a gas phase sequenator. these sequences showed identical amino acids at 69% of the positions when aligned with the corresponding residues of southern bean mosaic virus (sbmv). crystals diffracting to better than 3 a resolution were obtained by precipitating the virus with ammonium sulphate. the crystals ...19938421301
consumption pattern of carotene rich foods and development of a year calendar.consumption pattern of beta carotene rich foods from 500 households of coimbatore district was elicited. through market surveys in four seasons namely: summer, south-west monsoon, north-east monsoon and winter, a year calendar of beta carotene rich foods was developed. the total and beta carotene contents of five commonly consumed beta carotene rich foods both in raw and cooked states were determined. results indicated that greens were mainly purchased from market and consumed 2-3 times per week ...19968886334
structure of sesbania mosaic virus at 3 a resolution.sesbania mosaic virus (smv) is an isometric, ss-rna plant virus found infecting sesbania grandiflora plants in fields near tirupathi, south india. the virus particles, which sediment at 116 s at ph 5.5, swell upon treatment with edta at ph 7.5 resulting in the reduction of the sedimentation coefficient to 108 s. smv coat protein amino acid sequence was determined and found to have approximately 60% amino acid sequence identity with that of southern bean mosaic virus (sbmv). the amino terminal 60 ...19979468608
anxiolytic and anticonvulsive activity of sesbania grandiflora leaves in experimental animals.various parts of sesbania grandiflora have been used in the indian system of medicine, in particular, the leaves of s. grandiflora are used in ayurveda for the treatment of epileptic fits. in the present study we have evaluated the anticonvulsive activity of s. grandiflora leaves using a variety of animal models of convulsions. bioassay guided separation was also carried out to identify the fraction possessing anticonvulsant activity. the benzene:ethyl acetate fraction (be) of the acetone solubl ...200212203267
protective effect of sesbania grandiflora against erythromycin estolate-induced hepatotoxicity.sesbania grandiflora, commonly known as 'sesbania', is widely used in indian folk medicine for the treatment of liver disorders. oral administration of an ethanolic extract of s. grandiflora leaves (200 mg/kg/day) for 15 days produced significant hepatoprotection against erythromycin estolate (800 mg/kg/day)-induced hepatotoxicity in rats. the increased level of serum enzymes (aspartate transaminase, alanine transaminase, alkaline phosphatase), bilirubin, cholesterol, triglycerides, phospholipid ...200314682193
studies on the bioavailability of the provitamin a carotenoid, beta-carotene, using human exfoliated colonic epithelial cells.the possibility of using exfoliated colonic epithelial cells for assessing the bioavailability of beta-carotene was examined. analysis of exfoliated colonic epithelial cells showed the presence of beta-carotene and vitamin a. the beta-carotene content was significantly lower in cells from stool samples of subjects on a beta-carotene-poor diet than those receiving a single dose of a beta-carotene supplement. colonic epithelial cells isolated from stool samples collected daily during a wash-out pe ...200415333155
determination of major carotenoids in a few indian leafy vegetables by high-performance liquid chromatography.leafy vegetables [basella rubra l., peucedanum sowa roxb., moringa oleifera lam., trigonella foenum-graecum l., spinacia oleracea l., sesbania grandiflora (l.) poir., and raphanus sativus l.] that are commonly used by the rural population in india were evaluated in terms of their main carotenoid pattern. the extracted carotenoids were purified by open column chromatography (occ) on a neutral alumina column to verify their identity by their characteristic uv-visible absorption spectra. reverse-ph ...200515826027
alpha-glucosidase inhibitor proteins from sesbania grandiflora flowers.two alpha-glucosidase inhibitors were isolated from the flowers of sesbania grandiflora and named sgf60 and sgf90. the procedure involved extraction with phosphate buffer, precipitation with ammonium sulfate, ion-exchange chromatography on deae-cellulose and gel filtration on superdex-200. these proteins were identified by using tandem mass spectrometry. the results show partial amino acid sequences of sgf60 similar to p27sj, a protein from hypericum perforatum found to suppress hiv-1 gene expre ...200717823874
evaluation of sesbania grandiflora for antiurolithiatic and antioxidant properties.in the indigenous system of medicine in india, the plant sesbania grandiflora is claimed to be useful for various ailments, and one such use is for the treatment of renal calculi. the major purpose of this study is to investigate the potential of s. grandiflora in the treatment of renal calculi. the leaf juice of s. grandiflora was evaluated for median lethal dose, gross behavioral changes, antiurolithiatic and antioxidant activities. the antiurolithiatic activity was evaluated by a calculi-prod ...200818408896
protective effect of sesbania grandiflora against cigarette smoke-induced oxidative damage in rats.sesbania grandiflora, commonly known as "sesbania" and "agathi," is widely used in indian traditional medicine for the treatment of a broad spectrum of diseases. in the present study, we evaluated the possible protective effect of an aqueous suspension of s. grandiflora (assg) leaves against cigarette smoke-induced oxidative damage in rats. adult wistar-kyoto rats were exposed to cigarette smoke for a period of 90 days and treated with assg (1,000 mg/kg of body weight/day, p.o) for a period of 3 ...200818598182
cardioprotective effects of sesbania grandiflora in cigarette smoke-exposed rats.cigarette smoke is a major risk factor of coronary heart disease, myocardial infarction, and cardiac death. it has been reported to contain large amounts of oxidants. this study was undertaken to evaluate the cardioprotective effects of sesbania grandiflora (s. grandiflora) against cigarette smoke-induced oxidative damage in rats. adult male wistar-kyoto rats were exposed to cigarette smoke for a period of 90 days and consecutively treated with s. grandiflora aqueous suspension (sgas, 1000 mg/kg ...200818791462
a novel protein fraction from sesbania grandiflora shows potential anticancer and chemopreventive efficacy, in vitro and in vivo.we report mechanism-based evidence for the anticancer efficacy of a protein fraction, sf2 (sesbania fraction 2) isolated from the flower of the medicinal plant, sesbania grandiflora (s. grandiflora). the fraction was evaluated in two murine ascites tumour cell lines and human cancer cell lines of different origin for its anticancer effect. sf2 inhibited cell proliferation and induced apoptosis as demonstrated by dna fragmentation and externalization of phosphatidyl serine in daltons lymphoma asc ...201019183244
evaluation of tropical plants containing tannin on in vitro methanogenesis and fermentation parameters using rumen fluid.methane (ch(4)) produced during ruminal fermentation represents a loss of 10-11% of gross energy intake. the use of browse species containing tannin as feed supplement for ruminants tends to increase in order to reduce ch(4) production. the present study was conducted to evaluate some tropical plants containing tannin as feed supplement (200 g kg(-1)) on in vitro ch(4) production and fermentation parameters.201020355068
sesbania grandiflora diminishes oxidative stress and ameliorates antioxidant capacity in liver and kidney of rats exposed to cigarette smoke.cigarette smoke is a major risk factor for many chronic diseases. however, it may be possible to relieve the smoke-induced damage by increasing the defensive system. in this study, we planned to evaluate the protective mechanism of sesbania grandiflora (s. grandiflora) leaves against cigarette smoke-induced oxidative damage in liver and kidney of rats. adult male wistar-kyoto rats were exposed to cigarette smoke for a period of 90 days and consecutively treated with s. grandiflora aqueous suspen ...201020814075
evaluation of anticancer activity of ethanol extract of sesbania grandiflora (agati sesban) against ehrlich ascites carcinoma in swiss albino mice.ethno pharmacological relevance: the plant sesbania grandiflora (fabaceae) is commonly known as "sesbania" and "agathi" in ayurvedic system of medicine and reputed in the indigenous medicine in india. it is also known as "agati sesban" or "humming bird tree" in english. all parts of this unique plant are useful and have a wide spectrum of medicinal properties. the plant has various uses in folk and traditional medicines for headache, swellings, anemia, bronchitis, pains, liver disorders and tumo ...201121251969
screening for feeding deterrent activity of herbal extracts against the larvae of malaria vector anopheles subpictus grassi.this no-choice, laboratory study focuses on the feeding of homogeneous powdered, dried, yeast with different plant extracts on mosquito fourth-instar larvae to determine the effects on their mortality. screening for antifeedant activity of plant extracts with some known medicinal attributes could lead to the discovery of new agents for vector control. the aim of this study was to investigate the antifeedant activity of crude leaf hexane, ethyl acetate, acetone, and methanol extracts of andrograp ...201121373809
protective effects of sesbania grandiflora on kidney during alcohol and polyunsaturated fatty acid-induced oxidative stress.kidney is an organ highly vulnerable to damage caused by reactive oxygen species (ros), due to abundance of polyunsaturated fatty acids in the composition of renal lipids. oxidative stress plays an important role in the development of alcohol-induced tissue injury. this leads to membrane damage by lipid peroxidation, alteration of enzyme function and formation of acetaldehyde protein adducts. many alcoholics along with alcohol consume fried foods that are normally made up of polyunsaturated fatt ...201121417636
sesbania grandiflora leaf extract mediated green synthesis of antibacterial silver nanoparticles against selected human pathogens.simple, effective and rapid approach for the green synthesis of silver nanoparticles (agnps) using leaf extract of sesbania grandiflora and their in vitro antibacterial activity against selected human pathogens has been demonstrated in the study. various instrumental techniques were adopted to characterize the synthesized agnps viz. uv-vis, ftir, xrd, tem, edx and afm. surface plasmon spectra for agnps are centered at 422 nm with dark brown color. the synthesized agnps were found to be spherical ...201223270884
biochemical changes in germinating seeds of sesbania grandiflora pers. i. changes in carbohydrates and role of tegmen. 20134243409
investigation on antibacterial and antioxidant activities, phenolic and flavonoid contents of some thai edible plants as an alternative for antibiotics.this study was aimed to examine the antibacterial and antioxidative properties of seven edible plants from thailand to develop alternative antibiotics as feed additives. the plants include citrus aurantifolia swingle (lime) fruits and its leaves, sesbania grandiflora l. (agati sesbania) leaves, piper sarmentosum roxb (wild betal) leaves, curcuma domestica valeton (turmeric) roots, morinda citrifolia l. (beach mulberry) leaves, cassia siamea britt (siamea cassia) leaves, and cocos nucifera l. (co ...201425178298
in vitro antibacterial activity and in vivo therapeutic effect of sesbania grandiflora in bacterial infected silkworms.antibiotic resistance is a serious problem worldwide. searching for new potential agents is, therefore, essential. the bark of sesbania grandiflora (l.) pers. (fabaceae) has been used in folk medicine against various diseases.201728253823
antimicrobial activity of sesbania grandiflora flower polyphenol extracts on some pathogenic bacteria and growth stimulatory effect on the probiotic organism lactobacillus acidophilus.polyphenolic extracts (pe) of edible flower of sesbania grandiflora were tested to evaluate its antimicrobial effect against some common pathogenic bacteria and growth promoting property against probiotic organism lactobacillus acidophilus. the antimicrobial activity of s. grandiflora flower pe against selected pathogens was evaluated using both in vitro and in situ methods. in vitro studies suggested that pe has inhibitory effect against staphylococcus aureus, shigella flexneri 2a, salmonella t ...201222583547
the chemical components of sesbania grandiflora root and their antituberculosis activity.three isoflavanoids, isovestitol (1), medicarpin (2), and sativan (3), along with another known compound, betulinic acid (4), were isolated from the root of sesbania grandiflora. the structures of the isolated compounds were characterised by means of spectroscopic techniques (uv, ir, ms, 1h- and 13c-nmr, dept, cosy, hmqc, hmbc, and ms analysis). all the tested compounds 1-4 exhibited antituberculosis activity against mycobacterium tuberculosis h37rv, with mic values of 50 µg/ml for compounds 1-3 ...201224280680
antioxidant, antibacterial, and cytoprotective activity of agathi leaf protein.in the present study a protein termed agathi leaf protein (alp) from sesbania grandiflora linn. (agathi) leaves was isolated after successive precipitation with 65% ammonium sulphate followed by purification on sephadex g 75. the column chromatography of the crude protein resulted in four peaks of which peak i (p i) showed maximum inhibition activity against hydroxyl radical. sds-page analysis of p i indicated that the molecular weight of the protein is ≈ 29 kda. the purity of the protein was 98 ...201424616824
bioavailability of calcium and its absorption inhibitors in raw and cooked green leafy vegetables commonly consumed in india--an in vitro study.the objectives of this research were to assess the bioavailability of calcium using equilibrium dialysis after simulated gastric digestion method in 20 commonly consumed green leafy vegetables (glvs) from the typical indian diet, provide data on the content of calcium absorption inhibitors, like oxalate, phytate, tannin and dietary fibres, and evaluate the inhibitory effect of these compounds on calcium bioavailability in raw and cooked glvs. cooking did not affect significantly calcium bioavail ...201525306367
phytoremediation of trichloroethylene and dichlorodiphenyltrichloroethane-polluted water using transgenic sesbania grandiflora and arabidopsis thaliana plants harboring rabbit cytochrome p450 2e1.sesbania grandiflora (l.) pers (fabaceae) and arabidopsis thaliana (l.) (brassicaceae) were genetically engineered to constitutively express the rabbit cytochrome p450 2e1 enzyme aiming at increasing their activity toward trichloroethylene (tce) and dichlorodiphenyltrichloroethane (ddt) removal successful generation of sesbania and arabidopsis transgenic plants was verified using p450 2e1 specific pcr and confirmed by western blot analysis. gas chromatography (gc) analysis revealed that small cu ...201222908634
encapsulation of sesbania grandiflora extract in polymeric micelles to enhance its solubility, stability, and antibacterial activity.clinical applications of sesbania grandiflora bark extract (sge) are limited because of its poor water solubility and stability. sge was loaded in micelles of pluronics. in vitro and in vivo antibacterial and toxicity tests were investigated using broth dilution and silkworm model. aqueous solubility of sge was improved by these micelles. activity and toxicity of sge loaded micelles were dependent on type and concentration of pluronics. the micelles composed of 1:3 sge to pluronic f68 (sge-pf68- ...201728097930
total phenolic compounds, flavonoids, and radical scavenging activity of 21 selected tropical plants.free radical scavenging activity of 21 tropical plant extracts was evaluated using 1,1-diphenyl-2-picrylhydrazyl assay (dpph). total phenolic compounds and flavonoids were determined using folin-ciocalteu and hplc, respectively. results of the study revealed that all the plants tested exhibited excellent antioxidant activity with ic(50) in the range of 21.3 to 89.6 microg/ml. the most potent activity was demonstrated by cosmos caudatus (21.3 microg/ml) and piper betle (23.0 microg/ml) that are n ...201420492146
sesbania mosaic virus (semv) infectious clone: possible mechanism of 3' and 5' end repair and role of polyprotein processing in viral replication.sesbania mosaic virus (semv) is a positive stranded rna virus belonging to the genus sobemovirus. construction of an infectious clone is an essential step for deciphering the virus gene functions in vivo. using agrobacterium based transient expression system we show that semv icdna is infectious on sesbania grandiflora and cyamopsis tetragonoloba plants. the efficiency of icdna infection was found to be significantly high on cyamopsis plants when compared to that on sesbania grandiflora. the coa ...201222355344
nutrient content, in vitro ruminal fermentation characteristics and methane reduction potential of tropical tannin-containing leaves.plant tannins as rumen modifiers are better than chemicals or antibiotic-based modifiers since these compounds are natural products which are environmentally friendly and therefore have a better acceptance with regard to feed safety issues. tropical plants containing phenols such as tannins were found to suppress or eliminate protozoa from the rumen and reduce methane and ammonia production. the screening of these plants is an important step in the identification of new compounds and feed additi ...201222522493
mycorrhizal inoculant alleviates salt stress in sesbania aegyptiaca and sesbania grandiflora under field conditions: evidence for reduced sodium and improved magnesium uptake.a field experiment was conducted to examine the effect of the arbuscular mycorrhizal fungus glomus macrocarpum and salinity on growth of sesbania aegyptiaca and s. grandiflora. in the salt-stressed soil, mycorrhizal root colonisation and sporulation was significantly higher in am-inoculated than in uninoculated plants. mycorrhizal seedlings had significantly higher root and shoot dry biomass production than non-mycorrhizal seedlings grown in saline soil. the content of chlorophyll was greater in ...200414574620
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