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patterns of carbon partitioning in leaves of crassulacean acid metabolism species during deacidification.carbohydrates stored during deacidification in the light were examined in 11 crassulacean acid metabolism (cam) species from widely separated taxa grown under uniform conditions. the hypothesis that nad(p) malic enzyme cam species store chloroplastic starch and glucans, and phosphoenolpyruvate carboxykinase species store extrachloroplastic sugars or polymers was disproved. of the six malic enzyme species examined, kalanchoe tubiflora, kalanchoe pinnata, kalanchoe daigremontiana, and vanilla plan ...199612226397
floral benzenoid carboxyl methyltransferases: from in vitro to in planta function.benzenoid carboxyl methyltransferases synthesize methyl esters (e.g., methyl benzoate and methyl salicylate), which are constituents of aromas and scents of many plant species and play important roles in plant communication with the surrounding environment. within the past five years, eleven such carboxyl methyltransferases were isolated and most of them were comprehensively investigated at the biochemical, molecular and structural level. two types of enzymes can be distinguished according to th ...200515946712
malate metabolism in hoya carnosa mitochondria and its role in photosynthesis during cam phase iii.this study investigated the respiratory properties and the role of the mitochondria isolated from one phosphoenolpyruvate carboxykinase (pck)-cam plant, hoya carnosa, in malate metabolism during cam phase iii. the mitochondria showed high malate dehydrogenase (mmdh) and aspartate amino transferase (mast), and a significant amount of malic enzyme (mme) activities. h. carnosa readily oxidized malate via mme and mmdh in the presence of some cofactors such as thiamine pyrophosphate (tpp), coenzyme a ...200818403382
oxygen-18 incorporation into malic acid during nocturnal carbon dioxide fixation in crassulacean acid metabolism plants. a new approach to estimating in vivo carbonic anhydrase activity.crassulacean acid metabolism (cam) plants fix carbon dioxide at night by the carboxylation of phosphoenolpyruvate. if co2 fixation is conducted with 13c18o2 , then in the absence of carbonic anhydrase, the malate formed by dark co2 fixation should also contain high levels of carbon-13 and oxygen-18. conversely, if carbonic anhydrase is present and highly active, oxygen exchange between co2 and cellular h2o will occur more rapidly than carboxylation, and the [13c] malate formed will contain littl ...19846427227
further observations on rhythmic emission of fragrance in flowers.observations regarding floral fragrance and the rhythmicity of its emission in four plant species are reported. in the case of flowers of hoya carnosa r. br. which are characterized by circadian rhythmicity of scentedness (r. altenburger and p. matile, 1988, planta 174, 248-252), temperature compensation of the free-running period as well as persistence of oscillations in permanent darkness have been demonstrated. a hitherto unidentified component of fragrance turned out to be identical to an un ...199024201944
rhythms of fragrance emission in flowers.a method for the sampling of volatiles emitted by individual flowers is described. sampling over periods of 3 h allowed the examination of diurnal changes in quantity and quality of fragrance. in the species studied, odontoglossum constrictum lindl., citrus medica l., hoya carnosa r. br., and stephanotis floribunda brongs., the fragrance was characterized by a few major components accompanied by a larger number of minor components. flowers of all species produced volatiles in a rhythmical, diurn ...198824221481
toward in vivo chemical imaging of epicuticular waxes.epicuticular waxes, which are found on the outer surface of plant cuticles, are difficult to study in vivo. to monitor the growth, development, and structural alterations of epicuticular wax layers, coherent anti-stokes raman scattering (cars) might be used. cars, as a raman-based technique, not only provides structural insight but also chemical information by imaging the spatial distribution of raman-active vibrations. here, we present a comparative study using cars and scanning electron micros ...201020709828
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