| molecular characterization of a bovine enteric calicivirus: relationship to the norwalk-like viruses. | jena virus (jv) is a noncultivatable bovine enteric calicivirus associated with diarrhea in calves and was first described in jena, germany. the virus was serially passaged 11 times in colostrum-deprived newborn calves and caused diarrheal disease symptoms at each passage. the complete jv genome sequence was determined by using cdna made from partially purified virus obtained from a single stool sample. jv has a positive-sense single-stranded rna genome which is 7,338 nucleotides in length, excl ... | 1999 | 9847396 |
| characterisation of the bovine enteric calici-like virus, newbury agent 1. | the bovine enteric calici-like virus, newbury agent 1 (na1) was characterised to determine if it is a member of the caliciviridae and to establish its antigenic relationship to the established bovine enteric calicivirus newbury agent 2 (na2). solid phase immune electron microscopy (spiem) allowed quantification of na1 virions and identification of faecal samples with optimal virus levels. na1 particles were 36.6 nm in diameter, had an indefinite surface structure resembling that of human small r ... | 2000 | 11040440 |
| studies of epidemiology and seroprevalence of bovine noroviruses in germany. | jena virus (jv) is a bovine enteric calicivirus that causes diarrhea in calves. the virus is approximately 30 nm in diameter and has a surface morphology similar to the human norwalk virus. the genome sequence of jv was recently described, and the virus has been assigned to the genus norovirus of the family caliciviridae: in the present study, the jv capsid gene encoded by open reading frame 2 was cloned into the baculovirus transfer vector pfastbac 1, and this was used to transform escherichia ... | 2003 | 12791840 |
| [molecular identification of bovine enteric calciviruses in venezuela]. | caliciviruses are a well-established cause of respiratory, vesicular and hemorrhagic diseases in animals. in addition, these viruses are an important cause of enteric diseases in humans. recently, molecular analysis of several bovine enteric calicivirus isolates indicated that they are genetically close to human enteric calicivirus. to investigate if bovine enteric caliciviruses circulate in venezuela, 129 stool samples collected between 1994 and 2000 were assayed by reverse transcription-polyme ... | 2003 | 14976786 |
| a chimeric bovine enteric calicivirus: evidence for genomic recombination in genogroup iii of the norovirus genus of the caliciviridae. | the norovirus genus of the caliciviridae encompasses viruses that cause outbreaks of gastroenteritis in human and viruses that have been associated with diarrhea in cattle. the two bovine noroviruses, bo/newbury2/76/uk and bo/jena/80/de, represent two distinct genetic clusters in the newly described genogroup iii. in the present study, jena-like polymerase sequences were identified for the first time in the uk, but one of these, bo/thirsk10/00/uk, was a chimeric virus. bo/thirsk10/00/uk had a je ... | 2004 | 15302209 |
| molecular detection and characterization of unclassified bovine enteric caliciviruses in south korea. | the unclassified bovine enteric calicivirus (bec) is a new bovine enteric calicivirus that is different from bovine norovirus, and causes diarrhea and pathologies in the small intestine of calves. this virus includes nebraska (nb)- and newbury agent 1 (na1)-like strains. the prevalence of this bec and its genetic characterization has only been reported in the uk and the usa. this study examined the prevalence and genetic diversity of these becs in diarrheic calves in south korea. among a total o ... | 2008 | 18387758 |
| development of sybr green real-time rt-pcr for rapid detection, quantitation and diagnosis of unclassified bovine enteric calicivirus. | unclassified bovine enteric calicivirus (becv) is a newly recognized bovine enteric calicivirus that differs from bovine norovirus, and which causes diarrhea in the small intestines of calves. to date, methods such as real-time reverse transcription-polymerase chain reaction (rt-pcr) have not been developed for the rapid detection, quantitation and diagnosis of becv. presently, a becv-specific sybr green real-time rt-pcr assay was evaluated and optimized. diarrheic specimens (n=118) collected fr ... | 2009 | 19442847 |
| enteric campylobacteria and rna viruses associated with healthy and diarrheic humans in the chinook health region of southwestern alberta, canada. | the presence of campylobacter species and enteric rna viruses in stools from diarrheic (n = 442) and healthy (n = 58) humans living in southwestern alberta was examined (may to october 2005). a large number of diarrheic individuals who were culture negative for c. jejuni (n = 54) or c. coli (n = 19) were pcr positive for these taxa. overall detection rates for c. jejuni and c. coli in diarrheic stools were 29% and 5%, respectively. in contrast, 3% and 0% of stools from healthy humans were positi ... | 2010 | 21106791 |
| putative vaccine breakthrough event associated with heterotypic rotavirus infection in newborn calves, turkey, 2015. | group a rotaviruses (rva) are regarded as major enteric pathogens of large ruminants, including cattle. rotavirus vaccines administered to pregnant cows are commonly used to provide passive immunity that protects newborn calves from the clinical disease. in this study we report the detection of rva from calves with severe diarrhea in a herd regularly vaccinated to prevent enteric infections including rva. diarrheic disease was observed in newborn calves aged 4-15days, with high morbidity and mor ... | 2017 | 28284625 |
| numbers of coliforms, escherichia coli, f-rna phage, rotavirus, bovine enteric calicivirus and presence of non-o157 stec on commercial vacuum packaged beef. | the numbers of coliforms, escherichia coli, f-rna coliphages, bovine enteric calicivirus (bec) and rotavirus (rv) and presence of non-o157 shiga toxigenic e. coli (stec) were determined on commercial vacuum packaged beef subprimals at the retail level from swabs obtained from the entire surfaces of 150 cuts that originated from federally and provincially registered plants. the prevalence and log mean numbers of e. coli were higher in provincially registered plants than in federally registered pl ... | 2014 | 24929741 |
| identification of a bovine enteric calicivirus, kırklareli virus, distantly related to neboviruses, in calves with enteritis in turkey. | a calicivirus was detected in neonatal calves with enteritis in kırklareli, thrace, turkey. in the full-length genome, kırklareli virus was related (48% nucleotide identity) to bovine enteric caliciviruses (nebovirus genus). the virus was also detected in a herd in ankara, central anatolia, but not in other turkish prefectures. | 2015 | 26292294 |