| characterization of the rna-binding regions in protein p36 of heliothis armigera cypovirus 14. | some proteins of cypovirus (cpv) bind to rna, probably contributing to the replication of viral genome. however, little is known about whether any protein from heliothis armigera cypovirus (hacpv) could bind to rna. in this study, we cloned the orf of segment 9 (s9) of hacpv, serotype 14, into pmal-c2x for the generation and purification of maltose binding protein (mbp) fused protein p36 (mbp-p36). the analysis of the rna-binding properties of mbp-p36 revealed that p36, but not mbp alone, bound ... | 2007 | 17350708 |
| comparison of the amino acid sequences of rna-dependent rna polymerases of cypoviruses in the family reoviridae. | the nucleotide sequences of the genome segment s2 of bombyx mori cypovirus 1, s2 of lymantria dispar cypovirus 1, s1 of lymantria dispar cypovirus 14 and s1 of a proposed new electropherotype of trichoplusia ni cypovirus 15 were determined. these segments encoded putative rna-dependent rna polymerases (rdrps). the deduced amino acid sequences of rdrps within the genus cypovirus showed 32% to 94% identities, while extent of homology between rdrps in the genera cypovirus and oryzavirus, a genus mo ... | 2003 | 12556988 |