| nucleotide sequences of genome segments 6 and 7 of bombyx mori cypovirus 1, encoding the viral structural proteins v4 and v5, respectively. | nucleotide sequence analyses of cdnas derived from the double-stranded rna genome segments 6 and 7 (s6 and s7) of bombyx mori cypovirus 1 (bmcpv-1) have revealed that they consist of 1796 and 1501 nucleotides encoding putative proteins of 561 and 448 amino acids with molecular masses of 63604 and 49875 (p64 and p50), respectively. the amino acid sequence of p64, which has a high leucine residue content (10%), contains a leucine zipper motif. antiserum raised against p64 specifically bound to a v ... | 2000 | 10725444 |
| nucleotide sequence of genome segment 5 from bombyx mori cypovirus 1. | the complete nucleotide sequences of the double-stranded rna genome segments 5 (s5) from bombyx mori cypovirus 1 (bmcpv-1) strains i and h were determined. the segments consisted of 2,852 nucleotides encoding putative proteins of 881 amino acids with molecular masses of approximately 101 kda (p101). a homology search showed that p101 has high similarity (93%) to foot-and-mouth disease virus (fmdv) 2a protease (2apro) at amino acid position 219 to 235. these findings suggest the possibility that ... | 2001 | 11266213 |
| characterization of cypovirus isolates from tropical and temperate indian saturniidae silkworms. | cytoplasmic polyhedrosis virus (cypovirus, cpv) isolates from larvae of saturniidae silkworms antheraea mylitta, antheraea proylei, and antheraea assamensis were obtained and characterized. scanning electron microscopic (sem) studies revealed the presence of hexahedral (rhombic dodecahedron) polyhedral bodies of various size (1-4 microns) in individual hosts while transmission electron microscopic (tem) studies of isolated virions showed their uniform (icosahedral) shape and size (approximately ... | 2000 | 11332278 |
| nucleotide sequences of segments 1, 3 and 4 of the genome of bombyx mori cypovirus 1 encoding putative capsid proteins vp1, vp3 and vp4, respectively. | the complete nucleotide sequences of genomic segments s1, s3 and s4 from bombyx mori cypovirus 1 (bmcpv-1) have been determined. the segments consisted of 4190, 3846 and 3262 nucleotides encoding putative proteins of 1333, 1239 and 1058 amino acids with molecular masses of approximately 148, 140 and 120 kda (p148, p140 and p120, respectively). all segments possess a single open reading frame. homology searches showed that all three proteins have homologies to proteins of rice ragged stunt virus, ... | 2002 | 12029163 |
| genomic sequence analyses of segments 1 to 6 of dendrolimus punctatus cytoplasmic polyhedrosis virus. | the complete nucleotide sequences of genomic segments s1 to s6 from dendrolimus punctatus cypovirus 1 (dpcpv-1) have been determined. each segment of s1 to s6 possess a single open reading frame. conserved motifs 5' (aguaa) and 3'(guuagcc) were found at the ends of each segment. comparison of the proteins of dpcpv with those of other members in the family reoviridae lead us to suggest that s1, s3, s4 and s6 encode the viral structural protein vp1, vp2, vp3 and vp4, respectively. s5 encoded viral ... | 2003 | 12827465 |
| assembly into single-shelled virus-like particles by major capsid protein vp1 encoded by genome segment s1 of bombyx mori cypovirus 1. | the major capsid protein vp1 encoded by genome segment s1 of bombyx mori cypovirus 1 was expressed in a baculovirus system. in the absence of any other capsid proteins, vp1 was found to assemble into single-shelled virus-like particles. the vp1 particles were more sensitive to acidic conditions than were intact particles. | 2003 | 12917465 |
| nucleotide sequences of genome segments s6, s7 and s10 of dendrolimus punctatus cypovirus 1. | the nucleotide sequences of genome segments s6, s7 and s10 of dendrolimus punctatus cypovirus 1 hunan i (dpcpv-hn(i)) and dpcpv-hn(i)-se(3) (dpcpv-hn(i) passed three times in spodoptera exigua) were determined. segment s10 was 944 nucleotides in length and encoded a polyhedrin of 248 amino acids (28,439 da). only two nucleotide mutations were found between dpcpv-hn(i) s10 and dpcpv-hn(i)-se3 s10, and the deduced amino acid sequences of the polyhedrin proteins were identical. segment s7, 1 501 nu ... | 2004 | 14689283 |
| entry of bombyx mori cypovirus 1 into midgut cells in vivo. | in vivo entry of bombyx mori cypovirus 1 into the silkworm midgut was studied by electron microscopy of ultrathin sections of midguts from silkworm larvae that had been administered virus-contaminated leaves. in 3 h, virions were observed outside and inside midgut cells, including columnar cells, goblet cells and muscle cells. virions were seen adhering to the plasma membrane of microvilli, embedding in the membrane and settling themselves intact inside the microvilli of the columnar cells. thes ... | 2003 | 14700081 |
| bioinformatic analysis suggests that the cypovirus 1 major core protein cistron harbours an overlapping gene. | members of the genus cypovirus (family reoviridae) are common pathogens of insects. these viruses have linear dsrna genomes divided into 10-11 segments, which have generally been assumed to be monocistronic. here, bioinformatic evidence is presented for a short overlapping coding sequence (cds) in the cypovirus genome segment encoding the major core capsid protein vp1, overlapping the 5'-terminal region of the vp1 orf in the +1 reading frame. in cypovirus type 1 (cpv-1), a 62-codon aug-initiated ... | 2008 | 18492230 |
| genomic and biological characterization of a new cypovirus isolated from dendrolimus punctatus. | a novel cypovirus (designated dpcpv-mc) was isolated from the pine moth dendrolimus punctatus using serial in vivo cloning procedures. dpcpv-mc occurs in typical polyhedral occlusion bodies, containing a number of spherical virions. laboratory bioassays indicated that the infectivity of dpcpv-mc against second-instar spodoptera exigua larvae does not differ significantly from that of dendrolimus punctatus cypovirus 1. full-length amplification of the dpcpv-mc cdnas identified 16 dsrna genome seg ... | 2014 | 25419713 |
| cell proliferation by silk gut incorporating fgf-2 protein microcrystals. | silk gut processed from the silk glands of the silkworm could be an ideal biodegradable carrier for cell growth factors. we previously demonstrated that polyhedra, microcrystals of cypovirus 1 polyhedrin, can serve as versatile carrier proteins. here, we report the generation of a transgenic silkworm that expresses polyhedrin together with human basic fibroblast growth factor (fgf-2) in its posterior silk glands to utilize silk gut as a proteinaceous carrier to protect and slowly release active ... | 2015 | 26053044 |
| characterization of the complete genome segments from bmcpv-sz, a novel bombyx mori cypovirus 1 isolate. | a novel bombyx mori cypovirus 1 isolated from infected silkworm larvae and tentatively assigned as bombyx mori cypovirus 1 isolate suzhou (bmcpv-sz). the complete nucleotide sequences of genomic segments s1-s10 from bmcpv-sz were determined. all segments possessed a single open reading frame; however, bioinformatic evidence suggested a short overlapping coding sequence in s1. each bmcpv-sz segment possessed the conserved terminal sequences aguaa and guuagcc at the 5' and 3' ends, respectively. t ... | 2012 | 22712678 |
| transmission electron microscopy studies of cellular responses to entry of virions: one kind of natural nanobiomaterial. | virions are one kind of nanoscale pathogen and are able to infect living cells of animals, plants, and bacteria. the infection is an intrinsic property of the virions, and the biological process provides a good model for studying how these nanoparticles enter into cells. during the infection, the viruses employ different strategies to which the cells have developed respective responses. for this paper, we chose bombyx mori cypovirus 1 (bmcpv-1) interactions with midgut cells from silkworm, and s ... | 2012 | 22567012 |