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assessment of globodera pallida rna extracted from solanum roots.the introduction of high-throughput sequencing technologies has made transcriptome analyses of plant-pathogen interactions almost routine. nevertheless, it is still challenging to obtain rna from populations made up of two species. an rna extraction method that worked well on free-living caenorhabditis elegans failed when applied to isolated globodera pallida j2 larva. furthermore, alternative protocols that extracted rna from free-living j2 larva produced less satisfactory results once the anim ...201728512373
potential of tissue culture for breeding root-knot nematode resistance into vegetables.plant protoplast technology is being investigated as a means of transferring root-knot nematode resistance factors from solanum sisymbriifolium into the susceptible s. melongena. solanum sisymbriifolium plants regenerated from callus lost resistance to meloidogyne javanica but retained resistance to m. incognita. tomato plants cloned from leaf discs of the root-knot nematode resistant 'patriot' were completely susceptible to m. incognita, while sections of stems and leaves rooted in sand in the ...198219295668
evaluation of antileishmanial activity of selected brazilian plants and identification of the active principles.this study evaluated extracts, fractions, and isolated compounds from some selected brazilian medicinal plants against strains of promastigotes of leishmania amazonensis and l. brasiliensis in vitro. the cell viability was determined, comparing the results with reference standards. the dichloromethane fractions of the roots, stems, and leaves of allamanda schottii showed ic50 values between 14.0 and 2.0  μ g/ml. plumericin was the main active compound, with ic50 of 0.3 and 0.04  μ g/ml against t ...201323840252
cytotoxic effects of argentinean plant extracts on tumour and normal cell lines.in the search for possible new anti-cancer agents, we investigated the effects of 75 aqueous and methanol extracts from 41 argentinean plant species. the effect in cell growth was evaluated in the lm2 mammary adenocarcinoma cells. in a second stage, the highly active selected extracts were assayed in 3 other tumour cell lines: melanoma b16, bladder mb49 and lung a549; and 3 normal cell lines: mammary hb4a and keratinocytes pam212 and hacat. eight methanol extracts were found to be highly cytotox ...201121624335
antihypertensive effect of nuatigenin-3-o-ß-chacotriose from solanum sisymbriifolium lam. (solanaceae) (ñuatî pytâ) in experimentally hypertensive (arh+doca) rats under chronic administration.the aim of the study is to assess the hypotensive properties of the hydro-ethanolic crude root extract (cre), the n-butanol fraction (f(btoh)) and nuatigenin-3-o-ß-chacotriose, from solanum sisymbriifolium lam., in adrenal regeneration hypertension+deoxycorticosterone acetate (arh+doca) rats, following a chronic administration. the roots of s. sisymbriifolium lam. (solanaceae) were extracted by reflux with ethanol-water 7:3 and the active extract was fractionated by bioassay-guided liquid-liquid ...201121353509
anticonvulsant activity of solasodine isolated from solanum sisymbriifolium fruits in rodents.solanum sisymbriifolium lam. (solanaceae), commonly known as sticky nightshade, is traditionally used for central nervous system (cns) disorders. although solasodine has been isolated from this plant, little is known about its anticonvulsant and cns depressant actions.201021062107
isolation of hypotensive compounds from solanum sisymbriifolium lam.the crude hydroalcoholic root extract (cre) of solanum sisymbriifolium lam. has formerly been shown to have hypotensive activity both in normo-and hypertensive rats. hypotensive activity-guided fractionation of the cre was performed in anaesthetized normotensive rats, which led to the isolation of the active principles. the intravenous (i.v.) and intraperitoneal (i.p.) values of the cre in mice were found to be, respectively, 343 and 451 mg/kg, and no lethal effect was caused by doses up to 5.0 ...200010837991
hypotensive effect of crude root extract of solanum sisymbriifolium (solanaceae) in normo- and hypertensive rats.the hypotensive effect of the crude hydroalcoholic extract from root of solanum sisymbriifolium lam. (solanaceae) was investigated both in normotensive and hypertensive rats. the intravenous administration of the extract (50 and 100 mg/kg) produced a significant decrease in blood pressure in anaesthetized hypertensive (adrenal regeneration hypertension + deoxycorticosterone acetate (arh + doca)) rats. oral administration of the extract (10, 50, 100 and 250 mg/kg) also produced a dose-dependent h ...19968941862
role of mineral nutrients and growth regulators in the apical dominance in solanum sisymbrifolium.the lower axillary buds of intact plants of solanum sisymbrifolium are released from complete inhibition by supplying high doses of potassium to the soil while complete apical dominance is shown by plants grown at low, but not deficiency levels of k. nitrate and phosphate supplied alone or together are ineffective but when either of them is supplied with k, the effect of the latter on the growth of axillary buds is somewhat enhanced. the buds thus released from inhibition elongate further only w ...197024500046
solasodine levels in solanum sisymbriifolium lam.solasodine content in berries, leaves and stem of s. sisymbriifolium, using three different procedures, was 0.93, 0.73 and 0.23% respectively. further in chromatogram not only solasodine but three more alkaloids (rf 0.22, 0.48 and 0.88) were found which could not be identified due to lack of authentic samples.19892807409
a new steroidal saponin from solanum sisymbriifolium roots.isonuatigenin-3-o-beta-solatriose (1) was isolated from the roots of solanum sisymbriifolium. its structure was determined by spectroscopic methods.200515990250
on the nature and origin of the oxalate package in solanum sisymbriifolium anthers.this is a detailed study carried out in solanum sisymbriifolium lam. on the development of the circular cell cluster (ccc) during crystal deposition, as well as the composition of the crystals. light microscopy and scanning and transmission electron microscopy (tem) were used to characterize tissue throughout anther development. energy dispersive x-ray analysis (edax) allowed the determination of the elemental composition of crystals that form in the ccc region, and infrared and x-ray diffractio ...201020372946
evaluation of the biological activity of the molluscicidal fraction of solanum sisymbriifolium against non target organisms.the evaluation of the biocidal activity of the fruit of solanum sisymbriifolium involving non target organisms such as aquatic insects, fish and snails lead to the isolation of the steroidal alkaloids, solamargine and β-solamarine, from the active fractions. the fractions a3 and c, with biological activity against fish, snail and aquatic insect and larvae, are able to affect the good functioning of ecosystem found on alimentary chain. the fraction b seems to be less toxic to fish and aquatic ins ...201020388535
analgesic, neuropharmacological, anti-diarrheal, and cytotoxic activities of the extract of solanum sisymbriifolium (lam.) leaves.the present study was undertaken to evaluate the possible analgesic, neuropharmacological, anti-diarrheal, and cytotoxic activities of the ethanol extract of leaves of solanum sisymbriifolium lam. (family: solanaceae).201325050287
evidences of antihypertensive potential of extract from solanum capsicoides all. in spontaneously hypertensive rats.solanum capsicoides all. is morphologically similar to solanum sisymbriifolium lam. which is used in folk medicine in south america for antihypertensive and diuretics purposes. this similarity has led to species identification errors, which therefore may result in errors by patients.201627064009
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