| proposal of cobetia marina gen. nov., comb. nov., within the family halomonadaceae, to include the species halomonas marina. | at present the family halomonadaceae consists of four genera and the taxonomic status of all species included in these genera is not equally supported by phylogenetic evidence. whereas zymobacter and carnimonas contain one single species each and form the deeper branch of the family, the genus halomonas contains currently 22 species, with a maximum 16s/23s rrna sequence divergence greater than it could be expected for species of the same genus. in addition, we have witnessed during last years an ... | 2002 | 12353874 |
| temperature and nutrient availability control growth rate and fatty acid composition of facultatively psychrophilic cobetia marina strain l-2. | a facultative psychrophilic bacterium, strain l-2, that grows at 0 and 5 degrees c as minimum growth temperatures in complex and defined media, respectively, was isolated. on the basis of taxonomic studies, strain l-2 was identified as cobetia marina. the adaptability of strain l-2 to cold temperature was higher than that of the type strain and of other reported strains of the same species. when the bacterium was grown at 5-15 degrees c in a defined medium, it produced a high amount of trans-uns ... | 2004 | 15067498 |
| effect of substratum surface chemistry and surface energy on attachment of marine bacteria and algal spores. | two series of self-assembled monolayers (sams) of omega-substituted alkanethiolates on gold were used to systematically examine the effects of varying substratum surface chemistry and energy on the attachment of two model organisms of interest to the study of marine biofouling, the bacterium cobetia marina (formerly halomonas marina) and zoospores of the alga ulva linza (formerly enteromorpha linza). sams were formed on gold-coated glass slides from solutions containing mixtures of methyl- and c ... | 2004 | 15240295 |
| a highly active alkaline phosphatase from the marine bacterium cobetia. | an alkaline phosphatase with unusually high specific activity has been found to be produced by the marine bacterium cobetia marina (strain kmm mc-296) isolated from coelomic liquid of the mussel crenomytilus grayanus. the properties of enzyme, such as a very high specific activity (15000 de u/1 mg of protein), no activation with divalent cations, resistance to high concentrations of inorganic phosphorus, as well as substrate specificity toward 5' nucleotides suggest that the enzyme falls in an i ... | 2005 | 15906116 |
| halomonas taeanensis sp. nov., a novel moderately halophilic bacterium isolated from a solar saltern in korea. | a moderately halophilic, gram-negative bacterium, strain bh539(t), which was isolated from a solar saltern at taean in korea, was considered to be a member of the genus halomonas. strain bh539(t) grew at salinities of 1-25% (w/v) and at temperatures of 10-45 degrees c. cells were short rods that were motile by means of several flagella. their major fatty acids were c(18:1)omega7c, c(16:0) and c(19:0) cyclo omega8c. the genomic dna g+c content was about 65 mol% and the predominant ubiquinone was ... | 2005 | 16166705 |
| reversible protein adsorption and bioadhesion on monolayers terminated with mixtures of oligo(ethylene glycol) and methyl groups. | surface-grafted, environmentally responsive polymers have shown great promise for controlling adsorption and desorption of macromolecules and cells on solid surfaces. in the paper, we demonstrate that certain mixed self-assembled monolayers (sams) of oligo(ethylene glycol) (oeg) and methyl-terminated alkanethiolates on gold form surfaces with switchable hydrophobicity and tendency for protein adsorption and cellular attachment. at temperatures above 32 degrees c, sams with a surface density of a ... | 2005 | 16231888 |
| chemical defence in mussels: antifouling effect of crude extracts of the periostracum of the blue mussel mytilus edulis. | shells of the blue mussel mytilus edulis remain free of fouling organisms as long as they possess an intact periostracum, and a multiple antifouling defence that comprises a ripple-like microtopography and the production of chemical antifouling compounds has been suggested previously. this study investigates the chemical defence strategy of blue mussels for the first time. six crude extracts of the periostracum of intact shells were made using solvents of increasing polarity. these extracts were ... | 2006 | 17290869 |
| combinatorial materials research applied to the development of new surface coatings iii. utilisation of a high-throughput multiwell plate screening method to rapidly assess bacterial biofilm retention on antifouling surfaces. | the authors recently reported on the development of a novel multiwell plate screening method for the high-throughput assessment of bacterial biofilm retention on surfaces. two series of biocide containing coatings were prepared to assess the ability of the developed assay to adequately discern differences in antifouling performance: i) a commercially available poly(methyl methacrylate) (pmma) and silicone elastomer (dc) physically blended with an organic antifouling biocide sea-nine 211 (sn211) ... | 2007 | 17453727 |
| evaluation of the activity of the sponge metabolites avarol and avarone and their synthetic derivatives against fouling micro- and macroorganisms. | the sesquiterpene hydroquinone avarol (1) was isolated from the marine sponge dysidea avara, whereas the corresponding quinone, avarone (2), was obtained by oxidation of avarol, and the significantly more lipophilic compounds [3'-(p-chloro-phenyl)avarone (3), 3',4'-ethylenedithioavarone (4), 4'-isopropylthioavarone (5), 4'-tert-butylthioavarone (6), 4'-propylthioavarone (7), 4'-octylthioavarone (8)] were obtained by nucleophilic addition of thiols or p-chloroaniline to avarone. all these compoun ... | 2007 | 17873837 |
| modicisalibacter tunisiensis gen. nov., sp. nov., an aerobic, moderately halophilic bacterium isolated from an oilfield-water injection sample, and emended description of the family halomonadaceae franzmann et al. 1989 emend dobson and franzmann 1996 emend. ntougias et al. 2007. | an aerobic, moderately halophilic, gram-negative, motile, non-sporulating bacterium, strain lit2(t), was isolated from an oilfield-water injection after enrichment on crude oil. strain lit2(t) grew between 15 and 45 degrees c and optimally at 37 degrees c. it grew in the presence of 1-25 % (w/v) nacl, with an optimum at 10 % (w/v) nacl. predominant fatty acids were c(16 : 0) (26.9 %), c(18 : 1)omega7c (22.6 %), c(16 : 1)omega7c (20.4 %) c(19 : 0) cyclo omega8c (10.9 %) and c(17 : 0) (8 %). inter ... | 2007 | 17911302 |
| light-induced biocidal action of conjugated polyelectrolytes supported on colloids. | a series of water soluble, cationic conjugated polyelectrolytes (cpes) with backbones based on a poly(phenylene ethynylene) repeat unit structure and tetraakylammonium side groups exhibit a profound light-induced biocidal effect. the present study examines the biocidal activity of the cpes, correlating this activity with the photophysical properties of the polymers. the photophysical properties of the cpes are studied in solution, and the results demonstrate that direct excitation produces a tri ... | 2008 | 18729335 |
| poly(ethylene glycol)-containing hydrogel surfaces for antifouling applications in marine and freshwater environments. | this work describes the fabrication, characterization, and biological evaluation of a thin protein-resistant poly(ethylene glycol) (peg)-based hydrogel coating for antifouling applications. the coating was fabricated by free-radical polymerization on silanized glass and silicon and on polystyrene-covered silicon and gold. the physicochemical properties of the coating were characterized by infrared spectroscopy, ellipsometry, and contact angle measurements. in particular, the chemical stability o ... | 2008 | 18759475 |
| isolation and characterization of biosurfactant-producing alcanivorax strains: hydrocarbon accession strategies and alkane hydroxylase gene analysis. | biosurfactant-producing bacteria belonging to the genera alcanivorax, cobetia and halomonas were isolated from marine sediments with a history of hydrocarbon exposure (aristizábal and gravina peninsulas, argentina). two alcanivorax isolates were found to form naturally occurring consortia with strains closely related to pseudomonas putida and microbacterium esteraromaticum. alkane hydroxylase gene analysis in these two alcanivorax strains resulted in the identification of two novel alkb genes, s ... | 2009 | 18983915 |
| cobetia crustatorum sp. nov., a novel slightly halophilic bacterium isolated from traditional fermented seafood in korea. | a slightly halophilic, gram-stain-negative, straight-rod-shaped aerobe, strain jo1(t), was isolated from jeotgal, a traditional korean fermented seafood. cells were observed singly or in pairs and had 2-5 peritrichous flagella. optimal growth occurred at 25 degrees c, in 6.5 % (w/v) salts and at ph 5.0-6.0. strain jo1(t) was oxidase-negative and catalase-positive. cells did not reduce fumarate, nitrate or nitrite on respiration. acid was produced from several carbohydrates and the strain utilize ... | 2010 | 19654339 |
| phylogenetic relationships within the family halomonadaceae based on comparative 23s and 16s rrna gene sequence analysis. | a phylogenetic study of the family halomonadaceae was carried out based on complete 16s rrna and 23s rrna gene sequences. several 16s rrna genes of type strains were resequenced, and 28 new sequences of the 23s rrna gene were obtained. currently, the family includes nine genera (carnimonas, chromohalobacter, cobetia, halomonas, halotalea, kushneria, modicisalibacter, salinicola and zymobacter). these genera are phylogenetically coherent except halomonas, which is polyphyletic. this genus compris ... | 2010 | 19656941 |
| bacterial assay for the rapid assessment of antifouling and fouling release properties of coatings and materials. | an assay has been developed to accurately quantify the growth and release behaviour of bacterial biofilms on several test reference materials and coatings, using the marine bacterium cobetia marina as a model organism. the assay can be used to investigate the inhibition of bacterial growth and release properties of many surfaces when compared to a reference. the method is based upon the staining of attached bacterial cells with the nucleic acid-binding, green fluorescent syto 13 stain. a strong ... | 2010 | 20039190 |
| [hydroxyectoine synthesis and release under osmotic shock in cobetia marina cicc10367]. | to obtain hydroxyectoine-producing strain with tolerance to osmotic shock and improve hydroxyectoine productivity by adopting "bacteria milking" process. | 2009 | 20222443 |
| conjugated polyelectrolyte capsules: light-activated antimicrobial micro "roach motels". | microcapsules consisting of alternating layers of oppositely charged poly(phenylene ethynylene)-type conjugated polyelectrolytes (cpes) were prepared via layer-by-layer deposition onto mnco3 template particles followed by dissolution of the template particles using an ethylenediaminetetraacetate solution. the resulting microcapsules exhibit bright-green fluorescence emission characteristics of the cpes. strong antimicrobial activity was observed upon mixing of polyelectrolyte capsules with cobet ... | 2009 | 20355752 |
| poly(ethylene glycol)-based thiol-ene hydrogel coatings-curing chemistry, aqueous stability, and potential marine antifouling applications. | photocured thiol-ene hydrogel coatings based on poly(ethylene glycol) (peg) were investigated for marine antifouling purposes. by varying the peg length, vinylic end-group, and thiol cross-linker, a library of hydrogel coatings with different structural composition was efficiently accomplished, with or without ester linkages. the thiol-methacrylate and thiol-allyl systems were evaluated with respect to curing, degradation, as well as antifouling properties. methacrylate-based systems exhibited h ... | 2010 | 20356297 |
| identities of epilithic hydrocarbon-utilizing diazotrophic bacteria from the arabian gulf coasts, and their potential for oil bioremediation without nitrogen supplementation. | gravel particles from four sites along the arabian gulf coast in autumn, winter, and spring were naturally colonized with microbial consortia containing between 7 and 400 × 10(2) cm(-2) of cultivable oil-utilizing bacteria. the 16s rrna gene sequences of 70 representatives of oil-utilizing bacteria revealed that they were predominantly affiliated with the gammaproteobacteria and the actinobacteria. the gammaproteobacteria comprised among others, the genera pseudomonas, pseudoalteromonas, shewane ... | 2010 | 20567813 |
| diversity of the predominant spoilage bacteria in water-boiled salted duck during storage. | the spoilage microbiota in water-boiled salted duck during storage at 4 degrees c was determined using culture-dependent and independent methods. analysis of the denaturing gradient gel electrophoresis (dgge) patterns of pcr amplicons targeting the v3 region of the 16s rdna and sequencing of the bands allowed profiling of the microbiota present in the duck. community dna extracts were prepared directly from water-boiled salted duck and from culturable bacterial fractions harvested from both mrs ... | 2010 | 20629890 |
| engineered antifouling microtopographies: the role of reynolds number in a model that predicts attachment of zoospores of ulva and cells of cobetia marina. | a correlation between the attachment density of cells from two phylogenetic groups (prokaryotic bacteria and eukaryotic plantae), with surface roughness is reported for the first time. the results represent a paradigm shift in the understanding of cell attachment, which is a critical step in the biofouling process. the model predicts that the attachment densities of zoospores of the green alga, ulva, and cells of the marine bacterium, cobetia marina, scale inversely with surface roughness. the s ... | 2010 | 20706891 |
| the cq ratio of surface energy components influences adhesion and removal of fouling bacteria. | the interaction energy between bacteria and substrata with different surface energies was modelled by the extended dlvo-á(derjaguin, landau, verwey and overbeek) theory. the modeling results revealed that the interaction energy has a strong correlation with the cq (chen and qi) ratio, which is defined as the ratio of the lifshitz-van der waals (lw) apolar to the electron donor surface energy components of substrata. both modeling and experimental results with different bacteria including p. fluo ... | 2011 | 21390912 |
| antifouling performance of cross-linked hydrogels: refinement of an attachment model. | poly(ethylene glycol) dimethacrylate (pegdma), pegdma-co-glycidyl methacrylate (pegdma-co-gma), and pegdma-co-hydroxyethyl methacrylate (pegdma-co-hema) hydrogels were polymerized using ammonium persulfate and ascorbic acid as radical initiators. surface energies of the hydrogels and a standard, poly(dimethylsiloxane) elastomer (pdmse), were characterized using captive bubble and sessile drop measurements, respectively (? = 52 mn/m, ?(0) = 19 mn/m). the chemical composition of the hydrogels was ... | 2011 | 21401017 |
| x-ray holographic microscopy with zone plates applied to biological samples in the water window using 3rd harmonic radiation from the free-electron laser flash. | the imaging of hydrated biological samples - especially in the energy window of 284-540 ev, where water does not obscure the signal of soft organic matter and biologically relevant elements - is of tremendous interest for life sciences. free-electron lasers can provide highly intense and coherent pulses, which allow single pulse imaging to overcome resolution limits set by radiation damage. one current challenge is to match both the desired energy and the intensity of the light source. we presen ... | 2011 | 21716334 |
| resistance of galactoside-terminated alkanethiol self-assembled monolayers to marine fouling organisms. | self-assembled monolayers (sams) of galactoside-terminated alkanethiols have protein-resistance properties which can be tuned via the degree of methylation [langmuir 2005, 21, 2971-2980]. specifically, a partially methylated compound was more resistant to nonspecific protein adsorption than the hydroxylated or fully methylated counterparts. we investigate whether this also holds true for resistance to the attachment and adhesion of a range of marine species, in order to clarify to what extent re ... | 2011 | 21916438 |
| cloning and expression of a highly active recombinant alkaline phosphatase from psychrotrophic cobetia marina. | alkaline phosphatase catalyzes the hydrolysis of phosphomonoesters and is widely used in molecular biology techniques and clinical diagnostics. we expressed a recombinant alkaline phosphatase of the marine bacterium, cobetia marina, in escherichia coli bl21 (de3). the recombinant protein was purified with a specific activity of 12,700 u/mg protein, which is the highest activity reported of any bacterial alkaline phosphatase studied to date. the molecular mass of the recombinant protein was 55-60 ... | 2011 | 22009571 |
| Isolation and characterization of crude-oil-degrading bacteria from the Persian Gulf and the Caspian Sea. | Twenty-five crude-oil-degrading bacteria were isolated from oil-contaminated sites in the Persian Gulf and the Caspian Sea. Based on a high growth rate on crude oil and on hydrocarbon degradation ability, 11 strains were selected from the 25 isolated strains for further study. Determination of the nucleotide sequence of the 16S rRNA gene showed that these isolated strains belonged to genera Acinetobacter, Pseudomonas, Gordonia, Rhodococcus, Cobetia, Halomonas, Alcanivorax, Marinobacter and Micro ... | 2012 | 22130193 |
| An extended suite of genetic tools for use in bacteria of the halomonadaceae: an overview. | Halophilic gammaproteobacteria of the family Halomonadaceae (including the genera Aidingimonas, Carnimonas, Chromohalobacter, Cobetia, Halomonas, Halotalea, Kushneria, Modicisalibacter, Salinicola, and Zymobacter) have current and promising applications in biotechnology mainly as a source of compatible solutes (powerful stabilizers of biomolecules and cells, with exciting potentialities in biomedicine), salt-tolerant enzymes, biosurfactants, and extracellular polysaccharides, among other product ... | 2012 | 22160899 |