Publications

functional analysis of the lysis genes of staphylococcus aureus phage p68 in escherichia coli.	double-stranded dna phages of both gram-positive and gram-negative bacteria typically use a holin-endolysin system to achieve lysis of their host. in this study, the lysis genes of staphylococcus aureus phage p68 were characterized. p68 gene lys16 was shown to encode a cell-wall-degrading enzyme, which causes cell lysis when externally added to clinical isolates of s. aureus. another gene, hol15, was identified embedded in the -1 reading frame at the 3' end of lys16. the deduced hol15 protein ha ...	2005	16000723
antimicrobial activity of a chimeric enzybiotic towards staphylococcus aureus.	phage lytic enzymes (enzybiotics) have gained attention as prospective tools to eradicate gram-positive pathogens resistant to antibiotics. attempts to purify the p16 endolysin of staphylococcus aureus phage p68 were unsuccessful owing to the poor solubility of the protein. to overcome this limitation, we constructed a chimeric endolysin (p16-17) comprised of the inferred n-terminal d-alanyl-glycyl endopeptidase domain and the c-terminal cell wall targeting domain of the s. aureus phage p16 endo ...	2009	18940209