| transfer of pectobacterium chrysanthemi (burkholder et al. 1953) brenner et al. 1973 and brenneria paradisiaca to the genus dickeya gen. nov. as dickeya chrysanthemi comb. nov. and dickeya paradisiaca comb. nov. and delineation of four novel species, dickeya dadantii sp. nov., dickeya dianthicola sp. nov., dickeya dieffenbachiae sp. nov. and dickeya zeae sp. nov. | a collection of 75 strains of pectobacterium chrysanthemi (including all biovars and pathovars) and the type strains of brenneria paradisiaca (cfbp 4178(t)) and pectobacterium cypripedii (cfbp 3613(t)) were studied by dna-dna hybridization, numerical taxonomy of 121 phenotypic characteristics, serology and 16s rrna gene-based phylogenetic analyses. from analysis of 16s rrna gene sequences, it was deduced that p. chrysanthemi strains and b. paradisiaca cfbp 4178(t) formed a clade distinct from th ... | 2005 | 16014461 |
| potential of bulb-associated bacteria for biocontrol of hyacinth soft rot caused by dickeya zeae. | dickeya zeae is a pectinolytic bacterium responsible for soft rot disease in flower bulb crops. in this study, the possibility of controlling soft rot disease in hyacinth by using antagonistic bacteria isolated from hyacinth bulbs was explored. | 2009 | 19054227 |
| 13c labeling reveals multiple amination reactions in the biosynthesis of a novel polyketide polyamine antibiotic zeamine from dickeya zeae. | the structure of zeamine, a novel polyamino-amide antibiotic metabolite of dickeya zeae has been established by nmr and detailed ms analyses; labelling studies with (13)c-labelled acetates suggest that the repeating secondary amine containing motif may be biosynthesised via a modular pks containing aminotransferase domains. | 2010 | 20024369 |
| rice root-associated bacteria: insights into community structures across 10 cultivars. | in this study, the effects of plant genotype, soil type and nutrient use efficiency on the composition of different bacterial communities associated with rice roots were investigated. thus, total bacteria, alpha- and betaproteobacteria, pseudomonas and actinobacteria were studied using pcr, followed by denaturing gradient gel electrophoresis (pcr-dgge). rice genotype determined, to a large extent, the composition of the different bacterial communities across cultivars. several cultivars belongin ... | 2011 | 21426364 |
| a novel multidomain polyketide synthase is essential for zeamine production and the virulence of dickeya zeae. | dickeya zeae is the causal agent of the rice foot rot disease, but its mechanism of infection remains largely unknown. in this study, we identified and characterized a novel gene designated as zmsa. the gene encodes a large protein of 2,346 amino acids in length, which consists of multidomains arranged in the order of n-terminus, β-ketoacyl synthase, acyl transferase, acyl carrier protein, β-ketoacyl reductase, dehydratase. this multidomain structure and sequence alignment analysis suggest that ... | 2011 | 21899437 |
| production of novel antibiotics zeamines through optimizing dickeya zeae fermentation conditions. | dickeya zeae strain ec1 was recently shown to produce a new type of phytotoxins designated as zeamine and zeamine ii, which are potent wide-spectrum antibiotics against gram-positive and gram-negative bacterial pathogens, suggesting their promising potential as clinical medicines. in this study, the optimized medium composition and culture conditions for biosynthesis of novel antibiotics zeamines have been established by using response surface methodology, largely increasing the yield of zeamine ... | 2014 | 25541733 |
| genetic modulation of c-di-gmp turnover affects multiple virulence traits and bacterial virulence in rice pathogen dickeya zeae. | the frequent outbreaks of rice foot rot disease caused by dickeya zeae have become a significant concern in rice planting regions and countries, but the regulatory mechanisms that govern the virulence of this important pathogen remain vague. given that the second messenger cyclic di-gmp (c-di-gmp) is associated with modulation of various virulence-related traits in various microorganisms, here we set to investigate the role of the genes encoding c-di-gmp metabolism in the regulation of the bacte ... | 2016 | 27855163 |
| control of litchi downy blight by zeamines produced by dickeya zeae. | zeamines (zms), a class of polyamine-polyketide-nonribosomal peptide produced by bacterial isolate dickeya zeae, were shown recently to be potent antibiotics against some bacterial pathogens. in this study, the results indicated that zms showed antifungal activity against peronophythora litchii and other fungal pathogens. the activity of zms against the oomycete pathogen p. litchi, which causes the devastating litchi downy blight, was further investigated under in vitro and in vivo conditions. z ... | 2015 | 26499339 |
| the complete genome sequence of dickeya zeae ec1 reveals substantial divergence from other dickeya strains and species. | dickeya zeae is a bacterial species that infects monocotyledons and dicotyledons. two antibiotic-like phytotoxins named zeamine and zeamine ii were reported to play an important role in rice seed germination, and two genes associated with zeamines production, i.e., zmsa and zmsk, have been thoroughly characterized. however, other virulence factors and its molecular mechanisms of host specificity and pathogenesis are hardly known. | 2015 | 26239726 |
| genome sequence of the rice pathogen dickeya zeae strain zju1202. | dickeya zeae is a phytopathogenic bacterium causing soft rot diseases in a wide range of economically important crops. here we present the draft genome sequence of strain zju1202, which is the causal agent of rice foot rot in china. the draft genome will contribute to epidemiological and comparative genomic studies and the quarantine of this devastating phytopathogen. | 2012 | 22843587 |
| draft genome sequence of the plant pathogen dickeya zeae dz2q, isolated from rice in italy. | dickeya zeae is an emerging rice (oryza sativa) pathogen causing bacterial foot rot. related pathogens affect maize (zea mays) and potato (solanum tuberosum) and a variety of important ornamental and floral plants. here, we present the draft genome sequence of d. zeae dz2q, an isolate obtained from rice grown in italy. | 2013 | 24201194 |
| growth characteristics and biofilm formation of various spoilage bacteria isolated from fresh produce. | this study investigated the characteristics of spoilage bacteria isolated from fresh produce including growth at various temperatures, biofilm formation, cell hydrophobicity, and colony spreading. the number of spoilage bacteria present when stored at 35 °c was significantly greater than when stored at lower temperatures, and maximum population size was achieved after 10 h. however, bacillus pumilus, dickeya zeae, pectobacterium carotovorum subsp. carotovorum pcc21, and bacillus pumilus (rda-r) ... | 2014 | 25224561 |
| six new acylphloroglucinols from dryopteris championii. | six new acylphloroglucinols (1-6) were isolated from dryopteris championii. their structures were established on the basis of extensive analysis of spectroscopic data and comparison with reported data. the antibacterial activities of the isolates were evaluated against staphylococcus aureus, escherichia coli, bacillus subtilis and dickeya zeae. this article is protected by copyright. all rights reserved. | 2017 | 28323385 |
| six new pentacyclic triterpenoids from the fruit of camptotheca acuminata. | six new pentacyclic triterpenoids were isolated from the fruit of camptotheca acuminata. the chemical structures of the new compounds were elucidated by extensive spectroscopic analysis including hr-esi-ms, ir, uv, 1d- and 2d-nmr. moreover, the antibacterial activities of compounds 1, 2, 4, 5, and 6 were evaluated against staphylococcus aureus, escherichia coli, bacillus subtilis and dickeya zeae. all these tested compounds showed moderate antibacterial activity against bacillus subtilis and dic ... | 2017 | 27584719 |
| characterization of dickeya and pectobacterium species by capillary electrophoretic techniques and maldi-tof ms. | dickeya and pectobacterium species represent an important group of broad-host-range phytopathogens responsible for blackleg and soft rot diseases on numerous plants including many economically important plants. although these species are commonly detected using cultural, serological, and molecular methods, these methods are sometimes insufficient to classify the bacteria correctly. on that account, this study was undertaken to investigate the feasibility of three individual analytical techniques ... | 2015 | 26229029 |
| slya regulates phytotoxin production and virulence in dickeya zeae ec1. | dickeya zeae is a causal agent of rice root rot disease. the pathogen is known to produce a range of virulence factors, including phytotoxic zeamines and extracellular enzymes, but the mechanisms of virulence regulation remain vague. in this study, we identified a slya/marr family transcription factor slya in d. zeae strain ec1. disruption of slya significantly decreased zeamine production, enhanced swimming and swarming motility, reduced biofilm formation and significantly decreased pathogenici ... | 2016 | 26814706 |
| functional characterization of the sucrose isomerase responsible for trehalulose production in plant-associated pectobacterium species. | fifty-three plant-associated microorganisms were investigated for their ability to convert sucrose to its isomers. these microorganisms included one dickeya zeae isolate and 7 enterobacter, 3 pantoea, and 43 pectobacterium species. eleven out of the 53 strains (21%) showed the ability to transform sucrose to isomaltulose and trehalulose. among those, pectobacterium carotovorum kkh 3-1 showed the highest bioconversion yield (97.4%) from sucrose to its isomers. in this strain, the addition of up t ... | 2014 | 24411451 |
| a nonribosomal peptide synthase containing a stand-alone condensation domain is essential for phytotoxin zeamine biosynthesis. | dickeya zeae is the causal agent of rice foot rot and maize stalk rot diseases, which could cause severe economic losses. the pathogen is known to produce two phytotoxins known as zeamine and zeamine ii which are also potent antibiotics against both gram-positive and gram-negative bacteria pathogens. zeamine ii is a long-chain aminated polyketide and zeamine shares the same polyketide structure as zeamine ii, with an extra valine derivative moiety conjugated to the primary amino group of zeamine ... | 2013 | 23883359 |
| genome sequence of the banana pathogen dickeya zeae strain ms1, which causes bacterial soft rot. | we report a draft genome sequence of dickeya zeae strain ms1, which is the causative agent of banana soft rot in china, and we show several of its specific properties compared with those of other d. zeae strains. genome sequencing provides a tool for understanding the genomic determination of the pathogenicity and phylogeny placement of this pathogen. | 2013 | 23766402 |
| comparative analysis of two members of the metal ion-containing group iii-alcohol dehydrogenases from dickeya zeae. | a pair of nad(+)- and nadp(+)-dependent group iii-alcohol dehydrogenases was characterized from the enterobacterium, dickeya zeae, to expand our understanding of the distribution and biochemical properties of this interesting group of enzymes. two putative group iii-alcohol dehydrogenases (adhs) were identified in the genome of dickeya zeae. amino acid alignments and phylogenetic analysis revealed that adh3.1 and adh3.2 are only distantly related (~25 % identity at the protein level). both prote ... | 2013 | 23362047 |