Publications

TitleAbstractYear
Filter
PMID
Filter
infection by hemileia vastatrix. 19676053419
sensitization to the fungus hemileia vastatrix (coffee leaf rust).the aim of the present study was to determine the existence of sensitization to the fungus hemileia vastatrix (coffee leaf rust) in a sample of the brazilian population. this fungus attacks coffee plantations, and in some regions of brazil the concentration of its spores in the air can be very high.200111421929
impact of the coffea canephora gene introgression on beverage quality of c. arabica.lines of coffea arabica derived from the timor hybrid (hybrid between c. arabica and c. canephora) are resistant to coffee leaf rust (hemileia vastatrix) and to the nematode meloidogyne exigua. the introgression of c. canephora resistance genes is suspected of causing a drop in beverage quality. coffee samples from pure lines, compared in a trial 1, and from f1 hybrids and parental lines from a half-diallel trial in a trial 2, were studied for beverage quality, chemical composition and amount of ...200312750771
introgression molecular analysis of a leaf rust resistance gene from coffea liberica into c. arabica l.leaf rust caused by the fungus hemileia vastatrix is the most devastating disease of arabica coffee ( coffea arabica). therefore, developing leaf rust-resistant varieties has been a breeding objective of the highest priority in many countries. the purpose of the present work was to gain insight into the mechanism of introgression into c. arabica of a leaf rust resistance gene from c. liberica (i.e. s(h)3 resistance factor) and to identify associated molecular markers. an f(2) progeny (i.e. 101 i ...200415241596
farmers' perceptions, knowledge, and management of coffee pests and diseases and their natural enemies in chiapas, mexico.small farmers' perceptions of coffee coffea arabica l. herbivores and their natural enemies, how those perceptions relate to field infestation levels, and pest management practices being implemented by members from two organic and nonorganic coffee grower organizations in the soconusco region, southeastern mexico, were analyzed through an interview survey, diagnostic workshops, and field sampling. the terms pest, disease, and damage were commonly used as synonyms. the major phytophagous species, ...200415568334
polyphenoloxidase activity in coffee leaves and its role in resistance against the coffee leaf miner and coffee leaf rust.in plants, ppo has been related to defense mechanism against pathogens and insects and this role was investigated in coffee trees regarding resistance against a leaf miner and coffee leaf rust disease. ppo activity was evaluated in different genotypes and in relation to methyl-jasmonate (meja) treatment and mechanical damage. evaluations were also performed using compatible and incompatible interactions of coffee with the fungus hemileia vastatrix (causal agent of the leaf orange rust disease) a ...200616376392
genetic diversity in hemileia vastatrix based on rapd markers.random amplified polymorphic dna (rapd) was used to assess the genetic structure of hemileia vastatrix populations. forty-five rust isolates with different virulence spectra and from different hosts and geographical regions were analyzed. out of 45 bands, generated with three rapd primers, 35 (78%) were polymorphic and scored as molecular markers. cluster analysis exhibits unstructured variability of this pathogen with regard to physiological race, geographical origin or host. the genotypic dive ...200516396347
cytochrome b gene structure and consequences for resistance to qo inhibitor fungicides in plant pathogens.the cytochrome b (cyt b) gene structure was characterized for different agronomically important plant pathogens, such as puccinia recondita f sp tritici (erikss) co johnston, p graminis f sp tritici erikss and hennings, p striiformis f sp tritici erikss, p coronata f sp avenae p syd & syd, p hordei gh otth, p recondita f sp secalis roberge, p sorghi schwein, p horiana henn, uromyces appendiculatus (pers) unger, phakopsora pachyrhizi syd & p syd, hemileia vastatrix berk & broome, alternaria solan ...200616688790
characterizing plasma membrane h+-atpase in two varieties of coffee leaf (coffea arabica l.) and its interaction with an elicitor fraction from the orange rust fungus (h. vastatrix berk and br.) race ii.early intercellular signaling in coffea arabica l.-hemileia vastatrix host-pathogen interaction was studied, using inside-out plasma membrane from two varieties of coffee leaf and a fungal fraction to determine the plant's biochemical responses. microsomal pellets (100,000 x g) from the susceptible (caturra) and resistant (colombia) coffee leaf varieties were purified by partitioning in two-polymer dex (6.3% w/w) and peg (6.3% w/w) system aqueous phase. fungal material was obtained from orange r ...200616781870
transferability of ssr markers from related uredinales species to the coffee rust hemileia vastatrix.the aim of the present research was to test the transferability of simple sequence repeat (ssr) markers developed in two uredinales species to hemileia vastatrix, coffee rust. the development of efficient techniques for the identification of h. vastatrix isolates is imperative, given the continuous development of new races. the transferability of 25 ssr markers developed in the related uredinales species puccinia coronata f. sp lolli and melampsora linii to h. vastatrix was tested. a low level o ...200819048497
coffee (coffea arabica l.) genes early expressed during infection by the rust fungus (hemileia vastatrix).summary the beverage cash crop coffee (coffea arabica l.) is subject to severe losses caused by the rust fungus hemileia vastatrix. in naturally resistant coffee plants, a specific hypersensitive reaction (hr) may be elicited early to stop fungal infection. to isolate host genes involved in hr, we undertook an expressed sequence tags (ests) analysis. two cdna libraries were constructed using suppression subtractive hybridization (ssh) and 527 non-redundant ests were generated from 784 randomly p ...200420565627
454-pyrosequencing of coffea arabica leaves infected by the rust fungus hemileia vastatrix reveals in planta-expressed pathogen-secreted proteins and plant functions in a late compatible plant-rust interaction.coffee (coffea arabica l.), one of the key export and cash crops in tropical and subtropical countries, suffers severe losses from the rust fungus hemileia vastatrix. the transcriptome of h. vastatrix was analysed during a compatible interaction with coffee to obtain an exhaustive repertoire of the genes expressed during infection and to identify potential effector genes. large-scale sequencing (454-gs-flex titanium) of mixed coffee and rust cdnas obtained from 21-day rust-infected leaves genera ...201121726390
Validation of RT-qPCR reference genes for in planta expression studies in Hemileia vastatrix, the causal agent of coffee leaf rust.Hemileia vastatrix is a biotrophic fungus, causing coffee leaf rust in all coffee growing countries, leading to serious social and economic problems. Gene expression studies may have a key role unravelling the transcriptomics of this pathogen during interaction with the plant host. Reverse transcription quantitative real-time polymerase chain reaction (RT-qPCR) is currently the golden standard for gene expression analysis, although an accurate normalisation is essential for adequate conclusions. ...201121872186
Identification and characterization of the Non-race specific Disease Resistance 1 (NDR1) orthologous protein in coffee.Leaf rust, which is caused by the fungus Hemileia vastatrix (Pucciniales), is a devastating disease that affects coffee plants (Coffea arabica L.). Disadvantages that are associated with currently developed phytoprotection approaches have recently led to the search for alternative strategies. These include genetic manipulations that constitutively activate disease resistance signaling pathways. However, molecular actors of such pathways still remain unknown in C. arabica. In this study, we have ...201122023696
Cryptosexuality and the genetic diversity paradox in coffee rust, Hemileia vastatrix.Despite the fact that coffee rust was first investigated scientifically more than a century ago, and that the disease is one of the major constraints to coffee production--constantly changing the socio-economic and historical landscape of the crop--critical aspects of the life cycle of the pathogen, Hemileia vastatrix, remain unclear. The asexual urediniospores are regarded as the only functional propagule: theoretically, making H. vastatrix a clonal species. However, the well-documented emergen ...201122102860
Displaying items 1 - 15 of 15