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draft genome sequence of "candidatus phytoplasma oryzae" strain mbita1, the causative agent of napier grass stunt disease in kenya.phytoplasmas are bacterial plant pathogens with devastating impact on agricultural production worldwide. in eastern africa, napier grass stunt disease causes serious economic losses in the smallholder dairy industry. this draft genome sequence of " italic! candidatusphytoplasma oryzae" strain mbita1 provides insight into its genomic organization and the molecular basis of pathogenicity.201627103722
development of field-applicable tests for rapid and sensitive detection of candidatus phytoplasma oryzae.napier grass stunt disease (nsd) is a severe disease of napier grass (pennisetum purpureum) in eastern africa, caused by the leafhopper-transmitted bacterium candidatus phytoplasma oryzae. the pathogen severely impairs the growth of napier grass, the major fodder for dairy cattle in eastern africa. nsd is associated with biomass losses of up to 70% of infected plants. diagnosis of nsd is done by nested pcr targeting the phytoplasma dna, which is difficult to perform in developing countries with ...201728647581
diversity of bacterial endosymbionts associated with macrosteles leafhoppers vectoring phytopathogenic phytoplasmas.here, we investigate the endosymbiotic microbiota of the macrosteles leafhoppers m. striifrons and m. sexnotatus, known as vectors of phytopathogenic phytoplasmas. pcr, cloning, sequencing, and phylogenetic analyses of bacterial 16s rrna genes identified two obligate endosymbionts, "candidatus sulcia muelleri" and "candidatus nasuia deltocephalinicola," and five facultative endosymbionts, wolbachia, rickettsia, burkholderia, diplorickettsia, and a novel bacterium belonging to the rickettsiaceae, ...201323770905
"candidatus phytoplasma oryzae", a novel phytoplasma taxon associated with rice yellow dwarf disease.in addition to rice yellow dwarf (ryd) phytoplasma, several phytoplasmas infect gramineous plants, including rice orange leaf, bermuda grass white leaf, brachiaria grass white leaf and sugarcane white leaf phytoplasmas. to investigate whether the ryd phytoplasma is a discrete, species-level taxon, several isolates of the aforementioned phytoplasmas were analysed using pcr-amplified 16s rdna sequences. two ryd isolates, ryd-j(t) and ryd-th, were almost identical (99.2 %), but were distinct (simil ...200314657124
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