| characterization of a novel halophilic archaeon, halobiforma haloterrestris gen. nov., sp. nov., and transfer of natronobacterium nitratireducens to halobiforma nitratireducens comb. nov. | strain 135(t), a novel red-pigmented, aerobic, extremely halophilic member of the archaea showing rod, coccus and slightly pleomorphic morphology, was isolated from hypersaline soil close to aswan (egypt). this organism is neutrophilic, motile and requires at least 2.2 m nacl, but no mgcl2, for growth and exhibits optimal growth at 42 degrees c. polar lipid analysis revealed the presence of sulfated triglycosyl diether and triglycosyl diether as the sole glycolipids as well as the absence of the ... | 2002 | 12508897 |
| halobiforma lacisalsi sp. nov., isolated from a salt lake in china. | a gram-negative, motile, neutrophilic and extremely halophilic strain, aj5(t), was isolated from a salt lake in xinjiang, china, and subjected to polyphasic taxonomic study. the major polar lipids of the isolate were c(20)c(20) and c(20)c(25) derivatives of phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester and glycolipid. the dna g+c content was 64.9 mol%. phylogenetic analysis based on 16s rrna gene sequences indicated that strain aj5(t) clustered with members of the genus halob ... | 2005 | 16166693 |
| [diversity of halophilic archaea in hypersaline lakes of inner mongolia, china]. | the aims of this work were to explore the diversity of halophilic archaea in hypersaline lakes of inner mongolia, china and to collect novel halophilic archaea. one hundred and sixty-five halophilic archaea were isolated from the three different types of hypersaline lakes (erliannor, shangmatala and xilin soda lake) in inner mongolia. by analysis of the restriction patterns of amplified 16s rdna (ardra) with the enzyme afa i and hae ii, respectively, the isolates were clustered into 14 genotypes ... | 2006 | 16579455 |
| [biodiversity of halophilic archaea isolated from two salt lakes in xin-jiang region of china]. | there are more than 1000 salt lakes situated in northern and western regions of china and 790 of these salt lakes are in xinjiang uigur autonomous region and qinghai, tibet, inner mongolia. to better understand halophilic archaeal diversity of salt lakes in xinjiang, water and sediment samples were collected from two salt lakes, namely aibi salt lake and aiding salt lake, and the halophilic archaeal diversity of these samples was determined. totally eighty-six halophilic archeal strains, of whic ... | 2006 | 16736571 |
| phylogenetic relationships within the order halobacteriales inferred from 16s rrna gene sequences. | phylogenetic relationships within the halophilic archaea were inferred from comparisons of the 16s rrna gene sequences from 61 strains, representing 18 genera with validly published names within the order halobacteriales. trees produced using distance-matrix (least-squares and neighbour-joining) methods affirm with strong bootstrap support that the members of the order halobacteriales are a monophyletic group. ten genera were supported as monophyletic groups [haloarcula, halobiforma, halococcus, ... | 2006 | 16738095 |
| isolation and functional expression of the bop gene from halobiforma lacisalsi. | a novel bop gene was described from halobiforma lacisalsi strain aj5(t), an extremely halophilic archaeon isolated from ayakekum lake, china. following six rounds of pcr amplification based on the conserved fragment of the bop gene, the complete sequence of the bop gene, including the 5' and 3' flanking regions of the conserved fragment, was obtained by the ligation-mediated pcr amplification (lpa) approach. the data presented provide us with further insight into the distribution of bop-like gen ... | 2009 | 17689228 |
| phylogenetic relationships within the family halobacteriaceae inferred from rpob' gene and protein sequences. | in order to clarify the current phylogeny of the haloarchaea, particularly the closely related genera that have been difficult to sort out using 16s rrna gene sequences, the dna-dependent rna polymerase subunit b' gene (rpob') was used as a complementary molecular marker. partial sequences of the gene were determined from 16 strains of the family halobacteriaceae. comparisons of phylogenetic trees inferred from the gene and protein sequences as well as from corresponding 16s rrna gene sequences ... | 2007 | 17911299 |
| emended descriptions of genera of the family halobacteriaceae. | the family halobacteriaceae currently contains 96 species whose names have been validly published, classified in 27 genera (as of september 2008). in recent years, many novel species have been added to the established genera but, in many cases, one or more properties of the novel species do not agree with the published descriptions of the genera. authors have often failed to provide emended genus descriptions when necessary. following discussions of the international committee on systematics of ... | 2009 | 19244452 |
| archaeal diversity in the haloalkaline lake elmenteita in kenya. | a non-culture approach was used to study the archaeal diversity in lake elmenteita, kenya. five different sampling points were selected randomly within the lake. wet sediments and water samples were collected from each sampling point. in addition, dry mud cake was collected from three points where the lake had dried. dna was extracted from these samples and the 16s rrna genes were amplified using primers described to be domain-specific for archaea. eleven clone libraries were constructed using p ... | 2010 | 19806396 |
| further refinement of the phylogeny of the halobacteriaceae based on the full-length rna polymerase subunit b' (rpob') gene. | a considerable number of species of the halobacteriaceae possess multiple copies of the 16s rrna gene that exhibit more than 5 % divergence, complicating phylogenetic interpretations. two additional problems have been pointed out: (i) the genera haloterrigena and natrinema show a very close relationship, with some species being shown to overlap in phylogenetic trees reconstructed by the neighbour-joining method, and (ii) alkaliphilic and neutrophilic species of the genus natrialba form definitel ... | 2010 | 19946058 |
| natronoarchaeum mannanilyticum gen. nov., sp. nov., an aerobic, extremely halophilic archaeon isolated from commercial salt. | strain ysm-123(t) was isolated from commercial salt made from japanese seawater in niigata prefecture. optimal nacl and mg(2+) concentrations for growth were 4.0-4.5 m and 5 mm, respectively. the isolate was a mesophilic and slightly alkaliphilic haloarchaeon, whose optimal growth temperature and ph were 37 °c and ph 8.0-9.0. phylogenetic analysis based on 16s rrna gene sequence analysis suggested that strain ysm-123(t) is a member of the phylogenetic group defined by the family halobacteriaceae ... | 2010 | 19965994 |
| haloarchaeobius iranensis gen. nov., sp. nov., an extremely halophilic archaeon isolated from the saline lake aran-bidgol, iran. | strain eb21t was isolated from a brine sample of aran-bidgol salt lake, a saline playa in iran. strain eb21t was orange-red pigmented, motile rod and required at least 2 m nacl but not mgcl(2) for growth. optimal growth was achieved at 3.5 m nacl and 0.2 m mgcl(2). the optimum ph and temperature for growth were ph 7.5 and 40 -¦c, respectively, while it was able to grow over a ph range and a temperature range of 6.0-8.0 and 25-55 -¦c, respectively. analysis of 16s rrna gene sequence revealed that ... | 2011 | 21685256 |
| genome sequence of halobiforma lacisalsi aj5, an extremely halophilic archaeon which harbors a bop gene. | the draft genome sequence (4,398,155 bp, with 65.35% g+c content) of halobiforma lacisalsi aj5, an extremely halophilic archaeon isolated from a salt lake, is reported here. this is the first genome report for a species of the halobiforma genus. | 2011 | 22123770 |