| variation of 16s-23s rrna intergenic spacer regions (isrs) in acinetobacter baylyi (strain b2) isolated from activated sludge. | to determine the variability of the 16s-23s rrna intergenic spacer region (isr) of the newly described acinetobacter baylyi, 88 clones containing isr amplicons were screened and 14 chosen for further analysis. two different sized 16s-23s rrna isrs were distinguished comprising five variable and four conserved nucleotide blocks. the major regions of heterogeneity between the different sized isrs were due to blocks of substitutions with unique secondary structures interspersed with nucleotide subs ... | 2004 | 15368854 |
| naturally transformable acinetobacter sp. strain adp1 belongs to the newly described species acinetobacter baylyi. | genotypic and phenotypic analyses were carried out to clarify the taxonomic position of the naturally transformable acinetobacter sp. strain adp1. transfer tdna-pcr fingerprinting, 16s rrna gene sequence analysis, and selective restriction fragment amplification (amplified fragment length polymorphism analysis) indicate that strain adp1 and a second transformable strain, designated 93a2, are members of the newly described species acinetobacter baylyi. transformation assays demonstrate that the a ... | 2006 | 16391138 |
| estimation of ribosomal rna operon (rrn) copy number in acinetobacter isolates and potential of patterns of rrn operon-containing fragments for typing strains of members of this genus. | the copy number of the rrn operon in 70 strains of acinetobacter including the type strains of almost all the genomic species with validated names was estimated after digestion of their genomic dna by the restriction enzymes bglii and psti, and southern blotting. copy number estimates varied between and among species, with between 3 and 7 rrn operon copies detected. copy number estimates obtained from the same strain with the two enzymes sometimes varied. bglii generated rflp patterns of the rrn ... | 2006 | 16564958 |
| a constitutively expressed, truncated umudc operon regulates the reca-dependent dna damage induction of a gene in acinetobacter baylyi strain adp1. | in response to environmentally caused dna damage, sos genes are up-regulated due to reca-mediated relief of lexa repression. in escherichia coli, the sos umudc operon is required for dna damage checkpoint functions and for replicating damaged dna in the error-prone process called sos mutagenesis. in the model soil bacterium acinetobacter baylyi strain adp1, however, the content, regulation, and function of the umudc operon are unusual. the umuc gene is incomplete, and a remnant of an isehe3-like ... | 2006 | 16751513 |
| engineered cyanophycin synthetase (cpha) from nostoc ellipsosporum confers enhanced cpha activity and cyanophycin accumulation to escherichia coli. | the cyanophycin (cgp) synthetase gene (cphane1) of the transposon-induced argl mutant ne1 of the cyanobacterium nostoc ellipsosporum, which exhibits a cgp-leaky phenotype during diazotrophical growth, was cloned and expressed in escherichia coli strain top10. its amino acid sequence exhibited high similarities to cphas of other cyanobacteria. recombinant cells of e. coli, which harbored a fragment comprising the complete cphane1 gene plus 400 bp of its downstream region in colinear orientation t ... | 2006 | 17012590 |
| multiple-level regulation of genes for protocatechuate degradation in acinetobacter baylyi includes cross-regulation. | the bacterium acinetobacter baylyi uses the branched beta-ketoadipate pathway to metabolize aromatic compounds. here, the multiple-level regulation of expression of the pca-qui operon encoding the enzymes for protocatechuate and quinate degradation was studied. it is shown that both activities of the iclr-type regulator protein pcau at the structural gene promoter pcaip, namely protocatechuate-dependent activation of pca-qui operon expression as well as repression in the absence of protocatechua ... | 2007 | 17085716 |
| genes involved in intrinsic antibiotic resistance of acinetobacter baylyi. | bacterial genes defining intrinsic resistance to antibiotics encode proteins that can be targeted by antibiotic potentiators. to find such genes, a transposon insertion library of acinetobacter baylyi was screened with subinhibitory concentrations of various antibiotics to find supersusceptible mutants. a dna microarray printer was used to replica plate 10,000 individual library clones to select mutants unable to grow at 1/10 the mics of 12 different antibiotics. transposon insertions in 11 gene ... | 2006 | 16940057 |
| sequencing of the rpob gene and flanking spacers for molecular identification of acinetobacter species. | acinetobacter species are defined on the basis of several phenotypic characters, results of dna-dna homology, and more recently, similarities or dissimilarities in 16s rrna gene sequences. however, the 16s rrna gene is not polymorphic enough to clearly distinguish all acinetobacter species. we used an rna polymerase beta-subunit gene (rpob)-based identification scheme for the delineation of species within the genus acinetobacter, and towards that end, we determined the complete rpob gene and fla ... | 2006 | 16517861 |
| catm regulation of the benabcde operon: functional divergence of two lysr-type paralogs in acinetobacter baylyi adp1. | two lysr-type transcriptional regulators, benm and catm, control benzoate consumption by the soil bacterium acinetobacter baylyi adp1. these homologs play overlapping roles in the expression of multiple genes. this study focuses on the benabcde operon, which initiates benzoate catabolism. at this locus, benm and catm each activate transcription in response to the catabolite cis,cis-muconate. benm, but not catm, additionally responds to benzoate as an effector. regulation by catm alone is insuffi ... | 2006 | 16517618 |
| mage: a microbial genome annotation system supported by synteny results. | magnifying genomes (mage) is a microbial genome annotation system based on a relational database containing information on bacterial genomes, as well as a web interface to achieve genome annotation projects. our system allows one to initiate the annotation of a genome at the early stage of the finishing phase. mage's main features are (i) integration of annotation data from bacterial genomes enhanced by a gene coding re-annotation process using accurate gene models, (ii) integration of results o ... | 2006 | 16407324 |
| opportunities for genetic investigation afforded by acinetobacter baylyi, a nutritionally versatile bacterial species that is highly competent for natural transformation. | the genetic and physiological properties of acinetobacter baylyi strain adp1 make it an inviting subject for investigation of the properties underlying its nutritional versatility. the organism possesses a relatively small genome in which genes for most catabolic functions are clustered in several genetic islands that, unlike pathogenicity islands, give little evidence of horizontal transfer. coupling mutagenic polymerase chain reaction to natural transformation provides insight into how structu ... | 2005 | 16153178 |
| experimental methods for assaying natural transformation and inferring horizontal gene transfer. | the observation of frequent lateral acquisitions of genes in sequenced bacterial genomes has spurred experimental investigations to elucidate the factors governing ongoing gene transfer processes in bacteria. the uptake of naked dna by natural transformation is known to occur in a wide range of bacterial species and in some archaea. we describe a series of protocols designed to dissect the natural genetic transformability of individual bacterial strains under conditions that progress from standa ... | 2005 | 15865981 |
| seven novel species of acinetobacter isolated from activated sludge. | thirteen isolates of acinetobacter were obtained from activated sludge plants in victoria, australia. earlier 16s-23s rdna genomic fingerprinting and partial 16s rdna sequence data had suggested that these isolates might contain previously undescribed species. this view was confirmed here. a polyphasic taxonomic approach involving phenotypic characterization, near-complete 16s rdna sequence data and dna-dna hybridization analyses support the view that seven novel genomic species can be different ... | 2003 | 12892111 |
| lack of detectable dna uptake by bacterial gut isolates grown in vitro and by acinetobacter baylyi colonizing rodents in vivo. | biological risk assessment of food containing recombinant dna has exposed knowledge gaps related to the general fate of dna in the gastrointestinal tract (git). here, a series of experiments is presented that were designed to determine if genetic transformation of the naturally competent bacterium acinetobacter baylyi bd413 occurs in the git of mice and rats, with feed-introduced bacterial dna containing a kanamycin resistance gene (nptii). strain bd413 was found in various gut locations in germ ... | 2007 | 17961488 |
| an assessment of the potential of herbivorous insect gut bacteria to develop competence for natural transformation. | whereas the capability of dna uptake has been well established for numerous species and strains of bacteria grown in vitro, the broader distribution of natural transformability within bacterial communities remains largely unexplored. here, we investigate the ability of bacterial isolates from the gut of grass grub larvae (costelytra zealandica (white); coleoptera: scarabaeidae) to develop natural genetic competence in vitro. a total of 37 mostly species-divergent strains isolated from the gut of ... | 2007 | 17961487 |
| transformation of acinetobacter baylyi in non-sterile soil using recombinant plant nuclear dna. | to provide estimates of horizontal gene transfer from transgenic crops to indigenous soil bacteria, transformation frequencies were obtained for naturally transformable acinetobacter baylyi bd413 using a chromosomally integrated plant transgene. the transgene comprised sequences for two phenotypic markers: kanamycin resistance (npt ii) and green fluorescent protein (gfp), expressed from their own bacterial promoters. recipient bacteria carried a copy of these two genes, with deletions in their 3 ... | 2007 | 17961484 |
| screening of rhizosphere and soil bacteria for transformability. | natural transformation is assumed to be the most likely mechanism by which dna from transgenic plants could be horizontally transferred to bacteria. in order to determine the occurrence of naturally transformable bacteria amongst bulk and rhizosphere soil bacteria, different transformation strategies were employed using either plasmid dna (incq plasmids psm1890 and psm1885, conferring gfp, sm(r), gm(r) and gfp, sm(r), tc(r), respectively) or genomic dna from rhizosphere isolates, which were chro ... | 2007 | 17961483 |
| assessment of transformability of bacteria associated with tomato and potato plants. | transformation of plant-associated bacteria by plant dna has never been demonstrated in agricultural fields. in total 552 bacterial isolates from stems of ralstonia solanacearum-infected and healthy tomato plants and from stems and leaves of healthy potato plants were tested for natural genetic competence using plasmid psktg dna and homologous dna extracts. control strain acinetobacter baylyi adp1 was transformable with both dna extracts. no transformable isolates were observed after treatment w ... | 2007 | 17961482 |
| detection of potential transgenic plant dna recipients among soil bacteria. | the likelihood of gene transfer from transgenic plants to bacteria is dependent on gene number and the presence of homologous sequences. the large number of transgene copies in transplastomic (transgenes contained in the chloroplast genome) plant cells as well as the prokaryotic origin of the transgene, may thus significantly increase the likelihood of gene transfer to bacteria that colonize plant tissues. in order to assess the probability of such transfer, the length of homologous dna sequence ... | 2007 | 17961481 |
| influence of flanking homology and insert size on the transformation frequency of acinetobacter baylyi bd413. | reca-mediated recombination requires regions of homology between donor and recipient dna for successful integration. this paper investigates the effect of the relationship between the length of gene-sized inserts (434, 733, 2228 and 2400 bp) and flanking sequence homology (100 - ca. 11 000 bp) on transformation frequency in acinetobacter baylyi strain bd413. both insert size and size of the homologous region were varied, which improves on previous studies that kept insert size constant and varie ... | 2007 | 17961480 |
| genetic code ambiguity confers a selective advantage on acinetobacter baylyi. | a primitive genetic code, composed of a smaller set of amino acids, may have expanded via recursive periods of genetic code ambiguity that were followed by specificity. here we model a step in this process by showing how genetic code ambiguity could result in an enhanced growth rate in acinetobacter baylyi. | 2007 | 17616603 |
| cloning and characterization of a gene involved in triacylglycerol biosynthesis and identification of additional homologous genes in the oleaginous bacterium rhodococcus opacus pd630. | the oleaginous bacterium rhodococus opacus strain pd630 serves as a model organism to investigate the metabolism of storage triacylglycerols (tags) in bacteria. the key enzyme catalysing the last step of tag biosynthesis in bacteria is a promiscuous acyltransferase (atf), exhibiting acyl-coa acyltransferase activity to both diacylglycerols (dgat activity) and fatty alcohols (wax ester synthase, ws activity). an 800 bp pcr product was obtained from chromosomal dna of strain pd630 by using degener ... | 2008 | 18667565 |
| deletions of recbcd or recd influence genetic transformation differently and are lethal together with a recj deletion in acinetobacter baylyi. | in prokaryotes, homologous recombination is essential for the repair of genomic dna damage and for the integration of dna taken up during horizontal gene transfer. in escherichia coli, the exonucleases recj (specific for 5' single-stranded dna) and recbcd (degrades duplex dna) play important roles in recombination and recombinational double-strand break (dsb) repair by the recf and recbcd pathways, respectively. the cloned recj of acinetobacter baylyi partially complemented an e. coli recj mutan ... | 2007 | 17600070 |
| the recj dnase strongly suppresses genomic integration of short but not long foreign dna fragments by homology-facilitated illegitimate recombination during transformation of acinetobacter baylyi. | homology-facilitated illegitimate recombination (hfir) promotes genomic integration of foreign dna with a single segment homologous to the recipient genome by homologous recombination in the segment accompanied by illegitimate fusion of the heterologous sequence. during natural transformation of acinetobacter baylyi hfir occurs at about 0.01% of the frequency of fully homologous recombination. the role of the 5' single-strand-specific exonuclease recj in hfir was investigated. deletion of recj i ... | 2007 | 17462017 |
| molecular characterization of the gene encoding a new ampc beta-lactamase in acinetobacter baylyi. | the main objective of the present study was to demonstrate the presence of a beta-lactamase ampc gene in the chromosome of the non-pathogenic bacterium acinetobacter baylyi adp1. | 2007 | 17403709 |
| a double kill gene cassette for the positive selection of transforming non-selective dna segments in acinetobacter baylyi bd413. | natural transformation has been widely used for the monitoring of dna in biological and environmental samples. these assays depended on selectable traits on the tested dna. we have now developed a transformation assay system in which recombinational removal of a cassette with two conditional kill genes (hok and sacb) from the recipient genome provides positive selection for non-selective dna. the cassette was integrated into the acinetobacter baylyi bd413 chromosome within trpe and was flanked b ... | 2007 | 17250911 |
| discovery of a new prokaryotic type i gtp cyclohydrolase family. | gtp cyclohydrolase i (gcyh-i) is the first enzyme of the de novo tetrahydrofolate biosynthetic pathway present in bacteria, fungi, and plants, and encoded in escherichia coli by the fole gene. it is also the first enzyme of the biopterin (bh4) pathway in homo sapiens, where it is encoded by a homologous fole gene. a homology-based search of gcyh-i orthologs in all sequenced bacteria revealed a group of microbes, including several clinically important pathogens, that encoded all of the enzymes of ... | 2006 | 17032654 |
| rapid evolution of diminished transformability in acinetobacter baylyi. | the reason for genetic exchange remains a crucial question in evolutionary biology. acinetobacter baylyi strain adp1 is a highly competent and recombinogenic bacterium. we compared the parallel evolution of wild-type and engineered noncompetent lineages of a. baylyi in the laboratory. if transformability were to result in an evolutionary benefit, it was expected that competent lineages would adapt more rapidly than noncompetent lineages. instead, regardless of competency, lineages adapted to the ... | 2006 | 17028281 |
| microdiesel: escherichia coli engineered for fuel production. | biodiesel is an alternative energy source and a substitute for petroleum-based diesel fuel. it is produced from renewable biomass by transesterification of triacylglycerols from plant oils, yielding monoalkyl esters of long-chain fatty acids with short-chain alcohols such as fatty acid methyl esters and fatty acid ethyl esters (faees). despite numerous environmental benefits, a broader use of biodiesel is hampered by the extensive acreage required for sufficient production of oilseed crops. ther ... | 2006 | 16946248 |
| sensitivity of an acinetobacter baylyi mpl mutant to dna damage. | a mutant derived from acinetobacter baylyi adp1 was isolated from a screen for genes involved in the response to dna damage. this derivative has an insertion in the mpl gene which encodes a peptidoglycan-recycling protein. we demonstrate that the insertion renders cells sensitive to mitomycin c and to uv. | 2008 | 18083886 |
| deletion mutations caused by dna strand slippage in acinetobacter baylyi. | short nucleotide sequence repetitions in dna can provide selective benefits and also can be a source of genetic instability arising from deletions guided by pairing between misaligned strands. these findings raise the question of how the frequency of deletion mutations is influenced by the length of sequence repetitions and by the distance between them. an experimental approach to this question was presented by the heat-sensitive phenotype conferred by pcag1102, a 30-bp deletion in one of the st ... | 2006 | 16885271 |
| visual evidence of horizontal gene transfer between plants and bacteria in the phytosphere of transplastomic tobacco. | plant surfaces, colonized by numerous and diverse bacterial species, are often considered hot spots for horizontal gene transfer (hgt) between plants and bacteria. plant dna released during the degradation of plant tissues can persist and remain biologically active for significant periods of time, suggesting that soil or plant-associated bacteria could be in direct contact with plant dna. in addition, nutrients released during the decaying process may provide a copiotrophic environment conducive ... | 2009 | 19329660 |
| strategy for in situ detection of natural transformation-based horizontal gene transfer events. | a strategy is described that enables the in situ detection of natural transformation in acinetobacter baylyi bd413 by the expression of a green fluorescent protein. microscale detection of bacterial transformants growing on plant tissues was shown by fluorescence microscopy and indicated that cultivation-based selection of transformants on antibiotic-containing agar plates underestimates transformation frequencies. | 2008 | 18165369 |
| double illegitimate recombination events integrate dna segments through two different mechanisms during natural transformation of acinetobacter baylyi. | acquisition of foreign dna by horizontal gene transfer is seen as a major source of genetic diversity in prokaryotes. however, strongly divergent dna is not genomically integrated by homologous recombination and would depend on illegitimate recombination (ir) events which are rare. we show that, by two mechanisms, during natural transformation of acinetobacter baylyi two ir events can integrate dna segments. one mechanism is double illegitimate recombination (dir) acting in the absence of any ho ... | 2008 | 18194157 |
| the thin pili of acinetobacter sp. strain bd413 mediate adhesion to biotic and abiotic surfaces. | two structurally different appendages, thin and thick pili, are found in members of the genus acinetobacter. the presence of pilus structures correlates with different phenotypes, such as adherence to surfaces, a trait not only observed in pathogenic acinetobacter species, as well as motility. however, their distinct individual roles were unknown. to characterize the role of different pili in the physiology of acinetobacter, we isolated the thin pili from the cell surface of acinetobacter sp. st ... | 2006 | 16461692 |
| neutral lipid biosynthesis in engineered escherichia coli: jojoba oil-like wax esters and fatty acid butyl esters. | wax esters are esters of long-chain fatty acids and long-chain fatty alcohols which are of considerable commercial importance and are produced on a scale of 3 million tons per year. the oil from the jojoba plant (simmondsia chinensis) is the main biological source of wax esters. although it has a multitude of potential applications, the use of jojoba oil is restricted, due to its high price. in this study, we describe the establishment of heterologous wax ester biosynthesis in a recombinant esch ... | 2006 | 16461689 |
| relevance of nucleotides of the pcau binding site from acinetobacter baylyi. | results from a random mutagenesis procedure on the pcau binding site from acinetobacter baylyi followed by in vivo and in vitro screening are presented. pcau is an iclr-type transcriptional regulator from the soil bacterium a. baylyi and is required for the regulated expression of enzymes for protocatechuate and quinate degradation encoded by the pca-qui operon. it binds to a 45 bp area located in the pcau-pcai intergenic region which consists of three perfect 10 bp sequence repeats forming one ... | 2008 | 18310022 |
| multiple pathways for triacylglycerol biosynthesis in streptomyces coelicolor. | the terminal reaction in triacylglyceride (tag) biosynthesis is the esterification of diacylglycerol (dag) with a fatty acid molecule. to study this reaction in streptomyces coelicolor, we analyzed three candidate genes (sco0958, sco1280, and sco0123) whose products significantly resemble the recently identified wax ester synthase/acyl-coenzyme a (coa):dag acyltransferase (dgat) from acinetobacter baylyi. the deletion of either sco0123 or sco1280 resulted in no detectable decrease in tag accumul ... | 2008 | 18310412 |
| characterizing the regulation of the pu promoter in acinetobacter baylyi adp1. | effective gene trapping and screening requires sensory and regulatory compatibility of both host and exogenous systems. the naturally competent bacterium acinetobacter baylyi adp1 is able to efficiently take up and integrate exogenous dna into the chromosome, making it an attractive host system for a wide range of metagenomic applications. to test the ability of a. baylyi adp1 to express the xylr-regulated pu promoter from pseudomonas putida mt-2, we have constructed and examined an a. baylyi ad ... | 2008 | 18363715 |
| new insights into the alternative d-glucarate degradation pathway. | although the d-glucarate degradation pathway is well characterized in escherichia coli, genetic and biochemical information concerning the alternative pathway proposed in pseudomonas species and bacillus subtilis remains incomplete. acinetobacter baylyi adp1 is a gram-negative soil bacterium possessing the alternative pathway and able to grow using d-glucarate as the only carbon source. based on the annotation of its sequenced genome (1), we have constructed a complete collection of singlegene d ... | 2008 | 18364348 |
| effects of rhizodeposition of non-transgenic and transplastomic tobaccos on the soil bacterial community. | the effect of root-released compounds of transplastomic tobacco (nicotiana tabacum) on the soil bacterial community structure, and their potential to support horizontal gene transfer (hgt) to bacteria have been studied. soil microcosms were exposed to root-released compounds collected from transplastomic and non-transgenic tobacco cultivars. cluster analysis of automated ribosomal intergenic spacer analysis (arisa) profiles of the soil bacterial community after 48 h incubation grouped the transg ... | 2008 | 18384726 |
| bacterial acyltransferases as an alternative for lipase-catalyzed acylation for the production of oleochemicals and fuels. | bacterial acyltransferases are a new class of enzymes, and the first member was identified as ws/dgat in acinetobacter baylyi adp1. their unspecificity have been used in several biotechnological applications for lipid modification, a field that has been dominated by the use of lipases. examples are the biosynthesis of jojoba-like wax esters and fatty-acid ethyl esters. in addition, these enzymes are also capable of synthesizing acylthioesters. acyloxoesters and acylthioesters can thus be produce ... | 2008 | 18399520 |
| whole-genome pyrosequencing of an epidemic multidrug-resistant acinetobacter baumannii strain belonging to the european clone ii group. | the whole-genome sequence of an epidemic, multidrug-resistant acinetobacter baumannii strain (strain acicu) belonging to the european clone ii group and carrying the plasmid-mediated bla(oxa)(-)(58) carbapenem resistance gene was determined. the a. baumannii acicu genome was compared with the genomes of a. baumannii atcc 17978 and acinetobacter baylyi adp1, with the aim of identifying novel genes related to virulence and drug resistance. a. baumannii acicu has a single chromosome of 3,904,116 bp ... | 2008 | 18411315 |
| unique organization of the 16s-23s intergenic spacer regions of strains of acinetobacter baylyi provides a means for its identification from other acinetobacter species. | this paper extends an earlier report on rrn operon characteristics in members of the genus acinetobacter. it describes a systematic approach towards developing and validating a protocol for elucidating how the intergenic spacer regions (isr) in acinetobacter baylyi strains are organized and allows the numbers of long and short isrs to be determined. experimental data confirmed the in silico predictions based on available a. baylyi rrn sequence data. all were shown to possess three long isrs and ... | 2008 | 18436316 |
| acinetobacter baylyi as a pathogen for opportunistic infection. | there are no previous reports of human infection due to acinetobacter baylyi. in this study, we report on six patients with bacteremia due to a. baylyi, based on analysis of the 16s-23s rrna intergenic spacer and the 16s rrna gene. all six patients had multiple underlying diseases. the infection was nosocomially acquired in five patients. the six clinical isolates had similar ribopatterns, suggesting a clonal relationship. compared to the reference strain, the clinical isolates were more resista ... | 2008 | 18632910 |
| the recbcd and sbccd dnases suppress homology-facilitated illegitimate recombination during natural transformation of acinetobacter baylyi. | during natural transformation of acinetobacter baylyi, the genomic integration of foreign (non-homologous) dna is possible when the dna contains a single segment homologous to the recipient genome (anchor) through homologous recombination in the anchor facilitating illegitimate recombination in the neighbouring foreign dna (homology-facilitated illegitimate recombination; hfir). dna integration by hfir occurs about 10 000 times less frequently than fully homologous recombination, but at least 10 ... | 2008 | 18667576 |
| aromatic degradative pathways in acinetobacter baylyi underlie carbon catabolite repression. | carbon catabolite repression is an important mechanism allowing efficient carbon source utilization. in the soil bacterium acinetobacter baylyi, this mechanism has been shown to apply to the aromatic degradative pathways for the substrates protocatechuate, p-hydroxybenzoate and vanillate. in this investigation, transcriptional fusions with the gene for luciferase in the gene clusters for the degradation of benzyl esters, anthranilate, benzoate, hydroxycinnamates and dicarboxylates (are, ant, ben ... | 2008 | 18832315 |
| iterative reconstruction of a global metabolic model of acinetobacter baylyi adp1 using high-throughput growth phenotype and gene essentiality data. | genome-scale metabolic models are powerful tools to study global properties of metabolic networks. they provide a way to integrate various types of biological information in a single framework, providing a structured representation of available knowledge on the metabolism of the respective species. | 2008 | 18840283 |
| comparative genome sequence analysis of multidrug-resistant acinetobacter baumannii. | the recent emergence of multidrug resistance (mdr) in acinetobacter baumannii has raised concern in health care settings worldwide. in order to understand the repertoire of resistance determinants and their organization and origins, we compared the genome sequences of three mdr and three drug-susceptible a. baumannii isolates. the entire mdr phenotype can be explained by the acquisition of discrete resistance determinants distributed throughout the genome. a comparison of closely related mdr and ... | 2008 | 18931120 |
| frequent integration of short homologous dna tracks during acinetobacter baylyi transformation and influence of transcription and recj and sbccd dnases. | the minimal length of integrated homologous donor dna tracks in acinetobacter baylyi transformation and factors influencing the location and length of tracks were determined. donor dna contained the nptii gene region (kanamycin resistance, kmr). this region carried nine approximately evenly spaced silent nucleotide sequence tags and was embedded in heterologous dna. recipient cells carried the normal nptii gene with a central 10 bp deletion (kanamycin-sensitive). the km(r) transformants obtained ... | 2008 | 19047735 |
| wide dissemination of oxa-type carbapenemases in clinical acinetobacter spp. isolates from south korea. | carbapenem-resistant acinetobacter spp. are being increasingly reported worldwide, including in south korea, where we examined 144 representative isolates collected in a nationwide hospital survey in 2005. metallo-beta-lactamases were detected in only 19.4% of isolates, none of which were acinetobacter baumannii, whereas 74.3% of isolates (mostly a. baumannii) expressed bla(oxa) carbapenemase genes. among the latter, 47 had bla(oxa-23)-like genes and 56 had upregulated bla(oxa-51)-like variants, ... | 2009 | 19091520 |
| structure and substrate specificity of acetyltransferase aciad1637 from acinetobacter baylyi adp1. | gene aciad1637 from acinetobacter baylyi adp1 encodes a 182 amino acid putative antibiotic resistance protein. the structure of this protein (termed acepita) has been solved in space group p(2) to 2.35 a resolution. acepita belongs to the gcn5-related n-acetyltransferase (gnat) family, and contains the four sequence motifs conserved among family members. the structure of acepita is compared with that of pita, its homologue from pseudomonas aeruginosa. acepita has a similar substrate profile to p ... | 2009 | 19135125 |
| role of acinetobacter baylyi crc in catabolite repression of enzymes for aromatic compound catabolism. | here, we describe for the first time the crc (catabolite repression control) protein from the soil bacterium acinetobacter baylyi. expression of a. baylyi crc varied according to the growth conditions. a strain with a disrupted crc gene showed the same growth as the wild type on a number of carbon sources. carbon catabolite repression by acetate and succinate of protocatechuate 3,4-dioxygenase, the key enzyme of protocatechuate breakdown, was strongly reduced in the crc strain, whereas in the wi ... | 2009 | 19201803 |
| cloning, expression, and characterization of a novel diketoreductase from acinetobacter baylyi. | reductions of carbonyl groups catalyzed by oxidoreductases are involved in all biological processes and are often a class of important biocatalyst. in this article, we report a novel enzyme designated as diketoreductase (dkr) that was able to reduce two carbonyl groups in a diketo ester to corresponding dihydroxy ester with excellent stereoselectivity. the dkr was cloned from acinetobacter baylyi by reverse genetic method, heterogeneously expressed in escherichia coli, and purified to homogeneit ... | 2009 | 19204834 |
| galleria mellonella as a model system to study acinetobacter baumannii pathogenesis and therapeutics. | nonmammalian model systems of infection such as galleria mellonella (caterpillars of the greater wax moth) have significant logistical and ethical advantages over mammalian models. in this study, we utilize g. mellonella caterpillars to study host-pathogen interactions with the gram-negative organism acinetobacter baumannii and determine the utility of this infection model to study antibacterial efficacy. after infecting g. mellonella caterpillars with a reference a. baumannii strain, we observe ... | 2009 | 19332683 |
| inducer responses of benm, a lysr-type transcriptional regulator from acinetobacter baylyi adp1. | benm and catm control transcription of a complex regulon for aromatic compound degradation. these acinetobacter baylyi paralogues belong to the largest family of prokaryotic transcriptional regulators, the lysr-type proteins. whereas benm activates transcription synergistically in response to two effectors, benzoate and cis,cis-muconate, catm responds only to cis,cis-muconate. here, site-directed mutagenesis was used to determine the physiological significance of an unexpected benzoate-binding p ... | 2009 | 19400783 |
| analysis of neutral lipid biosynthesis in streptomyces avermitilis ma-4680 and characterization of an acyltransferase involved herein. | the physiology of lipid production in streptomyces avermitilis ma-4680 with regard to the fatty acid composition of the accumulated lipids and their cellular distribution was analyzed. cells were able to accumulate about ten to 30 lipid granules with diameters between 100 and 500 nm filling about 70-80% of the cell cytoplasm. gas chromatography/mass spectrometry analyses of total cellular lipids and from isolated triacylglycerols (tag) confirmed a similar fatty acid composition with a large port ... | 2009 | 19424691 |
| sexual isolation in acinetobacter baylyi is locus-specific and varies 10,000-fold over the genome. | naturally transformable bacteria acquire chromosomal dna from related species at lower frequencies than from cognate dna sources. to determine how genome location affects heterogamic transformation in bacteria, we inserted an npti marker into random chromosome locations in 19 different strains of the acinetobacter genus (>24% divergent at the muts/trpe loci). dna from a total of 95 npti-tagged isolates was used to transform the recipient acinetobacter baylyi strain adp1. a total of >1300 transfo ... | 2009 | 19474200 |
| the acinetobacter baylyi hfq gene encodes a large protein with an unusual c terminus. | in gammaproteobacteria the hfq protein shows a great variation in size, especially in its c-terminal part. extremely large hfq proteins consisting of almost 200 amino acid residues and more are found within the gammaproteobacterial family moraxellaceae. the difference in size compared to other hfq proteins is due to a glycine-rich domain near the c-terminal end of the protein. acinetobacter baylyi, a nonpathogenic soil bacterium and member of the moraxellaceae encodes a large 174-amino-acid hfq ... | 2009 | 19561130 |
| identification of an inhibitor of the murc enzyme, which catalyzes an essential step in the peptidoglycan precursor synthesis pathway. | the pathway for synthesis of the peptidoglycan precursor udp-n-acetylmuramyl pentapeptide is essential in gram-positive and gram-negative bacteria. this pathway has been exploited in the recent past to identify potential new antibiotics as inhibitors of one or more of the mur enzymes. in the present study, a high-throughput screen was employed to identify potential inhibitors of the escherichia coli murc (udp-n-acetylmuramic acid:l-alanine ligase), the first of four paralogous amino acid-adding ... | 2008 | 18315498 |
| a complete collection of single-gene deletion mutants of acinetobacter baylyi adp1. | we have constructed a collection of single-gene deletion mutants for all dispensable genes of the soil bacterium acinetobacter baylyi adp1. a total of 2594 deletion mutants were obtained, whereas 499 (16%) were not, and are therefore candidate essential genes for life on minimal medium. this essentiality data set is 88% consistent with the escherichia coli data set inferred from the keio mutant collection profiled for growth on minimal medium, while 80% of the orthologous genes described as esse ... | 2008 | 18319726 |
| optimization of bacterial whole cell bioreporters for toxicity assay of environmental samples. | in a study to optimize bacterial whole cell biosensors (bioreporters) for the detection of environmental contaminants, we constructed a toxicity sensing strain acinetobacter baylyi adp1_reca_lux. the adp1_reca_lux is a chromosomally based bioreporter which makes the sensing system stable and negates the need for antibiotics to maintain the trait. the aop1_reca_lux is activated to express bioluminescence when it is exposed to dna damaging toxicants. since the adp1_reca_lux constantly expresses a ... | 2009 | 19921916 |
| the beta-ketoadipate pathway of acinetobacter baylyi undergoes carbon catabolite repression, cross-regulation and vertical regulation, and is affected by crc. | the degradation of many structurally diverse aromatic compounds in acinetobacter baylyi is accomplished by the beta-ketoadipate pathway. in addition to specific induction of expression by certain aromatic compounds, this pathway is regulated by complex mechanisms at multiple levels, which are the topic of this study. multiple operons feeding into the beta-ketoadipate pathway are controlled by carbon catabolite repression (ccr) caused by succinate plus acetate. the pathways under study enable the ... | 2010 | 20110298 |
| isolation and characterization of a novel thermophilic-organic solvent stable lipase from acinetobacter baylyi. | the benzene tolerant acinetobacter baylyi isolated from marine sludge in angsila, thailand could constitutively secrete lipolytic enzymes. the enzyme was successfully purified 21.89-fold to homogeneity by ammonium sulfate precipitation and gel-permeable column chromatography with a relative molecular mass as 30 kda. the enzyme expressed maximum activity at 60 degrees c and ph 8.0 with p-nitrophenyl palmitate as a substrate and found to be stable in ph and temperature ranging from 6.0-9.0 to 60-8 ... | 2010 | 20177822 |
| hybrid antimicrobial enzyme and silver nanoparticle coatings for medical instruments. | we report a method for the synthesis of antimicrobial coatings on medical instruments that combines the bacteriolytic activity of lysozyme and the biocidal properties of silver nanoparticles. colloidal suspensions of lysozyme and silver nanoparticles were electrophoretically deposited onto the surface of stainless steel surgical blades and needles. electrodeposited films firmly adhered to stainless steel surfaces even after extensive washing and retained the hydrolytic properties of lysozyme. th ... | 2009 | 20355960 |
| acinetobacter baylyi starvation-induced genes identified through incubation in long-term stationary phase. | acinetobacter species encounter cycles of feast and famine in nature. we show that populations of acinetobacter baylyi strain adp1 remain dynamic for 6 weeks in batch culture. we created a library of lacz reporters inserted into sali sites in the genome and then isolated 30 genes with lacz insertions whose expression was induced by starvation during long-term stationary phase compared with their expression during exponential growth. the genes encode metabolic, gene expression, dna maintenance, e ... | 2010 | 20511417 |
| the small rna aar in acinetobacter baylyi: a putative regulator of amino acid metabolism. | small non-coding rnas (srnas) are key players in prokaryotic metabolic circuits, allowing the cell to adapt to changing environmental conditions. regulatory interference by srnas in cellular metabolism is often facilitated by the sm-like protein hfq. a search for novel srnas in a. baylyi intergenic regions was performed by a biocomputational screening. one candidate, aar, encoded between trps and sucd showed hfq dependency in northern blot analysis. aar was expressed strongly during stationary g ... | 2010 | 20559624 |
| genomics-driven reconstruction of acinetobacter nad metabolism: insights for antibacterial target selection. | enzymes involved in the last steps of nad biogenesis, nicotinate mononucleotide adenylyltransferase (nadd) and nad synthetase (nade), are conserved and essential in most bacterial species and are established targets for antibacterial drug development. our genomics-based reconstruction of nad metabolism in the emerging pathogen acinetobacter baumannii revealed unique features suggesting an alternative targeting strategy. indeed, genomes of all analyzed acinetobacter species do not encode nadd, wh ... | 2010 | 20926389 |
| full-length structures of benm and two variants reveal different oligomerization schemes for lysr-type transcriptional regulators. | benm, a lysr-type transcriptional regulator (lttr) from the bacterium acinetobacter baylyi, responds synergistically to benzoate and cis,cis-muconate. with these effectors, benm activates gene expression during benzoate consumption. without effectors, benm represses transcription. here, x-ray crystallography was used to determine the full-length structures of benm and two variants that activate transcription without benzoate or cis,cis-muconate: benm(r156h) and benm(e226k). previous studies indi ... | 2010 | 20932977 |
| rational design of a plasmid origin that replicates efficiently in both gram-positive and gram-negative bacteria. | most plasmids replicate only within a particular genus or family. | 2010 | 20949038 |
| exposure of acinetobacter baylyi adp1 to the biocide chlorhexidine leads to acquired resistance to the biocide itself and to oxidants. | the aim of this study was to determine the effect of exposure to sublethal concentrations of chlorhexidine on oxidative stress protection by acinetobacter baylyi adp1. | 2010 | 21106564 |
| preparation of ethyl 3r,5s-6-(benzyloxy)-3,5-dihydroxy-hexanoate by recombinant diketoreductase in a biphasic system. | diketoreductase from acinetobacter baylyi atcc 33305 is a unique carbonyl reductase, which can stereoselectively reduce ethyl-6-(benzyloxy)-3,5-dioxohexanoate to ethyl 3r,5s-6-(benzyloxy)-3,5-dihydroxy-hexanoate, an advanced intermediate for statin drugs. in the present study, we explored an aqueous-organic biphasic reaction system to make this biocatalyst more practical and valuable. different from most oxidoreductases, diketoreductase displayed an excellent tolerance to certain organic solvent ... | 2010 | 21163644 |
| biodegradation pathways of chloroanilines by acinetobacter baylyi strain gfj2. | the acinetobacter baylyi strain gfj2 was isolated from soil that was potentially contaminated with herbicides. it exhibited complete biodegradations of 4-chlroaniline (4ca) and 3,4-dichloroaniline (34dca), a wide range of monohalogenated anilines (chloro-, bromo-, and fluoro-anilines) and other dichloroanilines. an in-depth investigation of the biodegradation pathway revealed that a dechlorination reaction may be involved in 34dca biodegradation, which forms 4ca as the first intermediate. by det ... | 2010 | 21177022 |
| metabolic production of a novel polymer feedstock, 3-carboxy muconate, from vanillin. | vanillin can be produced on a commercial scale by depolymerising renewable lignin. one product of microbial metabolism of vanillin by common soil microbes, such as acinetobacter baylyi, is a tricarboxylic acid with a butadiene backbone known as 3-carboxy muconate (3cm). three enzymes, 4-hydroxy benzaldehyde dehydrogenase, vanillate monooxygenase and protocatechuate 3,4-dioxygenase, catalyse the biotransformation of vanillin to 3cm. these three enzymes were metabolically engineered into an escher ... | 2011 | 21221570 |
| functionalization of whole-cell bacterial reporters with magnetic nanoparticle. | we developed a biocompatible and highly efficient approach for functionalization of bacterial cell wall with magnetic nanoparticles (mnps). three acinetobacter baylyi adp1 chromosomally based bioreporters, which were genetically engineered to express bioluminescence in response to salicylate, toluene/ xylene and alkanes, were functionalized with 18 3 nm iron oxide mnps to acquire magnetic function. the efficiency of mnps functionalization of acinetobacter bioreporters was 99.96 0.01%. the mnps-f ... | 2011 | 21255376 |
| high reliability transformation of the basal fungus mucor circinelloides by electroporation. | molecular approaches to study the biology of the zygomycete mucor circinelloides depend mainly on the existence of a polyethylene glycol-based transformation method, which is one of the most efficient in zygomycete fungi. however, the poor reliability and low transformation rates of this method are major obstacles in the molecular study of a number of biological processes. this paper describes an easy and reliable method to transform m. circinelloides protoplasts by electroporation. a high-volta ... | 2011 | 21256886 |
| improving qpcr efficiency in environmental samples by selective removal of humic acids with dax-8. | quantitative pcr is becoming the method of choice for the detection of pathogenic microorganisms and other targets in the environment. a major obstacle when amplifying dna is the presence of inhibiting substances like humic acids that decrease the efficiency of pcr. we combined the polymeric adsorbent supelite™ dax-8 with a large-volume (10 ml) nucleic acid extraction method to decrease the humic acid content prior to qpcr quantification in water samples. the method was tested by spiking with hu ... | 2011 | 21256890 |
| long-term diversity and genome adaptation of acinetobacter baylyi in a minimal-medium chemostat. | laboratory-based evolution experiments on microorganisms that do not recombine frequently show two distinct phases: an initial rapid increase in fitness followed by a slower regime. to explore the population structure and the evolutionary tree in the later stages of adaptation, we evolved a very large population (~3 × 10(10)) of acinetobacter baylyi bacteria for approximately 2,800 generations from a single clone. the population was maintained in a chemostat at a high dilution rate. nitrate in l ... | 2012 | 23254395 |
| salt adaptation in acinetobacter baylyi: identification and characterization of a secondary glycine betaine transporter. | members of the genus acinetobacter are well known for their metabolic versatility that allows them to adapt to different ecological niches. here, we have addressed how the model strain acinetobacter baylyi copes with different salinities and low water activities. a. baylyi tolerates up to 900 mm sodium salts and even higher concentrations of potassium chloride. growth at high salinities was better in complex than in mineral medium and addition of glycine betaine stimulated growth at high salinit ... | 2011 | 21567174 |
| improved triacylglycerol production in acinetobacter baylyi adp1 by metabolic engineering. | abstract: background: triacylglycerols are used in various purposes including food applications, cosmetics, oleochemicals and biofuels. currently the main sources for triacylglycerol are vegetable oils, and microbial triacylglycerol has been suggested as an alternative for these. due to the low production rates and yields of microbial processes, the role of metabolic engineering has become more significant. as a robust model organism for genetic and metabolic studies, and for the natural capabil ... | 2011 | 21592360 |
| de novo biosynthesis of biodiesel by escherichia coli in optimized fed-batch cultivation. | biodiesel is a renewable alternative to petroleum diesel fuel that can contribute to carbon dioxide emission reduction and energy supply. biodiesel is composed of fatty acid alkyl esters, including fatty acid methyl esters (fames) and fatty acid ethyl esters (faees), and is currently produced through the transesterification reaction of methanol (or ethanol) and triacylglycerols (tags). tags are mainly obtained from oilseed plants and microalgae. a sustainable supply of tags is a major bottleneck ... | 2011 | 21629774 |
| development of a new tool to improve gene transfer frequency calculations. | gene transfer frequency can be determined experimentally on plates, but the methods currently in use do not discriminate between independent transfers and clonal multiplication of initial transformants. in order to overcome this bias, we engineered an acinetobacter baylyi population in which cells differed by a specific molecular signature and used it as recipient in transformation experiments. our results suggest that a corrective factor of 0.52 should be applied in order to accurately report n ... | 2011 | 21640142 |
| natural transformation facilitates transfer of transposons, integrons and gene cassettes between bacterial species. | we have investigated to what extent natural transformation acting on free dna substrates can facilitate transfer of mobile elements including transposons, integrons and/or gene cassettes between bacterial species. naturally transformable cells of acinetobacter baylyi were exposed to dna from integron-carrying strains of the genera acinetobacter, citrobacter, enterobacter, escherichia, pseudomonas, and salmonella to determine the nature and frequency of transfer. exposure to the various dna sourc ... | 2012 | 22876180 |
| discovery of a gene involved in a third bacterial protoporphyrinogen oxidase activity through comparative genomic analysis and functional complementation. | tetrapyrroles are ubiquitous molecules in nearly all living organisms. heme, an iron containing tetrapyrrole, is widely distributed in nature including most characterized aerobic and facultative bacteria. a large majority of bacteria that contain heme possess the ability to synthesize it. despite this capability, and the fact that the biosynthetic pathway has been well studied, enzymes catalyzing at least three steps have remained "missing" in many bacteria. in the current work we have employed ... | 2011 | 21642412 |
| quantitative measurement of ph influence on salr regulated gene expression in acinetobacter baylyi adp1. | the influence of medium ph conditions on regulation of the sal operon was quantitatively measured by using a genetically engineered acinetobacter baylyi adp1 strain adpwh_lux. live-cell based bioluminescence assays determined that the salr, a lysr-type transcriptional regulator (lttr), functioned in a range of medium ph conditions. during exponential growth, salr regulated bioluminescence production peaked at the neutral ph and tolerated medium ph changes of 5.5-9.0. in the stationary phase, the ... | 2009 | 19616585 |
| acinetobacter baylyi adp1 as a model for metabolic system biology. | information produced by the annotation of an 'average bacterial genome' can be separated into three parts. one-third represents what we know, another third what we think we know, and the last third what we know we do not know. knowledge of metabolism is also described by this three thirds rule. understanding how a cell operates will require a better knowledge of the two ignored thirds of its parts. moreover, metabolism needs to be further investigated using organisms whose life styles are differ ... | 2009 | 19709925 |
| clinical carbapenem-resistant acinetobacter baylyi strain coharboring blasim-1 and blaoxa-23 from china. | bla(sim-1) and bla(oxa-23) were codetected in clinical carbapenem-resistant acinetobacter baylyi strain nb09a30. both of carbapenemase genes were located on a large plasmid (ca. 360 kb). bla(sim-1) was found as a gene cassette inserted into a class 1 integron identical to that determined in acinetobacter sp. isolates from south korea. the genetic structure of bla(oxa-23) in nb09a30 was different from that in the prevalent acinetobacter baumannii of clonal complex 92 (cc92) from the same hospital ... | 2011 | 21876057 |
| acinetobacter baylyi adp1: transforming the choice of model organism. | for more than 25 years, acinetobacter baylyi adp1 has been used in molecular biology studies that address a broad range of questions. recently, the rapid accumulation of data from dna sequencing, gene expression, protein structure, and other high-throughput methodology has increased the ability to tackle complex topics using sophisticated approaches to metabolic and genetic engineering. while the genetic malleability of adp1 makes it an ideal organism for such investigations, a. baylyi adp1 has ... | 2011 | 22034222 |
| transcriptomic and biochemical analyses identify a family of chlorhexidine efflux proteins. | chlorhexidine is widely used as an antiseptic or disinfectant in both hospital and community settings. a number of bacterial species display resistance to this membrane-active biocide. we examined the transcriptomic response of a representative nosocomial human pathogen, acinetobacter baumannii, to chlorhexidine to identify the primary chlorhexidine resistance elements. the most highly up-regulated genes encoded components of a major multidrug efflux system, adeab. the next most highly overexpre ... | 2013 | 24277845 |
| single-step selection of drug resistant acinetobacter baylyi adp1 mutants reveals a functional redundancy in the recruitment of multidrug efflux systems. | members of the genus acinetobacter have been the focus recent attention due to both their clinical significance and application to molecular biology. the soil commensal bacterium acinetobacter baylyi adp1 has been proposed as a model system for molecular and genetic studies, whereas in a clinical environment, acinetobacter spp. are of increasing importance due to their propensity to cause serious and intractable systemic infections. clinically, a major factor in the success of acinetobacter spp. ... | 2013 | 23409126 |
| whole-cell bacterial bioreporter for actively searching and sensing of alkanes and oil spills. | acinetobacter baylyi adp1 was found to tolerate seawater and have a special ability of adhering to an oil-water interface of 10-80 µm emulsified mineral and crude oil droplets. these properties make adp1 an ideal bacterial chassis for constructing bioreporters that are able to actively search and sense oil spill in water and soils. acinetobacter baylyi bioreporter adpwh_alk was developed and applied to the detection of alkanes and alkenes in water, seawater and soils. bioreporter adpwh_alk was a ... | 2012 | 21951420 |
| Real-time monitoring of intracellular wax ester metabolism. | Wax esters are industrially relevant molecules exploited in several applications of oleochemistry and food industry. At the moment, the production processes mostly rely on chemical synthesis from rather expensive starting materials, and therefore solutions are sought from biotechnology. Bacterial wax esters are attractive alternatives, and especially the wax ester metabolism of Acinetobacter sp. has been extensively studied. However, the lack of suitable tools for rapid and simple monitoring of ... | 2011 | 21961954 |
| identification of important residues in diketoreductase from acinetobacter baylyi by molecular modeling and site-directed mutagenesis. | diketoreductase (dkr) from acinetobacter baylyi exhibits a unique property of double reduction of a β, δ-diketo ester with excellent stereoselectivity, which can serve as an efficient biocatalyst for the preparation of an important chiral intermediate for cholesterol lowering statin drugs. taken the advantage of high homology between dkr and human heart 3-hydroxyacyl-coa dehydrogenase (had), a molecular model was created to compare the tertiary structures of dkr and had. in addition to the possi ... | 2011 | 21893158 |
| bacterial recombination promotes the evolution of multi-drug-resistance in functionally diverse populations. | bacterial recombination is believed to be a major factor explaining the prevalence of multi-drug-resistance (mdr) among pathogenic bacteria. despite extensive evidence for exchange of resistance genes from retrospective sequence analyses, experimental evidence for the evolutionary benefits of bacterial recombination is scarce. we compared the evolution of mdr between populations of acinetobacter baylyi in which we manipulated both the recombination rate and the initial diversity of strains with ... | 2011 | 22048956 |
| diverse dna damage responses in acinetobacter include the capacity for dna damage-induced mutagenesis in the opportunistic pathogens acinetobacter baumannii and acinetobacter ursingii. | error-prone and error-free dna damage repair responses that are induced in most bacteria after exposure to various chemicals, antibiotics, or radiation sources were surveyed across the acinetobacter genus. the error prone sos mutagenesis response occurs when dna damage induces a cell's umudc- or dinp-encoded error-prone polymerases. the model acinetobacter baylyi strain adp1 possesses an unusual, regulatory umud allele (umudab) with an extended 5' region, and only incomplete fragments of umuc. d ... | 2011 | 22117008 |
| comparative analysis of the complete genome of an acinetobacter calcoaceticus strain adapted to a phenol-polluted environment. | the complete genome sequence of acinetobacter calcoaceticus phea-2, a non-pathogenic phenol-degrading bacterium previously isolated from industrial wastewater of an oil refinery in china, has been established. this is the first sequence of an a. calcoaceticus strain. we report here a comparative genomic analysis of phea-2 with two other acinetobacter species having different lifestyles, acinetobacter baumannii aye, a pathogenic human-adapted strain, and acinetobacter baylyi adp1, a soil-living s ... | 2011 | 22027104 |
| Synthesis of FAEEs from glycerol in engineered Saccharomyces cerevisiae using endogenously produced ethanol by heterologous expression of an unspecific bacterial acyltransferase. | The high price of petroleum-based diesel fuel has led to the development of alternative fuels, such as ethanol. Saccharomyces cerevisiae was metabolically engineered to utilize glycerol as a substrate for ethanol production. For the synthesis of fatty acid ethyl esters (FAEEs) by engineered S. cerevisiae that utilize glycerol as substrate, heterologous expression of an unspecific acyltransferase from Acinetobacter baylyi with glycerol utilizing genes was established. As a result, the engineered ... | 2012 | 21858787 |
| Expression Vectors for Acinetobacter baylyi ADP1. | Acinetobacter baylyi ADP1 is naturally competent and proficient at homologous recombination, so it can be transformed without restriction digests or ligation reactions. Expression vectors for this system, however, are not yet widely available. Here we describe the construction and characterization of inducible expression vectors that replicate as plasmids in A. baylyi or integrate into a nonessential part of its chromosome. These tools will facilitate the engineering of genes and genomes in this ... | 2012 | 22020504 |
| quantification of chemotaxis-related alkane accumulation in acinetobacter baylyi using raman microspectroscopy. | alkanes are one of the most widespread contaminants in the natural environment, primarily as a consequence of biological synthesis and oil spills. many indigenous microbes metabolize alkanes, and the chemotaxis and accumulation in some strains has been identified. for the first time, we apply raman microspectroscopy to identify such chemotaxis-related affinity, and quantify the alkane concentrations via spectral alterations. raman spectral alterations were only found for the alkane chemo-attract ... | 2017 | 28256129 |
| costs and benefits of natural transformation in acinetobacter baylyi. | natural transformation enables acquisition of adaptive traits and drives genome evolution in prokaryotes. yet, the selective forces responsible for the evolution and maintenance of natural transformation remain elusive since taken-up dna has also been hypothesized to provide benefits such as nutrients or templates for dna repair to individual cells. | 2017 | 28202049 |