| characterization of the bacterial community in a biotrickling filter treating high loads of h(2)s by molecular biology tools. | the diversity and spatial distribution of bacteria in a lab-scale biotrickling filter treating high loads of hydrogen sulfide (h(2)s) were investigated. diversity and community structure were studied by terminal-restriction fragment length polymorphism (t-rflp). a 16s rrna gene clone library was established. near full-length 16s rrna gene sequences were obtained, and clones were clustered into 24 operational taxonomic units (otus). nearly 74% and 26% of the clones were affiliated with the phyla ... | 2009 | 19380998 |
| bacterial community analysis of a gas-phase biotrickling filter for biogas mimics desulfurization through the rrna approach. | the bacterial composition of a lab-scale biotrickling filter (btf) treating high loads of h(2)s was investigated by the rrna approach. two 16s rrna gene clone libraries were established 42 and 189 d after reactor startup, while fluorescent in-situ hybridization (fish) with dna probes was performed throughout 260d of reactor operation. diversity, community structure and metamorphosis were studied from reactor startup to fully-established pseudo-steady state operation at near neutral ph and at an ... | 2010 | 20554311 |
| transformation of iron sulfide to greigite by nitrite produced by oil field bacteria. | nitrate, injected into oil fields, can oxidize sulfide formed by sulfate-reducing bacteria (srb) through the action of nitrate-reducing sulfide-oxidizing bacteria (nr-sob). when reservoir rock contains siderite (feco(3)), the sulfide formed is immobilized as iron sulfide minerals, e.g. mackinawite (fes). the aim of our study was to determine the extent to which oil field nr-sob can oxidize or transform fes. because no nr-sob capable of growth with fes were isolated, the well-characterized oil fi ... | 2009 | 19290520 |
| epsilonproteobacteria represent the major portion of chemoautotrophic bacteria in sulfidic waters of pelagic redoxclines of the baltic and black seas. | recent studies have indicated that chemoautotrophic epsilonproteobacteria might play an important role, especially as anaerobic or microaerophilic dark co(2)-fixing organisms, in marine pelagic redoxclines. however, knowledge of their distribution and abundance as actively co(2)-fixing microorganisms in pelagic redoxclines is still deficient. we determined the contribution of epsilonproteobacteria to dark co(2) fixation in the sulfidic areas of central baltic sea and black sea redoxclines by com ... | 2008 | 18952879 |
| 13c-isotope analyses reveal that chemolithoautotrophic gamma- and epsilonproteobacteria feed a microbial food web in a pelagic redoxcline of the central baltic sea. | marine pelagic redoxclines are zones of high dark co(2) fixation rates, which can correspond up to 30% of the surface primary production. however, despite this significant contribution to the pelagic carbon cycle, the identity of most chemolithoautotrophic organisms is still unknown. therefore, the aim of this study was to directly link the dark co(2) fixation capacity of a pelagic redoxcline in the central baltic sea (landsort deep) with the identity of the main chemolithoautotrophs involved. o ... | 2009 | 18793316 |
| the effect of long-term nitrate treatment on srb activity, corrosion rate and bacterial community composition in offshore water injection systems. | biogenic production of hydrogen sulphide (h(2)s) is a problem for the oil industry as it leads to corrosion and reservoir souring. continuous injection of a low nitrate concentration (0.25-0.33 mm) replaced glutaraldehyde as corrosion and souring control at the veslefrikk and gullfaks oil field (north sea) in 1999. the response to nitrate treatment was a rapid reduction in number and activity of sulphate-reducing bacteria (srb) in the water injection system biofilm at both fields. the present lo ... | 2008 | 18752014 |
| genome of the epsilonproteobacterial chemolithoautotroph sulfurimonas denitrificans. | sulfur-oxidizing epsilonproteobacteria are common in a variety of sulfidogenic environments. these autotrophic and mixotrophic sulfur-oxidizing bacteria are believed to contribute substantially to the oxidative portion of the global sulfur cycle. in order to better understand the ecology and roles of sulfur-oxidizing epsilonproteobacteria, in particular those of the widespread genus sulfurimonas, in biogeochemical cycles, the genome of sulfurimonas denitrificans dsm1251 was sequenced. this genom ... | 2008 | 18065616 |
| quantitative distributions of epsilonproteobacteria and a sulfurimonas subgroup in pelagic redoxclines of the central baltic sea. | members of the class epsilonproteobacteria are known to be of major importance in biogeochemical processes at oxic-anoxic interfaces. in pelagic redoxclines of the central baltic sea, an uncultured epsilonproteobacterium related to sulfurimonas denitrificans was proposed to play a key role in chemolithotrophic denitrification (i. brettar, m. labrenz, s. flavier, j. bötel, h. kuosa, r. christen, and m. g. höfle, appl. environ. microbiol. 72:1364-1372, 2006). in order to determine the abundance, a ... | 2007 | 17921285 |
| sulfurimonas paralvinellae sp. nov., a novel mesophilic, hydrogen- and sulfur-oxidizing chemolithoautotroph within the epsilonproteobacteria isolated from a deep-sea hydrothermal vent polychaete nest, reclassification of thiomicrospira denitrificans as sulfurimonas denitrificans comb. nov. and emended description of the genus sulfurimonas. | a novel mesophilic bacterium, strain go25(t), was isolated from a nest of hydrothermal vent polychaetes, paralvinella sp., at the iheya north field in the mid-okinawa trough. cells were motile short rods with a single polar flagellum. growth was observed between 4 and 35 degrees c (optimum 30 degrees c; 13-16 h doubling time) and between ph 5.4 and 8.6 (optimum ph 6.1). the isolate was a facultatively anaerobic chemolithoautotroph capable of growth using molecular hydrogen, elemental sulfur or t ... | 2006 | 16901999 |
| distribution, phylogenetic diversity and physiological characteristics of epsilon-proteobacteria in a deep-sea hydrothermal field. | epsilon-proteobacteria is increasingly recognized as an ecologically significant group of bacteria, particularly in deep-sea hydrothermal environments. in this study, we studied the spatial distribution, diversity and physiological characteristics of the epsilon-proteobacteria in various microbial habitats in the vicinity of a deep-sea hydrothermal vent occurring in the iheya north field in the mid-okinawa trough, by using culture-dependent and -independent approaches. the habitats studied were ... | 2005 | 16156735 |
| sulfurimonas autotrophica gen. nov., sp. nov., a novel sulfur-oxidizing epsilon-proteobacterium isolated from hydrothermal sediments in the mid-okinawa trough. | a novel mesophilic, sulfur- and thiosulfate-oxidizing bacterium, strain ok10(t), was isolated from deep-sea sediments at the hatoma knoll in the mid-okinawa trough hydrothermal field. cells of strain ok10(t) were short rods, each being motile by means of a single polar flagellum. the isolate grew at 10-40 degrees c (optimum 25 degrees c) and ph 4.5-9.0 (optimum ph 6.5). it grew chemolithoautotrophically with elemental sulfur, sulfide and thiosulfate as sole electron donors and oxygen as electron ... | 2003 | 14657107 |
| Genome and physiology of a model Epsilonproteobacterium responsible for sulfide detoxification in marine oxygen depletion zones. | Eutrophication and global climate change lead to expansion of hypoxia in the ocean, often accompanied by the production of hydrogen sulfide, which is toxic to higher organisms. Chemoautotrophic bacteria are thought to buffer against increased sulfide concentrations by oxidizing hydrogen sulfide before its diffusion to oxygenated surface waters. Model organisms from such environments have not been readily available, which has contributed to a poor understanding of these microbes. We present here ... | 2011 | 22203982 |
| characterization of sulfide-oxidizing microbial mats developed inside a full-scale anaerobic digester employing biological desulfurization. | the microbial mats responsible for biological desulfurization from biogas in a full-scale anaerobic digester were characterized in terms of their structure, as well as their chemical and microbial properties. filament-shaped elemental sulfur 100-500-á++m in length was shown to cover the mats, which cover the entire headspace of the digester. this is the first report on filamentous sulfur production in a non-marine environment. the results of the analysis of the mats suggest that the key players ... | 2011 | 21735263 |
| exploring the composition and diversity of microbial communities at the jan mayen hydrothermal vent field using rna and dna. | dna sequencing technology has proven very valuable for analysing the microbiota of poorly accessible ecosystems such as hydrothermal vents. using a combination of amplicon- and shotgun sequencing of small-subunit ribosomal rna and its gene, we examined the composition and diversity of microbial communities from the recently discovered jan mayen vent field, located on mohn's ridge in the norwegian-greenland sea. the communities were dominated by the epsilonproteobacterial genera sulfurimonas and ... | 2011 | 21627670 |
| the effect of nitrate concentration on sulfide-driven autotrophic denitrification in marine sediment. | the effect of nitrate concentration on denitrification rate, microbial community and byproduct accumulation in sulfide-driven autotrophic denitrification, one of the dominant processes during anoxic marine sediment remediation, was investigated in this study. microorganisms which were phylogenetically closely related with sulfurimonas denitrificans of e-proteobacteria and thiohalomonas denitrificans of ?-proteobacteria were the major autotrophic denitrifiers in this study. nitrate concentration ... | 2011 | 21316076 |
| complete genome sequence of sulfurimonas autotrophica type strain (ok10). | sulfurimonas autotrophica inagaki et al. 2003 is the type species of the genus sulfurimonas. this genus is of interest because of its significant contribution to the global sulfur cycle as it oxidizes sulfur compounds to sulfate and by its apparent habitation of deep-sea hydrothermal and marine sulfidic environments as potential ecological niche. here we describe the features of this organism, together with the complete genome sequence and annotation. this is the second complete genome sequence ... | 2010 | 21304749 |
| diversity of active chemolithoautotrophic prokaryotes in the sulfidic zone of a black sea pelagic redoxcline as determined by rrna-based stable isotope probing. | marine pelagic redoxclines are characterized by pronounced activities of chemolithoautotrophic microorganisms. as evidenced by the high dark co(2) fixation rates measured around the oxic-anoxic interface but also in the upper sulfidic zone, the accordant organisms participate in important biogeochemical transformations. although epsilonproteobacteria have been identified as an important chemoautotrophic group in these environments, detailed species-level information on the identity of actively i ... | 2010 | 20649907 |
| ammonium concentrations in produced waters from a mesothermic oil field subjected to nitrate injection decrease through formation of denitrifying biomass and anammox activity. | community analysis of a mesothermic oil field, subjected to continuous field-wide injection of nitrate to remove sulfide, with denaturing gradient gel electrophoresis (dgge) of pcr-amplified 16s rrna genes indicated the presence of heterotrophic and sulfide-oxidizing, nitrate-reducing bacteria (hnrb and sonrb). these reduce nitrate by dissimilatory nitrate reduction to ammonium (e.g., sulfurospirillum and denitrovibrio) or by denitrification (e.g., sulfurimonas, arcobacter, and thauera). monitor ... | 2010 | 20562276 |
| autotrophic denitrification and its effect on metal speciation during marine sediment remediation. | denitrification-based remediation has been proved as a cost-effective approach for organic removal in sediment. however, little attention has been drawn on the concomitant autotrophic denitrification process and its impacts during such treatment. in this study, a contaminated marine sediment sample was treated with nitrate in a series of experiments to characterize the autotrophic denitrification and its impacts on metal speciation. through treatment, as the consequence of autotrophic denitrific ... | 2009 | 19476962 |
| autotrophic denitrification in nitrate-induced marine sediment remediation and sulfurimonas denitrificans-like bacteria. | a group of autotrophic sulfide-oxidizing denitrifying bacteria was identified from an aged anthropogenic marine sediment treated by nitrate for the removal of recalcitrant organic residues. based on 16s rdna similarity, they were most closely related to sulfurimonas denitrificans which oxidizes sulfide to sulfate using nitrate as electron acceptor. this group of bacteria was one of the eleven operational taxonomy units (otus) identified using a universal primer set for eubacteria, but it was acc ... | 2009 | 19406451 |