| a new potato virus in a new lineage of picorna-like viruses. | on constructing a cdna library for potato, 'contaminating' sequences with a significant identity to apple latent spherical virus (alsv) were found. determination of the remaining genome sequence indicated the presence of a bipartite virus with an rna1 and 2 of 7034 and 3315 nucleotides, respectively, excluding a poly(a)tail. rna1 encodes a single polyprotein (233 kda) and shares highest amino acid identity with alsv at 65%. conserved amino acid motifs typical for helicase, protease and rna-depen ... | 2004 | 15503203 |
| genome segment rna-1 of a flat apple isolate of cherry rasp leaf virus: nucleotide sequence analysis and rt-pcr detection. | the sequence of the rna-1 of a flat apple isolate of cherry rasp leaf virus (crlv-fa) was determined using overlapping cdna fragments. crlv-fa rna-1 consists of 6992 nucleotides (nt), excluding a 3' poly (a) tail. a single open reading frame (orf) consisting of 6705 nt was identified. this orf encodes a putative polyprotein consisting of 2235 amino acid (aa) residues, approximately 249.6 kda. when compared to crlv-pot (potato isolate) rna-1 orf, 2 deletions of 5 aa and 10 aa (total 15 aa) were o ... | 2005 | 15789268 |
| characterization and partial genome sequence of stocky prune virus, a new member of the genus cheravirus. | characterization of a seemingly new spherical virus isolated from severely affected plum trees in south-western france indicated that its divided genome is composed of two single-stranded, polyadenylated rnas of approximately 7.4 and 3.7 kb. its particles are composed of three coat protein subunits of approximately 23, 23.5, and 24.5 kda. partial sequencing of the genomic rnas indicated that this new virus, tentatively named stocky prune virus (stpv), is distantly related to the two sequenced ch ... | 2006 | 16380812 |
| cheravirus and sadwavirus: two unassigned genera of plant positive-sense single-stranded rna viruses formerly considered atypical members of the genus nepovirus (family comoviridae). | the genus nepovirus (family comoviridae) was known both for a good level of homogeneity and for the presence of atypical members. in particular, the atypical members of the genus differed by the number of capsid protein (cp) subunits. while typical nepoviruses have a single cp subunit with three structural domains, atypical nepoviruses have either three small cp subunits, probably corresponding to the three individual domains, or a large and a small subunit, probably containing two and one struc ... | 2007 | 17585366 |
| nucleotide sequence analysis of rna-2 of a flat apple isolate of cherry rasp leaf virus with regions showing greater identity to animal picornaviruses than to related plant viruses. | rna-2 of a flat apple isolate of cherry rasp leaf virus (crlv-fa) appears to consist of 3274 nucleotides, excluding a 3' poly (a) tail. the data supports re-classification of crlv in a new genus in the family comoviridae. a single open reading frame (orf) encoding a putative 108 kda polyprotein was identified. potential protease cleavage sites were identified which would result in the production of a putative movement protein (41 kda), and 3 capsid protein subunits (24, 20, and 22 kda, respectiv ... | 2002 | 12181681 |
| complete genome sequence of arracacha virus b: a novel cheravirus. | the complete genome sequences of rna1 and rna2 of the oca strain of the potato virus arracacha virus b were determined using next-generation sequencing. the rna1 molecule is predicted to encode a 259-kda polyprotein with homology to proteins of the cheraviruses apple latent spherical virus (alsv) and cherry rasp leaf virus (crlv). the rna2 molecule is predicted to encode a 102-kda polyprotein which also has homology to the corresponding protein of alsv and, to a lesser degree, crlv (30 % for rna ... | 2013 | 23192172 |
| complete genome sequence of currant latent virus (genus cheravirus, family secoviridae). | the complete nucleotide sequences of rna1 and rna2 of the holandský červený strain of currant latent virus (culv) were determined using next-generation sequencing. the rna1 is predicted to encode a polyprotein 2124 amino acid long with rdrp motifs. the rna2 is predicted to encode a polyprotein 957 amino acid long with homology to the capsid protein of apple latent spherical virus and cherry rasp leaf virus. phylogenetic analysis confirms that culv is a new distinct member of the genus cheravirus ... | 2016 | 26563315 |
| transmission of nepoviruses by xiphinema americanum-group nematodes. | the transmission of north american nepoviruses by putative species belonging to the xiphinema americanum-group is reviewed. xiphinema americanum sensu stricto, x. californicum, and x. rivesi each transmit cherry rasp leaf (crlv), tobacco ringspot (tobrsv), and tomato ringspot nepovirus (tomrsv), and x. bricolensis is a vector of tomrsv. the apparent lack of specificity in the transmission of north american nepoviruses by x. americanum-group species markedly contrasts with the specific associatio ... | 1993 | 19279778 |