[transmission of pea enation mosaic virus by different subspecies and strains of the foxglove aphid, aulacorthum solani (kaltenbach)]. 19734740803
the peach-potato aphid myzus persicae and the tobacco aphid myzus nicotianae have the same esterase-based mechanisms of insecticide resistance.biochemical and molecular studies have established that in the peach-potato aphid, myzus persicae, insecticide resistance is conferred by amplification of genes encoding the insecticide-detoxifying esterases e4 or fe4. here we report that two insecticide-resistant clones of the closely related tobacco aphid myzus nicotianae have elevated esterases indistinguishable from e4 and fe4 and amplified esterase dna sequences, and flanking regions, with identical restriction maps to the m. persicae genes ...19947894746
molecular characterization and imidacloprid selectivity of nicotinic acetylcholine receptor subunits from the peach-potato aphid myzus persicae.the recent introduction of the chloronicotinyl insecticide imidacloprid, targeting insect nicotinic acetylcholine receptors (nachrs), emphasises the importance of a detailed molecular characterisation of these receptors. we are investigating the molecular diversity of insect nachr subunit genes in an important agricultural pest, the peach-potato aphid myzus persicae. two m. persicae alpha-subunit cdnas, mp alpha1 and mp alpha2, have been cloned previously. here we report the isolation of three n ...199910386991
microsatellite isolation, linkage group identification and determination of recombination frequency in the peach-potato aphid, myzus persicae (sulzer) (hemiptera: aphididae).fifteen polymorphic microsatellite markers were used to establish linkage groups and relative rates of recombination in male and female myzus persicae (sulzer) (hemiptera: aphididae) (peach-potato aphid). we cloned nine markers from m. persicae and for these we report primer sequences and levels of allelic diversity and heterozygosity in four australian m. persicae populations. of the remaining six loci, four loci, also cloned from m. persicae, were obtained from g. malarky (natural history muse ...200111486508
the tomato rme1 locus is required for mi-1-mediated resistance to root-knot nematodes and the potato aphid.the tomato mi-1 gene confers resistance against root-knot nematodes (meloidogyne spp.) and a biotype of the potato aphid (macrosiphum euphorbiae). four mutagenized mi-1/mi-1 tomato populations were generated and screened for altered root-knot nematode resistance. four independent mutants belonging to two phenotypic classes were isolated. one mutant was chosen for further analyzes; rme1 (for resistance to meloidogyne) exhibited levels of infection comparable with those found on susceptible contro ...200111576426
transgenic potatoes with enhanced levels of nematode resistance do not have altered susceptibility to nontarget aphids.cysteine proteinase inhibitors (cystatins) confer resistance to plant-parasitic nematodes when expressed in transgenic plants. the survival and growth of nymphs of the peach-potato aphid, myzus persicae, were adversely affected when cystatins were added to artificial diets. when aphids were clip-caged onto transgenic plants expressing chicken egg white cystatin (cewc) there was no adverse effect on aphid fitness. field populations of aphids on transgenic desiree potatoes, expressing cewc or a mo ...200211972767
tritrophic interactions between transgenic potato expressing snowdrop lectin (gna), an aphid pest (peach-potato aphid; myzus persicae (sulz.) and a beneficial predator (2-spot ladybird; adalia bipunctata l.).tritrophic interactions between transgenic potato expressing the insecticidal lectin from snowdrop (galanthus nivalis agglutinin; gna), an aphid pest, myzus persicae (sulz.), and a beneficial predator, the 2-spot ladybird (adalia bipunctata l.) were investigated. clonal plants expressing gna at 0.1-0.2% total soluble protein in leaves were used. no significant effects on development and survival of ladybird larvae fed on aphids from these transgenic plants were observed, with larval survival in ...200312739890
effects of artificial diet containing gna and gna-expressing potatoes on the development of the aphid parasitoid aphidius ervi haliday (hymenoptera: aphidiidae).aphid parasitoids are important biological control agents. the possibility arises that whilst foraging on insect-resistant transgenic plants, they are themselves at risk from direct and indirect effects of the expression of a transgene used to control the pest species. a liquid artificial diet was successfully used to deliver the snowdrop lectin (galanthus nivalis agglutinin; gna) to the peach-potato aphid, myzus persicae. bioassays utilising artificial diet incorporating gna, and excised leaves ...200112770142
spatial and temporal genetic variability in french populations of the peach-potato aphid, myzus persicae.the peach-potato aphid, myzus persicae (sulzer), has a complex reproductive mode in which some lineages reproduce by continuous parthenogenesis, whereas others reproduce sexually once a year. the climate is thought to act directly on the reproductive mode, because sexual eggs are the only form that can resist frost in cold regions. sexual reproduction necessitates an obligatory host alternation that may result in long-distance dispersal. here, we examined the genetic variability at seven microsa ...200312886281
environmentally related patterns of reproductive modes in the aphid myzus persicae and the predominance of two 'superclones' in victoria, australia.asexual organisms that naturally coexist with sexual relatives may hold the key to understanding the maintenance of sex and recombination, a long-standing problem in evolutionary biology. this situation applies to the peach-potato aphid, myzus persicae, in southeastern australia where cyclical parthenogens form mixed populations with obligate parthenogens. we collected m. persicae from several areas across victoria, genotyped them at seven microsatellite loci and experimentally determined their ...200314629364
rme1 is necessary for mi-1-mediated resistance and acts early in the resistance pathway.the tomato gene mi-1 confers resistance to root-knot nematodes (meloidogyne spp.), potato aphid, and whitefly. using genetic screens, we have isolated a mutant, rme1 (resistance to meloidogyne spp.), compromised in resistance to m. javanica and potato aphid. here, we show that the rme1 mutant is also compromised in resistance to m. incognita, m. arenaria, and whitefly. in addition, using an agrobacterium-mediated transient assay in leaves to express constitutive gain-of-function mutant pto(l205d ...200414714868
activity of aphids associated with lettuce and broccoli in spain and their efficiency as vectors of lettuce mosaic virus.this research sought to identify the aphid virus vector species associated with lettuce and broccoli crops in spain, and to determine their population dynamics and ability to transmit lettuce mosaic virus (lmv). green tile traps and moericke yellow water-pan traps were used to monitor aphid flights during the spring and autumn growing seasons of 2001. aphid species feeding on lettuce were counted weekly. the transmission efficiencies of lmv were determined for the aphid species caught most frequ ...200415036838
unexpected effects of chitinases on the peach-potato aphid (myzus persicae sulzer) when delivered via transgenic potato plants (solanum tuberosum linné) and in vitro.with the aim of producing insect-resistant potato plants, internode explants of solanum tuberosum l. cv. désirée were transformed with an agrobacterium strain c58pmp90 containing an insect (phaedon cochleariae: coleoptera, chrysomelidae) chitinase gene and the neomycin phosphotransferase (nptii) gene as selectable marker, both under the control of the viral camv 35s promoter. three transformed potato lines (ch3, ch5 and ch25) exhibiting the highest chitinolytic activities were selected for feedi ...200515865049
response of economically important aphids to components of hemizygia petiolata essential oil.the essential oil of hemizygia petiolata ashby (lamiaceae) contains high levels (>70%) of the sesquiterpene (e)-beta-farnesene, the alarm pheromone for many economically important aphid species. in order to test the suitability of h. petiolata oil as a source of (e)-beta-farnesene for use in new integrated aphid control strategies, behavioural responses of pest aphid species were studied in laboratory and field experiments. in alarm pheromone assays the peach-potato aphid, myzus persicae sulzer, ...200516059962
aphid parasitoid complex in potato in the context of ipm in belgium.parasitic hymenoptera of potato aphids were collected and identified in 2000 and 2001 in 11 potato fields in belgium. nine aphidiidae species, parasiting seven aphid species, were found: aphidius ervi, aphidius matricariae, aphidius picipes, binodoxys angelicae, diaeretiella rapae, praon abjectum, praon gallicium, praon volucre and toxares deltiger. a. ervi and a. picipes were the dominant and sub-dominant species, with 54% and 28% of the primary parasite collected, respectively. both species an ...200516628888
mi-1-mediated aphid resistance involves salicylic acid and mitogen-activated protein kinase signaling cascades.the tomato mi-1 gene confers resistance to root-knot nematodes (meloidogyne spp.), potato aphids (macrosiphum eluphorbiae), and whiteflies (bemisia tabaci and b. tabaci biotype b). resistance to potato aphid is developmentally regulated and is not associated with induction of a hypersensitive response. the nahg transgene that eliminates endogenous salicylic acid (sa) was used to test the role of the sa signaling pathway in the resistance mediated by mi-1 to potato aphids. aphids survived longer ...200616776299
cytochrome c oxidase mrna as an internal control for detection of potato virus y and potato leafroll virus from single aphids by a co-amplification rt-pcr assay.using cytochrome c oxidase subunit 1 (cox1) mrna as the internal control, a triplex reverse transcription-polymerase chain reaction (rt-pcr) for detection of potato virus y (pvy) and potato leafroll virus (plrv) with co-amplification of cox1 from single specimens of various aphid species has been developed. partial length cdna of cox1 from green peach aphid, myzus persicae (sulzer), potato aphid, macrosiphum euphorbiae (thomas), buckthorn aphid, aphis nasturtii (kaltenbach), and pea aphid, acyrt ...200617010449
covariance of phenotypically plastic traits induces an adaptive shift in host selection behaviour.flexibility in adult body size allows generalist parasitoids to use many host species at a cost of producing a range of adult sizes. consequently, host selection behaviour must also maintain a level of flexibility as adult size is related to capture efficiency. in the present study, we investigated covariance of two plastic traits--size at pupation and host size selection behaviour-using aphidius ervi reared on either acyrthosiphon pisum or aulacorthum solani, generating females of disparate siz ...200617015365
the efficacy of a novel insecticidal protein, allium sativum leaf lectin (asal), against homopteran insects monitored in transgenic tobacco.the homopteran group of polyphagous sucking insect pests causes severe damage to many economically important plants including tobacco. allium sativum leaf lectin (asal), a mannose-binding 25-kda homodimeric protein, has recently been found to be antagonistic to various sucking insects in the homopteran group through artificial diet bioassay experiments. the present study describes, for the first time, the expression of the asal coding sequence under the control of the cauliflower mosaic virus (c ...200517147631
effect of mixed viral infections (potato virus y-potato leafroll virus) on biology and preference of vectors myzus persicae and macrosiphum euphorbiae (hemiptera: aphididae).mixed viral infections of heterologous viruses such as potato virus y (family potyviridae, genus potyvirus, pvy) and potato leafroll virus (family luteoviridae, genus polerovirus, plrv) are a regular occurrence in idaho's potato, solanum tuberosum (l.), cropping systems. an increased number of plant samples from idaho's potato fields over the past 2 yr has serologically tested positive for both pvy and plrv via double antibody sandwich enzyme-linked immunosorbent assay (das-elisa) and exhibited ...200717598521
strong parasitoid-mediated selection in experimental populations of aphids.clonal diversity in asexual populations may be maintained if different clones are favoured under different environmental conditions. for aphids, parasitoids are an important variable of the biotic environment. to test whether parasitoids can mediate selection among host clones, we used experimental populations consisting of 10 clones of the peach-potato aphid, myzus persicae, and allowed them to evolve for several generations either without parasitoids or in the presence of two species of parasi ...200717785263
effect of an alternate weed host, hairy nightshade, solanum sarrachoides, on the biology of the two most important potato leafroll virus (luteoviridae: polerovirus) vectors, myzus persicae and macrosiphum euphorbiae (aphididae: homoptera).hairy nightshade, solanum sarrachoides (sendtner), is a ubiquitous weed in potato agro-ecosystems and nonagricultural lands of southeastern idaho and the pacific northwest. this weed increases the complexity of the potato leafroll virus (plrv) (luteoviridae: polervirus)-potato pathosystem by serving as aphid and virus reservoir. previous field studies showed higher densities of green peach aphid, myzus persicae (sulzer), and potato aphid, macrosiphum euphorbiae (thomas), the two most important v ...200818419933
plum pox in north america: identification of aphid vectors and a potential role for fruit in virus spread.abstract thirteen aphid species were tested for their ability to transmit pennsylvania isolates of plum pox virus (ppv) collected in columbia (penn-3), franklin (penn-4), and york (penn-7) counties, pa. four species, aphis fabae, a. spiraecola, brachycaudus persicae, and myzus persicae, consistently transmitted ppv in preliminary transmission tests. two species, metopolophium dirhodum and rhopalosiphum padi, were occasional inefficient vectors. toxoptera citricida, from florida, also was an effe ...200418943108
the n-terminal region of the readthrough domain is closely related to aphid vector specificity of soybean dwarf virus.abstract it has been speculated that the n-terminal half of the readthrough domain (rtd) encoded by open reading frame 5 of soybean dwarf virus (sbdv) is related to the vector specificity. to further investigate this hypothesis, transmissibility via aphids was tested on 17 sbdv isolates and comparisons of the deduced amino acid sequences of the coat protein (cp) and other proteins encoded by the rtd were made between these isolates. isolates were distinguished into four strains: ys, causing yell ...200318943620
identification, characterization, and relatedness of luteovirus isolates from forage legumes.abstract virus isolates from forage legumes collected from eight different states were identified as luteoviruses closely related to soybean dwarf luteovirus dwarfing (sbdv-d) and yellowing (sbdv-y) described in japan. all isolates produced reddened leaf margins in subterranean clover and were transmitted in a persistent manner by acrythosiphon pisum, but not by aulacorthum solani. specific monoclonal antibodies raised against sbdv-y were differentially reactive with endemic isolates. immunoblot ...199918944749
tracking the global dispersal of a cosmopolitan insect pest, the peach potato commerce and human transportation are responsible for the range expansion of various insect pests such as the plant sucking aphids. high resolution dna markers provide the opportunity to examine the genetic structure of aphid populations, identify aphid genotypes and infer their evolutionary history and routes of expansion which is of value in developing management strategies. one of the most widespread aphid species is the peach-potato aphid myzus persicae, which is considered as a serio ...200919432979
a strain of the bacterial symbiont regiella insecticola protects aphids against parasitoids.aphids commonly harbour facultative bacterial endosymbionts and may benefit from their presence through increased resistance to parasitoids. this has been demonstrated for hamiltonella defensa and serratia symbiotica, while a third common endosymbiont, regiella insecticola, did not provide such protection. however, this symbiont was recently detected in a highly resistant clone of the peach-potato aphid, myzus persicae, from australia. to test if resistance was indeed conferred by the endosymbio ...201019776066
reproductive performance of asexual clones of the peach-potato aphid, ( myzus persicae, homoptera: aphididae), colonising scotland in relation to host plant and field ecology.the population of peach-potato aphid, myzus persicae (sulzer), in scotland comprises large numbers of a few superclones with much smaller numbers of other clones, and the reason for their differential success has yet to be elucidated. in the current study, the reproduction of lineages derived from these clones was measured by counting the numbers of offspring produced by a one-day-old nymph after 15 days. this was measured on four plant species, including local agricultural hosts and at two diff ...201019941675
metabolic enzyme(s) confer imidacloprid resistance in a clone of myzus persicae (sulzer) (hemiptera: aphididae) from greece.previous studies have reported varying levels of resistance against imidacloprid in several insect species, including populations of the peach-potato aphid, myzus persicae (sulzer). these cases of resistance have been attributed to either target-site resistance or enhanced detoxification. in this study, a clone of m. persicae originating from greece revealed a 60-fold resistance factor to imidacloprid.201019950404
tritrophic interactions among macrosiphum euphorbiae aphids, their host plants and endosymbionts: investigation by a proteomic approach.the mi-1.2 gene in tomato confers resistance against certain clones of the potato aphid (macrosiphum euphorbiae). this study used 2d-dige coupled with protein identification by maldi-tof-ms to compare the proteome patterns of avirulent and semivirulent potato aphids and their bacterial endosymbionts on resistant (mi-1.2+) and susceptible (mi-1.2-) tomato lines. avirulent aphids had low survival on resistant plants, whereas the semivirulent clone could colonize these plants. eighty-two protein sp ...201019962988
host-adapted parasitoids in biological control: does source matter?it has been hypothesized that the success of a biological control introduction is, in part, dependent on the ability of the control agent to become established in its new environment or to its new population of hosts through local adaptation. despite this, few studies have investigated the influence of the recent coevolutionary history of pest species and natural enemies on the efficacy of biological control agents, especially for agents that are mass-reared for release in agriculture. we invest ...201020349844
[quality of different aphids species as hosts for the parasitoid aphidius ervi haliday (hymenoptera: braconidae: aphidiinae)].the suitability of macrosiphum euphorbiae (thomas), aulacorthum solani (kaltenbach) and acyrthosiphon kondoi shinji (hemiptera: aphididae) as hosts for the aphid parasitoid aphidius ervi haliday was evaluated by assessing host size, host preference, and host quality. tests were carried out in an environmental chamber at 22±1ºc, 70±10% rh and 12h photophase. replicates (11) consisted of one 24h-old mated female of a. ervi without a previous oviposition experience. female was released into a petri ...201021120377
the receptor-like kinase slserk1 is required for mi-1-mediated resistance to potato aphids in tomato.the plant receptor-like kinase somatic embryogenesis receptor kinase 3 (serk3)/brassinosteroid insensitive 1-associated kinase 1 (bak1) is required for pathogen-associated molecular pattern (pamp)-triggered immunity (pti). here we show that a distinct member of the serk family, serk1, is required for the full functioning of mi-1, a nucleotide binding leucine-rich repeat (nb-lrr) resistance protein. mi-1 confers resistance to meloidogyne spp. (root-knot nematodes, rkns) and three phloem-feeding i ...201121481032
slwrky70 is required for mi-1-mediated resistance to aphids and nematodes in tomato.plant resistance (r) gene-mediated defense responses against biotic stresses include vast transcriptional reprogramming. in several plant-pathogen systems, members of the wrky family of transcription factors have been demonstrated to act as both positive and negative regulators of plant defense transcriptional networks. to identify the possible roles of tomato (solanum lycopersicum) wrky transcription factors in defense mediated by the r gene mi-1 against potato aphid, macrosiphum euphorbiae, an ...201121898085
unpredicted impacts of insect endosymbionts on interactions between soil organisms, plants and aphids.ecologically significant symbiotic associations are frequently studied in isolation, but such studies of two-way interactions cannot always predict the responses of organisms in a community setting. to explore this issue, we adopt a community approach to examine the role of plant-microbial and insect-microbial symbioses in modulating a plant-herbivore interaction. potato plants were grown under glass in controlled conditions and subjected to feeding from the potato aphid macrosiphum euphorbiae. ...201323926148
groel from the endosymbiont buchnera aphidicola betrays the aphid by triggering plant defense.aphids are sap-feeding plant pests and harbor the endosymbiont buchnera aphidicola, which is essential for their fecundity and survival. during plant penetration and feeding, aphids secrete saliva that contains proteins predicted to alter plant defenses and metabolism. plants recognize microbe-associated molecular patterns and induce pattern-triggered immunity (pti). no aphid-associated molecular pattern has yet been identified. by mass spectrometry, we identified in saliva from potato aphids (m ...201424927572
the potato aphid salivary effector me47 is a glutathione-s-transferase involved in modifying plant responses to aphid infestation.polyphagous aphid pests cause considerable economic damage to crop plants, primarily through the depletion of photoassimilates and transfer of viruses. the potato aphid (macrosiphum euphorbiae) is a notable pest of solanaceous crops, however, the molecular mechanisms that underpin the ability to colonize these hosts are unknown. it has recently been demonstrated that like other aphid species, m. euphorbiae injects a battery of salivary proteins into host plants during feeding. it is speculated t ...201627536306
the ethylene response factor pti5 contributes to potato aphid resistance in tomato independent of ethylene signalling.ethylene response factors (erfs) comprise a large family of transcription factors that regulate numerous biological processes including growth, development, and response to environmental stresses. here, we report that pti5, an erf in tomato [solanum lycopersicum (linnaeus)] was transcriptionally upregulated in response to the potato aphid macrosiphum euphorbiae (thomas), and contributed to plant defences that limited the population growth of this phloem-feeding insect. virus-induced gene silenci ...201425504643
loss of function of fatty acid desaturase7 in tomato enhances basal aphid resistance in a salicylate-dependent manner.we report here that disruption of function of the ω-3 fatty acid desaturase7 (fad7) enhances plant defenses against aphids. the suppressor of prosystemin-mediated responses2 (spr2) mutation in tomato (solanum lycopersicum), which eliminates the function of fad7, reduces the settling behavior, survival, and fecundity of the potato aphid (macrosiphum euphorbiae). likewise, the antisense suppression of lefad7 expression in wild-type tomato plants reduces aphid infestations. aphid resistance in the ...201222291202
in planta expression or delivery of potato aphid macrosiphum euphorbiae effectors me10 and me23 enhances aphid fecundity.the interactions between aphids and their host plants seem to be analogous to those of plant-microbial pathogens. unlike microbial pathogen effectors, little is known about aphid effectors and their ability to interfere with host immunity. to date, only three functional aphid effectors have been reported. to identify potato aphid (macrosiphum euphorbiae) effectors, we developed a salivary gland transcriptome using illumina technology. we generated 85 million illumina reads from salivary glands a ...201323194342
alkyl-substituted δ-lactones derived from dihydrojasmone and their stereoselective fungi-mediated conversion: production of new antifeedant agents.a chemoenzymatic method was applied to obtain optically pure alkyl-substituted δ-lactones. first, chemical baeyer-villiger oxidation of dihydrojasmone (1) was carried out, affording two new alkyl-substituted δ-lactones: 3,4-dihydro-5-methyl-6-pentyl-2h-pyran-2-one (2) and 5-methyl-6-pentyl-1,13-dioxabicyclo[4.1.0]heptan-2-one (3). in the next step, fungal strains were investigated as biocatalysts to enantioselective conversion of δ-lactones (2) and (3). the fungal cultures: fusarium culmorum am1 ...201627649116
biotransformation of bicyclic halolactones with a methyl group in the cyclohexane ring into hydroxylactones and their biological activity.the aim of this study was the chemical synthesis of a series of halo- and unsaturated lactones, as well as their microbial transformation products. finally some of their biological activities were assessed. three bicyclic halolactones with a methyl group in the cyclohexane ring were obtained from the corresponding γ,δ-unsaturated ester during a two-step synthesis. these lactones were subjected to screening biotransformation using twenty two fungal strains. these strains were tested on their abil ...201627809258
the tomato mi-1 gene confers resistance to both root-knot nematodes and potato aphids.mi-1, a lycopersicon peruvianum gene conferring resistance to the agricultural pests, root-knot nematodes, and introgressed into tomato, has been cloned using a selective restriction fragment amplification based strategy. complementation analysis of a susceptible tomato line with a 100 kb cosmid array yielded a single cosmid clone capable of conferring resistance both to the root-knot nematode meloidogyne incognita and to an unrelated pathogen, the potato aphid macrosiphum euphorbiae. this resis ...19989853621
plant resistance to aphid feeding: behavioral, physiological, genetic and molecular cues regulate aphid host selection and feeding.aphids damage major world food and fiber crops through direct feeding and transmission of plant viruses. fortunately, the development of many aphid-resistant crop plants has provided both ecological and economic benefits to food production. plant characters governing aphid host selection often dictate eventual plant resistance or susceptibility to aphid herbivory, and these phenotypic characters have been successfully used to map aphid resistance genes. aphid resistance is often inherited as a d ...201424282145
heterologous expression of the mi-1.2 gene from tomato confers resistance against nematodes but not aphids in eggplant.the mi-1.2 gene in tomato (solanum lycopersicum) is a member of the nucleotide-binding leucine-rich repeat (nblrr) class of plant resistance genes, and confers resistance against root-knot nematodes (meloidogyne spp.), the potato aphid (macrosiphum euphorbiae), and the sweet potato whitefly (bemisia tabaci). mi-1.2 mediates a rapid local defensive response at the site of infection, although the signaling and defensive pathways required for resistance are largely unknown. in this study, eggplant ...200616610741
aphid-induced defense responses in mi-1-mediated compatible and incompatible tomato interactions.the tomato mi-1 gene confers resistance to three species of root-knot nematode and potato aphid. we studied changes in expression of jasmonic acid (ja)- and salicylic acid (sa)-dependent defense genes in response to potato and green peach aphids. we determined changes in three pr proteins, lipoxygenase and proteinase inhibitors i and ii transcripts, locally and systemically in both compatible and incompatible interactions in tomato. transcripts for pr-1 were detected earlier and accumulated to h ...200312906114
the root-knot nematode resistance gene mi-1.2 of tomato is responsible for resistance against the whitefly bemisia tabaci.the tomato gene mi-1.2 confers resistance against root-knot nematodes and some isolates of potato aphid. resistance to the whitefly bemisia tabaci previously has been observed in mi-bearing commercial tomato cultivars, suggesting that mi, or a closely linked gene, is responsible for the resistance. the response of two biotypes of b. tabaci to tomato carrying the cloned mi was compared with that of the isogenic untransformed tomato line moneymaker. our results indicate that mi-1.2 is responsible ...200312848430
mi-1.2 transcripts accumulate ubiquitously in resistant lycopersicon esculentum.the tomato mi-1.2 gene confers resistance against both root-knot nematodes and the potato aphid. plants are resistant to nematodes early in root development. however, plants as old as 4 weeks are susceptible to aphid infestation. we monitored mi-1.2 expression at the transcriptional level in resistant (mi/mi) and susceptible (mi/mi) tomato cultivars by means of rt-pcr. mi-1.2 transcripts accumulated in seeds, roots, stems, leaves, flowers, and green fruits of uninfected 10-week-old resistant pla ...200119266006
stopped in its tracks: how λ-cyhalothrin can break the aphid transmission of a potato potyvirus.pyrethroids are one of the most widespread and commonly used classes of insecticide and are used in multiple roles, including protecting potato crops from virus vector aphids. resistance in some genotypes of a few species is now widespread, but most species remain susceptible. the rate of virus transmission by two genotypes of the peach potato aphid, myzus persicae, fed on potato virus y (pvy)-infected leaves of potato treated with the pyrethroid λ-cyhalothrin was evaluated.201525523839
synthesis and antifeedant activity of racemic and optically active hydroxy lactones with the p-menthane system.two racemic and two enantiomeric pairs of new δ-hydroxy-γ-lactones based on the p-menthane system were prepared from racemic and optically active cis- and trans-piperitols. the johnson-claisen rearrangement of the piperitols, epoxidation of the γδ-unsaturated esters, and acidic lactonization of the epoxy esters were described. the structures of the compounds were confirmed spectroscopically. the antifeedant activities of the hydroxy lactones and racemic piperitone were evaluated against three in ...201526132506
glutathione s-transferases of aulacorthum solani and acyrthosiphon pisum: partial purification and characterization.glutathione s-transferases (gst) play an important role in the detoxification of many substances including allelochemicals from plants. brassicaceae plants contain glucosinolates and emit volatile isothiocyanates which affect the gst system. a comparison of the gst of two aphid species, the generalist aulacorthum solani found on brassicaceae and the fabaceae specialist acyrthosiphon pisum, was made to try to explain their respective feeding behaviour. differences of gst were determined among the ...200111337260
characterization of non-ltr retrotransposable tras elements in the aphids acyrthosiphon pisum and myzus persicae (aphididae, hemiptera).a non-ltr tras retrotransposon (identified as trasap1) has been amplified in the pea aphid acyrthosiphon pisum and its presence has been assessed also in the peach potato aphid myzus persicae. this tras element possesses 2 overlapping orfs (a gag-orf1 and a pol-orf2 containing the reverse transcriptase and the endonuclease domains) that show a similarity ranging from 40% to 48% to proteins coded by other tras elements identified in insects (including the beetle tribolium castaneum and the moth b ...201323530141
genomic and cytogenetic localization of the carotenoid genes in the aphid published in the scientific literature suggests a possible link between chromosomal rearrangements involving autosomes 1 and 3 and the presence of red morphs in the peach-potato aphid myzus persicae (sulzer). in order to begin a study of this relationship, we analysed the genomic and chromosomal location of genes involved in carotenoid biosynthesis in m. persicae and the pea aphid, acyrthosiphon pisum (harris), since carotenoids are the basis of the colour in many aphid species. genomic ana ...201627585067
fine mapping of foxglove aphid (aulacorthum solani) resistance gene raso1 in soybean and its effect on tolerance to soybean dwarf virus transmitted by foxglove aphid.soybean dwarf virus (sbdv) causes serious dwarfing, yellowing and sterility in soybean (glycine max). the soybean cv. adams is tolerant to sbdv infection in the field and exhibits antibiosis to foxglove aphid (aulacorthum solani), which transmits sbdv. this antibiosis (termed "aphid resistance") is required for tolerance to sbdv in the field in segregated progenies of adams. a major quantitative trait locus, raso1, is reported for foxglove aphid resistance. our objectives were to fine map raso1 ...201223136500
effects of manduca sexta allatostatin and an analogue on the peach-potato aphid myzus persicae (hemiptera: aphididae) and degradation by enzymes in the aphid gut.the oral toxicity of the c-type allatostatin, manduca sexta allatostatin (manse-as) and the analogue δr³δr⁵manse-as, where r residues were replaced by their d-isomers, were tested against the peach-potato aphid myzus persicae by incorporation into an artificial diet. both peptides had significant dose-dependent effects on mortality, growth, and fecundity compared with control insects. the analogue, δr³δr⁵manse-as, had an estimated lc₅₀ of 0.31 µg/µl diet and was more potent than manse-as (estima ...201020936640
genetic variation and covariation of susceptibility to parasitoids in the aphid myzus persicae: no evidence for trade-offs.parasitoids are an important mortality factor for insects. susceptibility to parasitoids should thus be under strong negative selection. nevertheless, ample genetic variation for susceptibility to parasitoids is commonly observed in natural populations, suggesting that trade-offs may constrain the evolution of reduced susceptibility. this can be studied by assessing genetic variation for susceptibility and its covariation with other components of fitness. in a set of 17 clones of the peach potat ...200818270153
expression and purification of arisaema heterophyllum agglutinin in escherichia coli.recombinant arisaema heterophyllum agglutinin (aha) was expressed in escherichia coli as n-terminal his-tagged fusions. after induction with isopropylthio-beta-d-galactoside, the recombinant aha was purified by metal-affinity chromatography. the purified aha protein was incorporated into artificial diet at 0.1% (w/v) concentration in insect bioassay trial and the result showed that artificial diet containing aha could significantly inhibit the growth of the third-instar nymphs of peach potato ap ...200616399011
biological control outcomes using the generalist aphid predator aphidoletes aphidimyza under multi-prey conditions.the aphidophagous midge aphidoletes aphidimyza (diptera: cecidomyiidae) is used in biological control programs against aphids in many crops. short-term trials with this natural enemy demonstrated that that females prefer to oviposit among aphids colonizing the new growth of plants, leading to differential attack rates for aphid species that differ in their within-plant distributions. thus, we hypothesized that biological control efficacy could be compromised when more than one aphid species is p ...201627983620
genetic variation in target-site resistance to pyrethroids and pirimicarb in tunisian populations of the peach potato aphid, myzus persicae (sulzer) (hemiptera: aphididae).we used molecular assays to diagnose resistance to pyrethroids and pirimicarb in samples of myzus persicae from field crops or an insect suction trap in tunisia. genotypes for resistance loci were related to ones for polymorphic microsatellite loci in order to investigate breeding systems and patterns of genetic diversity, and to inform resistance management tactics.201626990432
the conformation of a plasma membrane-localized somatic embryogenesis receptor kinase complex is altered by a potato aphid-derived effector.somatic embryogenesis receptor kinases (serks) are transmembrane receptors involved in plant immunity. tomato (solanum lycopersicum) carries three serk members. one of these, slserk1, is required for mi-1.2-mediated resistance to potato aphids (macrosiphum euphorbiae). mi-1.2 encodes a coiled-coil nucleotide-binding leucine-rich repeat protein that in addition to potato aphids confers resistance to two additional phloem-feeding insects and to root-knot nematodes (meloidogyne spp.). how slserk1 p ...201627208261
pharmacological profiles of recombinant and native insect nicotinic acetylcholine receptors.nicotinic acetylcholine receptors (nachrs) are targets for insect-selective neonicotinoid insecticides exemplified by imidacloprid (imi) and mammalian-selective nicotinoids including nicotine and epibatidine (epi). despite their importance, insect nachrs are poorly understood compared with their vertebrate counterparts. this study characterizes the [(3)h]imi, [(3)h]epi, and [(3)h]alpha-bungarotoxin (alpha-bgt) binding sites in hybrid nachrs consisting of drosophila melanogaster (fruit fly) or my ...200516291090
insect antifeedant potential of xanthohumol, isoxanthohumol, and their derivatives.xanthohumol (14) and isoxanthohumol (6) derived from hop (humulus lupulus l., cannabaceae) and selected chalcone and chromene derivatives, obtained by chemical synthesis, were studied for antifeedant activity against the peach-potato aphid (myzus persicae [sulz.]). the study used also commercially available 4-chromanone (1), flavanone (4), naringenin (5), chromone (7), flavone (8), 7-aminoflavone (9), trans-chalcone (10), and 4-methoxychalcone (12). for chromone derivatives it was observed that ...201526176501
expression and characterization of carboxylesterase e4 gene from peach-potato aphid (myzus persicae) for degradation of carbaryl and abridged carboxylesterase e4 (cbe e4) gene was cloned from the peach-potato aphid, myzus persicae, by reverse transcription-pcr and subcloned into the expression vector pet28b. the abridged cbe e4 gene was successfully expressed in e. coli bl21 (de3). the recombinant cbe e4 hydrolyzed beta-naphthyl acetate and carbaryl by 64% within 2.5 h, malathion by 80% within 1.25 h. however, the hydrolysis of other pesticides (dichlorovos, parathion, pirimicarb and deltamethrin) was not detected.200516132866
does insecticide resistance alone account for the low genetic variability of asexually reproducing populations of the peach-potato aphid myzus persicae?the typical life cycle of aphids includes several parthenogenetic generations and a single sexual generation (cyclical parthenogenesis), but some species or populations are totally asexual (obligate parthenogenesis). genetic variability is generally low in these asexually reproducing populations, that is, few genotypes are spread over large geographic areas. both genetic drift and natural selection are often invoked to account for this low genetic variability. the peach-potato aphid, myzus persi ...200515940274
karyotype rearrangements and telomere analysis in myzuspersicae (hemiptera, aphididae) strains collected on lavandula sp. plants.karyotype analysis of nine strains of the peach-potato aphid myzuspersicae (sulzer, 1776), collected on lavandula sp. plants, evidenced showed that five of them had a standard 2n = 12 karyotype, one possessed a fragmentation of the x chromosome occurring at the telomere opposite to the nor-bearing one and three strains had a chromosome number 2n = 11 due to a non-reciprocal translocation of an autosome a3 onto an a1 chromosome. interestingly, the terminal portion of the autosome a1 involved in t ...201425610541
transgenic plants expressing ω-actx-hv1a and snowdrop lectin (gna) fusion protein show enhanced resistance to aphids.recombinant fusion proteins containing arthropod toxins have been developed as a new class of biopesticides. the recombinant fusion protein hv1a/gna, containing the spider venom toxin ω-actx-hv1a linked to snowdrop lectin (gna) was shown to reduce survival of the peach-potato aphid myzus persicae when delivered in artificial diet, with survival <10% after 8 days exposure to fusion protein at 1 mg/ml. although the fusion protein was rapidly degraded by proteases in the insect, hv1a/gna oral toxic ...201425506351
transgenic-bt potato plant resistance to the colorado potato beetle affect the aphid parasitoid aphidius nigripes.using the biotechnological plant resistance for herbivore control with less reliance on chemicals in integrated pest management (ipm) programs critically depends on predictable interactions with no-target organisms of various trophic levels. plant resistance to insect pests based on recombinant bacillus thuringiensis could interfere with natural enemies of non target pests. performance of the potato aphid parasitoid aphidius nigripes was studied on the 'superior-bt line transgenic for the crylli ...200415759411
aphid specific predators in potato in summer 2003, aphid specific predators populations were sampled in four potato fields located in wallonia. ladybirds, hoverflies and lacewings were the most abundant predators collected. a total of 6 ladybird, 8 syrphid and 2 lacewing species was found. predator species composition and abundance greatly differed from field to field, while aphids populations were more or less similar with a peak of 3 to 5 aphid/potato leaf in mid-july. all chrysopidae sampled belong to the chrysoperla kolthoffi ...200415759406
analysis of spatial patterns at a geographical scale over north-western europe from point-referenced aphid count data.the spatial analysis by distance indices (sadie) technique was developed to evaluate the spatial pattern of point-referenced count data as well as the spatial association between two sets of data sharing the same point locations. this paper presents an analysis of spatial patterns in aphid count data and the association of these data with climate across north-west europe. the paper tests the applicability of the technique to large geographical areas. aggregation and cluster indices were calculat ...200515705214
dna methylation in insects.cytosine dna methylation has been demonstrated in numerous eukaryotic organisms and has been shown to play an important role in human disease. the function of dna methylation has been studied extensively in vertebrates, but establishing its primary role has proved difficult and controversial. analysing methylation in insects has indicated an apparent functional diversity that seems to argue against a strict functional conservation. to investigate this hypothesis, we here assess the data reported ...200415056357
transgenic tobacco expressing pinellia ternata agglutinin confers enhanced resistance to aphids.tobacco leaf discs were transformed with a plasmid, pbipta, containing the selectable marker neomycin phosphotransferase gene (nptii) and pinellia ternata agglutinin gene (pta) via agrobacterium tumefaciens-mediated transformation. thirty-two independent transgenic tobacco plants were regenerated. pcr and southern blot analyses confirmed that the pta gene had integrated into the plant genome and northern blot analysis revealed transgene expression at various levels in transgenic plants. genetic ...200314713200
probiotic effects of beta-glucuronidase on the peach-potato aphid myzus persicae (aphididae).beta-glucuronidase (gus) is a reporter protein commonly expressed in transgenic plants allowing the visualization of the transformed individuals. in our recent work, we showed that consumption of transformed potato plants expressing this gus enzyme improves performance of the phloem feeding aphid myzus persicae. those results led us to the conclusion that the expression of gus in potato plants might be responsible for the probiotic effect measured in feeding aphids. in the present paper, artific ...200314624892
transformation of tobacco with genes encoding helianthus tuberosus agglutinin (hta) confers resistance to peach-potato aphid (myzus persicae).the effects of the hta gene encoding helianthus tuberosus agglutinin (hta) on an insect in the order homoptera were investigated. homologous cdnas of hta-a, hta-b, hta-c and hta-d with camv35s as promoter were introduced into tobacco via agrobacterium tumefaciens. southern blot results showed that the exogenous hta gene was inserted into the genome of host plants, and northern blot analysis confirmed that hta was expressed in transgenic plants. a bioassay with peach-potato aphid (myzus persicae) ...200314601659
incidence of insecticide resistance alleles in sexually-reproducing populations of the peach-potato aphid myzus persicae (hemiptera: aphididae) from southern france.intensive chemical treatments have led to the development of a number of insecticide resistance mechanisms in the peach-potato aphid myzus persicae (sulzer). some of these mechanisms are known to be associated with negative pleiotropic effects (resistance costs). molecular and biochemical methods were used to determine the genotypes or phenotypes associated with four insecticide resistance mechanisms in single aphids from sexually-reproducing populations in southern france. the mechanisms consid ...200312908914
aphid pests of strawberry crops and their parasitoids in france.the pest status of aphids on strawberry crops has changed with time. they were mainly considered with respect to virus transmission. now, they are rather considered as sap-feeders and honeydew producers. as such, low populations regulated by natural enemies can be tolerated and systematic use of insecticides avoided. a survey of the species present in the different areas of production has been undertaken during 1999 and 2000 in france. during this survey, well known species of the strawberry cro ...200112425050
comparison of complete nucleotide sequences of genomic rnas of four soybean dwarf virus strains that differ in their vector specificity and symptom production.soybean dwarf virus (sbdv) is divided into four strains, namely ys, yp, ds and dp. ys and yp cause yellowing in soybeans, while ds and dp cause dwarfing. ys and ds are transmitted by aulacorthum solani, while yp and dp are transmitted by acyrthosiphon pisum. to clarify the taxonomic relationship between the four strains and to classify sbdv into an appropriate genus in the luteoviridae, we determined the complete nucleotide sequences of genomic rnas of four isolates belonging to each of the stra ...200111722012
lactones. 9. synthesis of terpenoid lactones-active insect antifeedants.starting from (+)- and (-)-perillyl alcohols, via claisen rearrangement and iodolactonization, four enantiomeric pairs of gamma-lactones were obtained. the structures of compounds were established by both spectroscopic and crystallographic methods. the lactones were tested for antifeeding activity toward grain storage pests-the granary weevil beetle (sitophilus granarius l.), the khapra beetle (trogoderma granarium ev.), and the confused flour beetle (tribolium confusum duv.). the results of the ...200011052764
a member of the hsp60 gene family from the peach potato aphid, myzus persicae (sulzer.).a novel cdna clone encoding a mitochondrial hsp60 was isolated from a myzus persicae cdna library. the nucleotide sequence consisted of 2348 bp and contained an open reading frame (orf) of 1722 bases. the putative protein encoded by this orf consisted of 574 amino acids and was designated mphsp60. comparison of mphsp60 with other hsp60s found that it was most similar to hsp60 from culicoides variipennis (85.6% similarity). phylogenetic analysis revealed that mphsp60 is clustered together with ot ...200010762429
identification of mutations conferring insecticide-insensitive ache in the cotton-melon aphid, aphis gossypii glover.we have identified two mutations in the ace1 gene of aphis gossypii that are associated with insensitivity of acetylcholinesterase (ache) to carbamate and organophosphate insecticides. the first of these, s431f (equivalent to f331 in torpedo californica), is associated with insensitivity to the carbamate insecticide pirimicarb in a range of a. gossypii clones. the s431f mutation is also found in the peach-potato aphid, myzus persicae (sulzer), and a rapid rflp diagnostic allows the identificatio ...200415373812
pathogenicity of conidia-based preparations of entomopathogenic fungi against the greenhouse pest aphids myzus persicae, aphis gossypii, and aulacorthum solani (hemiptera: aphididae).seeking new isolates of entomopathogenic fungi with greater virulence against greenhouse aphid pests than those currently registered in north america for control of these insects, single-dose screening assays of 44 selected fungal isolates and 4 commercially available strains were conducted against first-instar nymphs of myzus persicae and aphis gossypii. the assays identified a number of beauveria and metarhizium isolates with virulence equal to or greater than that of the commercial strains ag ...201424583227
behavioural avoidance and enhanced dispersal in neonicotinoid-resistant myzus persicae (sulzer).the peach potato aphid myzus persicae is a major agricultural pest capable of transmitting over 100 plant viruses to a wide range of crops. control relies largely upon treatment with neonicotinoid insecticides such as thiamethoxam (tmx). in 2009, a strain denoted frc, which exhibits between 255- and 1679-fold resistance to current neonicotinoids previously linked to metabolic and target site resistance, was discovered in france. dispersal behaviour may potentially further enhance the resistance ...201423483696
aphids (hemiptera, aphididae) on ornamental plants in greenhouses in bulgaria.investigations on the species composition and host range of aphids on ornamental greenhouse plants in bulgaria was conducted over a period of five years, from 2008 to 2012. twenty greenhouses, growing ornamentals for landscaping, plant collections and other purposes were observed. they were located in the regions of sofia, plovdiv, smolyan, pavlikeni, varna and burgas. the total number of collected aphid samples was 279. their composition included 33 aphid species and one subspecies from 13 gene ...201324039530
expression of α-dioxygenase 1 in tomato and arabidopsis contributes to plant defenses against aphids.plant α-dioxygenases (α-dox) are fatty acid-hydroperoxidases that contribute to the synthesis of oxylipins, a diverse group of compounds primarily generated through oxidation of linoleic (la) and linolenic acid (lna). oxylipins are implicated in plant signaling against biotic and abiotic stresses. we report here that the potato aphid (macrosiphum euphorbiae) induces slα-dox1 but not slα-dox2 expression in tomato (solanum lycopersicum). slα-dox1 upregulation by aphids does not require either jasm ...201323634839
aphid-induced accumulation of trehalose in arabidopsis thaliana is systemic and dependent upon aphid density.trehalose is a disaccharide sugar that is now considered to be widely distributed among higher plants. trehalose has been attributed a number of roles, including control of basic plant processes, such as photosynthesis, and conferring tolerance to abiotic stresses, such as desiccation and high salinity. trehalose is also a common storage sugar used by insects. in this study, we used laboratory investigations to examine various aspects of trehalose dynamics in an aphid-host plant system (arabidop ...201323242075
investigations into plant biochemical wound-response pathways involved in the production of aphid-induced plant volatiles.feeding damage to plants by insect herbivores induces the production of plant volatiles, which are attractive to the herbivores natural enemies. little is understood about the plant biochemical pathways involved in aphid-induced plant volatile production. the aphid parasitoid diaeretiella rapae can detect and respond to aphid-induced volatiles produced by arabidopsis thaliana. when given experience of those volatiles, it can learn those cues and can therefore be used as a novel biosensor to dete ...200818583348
the interactions between piperonyl butoxide and e4, a resistance-associated esterase from the peach-potato aphid, myzus persicae sulzer (hemiptera: aphididae).it has been reported previously that piperonyl butoxide (pbo) can inhibit both p450 and esterase activity. although the method by which pbo combines with cytochrome p450 has been identified, the way in which it acts as an esterase inhibitor has not been established. this paper characterises the interactions between pbo and the resistance-associated esterase in myzus persicae, e4.201322969050
insecticide resistance profiles can be misleading in predicting the survival of myzus persicae genotypes on potato crops following the application of different insecticide classes.the accuracy of predicting the survival of insecticide-resistant aphids following the application of commonly used insecticides from the carbamate, the pyrethroid, a mix of the two or the neonicotinoid chemical classes was evaluated in a potato field in scotland. equal proportions of five genotypes of the peach-potato aphid, myzus persicae (sulzer), with none, resistance to dimethyl-carbamates, resistance to pyrethroids or combinations conferring resistance to both chemical classes were released ...201322848031
effect of plant development (age and size) on the mi-1-mediated resistance of tomato to whitefly bemisia tabaci.whitefly, bemisia tabaci, is one of the most important pests of tomato, solanum lycopersicum l. the mi-1 gene mediates tomato resistance to the middle east-asia minor 1 (meam1) and mediterranean (med) species of b. tabaci, three species of root-knot nematodes, meloidogyne spp., and the potato aphid, macrosiphum euphorbiae. tomato seedlings bearing the mi-1 gene are resistant to nematodes soon after germination but resistance to aphids is developmentally regulated; a reliable conclusion about mi- ...201728397642
potato aphid macrosiphum euphorbiae performance is determined by aphid genotype and not mycorrhizal fungi or water availability.intra- and inter-specific variation in plant and insect traits can alter the strength and direction of insect-plant interactions, with outcomes modified by soil biotic and abiotic conditions. we used the potato aphid (macrosiphum euphorbiae thomas) feeding on cultivated s. tuberosum and wild s. berthaulti to study the impact of water availability and plant mutualistic arbuscular mycorrhizal (am) fungi on aphid performance and susceptibility to a parasitoid wasp (aphidius ervi haliday). plants we ...201728213920
activation of defence in sweet pepper, capsicum annum, by cis-jasmone, and its impact on aphid and aphid parasitoid behaviour.two important pests of the sweet pepper, capsicum annuum, are the peach potato aphid, myzus persicae, and the glasshouse potato aphid, aulacorthum solani. current aphid control measures include the use of biological control agents, i.e., parasitic wasps, but with varying levels of success. one option to increase parasitoid efficiency is to activate plant defence. therefore, sweet pepper plants were treated with the naturally occurring plant defence activator cis-jasmone, and its impact upon the ...201222696464
cis-jasmone elicits aphid-induced stress signalling in potatoes.elicitation of plant defense signaling that results in altered emission of volatile organic compounds (vocs) offers opportunities for protecting plants against arthropod pests. in this study, we treated potato, solanum tuberosum l., with the plant defense elicitor cis-jasmone (cj), which induces the emission of defense vocs and thus affects the behavior of herbivores. using chemical analysis, electrophysiological and behavioral assays with the potato-feeding aphid macrosiphum euphorbiae, we show ...201728130741
karyotype variations in italian populations of the peach-potato aphid myzus persicae (hemiptera: aphididae).in this study, we present cytogenetic data regarding 66 myzus persicae strains collected in different regions of italy. together with the most common 2n = 12 karyotype, the results showed different chromosomal rearrangements: 2n = 12 with a1-3 reciprocal translocation, 2n = 13 with a1-3 reciprocal translocation and a3 fission, 2n = 13 with a3 fission, 2n = 13 with a4 fission, 2n = 14 with x and a3 fissions. a 2n = 12-13 chromosomal mosaicism has also been observed. chromosomal aberrations (and i ...201222647317
continuous occurrence of intra-individual chromosome rearrangements in the peach potato aphid, myzus persicae (sulzer) (hemiptera: aphididae).analysis of the holocentric mitotic chromosomes of the peach-potato aphid, myzus persicae (sulzer), from clones labelled 50, 51 and 70 revealed different chromosome numbers, ranging from 12 to 14, even within each embryo, in contrast to the standard karyotype of this species (2n = 12). chromosome length measurements, combined with fluorescent in situ hybridization experiments, showed that the observed chromosomal mosaicisms are due to recurrent fragmentations of chromosomes x, 1 and 3. contrary ...201222644285
a novel virus from macrosiphum euphorbiae with similarities to members of the family flaviviridae.a virus with a large genome was identified in the transcriptome of the potato aphid (macrosiphum euphorbiae) and was named macrosiphum euphorbiae virus 1 (mev-1). the mev-1 genome is 22 780 nt in size, including 3' and 5' non-coding regions, with a single large orf encoding a putative polyprotein of 7333 aa. the c-terminal region of the predicted mev-1 polyprotein contained sequences with similarities to helicase, methyltransferase and rna-dependent rna polymerase (rdrp) motifs, while the n-term ...201626822322
detection of novel qtls for foxglove aphid resistance in soybean.the raso2 , novel qtl for korea biotype foxglove aphid resistance in soybean from pi 366121 was identified on chromosome 7 using goldengate snp microarray. foxglove aphid, aulacorthum solani (kaltenbach), is a hemipteran insect that infects a wide variety of plants worldwide and causes serious yield losses in crops. the objective of this study was to identify the putative qtl for foxglove aphid resistance in wild soybean, pi 366121, (glycine soja sieb. and zucc.). one hundred and forty-one f4-de ...201525904004
potato aphid salivary proteome: enhanced salivation using resorcinol and identification of aphid phosphoproteins.aphids deliver saliva into plants and acquire plant sap for their nourishment using a specialized mouthpart or stylets. aphid saliva is of great importance because it contains effectors that are involved in modulating host defense and metabolism. although profiling aphid salivary glands and identifying secreted proteins have been successfully used, success in direct profiling of aphid saliva have been limited due to scarcity of saliva collected in artificial diets. here we present the use of a n ...201525722084
walking speed adaptation ability of myzus persicae to different temperature conditions.walking speeds were calculated for nine clones of the peach potato aphid myzus persicae collected from three countries along a latitudinal cline of its european distribution from sweden to spain (sweden, uk and spain), and the effects of collection origin and intra and intergenerational acclimation were investigated. walking speeds declined with decreasing temperature, with maximum performance at temperatures closest to acclimation temperature (fastest median walking speed of 5.8 cm min(-1) was ...201222123410
molecular cloning, characterisation and mrna expression of the ryanodine receptor from the peach-potato aphid, myzus persicae.the peach potato aphid, myzus persicae, is one of the most important agricultural pests of temperate climates. it is mainly controlled through the judicious application of insecticides; however, over time, aphids have developed resistance to many insecticidal classes. the recent introduction of synthetic diamide insecticides, with a novel mode of action, potentially offers new tools to control aphid populations. these diamides act on the ryanodine receptor (ryr), a large endoplasmic calcium rele ...201525447916
occurrence of rabl-like telomere clustering in the holocentric chromosomes of the peach potato aphid myzus persicae (hemiptera; aphididae).several studies demonstrated that chromosome anchoring to nuclear structures is involved in the organization of the interphase nucleus. the rabl configuration, a well-studied chromosome organization in the interphase nucleus, has been deeply studied in organisms with monocentric chromosomes but just slightly touched in species with holocentric chromosomes. in the present paper, by means of the isolation and chromosomal mapping of the c0t dna fraction and chromatin immunoprecipitation with anti-l ...201425277538
effect of insecticidal fusion proteins containing spider toxins targeting sodium and calcium ion channels on pyrethroid-resistant strains of peach-potato aphid (myzus persicae).the recombinant fusion proteins pl1a/gna and hv1a/gna contain the spider venom peptides δ-amaurobitoxin-pi1a or ω-hexatoxin-hv1a respectively, linked to snowdrop lectin (gna). pl1a targets receptor site 4 of insect voltage-gated sodium channels (nach), while hv1a targets voltage-gated calcium channels. insecticide-resistant strains of peach-potato aphid (myzus persicae) contain mutations in nach. the pyrethroid-resistant kdr (794j) and super-kdr (uko) strains contain mutations at residues l1014 ...201525077959
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