| alicycliphilus denitrificans gen. nov., sp. nov., a cyclohexanol-degrading, nitrate-reducing beta-proteobacterium. | a facultatively denitrifying bacterium, strain k601t, was isolated at 30 degrees c from a municipal sewage plant on cyclohexanol as sole carbon source and nitrate as electron acceptor. under aerobic conditions this strain used acetate, fumarate, lactate, pyruvate, crotonate, indole, glucose, vanillate, 4-hydroxybenzoate, m-cresol, o-cresol and p-cresol. under denitrifying conditions the strain used cyclohexanol, cyclohexanone, 1,3-cyclohexanedione, 2-cyclohexenone, 1,3-cyclohexanediol (cis and t ... | 2003 | 12661531 |
| physiological and phylogenetic characterization of a stable benzene-degrading, chlorate-reducing microbial community. | a stable anoxic enrichment culture was obtained that degraded benzene with chlorate as an electron acceptor. the benzene degradation rate was 1.65 mm benzene per day, which is similar to reported aerobic benzene degradation rates but 20-1650 times higher than reported for anaerobic benzene degradation. denaturing gradient gel electrophoresis of part of the 16s rrna gene, cloning and sequencing showed that the culture had a stable composition after the seventh transfer. five bacterial clones were ... | 2007 | 17386037 |
| characterization of the polyurethanolytic activity of two alicycliphilus sp. strains able to degrade polyurethane and n-methylpyrrolidone. | two bacterial strains (bq1 and bq8) were isolated from decomposed soft foam. these were selected for their capacity to grow in a minimal medium (mm) supplemented with a commercial surface-coating polyurethane (pu) (hydroform) as the carbon source (mm-puh). both bacterial strains were identified as alicycliphilus sp. by comparative 16s rrna gene sequence analysis. growth in mm-puh showed hyperbolic behavior, with bq1 producing higher maximum growth (17.8 +/- 0.6 mg.ml(-1)) than bq8 (14.0 +/- 0.6 ... | 2007 | 17693569 |
| pseudacidovorax intermedius gen. nov., sp. nov., a novel nitrogen-fixing betaproteobacterium isolated from soil. | a gram-negative, short rod-shaped, nitrogen-fixing bacterium (cc-21(t)) was isolated from a soil sample collected from the regional agricultural research station in kaohsiung county (taiwan). using 16s rrna gene sequence analysis, it could be clearly demonstrated that this isolate was novel: it showed <97 % similarity to species of the genera acidovorax, alicycliphilus, giesbergeria, simplicispira and diaphorobacter. the organism used several organic acids, but only a few sugars as substrates. t ... | 2008 | 18218955 |
| isolation and characterization of alicycliphilus denitrificans strain bc, which grows on benzene with chlorate as the electron acceptor. | a bacterium, strain bc, was isolated from a benzene-degrading chlorate-reducing enrichment culture. strain bc degrades benzene in conjunction with chlorate reduction. cells of strain bc are short rods that are 0.6 microm wide and 1 to 2 microm long, are motile, and stain gram negative. strain bc grows on benzene and some other aromatic compounds with oxygen or in the absence of oxygen with chlorate as the electron acceptor. strain bc is a denitrifying bacterium, but it is not able to grow on ben ... | 2008 | 18791031 |
| fluctuation of microbial activities after influent load variations in a full-scale sbr: recovery of the biomass after starvation. | due to variations in the production levels, a full-scale sequencing batch reactor (sbr) for post-treatment of tannery wastewater was exposed to low and high ammonia load periods. in order to study how these changes affected the n-removal capacity, the microbiology of the reactor was studied by a diverse set of techniques including molecular tools, activity tests, and microbial counts in samples taken along 3 years. the recover capacity of the biomass was also studied in a lab-scale reactor opera ... | 2009 | 19629470 |
| structural differentiation of bacterial communities in indole-degrading bioreactors under denitrifying and sulfate-reducing conditions. | the acclimated, anaerobic microbial community is an efficient method for indole-containing wastewater treatment. however, our understanding of the diversity of indole-degrading communities is still limited. we investigated two anaerobic, indole-decomposing microbial communities under both denitrifying and sulfate-reducing conditions. utilizing a near full-length 16s rrna gene clone library, the most dominant bacteria in the denitrifying bioreactor identified was β-proteobacteria. among these, ba ... | 2010 | 20656022 |
| anaerobic oxidation of arsenite linked to chlorate reduction. | microorganisms play a significant role in the speciation and mobility of arsenic in the environment. in this study, the oxidation of arsenite [as(iii)] to arsenate [as(v)] linked to chlorate (clo₃⁻) reduction was shown to be catalyzed by sludge samples, enrichment cultures (ecs), and pure cultures incubated under anaerobic conditions. no activity was observed in treatments lacking inoculum or with heat-killed sludge, or in controls lacking clo₃⁻. the as(iii) oxidation was linked to the complete ... | 2010 | 20729322 |
| purification, characterization, and cloning of a bifunctional molybdoenzyme with hydratase and alcohol dehydrogenase activity. | a bifunctional hydratase/alcohol dehydrogenase was isolated from the cyclohexanol degrading bacterium alicycliphilus denitrificans dsmz 14773. the enzyme catalyzes the addition of water to α,β-unsaturated carbonyl compounds and the subsequent alcohol oxidation. the purified enzyme showed three subunits in sds gel, and the gene sequence revealed that this enzyme belongs to the molybdopterin binding oxidoreductase family containing molybdopterins, fad, and iron-sulfur clusters. | 2010 | 21120472 |
| genome sequences of alicycliphilus denitrificans strains bc and k601t. | alicycliphilus denitrificans strain bc and a. denitrificans strain k601(t) degrade cyclic hydrocarbons. these strains have been isolated from a mixture of wastewater treatment plant material and benzene-polluted soil and from a wastewater treatment plant, respectively, suggesting their role in bioremediation of soil and water. although the strains are phylogenetically closely related, there are some clear physiological differences. the hydrocarbon cyclohexanol, for example, can be degraded by st ... | 2011 | 21742888 |
| nitrate-dependent degradation of acetone by alicycliphilus and paracoccus strains and comparison of acetone carboxylase enzymes. | a novel acetone-degrading, nitrate-reducing bacterium, strain kn bun08, was isolated from an enrichment culture with butanone and nitrate as the sole sources of carbon and energy. the cells were motile short rods, 0.5 to 1 by 1 to 2 μm in size, which gave gram-positive staining results in the exponential growth phase and gram-negative staining results in the stationary-growth phase. based on 16s rrna gene sequence analysis, the isolate was assigned to the genus alicycliphilus. besides butanone a ... | 2011 | 21841031 |