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[a paragonial substance extracted from spermatophores of acanthoscelides obtectus say (coleoptera: bruchidae). isolation of the active fraction].a compound responsible for the stimulation of oogenesis observed after copulation is present in the spermatophores produced by the male accessory glands of the bean weevil acanthoscelides obtectus. a purified biologically active fraction of low molecular weight has been obtained by repeated chromatographies on "sephadex" columns.1975808308
[a paragonial substance extracted from spermatophores of acanthoscelides obtectus say (coleoptera bruchidae). effects on female reproduction].a paragonial substance extracted from the spermatophores is purified by repeated chromatographies on "sephadex" columns. this substance is diluted in physiological serum and injected into the abdomen of virgin females. concentrations of paragonial substance from 0,4 to 0,8 mug/mul stimulate oogenesis. higher concentrations (1,5 to 3 mug/mul) have a toxic effect and cause serious mortality.1975808322
[a male-linked factor determines the arrangement of eggs laid by acanthoscelides obtectus say (coleoptera: bruchidae)]. 1975809173
(-) methyl n-tetradeca-trans-2,4,5-trienoate, an allenic ester produced by the male dried bean beetle, acanthoscelides obtectus (say). 19705461800
evolutionary relationships among proteins in the phytohemagglutinin-arcelin-alpha-amylase inhibitor family of the common bean and its relatives.the common bean, phaseolus vulgaris, contains a family of defense proteins that comprises phytohemagglutinin (pha), arcelin, and alpha-amylase inhibitor (alpha ai). here we report eight new derived amino acid sequences of genes in this family obtained with either the polymerase chain reaction using genomic dna, or by screening cdna libraries made with rna from developing beans. these new sequences are: two alpha ai sequences and arcelin-4 obtained from a wild accession of p. vulgaris that is res ...19947811969
protective mechanism of the mexican bean weevil against high levels of alpha-amylase inhibitor in the common bean.alpha-amylase inhibitor (alpha ai) protects seeds of the common bean (phaseolus vulgaris) against predation by certain species of bruchids such as the cowpea weevil (callosobruchus maculatus) and the azuki bean weevil (callosobruchus chinensis), but not against predation by the bean weevil (acanthoscelides obtectus) or the mexican bean weevil (zabrotes subfasciatus), insects that are common in the americas. we characterized the interaction of alpha ai-1 present in seeds of the common bean, of a ...19968787024
activity of superoxide dismutase and catalase in the bean weevil (acanthoscelides obtectus) selected for postponed senescence.relationship of superoxide dismutase and catalase activities and aging were tested using bean weevil lines selected for postponed senescence. the beetles of different age (young and old) and mating status (virgin and mated) from the extended longevity lines were compared with their counterparts derived from the short-lived lines for activities of sod and catalase. the old beetles from the long-lived lines had statistically significant higher activity of sod than their controls. although we did n ...199910363786
activity of wheat alpha-amylase inhibitors towards bruchid alpha-amylases and structural explanation of observed specificities.plant alpha-amylase inhibitors show great potential as tools to engineer resistance of crop plants against pests. their possible use is, however, complicated by observed variations in specificity of enzyme inhibition, even within closely related families of inhibitors. five alpha-amylase inhibitors of the structural 0.19 family were isolated from wheat kernels, and assayed against three insect alpha-amylases and porcine pancreatic alpha-amylase, revealing several intriguing differences in inhibi ...200010759839
purification, biochemical characterisation and partial primary structure of a new alpha-amylase inhibitor from secale cereale (rye).plant alpha-amylase inhibitors show great potential as tools to engineer resistance of crop plants against pests. their possible use is, however, complicated by the observed variations in specificity of enzyme inhibition, even within closely related families of inhibitors. better understanding of this specificity depends on modelling studies based on ample structural and biochemical information. a new member of the alpha-amylase inhibitor family of cereal endosperm has been purified from rye usi ...200011137459
resource assessment and clutch size in the bean weevil, acanthoscelides obtectus.bean weevil (acanthoscelides obtectus (say)) females were found to use seeds (discrete resource patches) differentially when different sizes were offered in multiple-choice tests. females, either as a group or as individuals, laid significantly (two to six times) more eggs on large seeds than on those of five times smaller mass. in contrast, seed shape (flattened or spherical) did not contribute to clutch-size adjustment. thus, a obtectus females seem to measure only relative seed size when a co ...200312701704
the effect of arcelin-1 on the structure of the midgut of bruchid larvae and immunolocalization of the arcelin protein.some wild accessions of the common bean (phaseolus vulgaris) contain a family of proteins called arcelins, that are toxic to the larvae of certain bruchid species. among the six allelic variants of arcelin tested so far, arcelin-5 and arcelin-1 confer the highest level of resistance against the mexican bean weevil, zabrotes subfasciatus. the same proteins are not toxic to the bean weevil, acanthoscelides obtectus, which is also a serious pest of cultivated beans. arcelins belong to the bean lect ...200012770203
[study of the numerical development of populations of acanthoscelides obtectus say; influence of the initial sexual levels]. 196414289112
use of phage display to select novel cystatins specific for acanthoscelides obtectus cysteine proteinases.cysteine proteinases from larvae of the common bean weevil, acanthoscelides obtectus (coleoptera: bruchidae), were isolated by ion exchange affinity chromatography on a cm-cellulose column and used to select mutant cystatins from a library made with the filamentous m13 phage display system. the library contained variant cystatins derived from the nematode onchocerca volvulus cystatin through mutagenesis of loop 1, which contains the qvvag motif that is involved in binding to proteinases. after t ...200314499599
crystallization and x-ray crystallographic studies of an inhibitor from rye (secale cereale) active against acanthoscelides obtectus and zabrotes subfasciatus alpha-amylases.crystals of a new inhibitor present in rye seeds active against alpha-amylases from crop pests acanthoscelides obtectus and zabrotes subfasciatus have been obtained. a native dataset was collected at 2.21 a resolution with 99.3% completeness at cpr beamline at lnls. the crystals belong to the trigonal system, space group p3(1)21 with a=b=78.21 a, and c=59.61 a. the crystal calculated solvent content is compatible with one dimer per asymmetric unit.200414965283
a kunitz-type inhibitor of coleopteran proteases, isolated from adenanthera pavonina l. seeds and its effect on callosobruchus maculatus.the cowpea weevil callosobruchus maculatus is one of the major pests of vigna unguiculata cowpea. digestion in the cowpea weevil is facilitated by high levels of cysteine and aspartic acid proteinases. plants synthesize a variety of molecules, including proteinaceous proteinase inhibitors, to defend themselves against attack by insects. in this work, a trypsin inhibitor (apti) isolated from adenanthera pavonina seeds showed activity against papain. the inhibition of papain by apti was of the non ...200415113152
the relationship between the chemical composition of three essential oils and their insecticidal activity against acanthoscelides obtectus (say).the chemical composition of the essential oils isolated from various parts of three greek aromatic plants (lavandula hybrida rev, rosmarinus officinalis l and eucalyptus globulus labill) collected at different seasons was determined by gc/ms analysis. the insecticidal action of these oils and of their main constituents on acanthoscelides obtectus (say) adults was evaluated and their lc50 values were estimated. all essential oils tested exhibited strong activity against a. obtectus adults, with v ...200415154521
[toxicity of ddt and hch to calandra granaria and acanthoscelides obtectus]. 194915421606
antimicrobial and insecticidal protein isolated from seeds of clitoria ternatea, a tropical forage legume.the tropical forage legume clitoria ternatea (l.) has important agronomic traits such as adaptation to a wide range of soil conditions and resistance to drought. it is resistant to a number of pathogens and pests. these important traits gave us reasons to look more closely at the plant. a highly basic small protein was purified from seeds of c. ternatea to homogeneity by using ultrafiltration with centricon-3 membrane tubes and preparative granulated-bed isoelectric focusing (ief). a single prot ...200415694280
characterization of two acanthoscelides obtectus alpha-amylases and their inactivation by wheat inhibitors.wheat alpha-amylase inhibitors represent an important tool in engineering crop plants against bean bruchids. because acanthoscelides obtectus is a devastating storage bean insect-pest, we attempted to purify and characterize its gut alpha-amylases, to study their interaction with active proteinaceous inhibitors. two digestives alpha-amylases (aoa1 and aoa2) were purified from gut larvae, showing molecular masses of 30 and 45 kda for each one, respectively. the stoichiometry interaction between t ...200515740044
ancient and recent evolutionary history of the bruchid beetle, acanthoscelides obtectus say, a cosmopolitan pest of beans.acanthoscelides obtectus say is a bruchid species of neotropical origin, and is specialized on beans of the phaseolus vulgaris l. group. since the domestication and diffusion of beans, a. obtectus has become cosmopolitan through human-mediated migrations and is now a major pest in bean granaries. using phylogeographic methods applied to mitochondrial dna (mtdna) and nuclear microsatellite molecular markers, we show that the origin of this species is probably further south than mesoamerica, as co ...200515773933
how host larval age, and nutrition and density of the parasitoid dinarmus basalis (hymenoptera: pteromalidae) influence control of acanthoscelides obtectus (coleoptera: bruchidae).choice of the targeted host developmental stage, regulation of parasitoid numbers released and introduction of food supplements are operational factors with a potential to influence the level of biological control. in a closed laboratory storage system maintained over two generations of the host, the impact of these three parameters on the control potential of the parasitoid dinarmus basalis rondani was investigated for high populations of larvae of acanthoscelides obtectus (say) feeding inside ...200515877863
a new route to methyl (r,e)-(-)-tetradeca-2,4,5-trienoate (pheromone of acanthoscelides obtectus) utilizing a palladium-catalyzed asymmetric allene formation reaction.[reaction: see text] a formal total synthesis of the sex attractant of male dried bean beetle, methyl (r,e)-(-)-tetradeca-2,4,5-trienoate, was achieved by a new efficient route utilizing the pd-catalyzed asymmetric allene synthesis reaction. it was found that the atropisomeric biaryl bisphosphine (r)-segphos showed better enantioselectivity than (r)-binap in the pd-catalyzed reaction for preparing alkyl-substituted axially chiral allenes.200515989370
molecular cloning and expression of an alpha-amylase inhibitor from rye with potential for controlling insect pests.alpha-amylase inhibitors have important roles in plant defense mechanisms, particularly against insects, and several of these inhibitors have been expressed in different crops to increase their resistance to particular insects. in this work, we report the cloning and expression of a gene encoding for a new alpha-amylase inhibitor (biii) from rye (secale cereale) seeds. the biii gene contains 354 nucleotides that encode for 118 amino acids sequence. a 313 bp fragment of the gene was expressed in ...200516003953
molecular modeling and inhibitory activity of cowpea cystatin against bean bruchid pests.plant cystatins show great potential as tools to genetically engineer resistance of crop plants against pests. two important potential targets are the bean weevils acanthoscelides obtectus and zabrotes subfasciatus, which display major activities of digestive cysteine proteinases in midguts. in this study a cowpea cystatin, a cysteine proteinase inhibitor found in cowpea (vigna unguiculata) seeds, was expressed in escherichia coli and purified with a ni-nta agarose column. it strongly inhibited ...200616470583
isolation and partial characterization of a seed lectin from tepary bean that delays bruchid beetle development.four isolectin forms of a seed lectin from mature seed of tepary bean (phaseolus acutifolius) were isolated using solubility fractionation, affinity chromatography, and high performance liquid chromatography. the subunits are polypeptides with an apparent molecular mass of 30,000 daltons. the 30 kilodalton subunits are produced starting approximately 13 days after flowering and subsequently comprise a major fraction of the proteins found in the mature seed. the amino terminus of each isolectin f ...199016667639
phylogeographic support for horizontal gene transfer involving sympatric bruchid species.we report on the probable horizontal transfer of a mitochondrial gene, cytb, between species of neotropical bruchid beetles, in a zone where these species are sympatric. the bruchid beetles acanthoscelides obtectus, a. obvelatus, a. argillaceus and zabrotes subfasciatus develop on various bean species in mexico. whereas a. obtectus and a. obvelatus develop on phaseolus vulgaris in the mexican altiplano, a. argillaceus feeds on p. lunatus in the pacific coast. the generalist z. subfasciatus feeds ...200616872524
biochemistry of insect epicuticle degradation by entomopathogenic fungi.the biochemical interaction between fungal pathogens and their insect host epicuticle was studied by examining fungal hydrocarbon degrading ability. as a contact insecticide, entomopathogenic fungi invade their host through the cuticle, covered by an outermost lipid layer mainly composed of highly stable, very long chain structures. strains of beauveria bassiana and metarhizium anisopliae (deuteromycotina: hyphomycetes), pathogenic both to the blood-sucking bug triatoma infestans (hemiptera: red ...200717052960
ageing and the evolution of female resistance to remating in seed beetles.female remating behaviour is a key mating system parameter that is predicted to evolve according to the net effect of remating on female fitness. in many taxa, females commonly resist male remating attempts because of the costs of mating. here, we use replicated populations of the seed beetle acanthoscelides obtectus selected for either early or late life reproduction and show that 'early' and 'late' females evolved different age-specific rates of remating. early females were more likely to rema ...200617148327
genetic architecture of differences in oviposition preference between ancestral and derived populations of the seed beetle acanthoscelides obtectus.we investigated the additive, dominance and epistatic genetic effects underlying differentiation in oviposition preference between two populations of the seed beetle acanthoscelides obtectus evolved in the laboratory for 102 generations on bean and chickpea seeds. we reared and tested females on each of two host legumes. the populations differed in mean oviposition preference; the preference for chickpea was stronger in population reared on the chickpea (c) than in population maintained on commo ...200717301741
proregion of acanthoscelides obtectus cysteine proteinase: a novel peptide with enhanced selectivity toward endogenous enzymes.acanthoscelides obtectus is a devastating storage insect pest capable of causing severe bean crop losses. in order to maintain their own development, insect pest larvae feed continuously, synthesizing efficient digestive enzymes. among them, cysteine proteinases (cps) are commonly produced as inactive precursors (procysteines), requiring a cleavage of the peptide proregion to become active. the proregion fits tightly into the active site of procysteines, efficiently preventing their activity. in ...200717485144
a recombinant form of chagasin from trypanosoma cruzi: inhibitory activity on insect cysteine proteinases.the activity of the major digestive cysteine proteinase detected in the intestinal tract of larvae of the bean weevil, acanthoscelides obtectus (say), was efficiently inhibited by the well-characterized cysteine proteinase synthetic inhibitor e-64 and also by a recombinant form of chagasin (r-chagasin), a tight-binding cysteine proteinase inhibitor protein from trypanosoma cruzi.200818318460
optimal xylocoris flavipes (reuter) (hemiptera: anthocoridae) density and time of introduction for suppression of bruchid progeny in stored legumes.the influences of both predator density and elapsed time between initial infestation and introduction of predators were determined for suppression of bruchids infesting stored grain legumes by xylocoris flavipes (reuter) (hemiptera: anthocoridae). predator density treatments consisted of zero, one, two, three, or five male:female pairs of adult x. flavipes added to experimental arenas 0, 24, or 120 h after bruchid introduction. suppression of acanthoscelides obtectus approached eradication with ...200818348804
novel insights on the mechanism of action of alpha-amylase inhibitors from the plant defensin family.plant defensins are small cysteine-rich proteins commonly synthesized in plants, encoded by large multigene families. most plant defensins that have been characterized to date show potent antifungal and/or bactericidal activities. this report describes vud1, an unusual defensin that is able to inhibit insect-pest alpha-amylases. vud1 was cloned from cowpea (vigna unguiculata) seeds and expressed in a heterologous system. inhibitory enzyme assays showed that vud1 efficiently inhibits alpha-amylas ...200818498107
effects of different temperatures on the total carbohydrate, lipid and protein amounts of the bean beetle, acanthoscelides obtectus say (coleoptera: bruchidae).this study investigates the effects of different temperatures on the total carbohydrate, lipid and protein amounts of acanthoscelides obtectus say, which is a common cereal pest. studies have been carried out under laboratory conditions at 20 +/- 2 degrees c, 30 +/- 2 degrees c and 60 +/- 5% relative humidity. no specific photoperiodic regimen has been used throughout the study. total carbohydrate, protein and lipid amounts for females at 20 degrees c were 61.74, 35.77 and 83.79 microg/individua ...200818817220
characterization of a hydrophobic amylase inhibitor from corn (zea mays) seeds with activity against amylase from fusarium verticillioides.abstract a hydrophobic 19.7-kda amylase inhibitor (ai) was purified from corn kernels by 95% ethanol extraction and anionic exchange chromatography. the ai has an isoelectric point of 3.6 and was very stable at different ph values and high temperatures, maintaining 47.6% activity after heating to 94 degrees c for 60 min. amino acid analysis indicated high valine, leucine, glycine, alanine, and glutamic acid/glutamine content, and especially high valine content (41.2 mol%). this inhibitor is not ...200318943857
insecticidal activity of the essential oils from different plants against three stored-product insects.this study was conducted to determine the insecticidal activity of essential oils from oregano, origanum onites l. (lamiales: lamiaceae), savory, satureja thymbra l. (lamiales: lamiaceae), and myrtle, myrtus communis l. (rosales: myrtaceae) against three stored-product insects. essential oils from three species of plants were obtained by clevenger-type water distillation. the major compounds in these essential oils were identified using gas chromatography-mass spectrometry and their insecticidal ...201020578885
inhibitory activity of beauveria bassiana and trichoderma spp. on the insect pests xylotrechus arvicola (coleoptera: cerambycidae) and acanthoscelides obtectus (coleoptera: chrisomelidae: bruchinae).xylotrechus arvicola is an important pest in vineyards (vitis vinifera) in the main iberian wine-producing regions, and acanthoscelides obtectus causes severe post-harvest losses in the common bean (phaseolus vulgaris). under laboratory conditions with a spray tower, the susceptibility of the immature stages of x. arvicola and a. obtectus against the entomopathogenic fungi beauveria bassiana and four strains of trichoderma spp. was evaluated. both insect pests t. harzianum and b. bassiana showed ...201727933578
antimicrobial, insecticidal and phytotoxic activities of cotinus coggyria scop. essential oil (anacardiaceae).the essential oil of cotinus coggyria scop.' leaves was found to be rich in α-pinene (43.1%), limonene (21.3%) and β-myrcene (8.5%). in the antimicrobial screening, essential oil was notably active on staphylococcus aureus atcc 29213, s. aureus atcc baa-977, candida albicans atcc 14053 and c. parapsilosis atcc 22019 using the disc diffusion and volatilisation assays. the fumigant assay of the essential oil caused 70% and 100% mortality on the two pest adults of acanthoscelides obtectus and tribo ...201424980636
multifunctional amaranth cystatin inhibits endogenous and digestive insect cysteine endopeptidases: a potential tool to prevent proteolysis and for the control of insect pests.in a previous study, the amaranth cystatin was characterized. this cystatin is believed to provide protection from abiotic stress because its transcription is induced in response to heat, drought, and salinity. it has also been shown that recombinant amaranth cystatin inhibits bromelain, ficin, and cysteine endopeptidases from fungal sources and also inhibits the growth of phytopathogenic fungi. in the present study, evidence is presented regarding the potential function of amaranth cystatin as ...201525345487
resistance of αai-1 transgenic chickpea (cicer arietinum) and cowpea (vigna unguiculata) dry grains to bruchid beetles (coleoptera: chrysomelidae).dry grain legume seeds possessing αai-1, an α-amylase inhibitor from common bean (phaseolus vulgaris), under the control of a cotyledon-specific promoter have been shown to be highly resistant to several important bruchid pest species. one transgenic chickpea and four cowpea lines expressing αai-1, their respective controls, as well as nine conventional chickpea cultivars were assessed for their resistance to the bruchids acanthoscelides obtectus (say), callosobruchus chinensis l. and callosobru ...201323458831
activity of eight strains of entomopathogenic nematodes (rhabditida: steinernematidae, heterorhabditidae) against five stored product pests.stored product pests are responsible for losses that can amount 10% during cereal storage in the world. aiming to find an alternative method to the chemicals used for the stored-product pests, eight strains of entomopathogenic nematodes (epns) were tested against five species of stored product pests. the bioassays were conducted in microtubes containing paper, inoculated with epns and insect diet. all the insect species were susceptible to the epns strains. anagasta kuehniella and tenebrio molit ...201323567251
antennal and mouthpart sensilla of acanthoscelides obtectus say (coleoptera: bruchidae).acanthoscelides obtectus, a serious pest with a worldwide distribution, damages stored seeds of phaseolus sp. to acquire a better understanding of the sensory orientation of bean weevils, their antennal and palpal sensilla were investigated. the antennae and palps possess several types of sensory hairs: blunt-tipped sensilla chaetica and trichoidea and also sharp-tipped sensilla trichoidea, these last being the most numerous. sensilla basiconica are present only on the flagellomeres, but böhm br ...201627696563
no correlation of morpho-agronomic traits of phaseolus vulgaris (fabaceae) genotypes and resistance to acanthoscelides obtectus (say) and zabrotes subfasciatus (boheman) (coleoptera: chrysomelidae).resistance of common bean (phaseolus vulgaris) varieties is an important tool to control acanthoscelides obtectus (say) and zabrotes subfasciatus (boheman) (coleoptera: chrysomelidae: bruchinae) worldwide. however, bioassays to characterize the resistance of a genotype can be difficult to perform. therefore, the current study sought to correlate the morpho-agronomic traits of p. vulgaris genotypes with their resistance to a. obtectus and z. subfasciatus to facilitate genotype characterization. b ...201526253545
experimentally induced host-shift changes life-history strategy in a seed beetle.expansion of the host range in phytophagous insects depends on their ability to form an association with a novel plant through changes in host-related traits. phenotypic plasticity has important effects on initial survival of individuals faced with a new plant, as well as on the courses of evolutionary change during long-term adaptation to novel conditions. using experimental populations of the seed beetle that evolved on ancestral (common bean) or novel (chickpea) host and applying reciprocal t ...201626790127
triple-layer plastic bags protect dry common beans (phaseolus vulgaris) against damage by acanthoscelides obtectus (coleoptera: chrysomelidae) during storage.fumigated dry common beans (phaseolus vulgaris l.) that were artificially infested with acanthoscelides obtectus say, and others that were not artificially infested, were stored in hermetic triple-layer pics (lela agro, kano, nigeria) or woven polypropylene (pp) bags for 6 mo at ambient laboratory temperature conditions of 22.6 ± 1.9°c and 60.1 ± 4.3% relative humidity. in an additional trial, beans contained in pp bags were treated with actellic super dust before introducing a. obtectus. moistu ...201526453738
assessment of insecticidal efficacy of diatomaceous earth and powders of common lavender and field horsetail against bean weevil adults.in the search for an effective and sustainable control method against the bean weevil acanthoscelides obtectus (say), an important insect pest affecting stored common beans and other legumes, three different powders were tested against adult been weevils under laboratory conditions. the three powders were diatomaceous earth (de) (commercial product silicosec®), common lavender (lavandula angustifolia) powder and field horsetail (equisetum arvense) powder. the substances were tested at five tempe ...201327193283
mesoamerican origin and pre- and post-columbian expansions of the ranges of acanthoscelides obtectus say, a cosmopolitan insect pest of the common bean.an unprecedented global transfer of agricultural resources followed the discovery of the new world; one consequence of this process was that staple food plants of neotropical origin, such as the common bean (phaseolus vulgaris), soon expanded their ranges overseas. yet many pests and diseases were also transported. acanthoscelides obtectus is a cosmopolitan seed predator associated with p. vulgaris. codispersal within the host seed seems to be an important determinant of the ability of a. obtect ...201323936139
bottom-up and top-down effects influence bruchid beetle individual performance but not population densities in the field.plant quality (bottom-up) and natural enemies (top-down) can influence the individual performance of herbivorous insects on their host plants, but few studies measured at the same time the influence on population densities in the field. we investigated if plant quality of different wild common bean populations, phaseolus vulgaris l. (fabaceae), affects the performance of the bean weevil, acanthoscelides obtectus (say) (coleoptera: bruchidae), and one of its enemies, the ectoparasitoid dinarmus b ...201323383151
ques, a new phaseolus vulgaris genotype resistant to common bean weevils, contains the arcelin-8 allele coding for new lectin-related variants.in common bean (phaseolus vulgaris l.), the most abundant seed proteins are the storage protein phaseolin and the family of closely related apa proteins (arcelin, phytohemagglutinin and α-amylase inhibitor). high variation in apa protein composition has been described and the presence of arcelin (arc) has been associated with bean resistance against two bruchid beetles, the bean weevil (acanthoscelides obtectus say) and the mexican bean weevil (zabrotes subfasciatus bohemian). so far, seven arc ...201323117719
bioefficacy of essential and vegetable oils of zanthoxylum xanthoxyloides seeds against acanthoscelides obtectus (say) (coleoptera: bruchidae).experiments were conducted in the laboratory to evaluate the bioefficacy of essential and vegetable oils of zanthoxylum xanthoxyloides (rutaceae) against acanthoscelides obtectus (coleoptera: bruchidae). the chemical composition of the essential oil and the fatty acid composition of the vegetable oil extracted from the seeds of z. xanthoxyloides were determined. the insecticidal activities of these oils and the associated aromatized clay powder were evaluated against a. obtectus. both oils were ...201222410230
comparison of four populations of acanthoscelides obtectus (coleoptera: bruchidae) from different colombian ecosystems : assay of interpretation.the bean weevil (acanthoscelides obtectus) and its preferential host plant phaseolus vulgaris probably originate from central or south america. acanthoscelides obtectus populations have developed for many years in varied and relatively isolated ecosystems in these regions. the influence of the host and of copulation on reproductive activity was studied in four colombian insect populations from medium (2000 m) and low altitudes (1200 m). most medium altitude strains of females do not produce oocy ...197828310276
evaluation of the natural zeolite lethal effects on adults of the bean weevil under different temperatures and relative humidity regimes.we studied the insecticidal activity of different concentrations of very high quality natural zeolites (zeolitic rock containing 92 wt% clinoptilolite) applied on dry beans. the test species was adult bean weevils acanthoscelides obtectus (say; coleoptera: bruchidae), and the variables included different temperatures and humidity regimes. at certain natural zeolite concentrations the adult mortality approached 100% within the first day of exposure. the lethal natural zeolite concentration for 50 ...201729240918
[the dorsoventral inversion: an attempt of synthesis].the invertebrates, with known exception of echinoderms, are hyponeurian and protostomian. by contrast, echinoderms, chordates and vertebrate are epineurian and deuterostomian. convinced of the uniqueness origin of all species, etienne geoffroy saint hilaire (1772-1844), had postulated a complete inversion of body plan to explain this difference. he had to face up to the hostility of the fixist georges cuvier (1763-1832). much later, famous embryologists such as maurice caullery still believed th ...201728964656
characterization of the complete mitochondrial genome of acanthoscelides obtectus (coleoptera: chrysomelidae: bruchinae) with phylogenetic analysis.acanthoscelides obtectus is a common species of the subfamily bruchinae and a worldwide-distributed seed-feeding beetle. the complete mitochondrial genome of a. obtectus is 16,130 bp in length with an a + t content of 76.4%. it contains a positive at skew and a negative gc skew. the mitogenome of a. obtectus contains 13 protein-coding genes (pcgs), 22 trna genes, two rrna genes and a non-coding region (d-loop). all pcgs start with an atn codon, and seven (nd3, atp6, coiii, nd3, nd4l, nd6, and cy ...201728730527
insecticidal effects of extracts of humulus lupulus (hops) l. cones and its principal component, xanthohumol.insecticidal effects of the dichloromethane, ethyl acetate, acetone, ethanol and methanol extracts of humulus lupulus (hops) l. cones and its principal components, xanthohumol was investigated on five stored pests, sitophilus granarius (l.), sitophilus oryzae (l.), acanthoscelides obtectus (say.), tribolium castaneum (herbst) and lasioderma serricorne (f.). the mortality of adults of the insects treated with 2, 5, 5, 10 and 20 mg ml̠-1 concentrations of the extracts and xanthuhumol was counted a ...201728330519
sex-specific mitonuclear epistasis and the evolution of mitochondrial bioenergetics, ageing, and life history in seed beetles.the role of mitochondrial dna for the evolution of life-history traits remains debated. we examined mitonuclear effects on the activity of the multisubunit complex of the electron transport chain (etc) involved in oxidative phosphorylation (oxphos) across lines of the seed beetle acanthoscelides obtectus selected for a short (e) or a long (l) life for more than >160 generations. we constructed and phenotyped mitonuclear introgression lines, which allowed us to assess the independent effects of t ...201727861795
divergent evolution of life span associated with mitochondrial dna evolution.mitochondria play a key role in ageing. the pursuit of genes that regulate variation in life span and ageing have shown that several nuclear-encoded mitochondrial genes are important. however, the role of mitochondrial encoded genes (mtdna) is more controversial and our appreciation of the role of mtdna for the evolution of life span is limited. we use replicated lines of seed beetles that have been artificially selected for long or short life for >190 generations, now showing dramatic phenotypi ...201727778315
multiple roles of a male-specific compound in the sexual behavior of the dried bean beetle, acanthoscelides obtectus.males of acanthoscelides obtectus (coleoptera: chrysomelidae, bruchinae) emit methyl (e,r)-2,4,5-tetradecatrienoate that attracts females for mating. this study identified further roles for this compound in the sexual behavior of a. obtectus. earlier observations revealed that males touched females with their antennae while tandem-running with them and initiated mounting and copulation, whereas they showed no such behavior toward other males. a series of subsequent laboratory choice tests were s ...201525786892
the effect of mitochondrial complex i inhibitor on longevity of short-lived and long-lived seed beetles and its mitonuclear hybrids.mitochondria are suggested to play a central role in ageing and evolution of longevity. gradual decline in mitochondrial function during ageing and concomitant increase in production of reactive oxygen species (ros) leads to oxidative damage of macromolecules and impairment of atp synthesis. to assess relationship between ageing and oxidative stress resistance we exposed different longevity lines of the seed beetle (acanthoscelides obtectus) to four concentrations of tebufenpyrad, mitochondrial ...201425078074
heterosis in age-specific selected populations of a seed beetle: sex differences in longevity and reproductive behavior.we tested mutation accumulation hypothesis for the evolution of senescence using short-lived and long-lived populations of the seed-feeding beetle, acanthoscelides obtectus (say), obtained by selection on early- and late-life for many generations. the expected consequence of the mutation accumulation hypothesis is that in short-lived populations, where the force of natural selection is the strongest early in life, the late-life fitness traits should decline due to genetic drift which increases t ...201524677595
resistance to prooxidant agent paraquat in the short- and long-lived lines of the seed beetle (acanthoscelides obtectus).in the present study we test whether variation in resistance to paraquat (pq), a free radical generator, correlates with variation in longevity in two sets of seed beetles (acanthoscelides obtectus) experimental lines that were selected either for early reproduction and short-life or late reproduction and long-life. long-lived late reproduction lines (l) showed increased resistance to pq, while opposite was true for short-lived early reproduction line (e). striking outcome of the selection for e ...201323515831
laboratory evolution of life-history traits in the bean weevil (acanthoscelides obtectus): the effects of selection on developmental time in populations with different previous history.in this study we examined the direct and correlated responses for fast and slow preadult development time in three laboratory populations of the bean weevil (acanthoscelides obtectus). the first population ("base," b) has experienced laboratory conditions for more than 10 years; the second ("young," y) and the third ("old," o) populations were selected for early and late reproduction, respectively, before the onset of the present experiments. all three populations are successfully selected for b ...199828565311
laboratory evolution of life-history traits in the bean weevil (acanthoscelides obtectus): the effects of density-dependent and age-specific selection.four types of laboratory populations of the bean weevil (acanthoscelides obtectus) have been developed to study the effects of density-dependent and age-specific selection. these populations have been selected at high (k) and low larval densities (r) as well as for reproduction early (y) and late (o) in life. the results presented here suggest that the r- and k-populations (density-dependent selection regimes) have differentiated from each other with respect to the following life-history traits: ...199728565104
preference for magnesium sulfate-treated leguminous seeds in egg-laying bean weevil (acanthoscelides obtectus say, col., bruchidae).unlike many secondary plant substances, a wide range of concentrations (4-1000 mm) of magnesium sulfate, applied to dry beans, significantly increased egg-laying by the dry bean weevil in binary choice tests, in favor of treated seeds. no other magnesium-containing compounds studied exerted such an effect, nor was a similar response noted on treated beans in no-choice situations. the total number of eggs laid per female was in the same range in both types of test. variably enhanced or suppressed ...198924272097
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