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endophytic bacterial communities in ginseng and their antifungal activity against pathogens.plant roots are associated with diverse communities of endophytic bacteria which do not exert adverse effects. the diversity of bacterial endophytes associated with ginseng roots cultivated in three different areas in korea was investigated. sixty-three colonies were isolated from the interior of ginseng roots. phylogenetic analysis based on 16s rrna gene sequences showed that the isolates belonged to three major phylogenetic groups: the high g+c gram-positive bacteria (hgcgpb), low g+c gram-pos ...200717492474
genetic diversity of siderophore-producing bacteria of tobacco rhizosphere.the genetic diversity of siderophore-producing bacteria of tobacco rhizosphere was studied by amplified ribosomal dna restriction analysis (ardra), 16s rrna sequence homology and phylogenetics analysis methods. studies demonstrated that 85% of the total 354 isolates produced siderophores in iron limited liquid medium. a total of 28 ardra patterns were identified among the 299 siderophore-producing bacterial isolates. the 28 ardra patterns represented bacteria of 14 different genera belonging to ...200924031358
isolation and characterization of onion degrading bacteria from onion waste produced in south buenos aires province, argentina.onion production in argentina generates a significant amount of waste. finding an effective method to recycle it is a matter of environmental concern. among organic waste reuse techniques, anaerobic digestion could be a valuable alternative to current practices. substrate inoculation with appropriate bacterial strains enhances the rate-limiting step (hydrolysis) of anaerobic digestion of biomass wastes. selection of indigenous bacteria with the ability to degrade onion waste could be a good appr ...201525586510
characterization of aerobic oil and grease-degrading bacteria in wastewater.a bacterial consortium that degrades cooking oil (co) has been isolated in wastewater (ww) samples, by enrichment in olive co. this consortium could degrade 90% of co within 7-9 days (from an initial 1% [w/v]), and it is more active at alkaline conditions. the 16s ribonucleic acid (rna) gene analysis showed that it contains five bacterium species: stenotrophomonas rhizophila, sphingobacterium sp., pseudomonas libanensis, pseudomonas poae and pseudomonas aeruginosa. this consortium can degrade th ...201727426954
methods for the comprehensive structural elucidation of constitution and stereochemistry of lipopeptides.a panel of methods of general suitability for complete structural elucidation of the stereochemistry of cyclopeptides, depsipeptides and lipopeptides is presented and described in detail. the suitability of the proposed methods was exemplified on the lipopeptide poaeamide from pseudomonas poae. amino acid configurations have been assigned by direct lc enantiomer separation with chiralpak zwix(+) and were confirmed by gc enantiomer separation on chirasil l-val. 3-hydroxydecanoic acid absolute con ...201626087965
the novel lipopeptide poaeamide of the endophyte pseudomonas poae re*1-1-14 is involved in pathogen suppression and root colonization.endophytic pseudomonas poae strain re*1-1-14 was originally isolated from internal root tissue of sugar beet plants and shown to suppress growth of the fungal pathogen rhizoctonia solani both in vitro and in the field. to identify genes involved in its biocontrol activity, re*1-1-14 random mutagenesis and sequencing led to the identification of a nonribosomal peptide synthetase (nrps) gene cluster predicted to encode a lipopeptide (lp) with a 10-amino-acid peptide moiety. the two unlinked gene c ...201525761208
complete genome sequence of the sugar beet endophyte pseudomonas poae re*1-1-14, a disease-suppressive bacterium.the endophytic bacterium pseudomonas poae re*1-1-14 shows broad antagonistic activity and is applied to seeds as a biocontrol agent to suppress late root rot in the sugar beet. the completely sequenced 5.5-mb genome reveals genes that putatively contribute to this antagonistic activity and the intimate plant-microbe interaction.201323516179
effects of illuminance and nutrients on bacterial photo-physiology of hydrocarbon degradation.bacterial photophysiology was previously limited to photoautotrophs. the discovery of bacteriophytochromes in non-photoautotrophs raised a question whether these non-photoautotrophs are affected by the presence or absence of light? in this research work for the first time, bacterial hydrocarbon degradation and biomass production was studied under the influence of nutrients, illuminance (light flux) and time. an experimental model was designed, with six isolated bacterial strains (pseudomonas poa ...201627039061
enhanced biological straw saccharification through coculturing of lignocellulose-degrading microorganisms.lignocellulosic waste (lcw) is an abundant, low-cost, and inedible substrate for the induction of lignocellulolytic enzymes for cellulosic bioethanol production using an efficient, environmentally friendly, and economical biological approach. in this study, 30 different lignocellulose-degrading bacterial and 18 fungal isolates were quantitatively screened individually for the saccharification of four different ball-milled straw substrates: wheat, rice, sugarcane, and pea straw. rice and sugarcan ...201525724976
universal and species-specific bacterial 'fungiphiles' in the mycospheres of different basidiomycetous fungi.in previous work, several bacterial groups that show a response to fruiting bodies (the mycosphere) of the ectomycorrhizal fungus laccaria proxima were identified. we here extend this work to a broader range of fungal fruiting bodies sampled at two occasions. pcr-dgge analyses showed clear effects of the mycosphere of diverse fungi on the total bacterial and pseudomonas communities in comparison with those in the corresponding bulk soil. the diversities of the pseudomonas communities increased d ...200919196267
fluorescent pseudomonads associated with the phyllosphere of grasses; pseudomonas trivialis sp. nov., pseudomonas poae sp. nov. and pseudomonas congelans sp. nov.strains of fluorescent pseudomonads, isolated from the phyllosphere of grasses, were analysed by a polyphasic approach in order to clarify their interspecific position. classification on the basis of ribotyping revealed six genotypes; four of these, which could be differentiated clearly from each other and from pseudomonas species with validly published names on the basis of phenotypic features, were chosen for detailed phylogenetic analysis. dna-dna hybridization studies among representative st ...200313130034
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