| apoptotic cell death is a common response to pathogen attack in oats. | we have examined the characteristics of cell death induced by pathogen infection in oats with respect to following hallmark apoptotic features: dna laddering, chromatin condensation, and electron microscopic-terminal deoxynucleotidyl transferase-mediated utp end labeling positive response. a wide range of plant pathogens representing different levels of parasitism in susceptible and resistant interactions were used for the inocula, which include (i) an obligate parasite, puccinia coronata f. sp. ... | 2002 | 12437297 |
| rds and rih mediate hypersensitive cell death independent of gene-for-gene resistance to the oat crown rust pathogen puccinia coronata f. sp. avenae. | the pca crown rust resistance cluster in the diploid avena genus confers gene-for-gene specificity to numerous isolates of puccinia coronata f. sp. avenae. recombination breakpoint analysis indicates that specificities conferred by the pca cluster are controlled by at least five distinct genes, designated pc81, pc82, pc83, pc84, and pc85. avena plants with the appropriate genotype frequently respond to p. coronata by undergoing hypersensitive cell death at the sites of fungal infection. autofluo ... | 2001 | 11768532 |
| development and characterization of novel, polymorphic microsatellite markers for oat crown rust, puccinia coronata. | we report the development of 37 novel and polymorphic microsatellite markers for oat crown rust, puccinia coronata f.sp. avenae. the allelic diversity ranged from two to 16 alleles per locus. observed heterozygosity ranged from 0.000 to 0.971, and expected heterozygosity from 0.057 to 0.848. thirteen of the loci were not in hardy-weinberg equilibrium, due to either the presence of null alleles, small sample size, or the effects of population subdivision (wahlund's effect). all 37 primer pairs we ... | 2008 | 21586056 |
| characterization and mapping of oat crown rust resistance genes using three assessment methods. | abstract resistance is the primary means of control for crown rust of oat (avena sativa l.), caused by puccinia coronata f. sp. avenae, and better knowledge of the genetics of resistance will enhance resistance breeding. disease data were generated in the field and greenhouse for parents and recombinant inbred lines of the ogle/tam o-301 (ot) oat mapping population using (i) a new quantitative assay that employs quantitative real-time polymerase chain reaction (q-pcr) to estimate fungal growth i ... | 2007 | 18944171 |
| quantitative trait loci for partial resistance to crown rust, puccinia coronata, in cultivated oat, avena sativa l. | to facilitate the detection of quantitative trait loci (qtls) for partial resistance to oat crown rust, puccinia coronata f. sp. avenae eriks., a genetic map was generated in a population of 158 f(6)-derived oat recombinant inbred lines from a cross of a partial resistance line mn841801-1 by a susceptible cultivar selection 'noble-2'. the map, developed using 230 marker loci, mostly restriction fragment length polymorphism and amplified fragment length polymorphism markers, spanned 1,509 cm (hal ... | 2005 | 15918009 |
| nitric oxide and reactive oxygen species do not elicit hypersensitive cell death but induce apoptosis in the adjacent cells during the defense response of oat. | nitric oxide (no) acts as a signaling molecule in many cellular responses in plants and animals. oat plants (avena sativa l.) evoke the hypersensitive response (hr), which shares morphological and biochemical features with mammalian apoptosis, such as dna laddering and heterochromatin condensation, in response to the avirulent crown rust fungus (puccinia coronata f. sp. avenae). we examined the role of no and reactive oxygen species (ros) in the initiation of hypersensitive cell death, which is ... | 2004 | 15000391 |