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characterization of endospore-forming bacteria associated with entomopathogenic nematodes, heterorhabditis spp., and description of paenibacillus nematophilus sp. nov.endospore-forming bacteria were isolated from insect-pathogenic nematodes, heterorhabditis spp., from three diverse geographical locations. spindle-shaped sporangia of these bacteria adhere to the free-living infective stage of the nematode, which carries them to new insect hosts, where the bacterium reproduces. these isolates were characterized based on phenotypic and chemotaxonomic properties and 16s rrna gene sequences. analysis of the 16s rrna gene placed the isolates within the genus paenib ...200312710609
effects of paenibacillus nematophilus on the entomopathogenic nematode heterorhabditis megidis.the insect parasitic nematodes heterorhabditis spp. are mutualistically associated with entomopathogenic bacteria, photorhabdus spp. a novel association has been detected between h. megidis isolate eu17 and the endospore-forming bacterium paenibacillus nematophilus. p. nematophilus sporangia adhere to infective juveniles (ijs) of h. megidis and develop in insect hosts along with the nematodes and their symbiont. we tested the effects of p. nematophilus on h. megidis. the yield and quality (size, ...200415707868
entomopathogenic nematodes, phoretic paenibacillus spp., and the use of real time quantitative pcr to explore soil food webs in florida citrus groves.quantitative real-time pcr (qpcr) is a powerful tool to detect and quantify species of cryptic organisms such as bacteria, fungi and nematodes from soil samples. as such, qpcr offers new opportunities to study the ecology of soil habitats by providing a single method to characterize communities of diverse organisms from a sample of dna. here we describe molecular tools to detect and quantify two bacteria (paenibacillus nematophilus and paenibacillus sp.) phoretically associated with entomopathog ...201121723288
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