| characterization of white bream virus reveals a novel genetic cluster of nidoviruses. | the order nidovirales comprises viruses from the families coronaviridae (genera coronavirus and torovirus), roniviridae (genus okavirus), and arteriviridae (genus arterivirus). in this study, we characterized white bream virus (wbv), a bacilliform plus-strand rna virus isolated from fish. analysis of the nucleotide sequence, organization, and expression of the 26.6-kb genome provided conclusive evidence for a phylogenetic relationship between wbv and nidoviruses. the polycistronic genome of wbv ... | 2006 | 16987966 |
| RNA-binding domain in the nucleocapsid protein of gill-associated nidovirus of penaeid shrimp. | Gill-associated virus (GAV) infects Penaeus monodon shrimp and is the type species okavirus in the Roniviridae, the only invertebrate nidoviruses known currently. Electrophoretic mobility shift assays (EMSAs) using His(6)-tagged full-length and truncated proteins were employed to examine the nucleic acid binding properties of the GAV nucleocapsid (N) protein in vitro. The EMSAs showed full-length N protein to bind to all synthetic single-stranded (ss)RNAs tested independent of their sequence. Th ... | 2011 | 21857914 |
| glycosylation of gp116 and gp64 envelope proteins of yellow head virus of penaeus monodon shrimp. | yellow head virus (yhv) is a highly virulent pathogen of penaeus monodon shrimp that is classified in the genus okavirus, family roniviridae, in the order nidovirales. separation of virion proteins treated with peptide-n-glycosidase-f (pngase-f) in sds-polyacrylamide gels and the use of glycoprotein-specific staining methods indicated that the gp116 and gp64 envelope glycoproteins possess n-linked rather than o-linked glycans. competitive binding inhibition of lectins with various oligosaccharid ... | 2010 | 20554800 |
| evidence for a novel coding sequence overlapping the 5'-terminal approximately 90 codons of the gill-associated and yellow head okavirus envelope glycoprotein gene. | the genus okavirus (order nidovirales) includes a number of viruses that infect crustaceans, causing major losses in the shrimp industry. these viruses have a linear positive-sense ssrna genome of approximately 26-27 kb, encoding a large replicase polyprotein that is expressed from the genomic rna, and several additional proteins that are expressed from a nested set of 3'-coterminal subgenomic rnas. in this brief report, we describe the bioinformatic discovery of a new, apparently coding, orf th ... | 2009 | 20017924 |
| the 3c-like proteinase of an invertebrate nidovirus links coronavirus and potyvirus homologs. | gill-associated virus (gav), a positive-stranded rna virus of prawns, is the prototype of newly recognized taxa (genus okavirus, family roniviridae) within the order nidovirales: in this study, a putative gav cysteine proteinase (3c-like proteinase [3cl(pro)]), which is predicted to be the key enzyme involved in processing of the gav replicase polyprotein precursors, pp1a and pp1ab, was characterized. comparative sequence analysis indicated that, like its coronavirus homologs, 3cl(pro) has a thr ... | 2003 | 12502857 |