| methylocella tundrae sp. nov., a novel methanotrophic bacterium from acidic tundra peatlands. | a novel species, methylocella tundrae, is proposed for three methanotrophic strains (t4t, tch1 and ty1) isolated from acidic sphagnum tundra peatlands. these strains are aerobic, gram-negative, non-motile, dinitrogen-fixing rods that possess a soluble methane monooxygenase and utilize the serine pathway for carbon assimilation. strains t4t, tch1 and ty1 are moderately acidophilic organisms capable of growth between ph 4.2 and 7.5 (optimum 5.5-6.0) and between 5 and 30 degrees c (optimum 15 degre ... | 2004 | 14742473 |
| methylocella species are facultatively methanotrophic. | all aerobic methanotrophic bacteria described to date are unable to grow on substrates containing carbon-carbon bonds. here we demonstrate that members of the recently discovered genus methylocella are an exception to this. these bacteria are able to use as their sole energy source the one-carbon compounds methane and methanol, as well as the multicarbon compounds acetate, pyruvate, succinate, malate, and ethanol. to conclusively verify facultative growth, acetate and methane were used as model ... | 2005 | 15968078 |
| regulation of methane oxidation in the facultative methanotroph methylocella silvestris bl2. | the molecular regulation of methane oxidation in the first fully authenticated facultative methanotroph methylocella silvestris bl2 was assessed during growth on methane and acetate. problems of poor growth of methylocella spp. in small-scale batch culture were overcome by growth in fermentor culture. the genes encoding soluble methane monooxygenase were cloned and sequenced, which revealed that the structural genes for soluble methane monooxygenase, mmoxybzdc, were adjacent to two genes, mmor a ... | 2005 | 16238619 |
| aerobic methanotrophic bacteria of cold ecosystems. | this review summarizes the recent advances in understanding the ecophysiological role and structure-function features of methanotrophic bacteria living in various cold ecosystems. the occurrence of methanotrophs in a majority of psychrosphere sites was verified by direct measurement of their methane-utilizing activity, by electron microscopy and immunofluorescent observations, and analyses of specific signatures in cellular phospholipids and total dnas extracted from environmental samples. surpr ... | 2005 | 16329925 |
| {gamma}-glutamylmethylamide is an essential intermediate in the metabolism of methylamine by methylocella silvestris. | methylocella silvestris bl2, a facultative methane utilizer, can grow on monomethylamine (mma) as a sole carbon and nitrogen source. no activity of mma dehydrogenase was detectable. instead, this bacterium utilizes a methylated amino acid pathway (gamma-glutamylmethylamide [gma] and n-methylglutamate [nmg]) for mma metabolism. the activities of the two key enzymes in this pathway, gma synthetase and nmg dehydrogenase, were found when the bacterium was grown on mma. gma was detected by high-perfo ... | 2010 | 20472738 |
| complete genome sequence of the aerobic facultative methanotroph methylocella silvestris bl2. | methylocella silvestris bl2 is an aerobic methanotroph originally isolated from an acidic forest soil in germany. it is the first fully authenticated facultative methanotroph. it grows not only on methane and other one-carbon (c(1)) substrates, but also on some compounds containing carbon-carbon bonds, such as acetate, pyruvate, propane, and succinate. here we report the full genome sequence of this bacterium. | 2010 | 20472789 |
| acetate represses methane oxidation by methylocella silvestris in a peat soil microcosm. | methylocella are facultative methanotrophs that grow on methane and multi-carbon substrates such as acetate. acetate represses transcription of methane monooxygenase of m. silvestris in laboratory culture. dna-sip using (13)c- methane and (12)c-acetate, carried out with methylocella-spiked peat soil, showed that acetate also repressed methane oxidation by methylocella in environmental samples. | 2011 | 21515721 |
| the (d)evolution of methanotrophy in the beijerinckiaceae--a comparative genomics analysis. | the alphaproteobacterial family beijerinckiaceae contains generalists that grow on a wide range of substrates, and specialists that grow only on methane and methanol. we investigated the evolution of this family by comparing the genomes of the generalist organotroph beijerinckia indica, the facultative methanotroph methylocella silvestris and the obligate methanotroph methylocapsa acidiphila. highly resolved phylogenetic construction based on universally conserved genes demonstrated that the bei ... | 2013 | 23985741 |
| identification and characterization of trimethylamine n-oxide (tmao) demethylase and tmao permease in methylocella silvestris bl2. | methylocella silvestris, an alphaproteobacterium isolated from a forest soil, can grow on trimethylamine n-oxide (tmao) as a sole nitrogen source; however, the molecular and biochemical mechanisms underpinning its growth remain unknown. marker-exchange mutagenesis enabled the identification of several genes involved in tmao metabolism, including msil_3606, a permease of the amino acids-polyamine (apc) superfamily, and msil_3603, consisting of an n-terminal domain of unknown function (duf1989) an ... | 2014 | 25088783 |
| methylocella silvestris sp. nov., a novel methanotroph isolated from an acidic forest cambisol. | two strains of gram-negative, aerobic, non-pigmented, non-motile, rod-shaped, methane-oxidizing bacteria were isolated from an acidic forest cambisol near marburg, germany, and were designated as strains bl2(t) and a1. these bacteria were morphologically and phenotypically similar to methylocella palustris k(t). the cells possess a highly specific bipolar appearance. they lack the intracytoplasmic membranes common to all methane-oxidizing bacteria except methylocella, but contain a vesicular mem ... | 2003 | 13130000 |
| comparison of one- and two-dimensional liquid chromatography approaches in the label-free quantitative analysis of methylocella silvestris. | the proteome of the bacterium methylocella silvestris has been characterized using reversed phase ultra high pressure liquid chromatography (uplc) and two-dimensional reversed phase (high ph)-reversed phase (low ph) uplc prior to mass spectrometric analysis. variations in protein expression levels were identified with the aid of label-free quantification in a study of soluble protein extracts from the organism grown using methane, succinate, or propane as a substrate. the number of first dimensi ... | 2012 | 22871101 |
| trace-gas metabolic versatility of the facultative methanotroph methylocella silvestris. | the climate-active gas methane is generated both by biological processes and by thermogenic decomposition of fossil organic material, which forms methane and short-chain alkanes, principally ethane, propane and butane. in addition to natural sources, environments are exposed to anthropogenic inputs of all these gases from oil and gas extraction and distribution. the gases provide carbon and/or energy for a diverse range of microorganisms that can metabolize them in both anoxic and oxic zones. ae ... | 2014 | 24776799 |
| methylocella: a gourmand among methanotrophs. | a recent article in nature describes the ability of methylocella silvestris to grow simultaneously on methane and longer chain alkanes, something never before observed in the microbial world. it adds to a growing list of unique metabolic traits that distinguish methylocella from any other bacterium. | 2014 | 24874563 |
| biological methanol production by a type ii methanotroph methylocystis bryophila. | methane (ch₄) is the most abundant component in natural gas. to reduce its harmful environmental effect as a greenhouse gas, ch₄ can be utilized as a low-cost feed for the synthesis of methanol by methanotrophs. in this study, several methanotrophs were examined for their ability to produce methanol from ch₄; including methylocella silvestris, methylocystis bryophila, methyloferula stellata, and methylomonas methanica. among these methanotrophs, m. bryophila exhibited the highest methanol produc ... | 2016 | 26838340 |
| o2 -independent demethylation of trimethylamine n-oxide by tdm of methylocella silvestris. | bacterial trimethylamine n-oxide (tmao) demethylase, tdm, carries out an unusual oxygen-independent demethylation reaction, resulting in the formation of dimethylamine and formaldehyde. in this study, site-directed mutagenesis, homology modelling and metal analyses by inorganic mass spectrometry have been applied to gain insight into metal stoichiometry and underlying catalytic mechanism of tdm of methylocella silvestris bl2. herein, we demonstrate that active tdm has 1 molar equivalent of zn(2+ ... | 2016 | 27644004 |
| Bacterial flavin-containing monooxygenase is trimethylamine monooxygenase. | Flavin-containing monooxygenases (FMOs) are one of the most important monooxygenase systems in Eukaryotes and have many important physiological functions. FMOs have also been found in bacteria; however, their physiological function is not known. Here, we report the identification and characterization of trimethylamine (TMA) monooxygenase, termed Tmm, from Methylocella silvestris, using a combination of proteomic, biochemical, and genetic approaches. This bacterial FMO contains the FMO sequence m ... | 2011 | 22006322 |
| development of a system for genetic manipulation of the facultative methanotroph methylocella silvestris bl2. | an understanding of the metabolism and metabolic regulation of the facultative methanotroph methylocella silvestris bl2 is required to understand its role in methane oxidation in the environment, and methods for genetics manipulation are essential tools in these investigations. in addition, the ability to engineer the metabolic capabilities of m. silvestris may well have useful biotechnological applications. we describe a simple and effective method of genetic manipulation for this organism whic ... | 2011 | 21419918 |
| a comparison of labeling and label-free mass spectrometry-based proteomics approaches. | the proteome of the recently discovered bacterium methylocella silvestris has been characterized using three profiling and comparative proteomics approaches. the organism has been grown on two different substrates enabling variations in protein expression to be identified. the results obtained using the experimental approaches have been compared with respect to number of proteins identified, confidence in identification, sequence coverage and agreement of regulated proteins. the sample preparati ... | 2009 | 19435289 |