| identification of a kunitz inhibitor from albizzia kalkora and its inhibitory effect against pest midgut proteases. | a purification protocol, involving water extraction, ammonium sulfate precipitation, sepharose 4b-trypsin affinity and fplc superdex g-75 chromatography, was employed to isolate a trypsin inhibitor from albizzia kalkora seeds. the inhibitor, which had a molecular mass of 19,768.23 da, consisted of two disulfide-linked polypeptide chains with approximate molecular mass of 15.5 and 4.5 kda, respectively. it was stable from ph 2-12 for 24 h, whereas it was unstable either above 80 degrees c for 10 ... | 2008 | 18368297 |
| mesorhizobium albiziae sp. nov., a novel bacterium that nodulates albizia kalkora in a subtropical region of china. | a novel mesorhizobium group associated with albizia kalkora [wang et al. (2006), syst appl microbiol 29, 502-517] was further characterized. the seven strains in this group showed similar protein patterns and were different from defined mesorhizobium species in sds-page of whole-cell proteins. the representative strain ccbau 61158(t) formed a novel mesorhizobium lineage in phylogenetic analyses of 16s rrna, atpd, glnii and nifh genes. however, its nodc gene sequence was more similar to that of r ... | 2007 | 17551028 |