| female sex pheromone of cameraria ohridella desch. and dim. (lepidoptera: gracillariidae): structure confirmation, synthesis and biological activity of (8e,10z)-8,10-tetradecadienal and some analogues. | mass spectrometric investigations confirmed the structure of the female produced sex pheromone of the horse-chestnut leafminer cameraria ohridella desch. and dim. to be (8e,ioz)-8,10-tetradecadienal. pure samples, prepared in a straightforward synthesis, were highly attractive in field tests and proved to be suitable for monitoring of flight activities and population dynamics. in mixtures with the synthetic pheromone, analogues like 9-tridecynal and 7-dodecynyl formate were shown to reduce trap ... | 2002 | 12241006 |
| sex pheromone of horse-chestnut leafminer camneraria ohridella and its use in a pheromone-based monitoring system. | gas chromatography combined with electroantennographic detection (gc-ead), electroantennography (eag), and wind-tunnel and field experiments were used to reinvestigate the composition of cameraria ohridella (lepidoptera, gracillariidae, lithocolletinae) sex pheromone. the gc-ead experiments showed one ead-active area corresponding to the major pheromone component. (8e,10z)-tetradeca-8,10-dienal. the eag experiments proved that (9e)-tetracedecenal and stereoisomers of (8e,10z)-tetradeca-8,10-dien ... | 2003 | 12737265 |
| antibacterial and antifungal lysozyme-type activity in cameraria ohridella pupae. | lysozyme-type antibacterial and antifungal activity in pupae of cameraria ohridella was studied. activity against micrococcus luteus and bacillus megaterium was detected in pupae extract. also antifungal activity from c. ohridella pupae extract directed against saccharomyces cerevisiae strain w 303 was shown. during immunoblotting two bands in pupae extract, with molecular mass of about 15 and 28 kda were recognized by antibodies directed against hewl. after acid electrophoresis followed by bioa ... | 2005 | 16169556 |
| residues of diflubenzuron on horse chestnut (aesculus hippocastanum) leaves and their efficacy against the horse chestnut leafminer, cameraria ohridella. | residues of the insect growth regulator diflubenzuron were quantified on horse chestnut (aesculus hippocastanum l.) leaves treated with a diflubenzuron 480 g litre(-1) sc, dimilin. to analyse the samples, an analytical procedure was developed involving a simple extraction step followed by high-performance liquid chromatography on an octadecyl-modified silica column with methanol + 0.01 m ammonium acetate mobile phase. the results showed diflubenzuron to be highly stable on horse chestnut leaves; ... | 2006 | 16475222 |
| possibilities to control the horse chestnut leaf miner (cameraria ohridella) in urban environments. | the continuous epidemic infestation of horse chestnuts by c. ohridella is a serious aesthetic and economic problem in urban environments. the aim of the presented studies was therefore to develop control measures which meet the special needs of urban plant protection. removal of infested leaves in autumn and appropriate disposal of the leaflitter is the only practicable measure to reduce infestation levels so far. another two methods, which are currently in an experimental stage, had an impact o ... | 2005 | 16628897 |
| volatile emissions from aesculus hippocastanum induced by mining of larval stages of cameraria ohridella influence oviposition by conspecific females. | larval stages of the horse chestnut leafminer cameraria ohridella can completely destroy the surface of horse chestnut leaves, aesculus hippocastanum. this study investigated the effect of the degree of leaf browning caused by the insect's larvae on olfactory detection, aggregation, and oviposition of c. ohridella adults. the influence of a. hippocastanum flower scent on oviposition of the first generation was also evaluated. utilizing gas chromatography coupled with parallel detection by mass s ... | 2006 | 17001531 |
| bidirectional, organocatalytic synthesis of lepidopteran sex pheromones. | shuffling of two simple building blocks and a regioselective transfer hydrogenation allow for the rapid synthesis of a small collection of lepidopteran sex pheromones, e.g., 8e,10z-tetradeca-8,10-dienal 5c, from the horse chestnut leafminer (cameraria ohridella). | 2007 | 17221987 |
| bayesian analysis of the species-specific lengthening of the growing season in two european countries and the influence of an insect pest. | a recent lengthening of the growing season in mid and higher latitudes of the northern hemisphere is reported as a clear indicator for climate change impacts. using data from germany (1951-2003) and slovenia (1961-2004), we study whether changes in the start, end, and length of the growing season differ among four deciduous broad-leaved tree species and countries, how the changes are related to temperature changes, and what might be the confounding effects of an insect attack. the functional beh ... | 2008 | 17805581 |
| two methods of assessing the mortality factors affecting the larvae and pupae of cameraria ohridella in the leaves of aesculus hippocastanum in switzerland and bulgaria. | the horse-chestnut leaf miner, cameraria ohridella, is an invasive alien species defoliating horse-chestnut, a popular ornamental tree in europe. this paper presents quantitative data on mortality factors affecting larvae and pupae of the leaf miner in switzerland and bulgaria, both in urban and forest environments. two sampling methods were used and compared: a cohort method, consisting of the surveying of pre-selected mines throughout their development, and a grab sampling method, consisting o ... | 2007 | 17916263 |
| sequential sampling plan for cameraria ohridella (lepidoptera: gracillariidae) on horse chestnut tree. | a fixed precision sequential sampling plan for estimating the density of the horse chestnut, aesculus hippocastanum l., leafminer cameraria ohridella deschka & dimic (lepidoptera: gracillariidae) was developed. data were collected from 2002 to 2004 in turin, northwestern italy, with the aim of developing a sampling strategy for estimating populations of c. ohridella mines. taylor's power law was used as a regression model. sampling parameters were estimated from 216 data sets, and an additional ... | 2007 | 18232410 |
| evaluation of the community of native eulophid parasitoids on cameraria ohridella deschka and dimic in urban areas. | the parasitoid complex associated with the exotic leafminer cameraria ohridella deschka and dimic (lepidoptera: gracillariidae), which attacks horse chestnut (aesculus hippocastanum l.), was studied in the urban environment of turin (northern italy). the studies were carried out over 5 yr after the first detection of the pest in our region in 1999. to evaluate parasitism, 438,029 leaf mines were examined over the 5-yr period, of which 29,033 were found to be parasitized (6.6%). also, ornamental ... | 2007 | 18284739 |
| native parasitoids and their potential to control the invasive leafminer, cameraria ohridella desch. & dim. (lep.: gracillariidae). | in spite of the fact that since the end of the eighties, the horse chestnut leafminer, cameraria ohridella, has established itself throughout europe, native predators such as ants and birds are not attuned to this neozoic species. in contrast, several parasitic wasp species already started to exploit the invasive horse chestnut leafminer, but until now parasitation rates are quite low, mainly because of asynchrony in the lifecycles of parasitoids and host. only the removal of leaf litter, in whi ... | 2008 | 18294419 |
| pathogenicity of entomopathogenic fungi on hibernating pupae of cameraria ohridella deschka & dimic 1986 (lepidoptera, gracillariidae). part 1: pathogenicity against the naked pupa. | strains from paecilomyces fumosoroseus, lecanicillium muscarium, metarhizium anisopliae and beauveria bassiana were examined in standardized biotest to control the horse-chestnut leaf miner (cameraria ohridella) in her pupal stage in winter. the fungi were pathogenic against the hibernating pupae of cameraria ohridella at dose of 1.9 x 10(7) conidia/ml. they were aggressive, led to infection, death and mouldiness of naked pupae. even at low temperature of 5 degrees c and 12 degrees c. l. muscari ... | 2007 | 18399468 |
| pathogenicity of entomopathogenic fungi on hibernating pupae of cameraria ohridella deschka & dimic 1986 (lepidoptera, gracillariidae). part 2: efficacy of entomopathogenic fungi against pupa in her pupal cell. | the naked pupae of chestnut leafminer cameraria ohridella are susceptible for entomopathogenic fungi paecilomyces fumosoroseus strain p6; lecanicillium muscarium strain v24; metarhizium anisopliae strain m72 and beauveria bassiana strain b412. their activity at low temperature is advantageous especially for the use in the winter season. (richter et al., 2007a). therefore the possibility of infection should be examined in biotest for pupae in their pupal cells on leaf of chestnut at conditions li ... | 2007 | 18399469 |
| perspectives for the biological control of cameraria ohridella. | the horse chestnut leaf-miner, cameraria ohridella deschka et dimic (lepidoptera, gracillariidae) is a serious invasive pest of aesculus hippocastanum in europe. the larvae of this species feed on leaf parenchyma and can reduce the tree growth. we studied the impact of parasitoids on c. ohridella in the czech republic and also searched for entomopathogenic fungi associated with this pest. the results showed that the rate of parasitism varied between 5% and 15% in most cases. the most parasitized ... | 2007 | 18399483 |
| mitochondrial and microsatellite dna markers reveal a balkan origin for the highly invasive horse-chestnut leaf miner cameraria ohridella (lepidoptera, gracillariidae). | biological invasions usually start with a small number of founder individuals. these founders are likely to represent a small fraction of the total genetic diversity found in the source population. our study set out to trace genetically the geographical origin of the horse-chestnut leafminer, cameraria ohridella, an invasive microlepidopteran whose area of origin is still unkown. since its discovery in macedonia 25 years ago, this insect has experienced an explosive westward range expansion, pro ... | 2009 | 19627490 |
| aesculus pavia foliar saponins: defensive role against the leafminer cameraria ohridella. | recently, the leafminer cameraria ohridella deschka & dimic has caused heavy damage to the white-flowering horse chestnut in europe. among the aesculus genus, a. pavia l. hbt genotype, characterised by red flowers, showed an atypical resistance towards this pest. its leaves, shaken in water, originated a dense foam, indicating the presence of saponins, unlike the common horse chestnut tree. the aim was to isolate and identify these leaf saponins and test their possible defensive role against c. ... | 2010 | 20217891 |
| persistence of the entomopathogenic fungus lecanicillium muscarium zare & gams under ambient conditions in the field. | positive results of laboratory trials, which proofed the effectiveness of the entomopathogenic fungus l. muscarium against endophytic larvae of the horse chestnut leafminer moth cameraria ohridella deschka et dimic, led to outdoor trials. one aspect of these trials was to determine the persistence of the fungus, which was used as commercial product mycotal (koppert, nl) and as strain v24 from the section phytomedicine. in different variants several spore concentrations and the influence of an oi ... | 2009 | 20222590 |
| digestive enzymes activity in larvae of cameraria ohridella (lepidoptera: gracillariidae). | this article presents the activity of carbohydratases and proteases in the midgut of cameraria ohridella larvae--an oligophagous pest whose preferred feeding is horse chestnuts leaves. optimal media ph of the assayed enzymes were similar to those of other lepidopterans. relatively high amylase activity, as well as maltase and sucrase activities, indicates that starch and sucrose are the main digested saccharides. trehalase activity was similar to that described in other lepidopterans. activities ... | 2010 | 20965442 |
| an attempt to control cameraria ohridella using an attract-and-kill technique. | this study estimates the efficacy of an attract-and-kill (a&k) technique to control the horse chestnut leaf miner, cameraria ohridella deschka and dimic (lepidoptera, gracillariidae), an invasive insect pest of the horse chestnut, aesculus hippocastanum l. (hippocastanaceae). the a&k formulation was dispensed as 50 μl droplets of paste-like matrix, containing c. ohridella sex pheromone, (8e,10z)-tetradeca-8,10-dienal (85% + pure; 0.16% w/w) and a fast acting contact toxicant, pyrocides (94% pure ... | 2011 | 21654920 |
| laboratory evaluation of isaria fumosorosea against spodoptera littoralis. | isaria fumosorosea (syn. paecilomyces fumosoroseus) is potentially useful for the biological control of economically important agricultural and forest insect pests. we evaluated efficacy of two strains of this entomopathogenic fungus against last instar larvae and pupae of egyptian cotton leafworm, spodoptera littoralis. the first strain was apopka 97 which is an active ingredient of commercial biopesticide preferal wg (biobest, belgium). the second strain was isolated from cameraria ohridella a ... | 2012 | 23885438 |
| the role of geography and host abundance in the distribution of parasitoids of an alien pest. | chalcid wasps (hymenoptera: chalcidoidea) are probably the most effective and abundant parasitoids of the horse chestnut leaf miner (cameraria ohridella), an alien pest in europe that lacks specialized enemies. we studied how the species richness and abundance of chalcids are influenced by altitude, direction of an alien spread and host abundance of c. ohridella. we quantified the numbers and species richness of chalcid wasps and the numbers of c. ohridella that emerged from horse chestnut (aesc ... | 2016 | 26819849 |
| the content of phenolic compounds in leaf tissues of white (aesculus hippocastanum l.) and red horse chestnut (aesculus carea h.) colonized by the horse chestnut leaf miner (cameraria ohridella deschka & dimić). | normally, plant phenolics are secondary metabolites involved in the defense mechanisms of plants against fungal pathogens. therefore, in this study we attempted to quantify and characterize phenolic compounds in leaves of white and red horse chestnut with leaf miner larvae before and after cameraria ohridella attack. a total of 17 phenolic compounds belonging to the hydroxycinnamic acid, flavan-3-ols and flavonol groups were identified and quantified in white and red horse chestnut leaf extracts ... | 2014 | 25225723 |
| the success of the horse-chestnut leaf-miner, cameraria ohridella, in the uk revealed with hypothesis-led citizen science. | citizen science is an increasingly popular way of undertaking research and simultaneously engaging people with science. however, most emphasis of citizen science in environmental science is on long-term monitoring. here, we demonstrate the opportunities provided by short-term hypothesis-led citizen science. in 2010, we ran the 'conker tree science' project, in which over 3500 people in great britain provided data at a national scale of an insect (horse-chestnut leaf-mining moth, cameraria ohride ... | 2014 | 24465973 |
| leafminers help us understand leaf hydraulic design. | leaf hydraulics of aesculus hippocastanum l. were measured over the growing season and during extensive leaf mining by the larvae of an invasive moth (cameraria ohridella deschka et dimic) that specifically destroy the palisade tissue. leaves showed seasonal changes in hydraulic resistance (r(lamina)) which were related to ontogeny. after leaf expansion was complete, the hydraulic resistance of leaves and the partitioning of resistances between vascular and extra-vascular compartments remained u ... | 2010 | 20199625 |
| ancient expansion of the hox cluster in lepidoptera generated four homeobox genes implicated in extra-embryonic tissue formation. | gene duplications within the conserved hox cluster are rare in animal evolution, but in lepidoptera an array of divergent hox-related genes (shx genes) has been reported between pb and zen. here, we use genome sequencing of five lepidopteran species (polygonia c-album, pararge aegeria, callimorpha dominula, cameraria ohridella, hepialus sylvina) plus a caddisfly outgroup (glyphotaelius pellucidus) to trace the evolution of the lepidopteran shx genes. we demonstrate that shx genes originated by t ... | 2014 | 25340822 |
| a burst of mirna innovation in the early evolution of butterflies and moths. | micrornas (mirnas) are involved in posttranscriptional regulation of gene expression. because several mirnas are known to affect the stability or translation of developmental regulatory genes, the origin of novel mirnas may have contributed to the evolution of developmental processes and morphology. lepidoptera (butterflies and moths) is a species-rich clade with a well-established phylogeny and abundant genomic resources, thereby representing an ideal system in which to study mirna evolution. w ... | 2015 | 25576364 |
| digestive enzymes activity in subsequent generations of cameraria ohridella larvae harvested from horse chestnut trees after treatment with imidacloprid. | in the present study we describe the effect of chloronicotinoid pesticide (imidacloprid) on the digestive enzymes activity of the cameraria ohridella larvae after lasting 1 year sublethal exposure to imidacloprid pesticide. caterpillars - l4 stage (fourth instar, hyperphagic tissue-feeding phase) - were collected from chemically protected white horse chestnut trees 1 year after imidacloprid treatment, and compared with caterpillars collected from non-treated trees in a previous study. enzymes ac ... | 2013 | 24238283 |
| direct and unbiased information recovery from liquid chromatography-mass spectrometry raw data for phenotype-differentiating metabolites based on screening window coefficient of ion currents. | a reworking of a data mining strategy, in which statistical treatment of raw data from liquid chromatography-mass spectrometry (lc-ms) precedes recognition of chromatographic peaks, is presented. in this algorithm the tr-m/z plane of lc-ms data is divided into equal-sized segments of twelve seconds by one m/z unit each, and the total ion currents in corresponding segments as specified by the tr-m/z pair from multiple lc-ms runs are evaluated to generate mean ion currents (μ) and standard deviati ... | 2013 | 24004415 |
| within and between seasonal changes of detoxifying capabilities of cameraria ohridella (lepidoptera : gracillariidae) larvae. | cameraria ohridella is an invasive leaf miner, a severe pest of horse chestnut trees. chemical control needs recognition of intrinsic metabolic capability to cope with external stressing factors. our tasks were to check annual effects of generation, and host tree age on detoxifying abilities of the last larval stage. activities of cat, sod, gstpx, gst care and ache were assayed in the midgut of larvae from two localities during 3 years and three generations, annually. activities of gstpx and gst ... | 2014 | 23199632 |
| host tracking or cryptic adaptation? phylogeography of pediobius saulius (hymenoptera, eulophidae), a parasitoid of the highly invasive horse-chestnut leafminer. | classical biological control is often advocated as a tool for managing invasive species. however, accurate evaluations of parasitoid species complexes and assessment of host specificity are impeded by the lack of morphological variation. here, we study the possibility of host races/species within the eulophid wasp pediobius saulius, a pupal generalist parasitoid that parasitize the highly invasive horse-chestnut leaf-mining moth cameraria ohridella. we analysed the population genetic structure, ... | 2012 | 25568046 |
| studying nanostructured nipple arrays of moth eye facets helps to design better thin film solar cells. | nipples on the surface of moth eye facets exhibit almost perfect broadband anti-reflection properties. we have studied the facet surface micro-protuberances, known as corneal nipples, of the chestnut leafminer moth cameraria ohridella by atomic force microscopy, and simulated the optics of the nipple arrays by three-dimensional electromagnetic simulation. the influence of the dimensions and shapes of the nipples on the optics was studied. in particular, the shape of the nipples has a major influ ... | 2012 | 22155981 |