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genetic diversity and ecological evaluation of fluorescent pseudomonads isolated from the leaves and roots of potato plants.a total of 828 isolates of fluorescent pseudomonads (fps) were obtained from the leaves (305 isolates) and roots (523 isolates) of potato plants grown in different geographical locations in japan, and 16s rrna gene sequences of 776 isolates were successfully determined by direct pcr sequencing. clustering analysis (≥99% identity) identified 13 and 26 operational taxonomic units (otus) for leaf- and root-associated fps, respectively, and 29 otus were identified in the phytosphere of potato plants ...201222791043
stability of a promiscuous plasmid in different hosts: no guarantee for a long-term relationship.broad-host-range (bhr) incp-1 plasmids have the ability to transfer between and replicate in nearly all species of the alpha-, beta- and gammaproteobacteria, but surprisingly few data are available on the stability of these plasmids in strains within their host range. moreover, even though molecular interactions between the bacterial host and its plasmid(s) exist, no systematic study to date has compared the stability of the same plasmid among different hosts. the goal of this study was to exami ...200717259616
draft genome sequence of pseudomonas koreensis ci12, a bacillus cereus "hitchhiker" from the soybean rhizosphere.pseudomonas koreensis ci12 was coisolated with bacillus cereus from a root of a soybean plant grown in a field in arlington, wi. here, we report the draft genome sequence of p. koreensis ci12 obtained by illumina sequencing.201728663293
exploring characteristics of bioelectricity generation and dye decolorization of mixed and pure bacterial cultures from wine-bearing wastewater treatment.this study uncovered microbial characteristics of bioelectricity generation and dye decolorization in single-chamber microbial fuel cells (mfcs) using activated sludge for wine-containing wastewater treatment. phylogenetic tree analysis on 16s rrna gene fragments indicated that the predominant strains on anodic biofilm in acclimatized mfcs were gamma-proteobacteria aeromonas punctata niu-p9, pseudomonas plecoglossicida niu-y3, pseudomonas koreensis niu-x8, acinetobacter junii niu-y8, stenotropho ...201120859654
genome sequence of pseudomonas koreensis crs05-r5, an antagonistic bacterium isolated from rice paddy field. 201627877162
potential use of pseudomonas koreensis agb-1 in association with miscanthus sinensis to remediate heavy metal(loid)-contaminated mining site soil.endophytic bacteria have the potential to promote plant growth and heavy metal(loid) (hm) removal from contaminated soil. pseudomonas koreensis agb-1, isolated from roots of miscanthus sinensis growing in mine-tailing soil, exhibited high tolerance to hms and plant growth promoting traits. transmission electron microscope (tem) analysis revealed that agb-1 sequestered hms extracellularly and their accumulation was visible as dark metal complexes on bacterial surfaces and outside of the cells. dn ...201525575343
[effects of actinomycetes agent on ginseng growth and rhizosphere soil microflora].taking the ginseng in xiao xing' an mountains of northeast china as test object, this paper studied the effects of applying streptomyces pactum (act12) on ginseng growth and on the soil microflora in root zone and root surface. after treated with act12, the yield and quality of ginseng' s medicinal part improved, the induced enzyme activities in leaves and the root activity increased, and the numbers and proportions of soil bacteria and actinomycetes increased significantly while those of soil f ...201324380350
water treatment using activated carbon supporting silver and magnetite.recent efforts in water purification have led to the development of novel materials whose unique properties can offer effective biocidal capabilities with greater ease of use and at lower cost. in this study, we introduce a novel procedure for the preparation of activated carbon (charcoal) composite in which magnetite and silver are incorporated (mcag); we also describe the use of this material for the disinfection of surface water. the formation process of magnetic mcag composite was studied us ...201223109597
efficacy of lactoferricin b in controlling ready-to-eat vegetable spoilage caused by pseudomonas spp.the microbial content of plant tissues has been reported to cause the spoilage of ca. 30% of chlorine-disinfected fresh vegetables during cold storage. the aim of this work was to evaluate the efficacy of antimicrobial peptides in controlling microbial vegetable spoilage under cold storage conditions. a total of 48 bacterial isolates were collected from ready-to-eat (rte) vegetables and identified as belonging to acinetobacter calcoaceticus, aeromonas media, pseudomonas cichorii, pseudomonas flu ...201526453993
distribution of pseudomonas species in a dairy plant affected by occasional blue discoloration.during 2010 many cases of discoloration in mozzarella, popularly termed as blue mozzarella, have been reported to the attention of public opinion. causes of the alteration were bacteria belonging to the genus pseudomonas. the strong media impact of such cases has created confusion, not only among consumers, but also among experts. in order to help improving the knowledge on microbial ecology of this microorganism a study has been set up with the collaboration of a medium-sized dairy plant produc ...201427800364
recovery of pseudomonas koreensis from eye lesions in golden mahseer, tor putitora (hamilton, 1822) in uttarakhand, india. 201424033716
pseudomonas granadensis sp. nov., a new bacterial species isolated from the tejeda, almijara and alhama natural park, granada, spain.during the course of screening bacterial isolates as sources of as-yet unknown bioactive compounds with pharmaceutical applications, a chemo-organotrophic, gram-negative bacterium was isolated from a soil sample taken from the tejeda, almijara and alhama natural park, granada, spain. strain f-278,770(t) was oxidase- and catalase-positive, aerobic, with a respiratory type of metabolism with oxygen as the terminal electron acceptor, non-spore-forming and motile by one polar flagellum, although som ...201525410940
composition of the bacterial population of refrigerated beef, identified with direct 16s rrna gene analysis and pure culture technique.the composition of the dominating population of freshly cut beef, and beef stored at 4 degrees c for 8 d, was studied by direct analysis of the 16s rrna gene (pcr amplification, cloning and sequencing) and compared with pure culture technique where the isolates picked from the viable plate count were identified by sequencing of the 16s rrna gene. the composition of the bacterial population was recorded at two different time points, at the start when the viable plate count of the meat was 4 x 10( ...200717765348
pseudomonas baetica sp. nov., a novel fish pathogen isolated from wedge sole, dicologoglossa cuneata (moreau).five gram negative bacterial isolates recovered from an outbreak occurred in march 2006 in huelva, spain, affecting adult diseased cultured wedge sole (dicologoglossa cuneata), were phenotypically and genotypically characterized in order to clarify its taxonomic position. on the basis of 16s rrna gene sequence analysis isolates were included in the genus pseudomonas, within the p. fluorescens-related species group, being their closest relatives the p. jessenii and p. koreensis subgroups. the hig ...201121642488
pseudomonas koreensis sp. nov., pseudomonas umsongensis sp. nov. and pseudomonas jinjuensis sp. nov., novel species from farm soils in korea.among pseudomonas strains isolated from korean agricultural soils, four strains (ps 9-14 group: ps 1-2, ps 1-10, ps 5-5 and ps 9-14t) from the suwon, goesan and samchok regions, three strains (ps 3-10 group: ps 2-22, ps 3-1 and ps 3-10t) from umsong region and four strains (pss 26 group: pss 14, pss 25, pss 26t and pss 27) from jinju region were identified as three independent groups on the basis of 16s rdna sequence analysis. while, on the basis of 16s rdna sequence analysis, ps 9-14t and ps 3- ...200312656147
pseudomonas helmanticensis sp. nov., isolated from forest soil.a bacterial strain, oha11(t), was isolated during the course of a study of phosphate-solubilizing bacteria occurring in a forest soil from salamanca, spain. the 16s rrna gene sequence of strain oha11(t) shared 99.1% similarity with respect to pseudomonas baetica a390(t), and 98.9% similarity with the type strains of pseudomonas jessenii, pseudomonas moorei, pseudomonas umsongensis, pseudomonas mohnii and pseudomonas koreensis. the analysis of housekeeping genes rpob, rpod and gyrb confirmed its ...201424744015
mass spectrometry identification of alkyl-substituted pyrazines produced by pseudomonas spp. isolates obtained from wine corks.we investigated the pyrazine production of 23 pseudomonas isolates obtained from cork in order to assess their implications in off-flavour development. off-flavour development in cork stoppers is a crucial process in maintaining the high quality of some wines. pyrazine production was analyzed by headspace solid-phase-microextraction (hs-spme) and gas chromatography coupled with mass spectrometry (gc-ms). five out of the 23 isolates, i.e. pseudomonas koreensis tca20, pseudomonas palleroniana tca1 ...201223497899
pseudomonas moraviensis sp. nov. and pseudomonas vranovensis sp. nov., soil bacteria isolated on nitroaromatic compounds, and emended description of pseudomonas asplenii.two strains of gram-negative bacteria isolated from soil by selective enrichment with nitroaromatics were subjected to a polyphasic taxonomic study. on the basis of 16s rrna gene sequence analysis, the two strains were found to belong to the genus pseudomonas, within the gammaproteobacteria. strain 1b4t shared the highest sequence similarity with pseudomonas koreensis dsm 16610t (99.5%) and pseudomonas jessenii ccm 4840t (99.3%), and strain 2b2t with pseudomonas asplenii dsm 17133t (98.9%), pseu ...200617082407
insights into the fecal microbiota of captive iberian lynx (lynx pardinus).the iberian lynx (lynx pardinus) is an endangered species restricted to several areas of spain and portugal. its low genetic diversity likely provokes immune depression and high susceptibility to infectious diseases. the intestinal microbiota is closely related to host health and nutrition. in order to contribute to the knowledge of the iberian lynx intestinal microbiota, fecal microbiota of captive specimens from two breeding centers ("la olivilla" and "el acebuche"), located in southern spain, ...201728581020
genetic diversity of nitrogen-fixing and plant growth promoting pseudomonas species isolated from sugarcane rhizosphere.the study was designed to isolate and characterize pseudomonas spp. from sugarcane rhizosphere, and to evaluate their plant- growth- promoting (pgp) traits and nitrogenase activity. a biological nitrogen-fixing microbe has great potential to replace chemical fertilizers and be used as a targeted biofertilizer in a plant. a total of 100 isolates from sugarcane rhizosphere, belonging to different species, were isolated; from these, 30 isolates were selected on the basis of preliminary screening, f ...201728769881
arsenic-tolerant plant-growth-promoting bacteria isolated from arsenic-polluted soils in south korea.the janghang smelter in chungnam, south korea started in 1936 was subsequently shutdown in 1989 due to heavy metal (loid) pollution concerns in the vicinity. thus, there is a need for the soil in the area to be remediated to make it usable again especially for agricultural purposes. the present study was conducted to exploit the potential of arsenic (as)-tolerant bacteria thriving in the vicinity of the smelter-polluted soils to enhance phytoremediation of hazardous as. we studied the genetic an ...201424737020
epiphytic marine pigmented bacteria: a prospective source of natural antioxidants.awareness on antioxidants and its significance in human healthcare has increased many folds in recent time. increased demand requisite on welcoming newer and alternative resources for natural antioxidants. seaweed associated pigmented bacteria screened for its antioxidant potentials reveals 55.5% of the organisms were able to synthesize antioxidant compounds. dpph assay showed 20% of the organisms to reach a antioxidant zone of 1 cm and 8.3% of the strains more than 3 cm. pseudomonas koreensis ( ...201526221086
phosphate solubilizing and indole-3-acetic acid producing bacteria from the soil of garhwal himalaya aimed to improve the growth of rice.in the present study, soil bacteria from rainfed agriculture field of garhwal himalaya, just prior to sowing of summer crop, were isolated and initially tested for solubilization of inorganic phosphate, production of indole acetic acid (iaa) and siderophore. two bacterial isolates, having efficient p- solubilizing activity in solid medium, were identified using 16s rrna sequence analysis as pseudomonas koreensis strainyb1 arthrobacter nitroguajacolicus strainyb3 and three bacterial isolates, pro ...201526536808
a simple strategy for investigating the diversity and hydrocarbon degradation abilities of cultivable bacteria from contaminated soil.the use of indigenous bacterial strains is a valuable bioremediation strategy for cleaning the environment from hydrocarbon pollutants. the isolation and selection of hydrocarbon-degrading bacteria is therefore crucial for obtaining the most promising strains for site decontamination. two different media, a minimal medium supplemented with a mixture of polycyclic aromatic hydrocarbons and a ms medium supplemented with triphenyltetrazolium chloride, were used for the isolation of bacterial strain ...201323386320
characterization of an extracellular lipase by pseudomonas koreensis bk-l07 isolated from soil.screening using spirit blue agar revealed that strain bk-l07 had the highest lipase activity. furthermore, the isolated strain was identified as pseudomonas sp. based on morphological, physiological, biochemical, and molecular analyses. the 16s rrna gene sequence of strain bk-l07 shared a high similarity with that of pseudomonas koreensis (99%). the nutritional conditions and physicochemical properties were influenced by p. koreensis bk-l07. the maximum lipase production was obtained in tryptic ...201424274015
characterization of a novel biosurfactant producing pseudomonas koreensis lineage that is endemic to cuatro ciénegas basin.the aim of this work is the taxonomic characterization of three biosurfactant-producing bacterial isolates from the churince system at cuatro ciénegas basin (ccb) in the mexican state of coahuila, and the study of the possible role of biosurfactant production in their ecology and evolution. we determined that these isolates belong to a pseudomonas koreensis lineage endemic to ccb, using standard taxonomical techniques, phylogenetic analysis of three chromosomal loci and phenotypic characterizati ...201121592712
pseudomonas kribbensis sp. nov., isolated from garden soils in daejeon, korea.two bacterial strains, 46-1 and 46-2(t), were isolated from garden soil. these strains were observed to be aerobic, gram-stain negative, rod-shaped, non-spore-forming, motile and catalase and oxidase positive. phylogenetic analysis based on 16s rrna gene sequences showed that the two strains shared 100 % sequence similarity with each other and belong to the genus pseudomonas in the class gammaproteobacteria. the concatenated 16s rrna, gyrb, rpob and rpod gene sequences further confirmed that the ...201627460204
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