| serological comparison of tospovirus isolates from taiwan and india with impatiens necrotic spot virus and different tomato spotted wilt virus isolates. | tospovirus isolates from tomato and watermelon in taiwan and from peanut in india reacted neither with antibodies to tomato spotted wilt virus (tswv), nor with antibodies to impatiens necrotic spot virus (insv), in elisa and in electroblot immunoassays. additionally, all three tospovirus isolates had an n protein with a molecular weight of 32 kda as compared to 29 kda for the n protein to tswv and insv. the observed physicochemical and serological differences suggest the generation of a new, thi ... | 1993 | 7686004 |
| resistance to tospoviruses in nicotiana benthamiana transformed with the n gene of tomato spotted wilt virus: correlation between transgene expression and protection in primary transformants. | nicotiana benthamiana was transformed with the nucleoprotein (n) gene of an italian isolate of tomato spotted wilt virus (tswv). forty-five t1 primary transformant lines were analyzed for the expression of n protein and for resistance to tswv and three other tospoviruses: impatiens necrotic spot virus (insv), groundnut bud necrosis virus (gbnv), and groundnut ringspot virus (grsv). thirteen of these lines were further characterized. resistance to all tswv isolates tested was found in two lines. ... | 1995 | 7772806 |
| the complete nucleotide sequence and genome organization of the m rna segment of peanut bud necrosis tospovirus and comparison with other tospoviruses. | the m rna of peanut bud necrosis virus (pbnv; synonym groundnut bud necrosis virus) is 4801 nucleotides in length. it comprised two orfs in an ambisense organization and terminal inverted repeats. the 3' large orf (3363 nucleotides in the virus-complementary strand) encoded a protein with a predicted size of 127.2 kda which was identified as the glycoprotein precursor (gp) of the g1 and g2 glycoproteins. a comparison of the deduced amino acid sequence of gp revealed 37% identity and 58-59% simil ... | 1996 | 8811036 |
| immunodiagnosis of groundnut and watermelon bud necrosis viruses using polyclonal antiserum to recombinant nucleocapsid protein of groundnut bud necrosis virus. | in vitro gene expression strategy was used for the production of polyclonal antiserum to the nucleocapsid protein (np) of groundnut bud necrosis virus (gbnv). the gbnv np gene from cowpea isolate was cloned into 6x his-tagged ua cloning vector and expressed in escherichia coli [m15] cells. the fusion protein was detected in insoluble fraction and was purified by using ni-nta agarose resin. the purified 6x his-fusion protein ( approximately 32 kda) was used for immunisation to produce a high titr ... | 2005 | 16095728 |
| nucleotide sequence of melon yellow spot virus m rna segment and characterization of non-viral sequences in subgenomic rna. | the nucleotide sequence of melon yellow spot virus (mysv) m rna segment was determined. the m rna segment contains one open reading frame (orf) encoding 308 amino acids (aa) in the sense orientation and another orf encoding 1,127 aa in the complementary orientation, which were homologous to the nsm protein and g1/g2 glycoprotein precursor (gp) protein, respectively. amino acid sequences identities with the other tospovirus suggested that mysv is closely related to groundnut bud necrosis virus an ... | 2006 | 16132174 |
| nucleotide sequence of the s and m rna segments of a groundnut bud necrosis virus isolate from vigna radiata in india. | nucleotide sequence of the s and m rna segments of a groundnut bud necrosis virus isolate from vigna radiata (mungbean isolate, gbnv-mb) was determined and compared with another isolate from arachis hypogaea (groundnut isolate, gbnv-type). comparative sequence analysis revealed that the genome organization of the s and m rna segments of both gbnv-mb and gbnv-type isolates was similar. however, considerable differences were observed in their intergenic regions (igrs) and the glycoprotein precurso ... | 2007 | 17385071 |
| nudiviruses and other large, double-stranded circular dna viruses of invertebrates: new insights on an old topic. | nudiviruses (nvs) are a highly diverse group of large, circular dsdna viruses pathogenic for invertebrates. they have rod-shaped and enveloped nucleocapsids, replicate in the nucleus of infected host cells, and possess interesting biological and molecular properties. the unassigned viral genus nudivirus has been proposed for classification of nudiviruses. currently, the nudiviruses comprise five different viruses: the palm rhinoceros beetle virus (oryctes rhinoceros nv, ornv), the hz-1 virus (he ... | 2009 | 19460388 |
| nss encoded by groundnut bud necrosis virus is a bifunctional enzyme. | groundnut bud necrosis virus (gbnv), a member of genus tospovirus in the family bunyaviridae, infects a large number of leguminosae and solanaceae plants in india. with a view to elucidate the function of nonstructural protein, nss encoded by the small rna genome (s rna), the nss protein of gbnv- tomato (karnataka) was over-expressed in e. coli and purified by ni-nta chromatography. the purified rnss protein exhibited an rna stimulated ntpase activity. further, this activity was metal ion depend ... | 2010 | 20305786 |
| genome organisation and sequence comparison suggest intraspecies incongruence in m rna of watermelon bud necrosis virus. | watermelon bud necrosis virus (wbnv), a member of the genus tospovirus, family bunyaviridae is an important viral pathogen in watermelon cultivation in india. the complete genome sequence properties of wbnv are not available. in the present study, the complete m rna sequence and the genome organisation of a wbnv isolate infecting watermelon in delhi (wbnv-wdel) were determined. the m rna was 4,794 nucleotides (nt) long and potentially coded for a movement protein (nsm) of 34.22 kda (307 amino ac ... | 2010 | 20480193 |
| determination of mhc binding peptides and epitopes from non-structural movement (nsm) protein of groundnut bud necrosis virus. | groundnut bud necrosis virus (gbnv) is recognized as one of the most economically important viruses and is known to affect several crops including peanut, potato, tomato and soybean. for managing plant virus diseases, determination of their causal agents' identity at an early stage of crop is a pre-requisite. in the present study, nsm protein of gbnv has been used to predict out mhc binding peptides and epitopes that are highly suitable for antigenicity. eighteen peptide regions were found to ha ... | 2010 | 20836757 |
| Investigations on the RNA binding and phosphorylation of groundnut bud necrosis virus nucleocapsid protein. | Groundnut bud necrosis virus belongs to the genus Tospovirus, infects a wide range of crop plants and causes severe losses. To understand the role of the nucleocapsid protein in the viral life cycle, the protein was overexpressed in E. coli and purified by Ni-NTA chromatography. The purified N protein was well folded and was predominantly alpha-helical. Deletion analysis revealed that the C-terminal unfolded region of the N protein was involved in RNA binding. Furthermore, the N protein could be ... | 2011 | 21947504 |
| Nucleocapsid protein gene mediated resistance against groundnut bud necrosis virus in tomato using sense and antisense constructs. | | 2011 | 21978164 |
| Interference in plant defense and development by non-structural protein NSs of Groundnut bud necrosis virus. | Groundnut bud necrosis virus (GBNV) infects a large number of leguminous and solanaceous plants. To elucidate the biological function of the non-structural protein encoded by the S RNA of GBNV (NSs), we studied its role in RNA silencing suppression and in viral pathogenesis. Our results demonstrated that GBNV NSs functions as a suppressor of RNA silencing using the agroinfiltration patch assay. An in silico analysis suggested the presence of pro-apoptotic protein Reaper-like sequences in the GBN ... | 2011 | 21924303 |
| production of cocktail of polyclonal antibodies using bacterial expressed recombinant protein for multiple virus detection. | cocktail of polyclonal antibodies (pab) were produced that will help in multiple virus detection and overcome the limitation of individual virus purification, protein expression and purification as well as immunization in multiple rabbits. a dual fusion construct was developed using conserved coat protein (cp) sequences of cucumber mosaic virus (cmv) and papaya ringspot virus (prsv) in an expression vector, pet-28a(+). the fusion protein (∼40kda) was expressed in escherichia coli and purified. l ... | 2014 | 24161814 |
| engineered single-chain variable fragment antibody for immunodiagnosis of groundnut bud necrosis virus infection. | few studies have been done on engineered antibodies for diagnosis of tospovirus infections. the present study was undertaken to develop a single-chain variable fragment (scfv) for specific diagnosis of infection by groundnut bud necrosis virus (gbnv), the most prevalent serogroup iv tospovirus in india. heavy chain (372 nucleotide [nt]) and light chain (363 nt) variable region clones obtained from a hybridoma were used to make an scfv construct that expressed a ~29-kda protein in e. coli. the sc ... | 2015 | 25698103 |
| groundnut bud necrosis virus encoded nsm associates with membranes via its c-terminal domain. | groundnut bud necrosis virus (gbnv) is a tripartite ambisense rna plant virus that belongs to serogroup iv of tospovirus genus. non-structural protein-m (nsm), which functions as movement protein in tospoviruses, is encoded by the m rna. in this communication, we demonstrate that despite the absence of any putative transmembrane domain, gbnv nsm associates with membranes when expressed in e. coli as well as in n. benthamiana. incubation of refolded nsm with liposomes ranging in size from 200-250 ... | 2014 | 24919116 |
| a nano-au/c-mwcnt based label free amperometric immunosensor for the detection of capsicum chlorosis virus in bell pepper. | accurate and on time diagnosis of plant viruses is an essential prerequisite for efficient control in field conditions. a number of diagnostic methods have been reported with the required level of sensitivity. here, we propose a label free immunosensor for efficient and sensitive detection of capsicum chlorosis virus (cacv) in bell pepper. antigen was immobilized over the surface of gold nanoparticle/multi-walled carbon nanotube (nano-au/c-mwcnt) screen printed electrodes using 1-ethyl-3-(3-dime ... | 2017 | 28293725 |
| molecular characterization of tospoviruses associated with ringspot disease in bell pepper from different districts of himachal pradesh. | bell pepper (capsicum annuum l.), an important cash crop for the farmers of himachal pradesh was found to be affected with tospovirus like disease. an extensive survey was conducted in the bell pepper grown areas in the five districts of himachal pradesh to identify and characterize the causative agent. hence, 60 symptomatic bell pepper plants exhibiting characteristics symptoms were collected from solan, sirmaur, hamirpur, kangra and bilaspur districts. out of 60 samples, 53 samples were found ... | 2016 | 27366771 |
| a novel viral rna helicase with an independent translation enhancement activity. | rna helicases have not been identified among negative sense rna viruses. in this study, it is shown that nonstructural protein (nss) of groundnut bud necrosis virus (gbnv) acts as a mg(2+) - and atp-dependent bipolar rna helicase. biophysical and biochemical analysis of the deletion mutants (nδ124 nss, cδ80 nss) revealed that both the n- and c-terminal residues are required for substrate binding, oligomerization and helicase activity, but are dispensable for atpase activity. interestingly, nss c ... | 2016 | 27001161 |
| development of interspecific solanum lycopersicum and screening for tospovirus resistance. | tospovirus has emerged as a serious viral pathogen for several crops including tomato. the tomato production is being severely affected worldwide by tospovirus. some reports have been published about the association of plant virus and development of human disease either by direct or indirect consumption. resistance to this virus has been identified as good source in wild tomato species (lycopersicum peruvianum). but the introgression of resistance genes into cultivated tomato lines and the devel ... | 2015 | 26587001 |
| complete genome sequence of mulberry vein banding associated virus, a new tospovirus infecting mulberry. | mulberry vein banding associated virus (mvbav) that infects mulberry plants with typical vein banding symptoms had been identified as a tentative species of the genus tospovirus based on the homology of n gene sequence to those of tospoviruses. in this study, the complete sequence of the tripartite rna genome of mvbav was determined and analyzed. the l rna has 8905 nucleotides (nt) and encodes the putative rna-dependent rna polymerase (rdrp) of 2877 aa amino acids (aa) in the viral complementary ... | 2015 | 26291718 |
| gbnv encoded movement protein (nsm) remodels er network via c-terminal coiled coil domain. | plant viruses exploit the host machinery for targeting the viral genome-movement protein complex to plasmodesmata (pd). the mechanism by which the non-structural protein m (nsm) of groundnut bud necrosis virus (gbnv) is targeted to pd was investigated using agrobacterium mediated transient expression of nsm and its fusion proteins in nicotiana benthamiana. gfp:nsm formed punctuate structures that colocalized with mcherry:plasmodesmata localized protein 1a (pdlp 1a) confirming that gbnv nsm local ... | 2015 | 25863878 |
| demonstration of helicase activity in the nonstructural protein, nss, of the negative-sense rna virus, groundnut bud necrosis virus. | the nonstructural protein nss, encoded by the s rna of groundnut bud necrosis virus (gbnv) (genus tospovirus, family bunyaviridae) has earlier been shown to possess nucleic-acid-stimulated ntpase and 5' α phosphatase activity. atp hydrolysis is an essential function of a true helicase. therefore, nss was tested for dna helicase activity. the results demonstrated that gbnv nss possesses bidirectional dna helicase activity. an alanine mutation in the walker a motif (k189a rnss) decreased dna helic ... | 2015 | 25643815 |
| influence of groundnut bud necrosis virus on the life history traits and feeding preference of its vector, thrips palmi. | the effect of groundnut bud necrosis virus (gbnv) infection on the life history traits of its vector, thrips palmi, and its feeding preference on gbnv-infected plants were studied. a significant difference was observed in the developmental period (first instar to adult) between the gbnv-infected and healthy thrips, wherein the developmental period of gbnv-infected thrips was decreased. however, there was no effect on the other parameters such as preadult mortality, adult longevity, and fecundity ... | 2017 | 28597727 |
| induction of cell death by tospoviral protein nss and the motif critical for cell death does not control rna silencing suppression activity. | groundnut bud necrosis virus induces necrotic symptoms in different hosts. previous studies showed reactive oxygen species-mediated programmed cell death (pcd) resulted in necrotic symptoms. transgenic expression of viral protein nss mimics viral symptoms. here, we showed a role for nss in influencing oxidative burst in the cell, by analyzing h2o2 accumulation, activities of antioxidant enzymes and expression levels of vacuolar processing enzymes, h2o2-responsive microrna 319a.2 plus its possibl ... | 2017 | 28527340 |