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development and stability of insecticide resistance in the leafminer liriomyza trifolii (diptera: agromyzidae) to cyromazine, abamectin, and spinosad.three populations of the leafminer, liriomyza trifolii (burgess), were collected from commercial ornamental production greenhouses in the united states and tested for susceptibility to three commercial insecticides. a leaf dip bioassay of leaves containing young (1-2-d-old) larvae was used. based on larval mortality and compared with a susceptible laboratory reference colony, the three strains varied in spectrum and level of resistance to the insecticides. ca-1, collected from gerbera daisy, was ...200414998134
effects of two plant extracts on larval leafminer liriomyza trifolii (diptera: agromyzidae) in tomatoes.aqueous extracts from two plants, urginea maritima l. (liliaceae) and euphorbia myrsinites l. (euphorbiaceae), were tested for their insecticidal activity against the leafminer liriomyza trifolii (burgess) on infested tomato, lycopersicon esculentum mill., plants in the laboratory and field. two grams of plant material was extracted with 100 ml of water and then diluted 1:100, 1:50, and 1:25 with distilled water. diluted plant extract was either applied to the infested tomato leaves or by soil d ...200415568346
the first finding of a rickettsia bacterium associated with parthenogenesis induction among insects.the larval endoparasitoid, neochrysocharis formosa (westwood), is an important natural enemy of the leafminer liriomyza trifolii (burgess) in japan. the thelytokous strain of n. formosa mostly produces female progeny. male progeny were produced by females treated with tetracycline, suggesting that microorganisms induce thelytoky in n. formosa. the result of nucleotide sequencing of the 16s rrna gene indicated that the parasitoid is infected with a rickettsia bacterium, which appears to be causat ...200616450063
juvenile and sublethal effects of selected pesticides on the leafminer parasitoids hemiptarsenus varicornis and diglyphus isaea (hymenoptera: eulophidae) from australia.the pest leafminers liriomyza huidobrensis (blanchard), liriomyza sativae (blanchard), and liriomyza trifolii (burgess) (diptera: agromyzidae) have spread into south east asia and oceania, and they are likely to reach australia in the near future. two translaminar pesticides, cyromazine and abamectin, currently provide effective chemical control of these pests, but because parasitoids can play an important role in controlling and preventing leafminer outbreaks, understanding the impact of pestic ...200516539101
improving the biological control of leafminers (diptera: agromyzidae) using the sterile insect technique.the leafminer liriomyza trifolii (burgess) (diptera: agromyzidae) is a worldwide pest of ornamental and vegetable crops. the most promising nonchemical approach for controlling liriomyza leafminers in greenhouses is regular releases of the parasitoid diglyphus isaea (walker) (hymenoptera: eulophidae). in the current study, we examine the hypothesis that the use of d. isaea for biological control of leafminers in greenhouse crops may be more practical and efficient when supplemented with addition ...200616937669
distinct roles of jasmonates and aldehydes in plant-defense responses.many inducible plant-defense responses are activated by jasmonates (jas), c(6)-aldehydes, and their corresponding derivatives, produced by the two main competing branches of the oxylipin pathway, the allene oxide synthase (aos) and hydroperoxide lyase (hpl) branches, respectively. in addition to competition for substrates, these branch-pathway-derived metabolites have substantial overlap in regulation of gene expression. past experiments to define the role of c(6)-aldehydes in plant defense resp ...200818382679
abamectin, pymetrozine and azadirachtin sequence as a unique solution to control the leafminer liriomyza trifolii (burgess) (diptera: agromyzidae) infesting garden beans (phaseolus vulgaris l.) in egypt.field trails were conducted to determine the performance of three different sequences as a unique solution for the control of the leaf miner liriomyza trifolii (burgess) (diptera: agromyzidae) infesting garden beans (phaseolus vulgaris l.) during the two successive seasons of 2004 and 2005. furthermore, during the evaluation period, the side effect against the ectoparasite diglyphus isaea (walker) (hymenoptera: eulophidae) was put into consideration. meanwhile, the comparative evaluation of the ...200718399491
feeding, oviposition and survival of liriomyza trifolii (diptera: agromyzidae) on bt and non-bt cottons.the effects of bt transgenic cottons (bt-i expressing cry1ac and bt-ii expressing cry1ab and cry2ab or cry1ab and cry1fa) and non-bt cottons on feeding, oviposition and longevity of adults, and development and survival of liriomyza trifolii larvae were studied under laboratory conditions; and infestation on four bt and two non-bt cotton traits were investigated under field conditions. laboratory choice and no-choice tests showed that l. trifolii adults were capable of distinguishing between bt c ...200918840315
permanent genetic resources added to molecular ecology resources database 1 february 2013-31 march 2013.this article documents the addition of 142 microsatellite marker loci to the molecular ecology resources database. loci were developed for the following species: agriophyllum squarrosum, amazilia cyanocephala, batillaria attramentaria, fungal strain ctey1 (ascomycota), gadopsis marmoratus, juniperus phoenicea subsp. turbinata, liriomyza sativae, lupinus polyphyllus, metschnikowia reukaufii, puccinia striiformis and xylocopa grisescens. these loci were cross-tested on the following species: amazi ...201323693143
action of some micronutrients on the infestation and yield components of faba bean by the aphid, aphis craccivora koch (aphididae, homoptera) and the leaf miner, liriomyza trifolii (burgess) (agromyzidae, diptera).field experiments were carried out in the two growing seasons of 1999/2000 and 2000/2001 on faba bean (vicia faba) plants in the experimental farm of agriculture research station at nubaria region, alexandria, which is considered as a newly reclaimed calcareous soil. the present investigation aimed to evaluate the effect of spraying faba bean plants with certain micronutrients, i.e. iron, manganese and zinc either in single double or triple combinations on the infestation by the aphid, aphis cra ...200415759427
use of monoclonal antibodies in an elisa for detecting an invasive pest insect, liriomyza trifolii (diptera: agromyzidae).a monoclonal antibody was prepared by the hybridoma technology. it reacted only with the protein of liriomyza trifolii (burgess) and not with that of chromatomyia horticola goureau or liriomyza sativae blanchard in indirect enzyme-linked immunosorbent assay. it was effective even after being diluted more than 8.192×10(6)-fold. the detection sensitivity of the antibody was 31.3 µg/ml under controlled conditions. positive reaction was achieved with all laboratory-reared l. trifolii samples, includ ...201526470159
host plant resistance and linear furanocoumarin content of apium accessions.linear furanocoumarin contents and antibiotic resistance to liriomyza trifolii (burgess) were documented for apium species being investigated in a celery breeding program. in no-choice tests, l. trifolii fed more, produced more offspring, and had the highest pupal and adult productivity on the widely planted cultivar 'tall utah' 52-70r (apium graveolens l.). antibiotic effects of the commercial cultivar 'tall utah' 52-70 hk and university of california families 87a-147 and 87a-338, derived from ...19902345223
disarming the jasmonate-dependent plant defense makes nonhost arabidopsis plants accessible to the american serpentine leafminer.here, we analyzed the interaction between arabidopsis (arabidopsis thaliana) and the american serpentine leafminer (liriomyza trifolii), an important and intractable herbivore of many cultivated plants. we examined the role of the immunity-related plant hormone jasmonate (ja) in the plant response and resistance to leafminer feeding to determine whether ja affects host suitability for leafminers. the expression of marker genes for the ja-dependent plant defense was induced by leafminer feeding o ...201324022267
effects of host sex, plant species, and putative host species on the prevalence of wolbachia in natural populations of bemisia tabaci (hemiptera: aleyrodidae): a modified nested pcr study.bemisia tabaci (gennadius) (hemiptera: aleyrodidae) is a globally distributed pest. one of the key endosymbionts in b. tabaci is wolbachia, an α-proteobacterium implicated in many important biological processes. previous studies indicated that the infection frequency of wolbachia in middle east-asia minor 1 (meam1) and mediterranean (med) varied greatly among populations in different areas. however, little is known about the factors that influence the prevalence of wolbachia in b. tabaci. in thi ...201526470122
insect resistance in tomato accessions and their hybrid derivatives in tamil nadu, india.host plant resistance offers a viable alternative to the use of chemical insecticides for managing insects infesting tomato. hence, a study was carried out in tamil nadu, india during 1996 to 2004. an exhaustive germplasm comprising 321 tomato accessions including cultivars, wild lines, land races, tribal/native tomatoes was gathered from various sources and screened for resistance against the major pest namely fruit worm, helicoverpa armigera hubner (noctuidae: lepidoptera). in the field screen ...200516628895
phytosanitary irradiation of liriomyza trifolii (diptera: agromyzidae).agromyzid leafminers are economic and quarantine pests of a variety of vegetables, flowers, and ornamental foliage. methyl bromide fumigation is often used as a phytosanitary treatment when quarantined agromyzids are found in shipped commodities; alternative treatments are sought. ionizing radiation is a viable alternative that is increasing in use worldwide. a dose of 400 gy is accepted by usda-aphis for all insects (except lepidoptera pupae and adults) on all commodities. efforts to lower this ...201122299344
induction of resistance against the leafminer, liriomyza trifolii, by jasmonic acid in sweet pepper.sweet pepper (capsicum annuum) leaves at the mature stage have strong ovipositional deterrence against liriomyza trifolii (burgess) (diptera, agromyzidae), whereas the cotyledons are fiercely attacked by the fly. treatment of the cotyledons with 50 microm and 100 microm of a jasmonic acid (ja) solution caused the plant to acquire strong oviposition deterrence against the leafminer. an hplc analysis of the ja-treated cotyledons revealed the inducible accumulation of a compound. based on spectrosc ...200717587684
nitrogenous ovipositional deterrents in the leaves of sweet pepper (capsicum annuum) at the mature stage against the leafminer, liriomyza trifolii (burgess).mature leaves of the sweet pepper, capsicum annuum, exhibited resistance against the american serpentine leafminer, liriomyza trifolii (burgess), agromyzidae. based on bioassay-guided fractionation, three compounds, namely 4-aminobutanoic acid, (2s,4r)-4-hydroxy-1-methyl-2-pyrrolidine carboxylic acid and 4-amino-1-beta-d-ribofuranosyl-2(1h)-pyrimidinone, were isolated from the leaves of sweet pepper. these compounds had significant oviposition deterrence towards adult flies of l. trifolii from l ...200717284863
ovipositional deterrent on mature stage of sweet pepper, capsicum annuum, against liriomyza trifolii (burgess).liriomyza trifolii (burgess), the american serpentine leafminer fly, is a well-known serious pest in the world. this insect species attacks plants of more than 21 families including solanaceae plants. a sweet pepper, capsicum annuum (solanaceae), on mature stage, however, shows resistance to this leafminer fly. this resistance is based on the ovipositional deterrent in the sweet pepper leaf against the fly species. based on the bioassay guided fractionation, phytol [(2e)-3,7,11,15-tetramethyl-2- ...200916320617
ovipositional deterrent in the sweet pepper, capsicum annuum, at the mature stage against liriomyza trifolii (burgess).liriomyza trifolii (burgess), the american serpentine leafminer fly, is well known as a serious pest throughout the world. this insect attack over 21 different plant families including solanaceae plants. the mature sweet pepper, capsicum annuum (solanaceae), however, shows resistance to this leafminer fly. this resistance is based on the ovipositional deterrent in the sweet pepper leaf against the fly species. based on bioassay-guided fractionation, luteolin 7-o-beta-d-apiofuranosyl-(1-->2)-beta ...200516244431
the leafminer liriomyza trifolii (diptera: agromyzidae) encapsulates its koinobiont parasitoid halticoptera circulus (hymenoptera: pteromalidae): implications for biological control.the koinobiont parasitoid halticoptera circulus (walker) is a potential biological control agent of leafminers, but it has only rarely been collected from the invasive leafminer, liriomyza trifolii (burgess), in japan. to understand why this is the case, parasitism and development of h. circulus in l. trifolii was compared with parasitism and development in two indigenous leafminer species, liriomyza chinensis kato and chromatomyia horticola (goureau). there was no significant difference in para ...201626639841
primer design for identifying economically important liriomyza species (diptera: agromyzidae) by multiplex pcr.leafminer flies, especially, liriomyza huidobrensis, liriomyza sativae and liriomyza trifolii, are quarantine species in many countries. their morphological similarity makes identification difficult. to develop a rapid, reliable, sensitive and simple molecular identification method using multiplex pcr, we newly sequenced the mitochondrial cytochrome oxidase i (coi) genes of liriomyza bryoniae, liriomyza chinensis, l. huidobrensis, l. sativae, l. trifolii, chromatomyia horticola and four parasito ...201323039969
polymerase chain reaction-restriction fragment length polymorphism assays to distinguish liriomyza huidobrensis (diptera: agromyzidae) from associated species on lettuce cropping systems in italy.the pea leafminer, liriomyza huidobrensis (blanchard) (diptera: agromyzidae), is a serious insect pest infesting open field lettuce plantings in northern italy. in these cropping systems, it coexists with several other agromyzid species that have negligible economic importance on open field vegetables. the rapid detection of l. huidobrensis is crucial for effective management strategies, but the identification of agromyzids to species can be very difficult at adult as well at immature stages. in ...200616937681
the complete mitochondrial genome of the leafminer liriomyza trifolii (diptera: agromyzidae).liriomyza trifolii (diptera: agromyzidae) is one of the most economically significant pests in the world. in this paper we present sequence data for the complete mitochondrial genome of l. trifolii. the circular genome is 16,141 bp long and contains one encoding region including 37 genes and one non-coding a+t-rich region. gene numbers and organization is similar to that of the typical insect mitochondrial genomes except that two additional trna genes are found in the a+t-rich region (trna(thr) ...201120376704
efficiency of trichome-based plant defense in phaseolus vulgaris depends on insect behavior, plant ontogeny, and structure.plant trichomes often function as physical barriers in preventing arthropod feeding and oviposition. even though insects are frequently reported being entrapped and killed by trichome traps, the actual trapping behavior has not yet been described in detail. capture experiments showed that capture efficiency during the plant's vegetative stage was considerably higher than in the fruiting and cotyledon stages. the ventral surface of the leaf was more effective in trapping flies than other parts of ...201729225609
niche comparison among two invasive leafminer species and their parasitoid opius biroi: implications for competitive displacement.fundamental to competitive displacement in biological invasion is that exotic species occupy the ecological niches of native species in novel environments. contrasting outcomes of competitive displacement have occurred between liriomyza trifolii and l. sativae in different geographical regions following their introduction. various factors have been advanced in an attempt to explain these different competitive outcomes, although none of these explanations have addressed the effects of niche diffe ...201728652592
species composition, distribution, and seasonal abundance of liriomyza leafminers (diptera: agromyzidae) under different vegetable production systems and agroecological zones in kenya.a longitudinal study to identify the species of liriomyza leafminer, their distribution, relative abundance, and seasonal variation, including their host range, was conducted in vegetable fields at three altitudes in kenya from november 2011 to november 2012. three main species were identified: liriomyza huidobrensis (blanchard), liriomyza sativae blanchard, and liriomyza trifolii (burgess), of which l. huidobrensis was the most abundant across all altitudes irrespective of the cropping season a ...201526313175
host plant-related parasitism and host feeding activities of diglyphus isaea (hymenoptera: eulophidae) on liriomyza huidobrensis, liriomyza sativae, and liriomyza trifolii (diptera: agromyzidae).host plant species can affect the behavior and attributes of parasitoids, such as host searching, oviposition, and offspring fitness. in this study, parasitism, host feeding, and sex ratios of diglyphus isaea (walker) (hymenoptera: eulophidae) on liriomyza huidobrensis (blanchard), liriomyza sativae blanchard, and liriomyza trifolii (burgess) (diptera: agromyzidae) larvae reared on phaseolus vulgaris l., pisum sativum l., solanum lycopersicum l., and vicia faba l. were determined. in no-choice t ...201222420268
cucurbitane glucosides from momordica charantia leaves as oviposition deterrents to the leafminer, liriomyza trifolii.the american serpentine leaf mining fly, liriomyza trifolii, whose larva feeds on more than 120 plant species is well characterized by its high degree of polyphagy. observations on the oviposition behavior by l. trifolii demonstrated that among cucurbitaceous plants, momordica charantia is rarely attacked by l. trifolii. the methanol extract of m. charantia leaves strongly deterred the females from ovipositing on kidney bean leaves treated at a concentration of 1 g leaf equivalent extract/ml. an ...200916610222
analysis of three leafminers' complete mitochondrial genomes.liriomyza trifolii (burgess), liriomyza huidobrensis (blanchard), and liriomyza bryoniae (kaltenbach), are three closely related and economically important leafminer pests in the world. this study examined the complete mitochondrial genomes of l. trifolii, l. huidobrensis and l. bryoniae, which were 16,141 bp, 16,236 bp and 16,183 bp in length, respectively. all of them displayed 37 typical animal mitochondrial genes and an a+t-rich region. the genomes were highly compact with only 60-68 bp of n ...201323954222
differential effects of pesticide applications on liriomyza huidobrensis (diptera: agromyzidae) and its parasitoids on pea in central kenya.three liriomyza species [liriomyza huidobrensis (blanchard), liriomyza trifolii (burgess), and liriomyza sativae blanchard] have been reported as the most important leafminer pests in vegetable production systems in africa. in kenya, farmers rely on indiscriminate synthetic insecticides use. on-farm field investigations were set up at three different locations (sagana, kabaru, and naromoru) in central kenya to determine the effect of pesticide application on the abundance of leafminers and their ...201526470177
the resolution of californian populations of liriomyza huidobrensis and liriomyza trifolii (diptera: agromyzidae) using pcr.liriomyza trifolii (burgess) and liriomyza huidobrensis (blanchard) are important vegetable pests in california. populations of each species differ in their impact in central and southern regions. this difference may be explained by geographical or host plant differences in each of the regions. we used random amplified polymorphic dna polymerase chain reactions to assess genetic differences between two laboratory populations of each species collected from central and southern california. individ ...200010971691
insecticide-mediated apparent displacement between two invasive species of leafminer fly.closely related invasive species may often displace one another, but it is often difficult to determine mechanisms because of the historical nature of these events. the leafmining flies liriomyza sativae and liriomyza trifolii have become serious invasive agricultural pests throughout the world. where both species have invaded the same region, one predominates over the other. although l. sativae invaded hainan island of china first, it recently has been displaced by the newly invasive l. trifoli ...201222662122
the complete mitochondrial genome of the leafminer liriomyza sativae (diptera: agromyzidae): great difference in the a+t-rich region compared to liriomyza trifolii.the complete mitochondrial genome sequence of liriomyza sativae blanchard (15,551bp) was determined and analyzed in this study. the circular genome contained 37 genes including 13 protein-coding genes, 22 trna genes, 2 rrna genes and an a+t-rich region. the initiation codons of coi and nd1 were 'atca' and 'gtg', respectively. nd2 gene used the truncated termination codon 't'. all the trna genes had the typical cloverleaf secondary structures except for trna(ser(agn)) gene, which was found with t ...201121703334
selection and validation of reference genes for quantitative real-time pcr analysis under different experimental conditions in the leafminer liriomyza trifolii (diptera: agromyzidae).liriomyza trifolii is a highly-invasive leafmining insect that causes significant damage to vegetables and horticultural crops worldwide. relatively few studies have quantified gene expression in l. trifolii using real-time quantitative pcr (rt-qpcr), which is a reliable and sensitive technique for measuring gene expression. rt-qpcr requires the selection of reference genes to normalize gene expression data and control for internal differences between samples. in this study, nine housekeeping ge ...201728746411
cloning and expression of genes encoding heat shock proteins in liriomyza trifolii and comparison with two congener leafminer species.the polyphagous agromyzid fly, liriomyza trifolii, is a significant and important insect pest of ornamental and vegetable crops worldwide. the adaptation of insects to different environments is facilitated by heat shock proteins (hsps), which play an important role in acclimation to thermal stress. in this study, we cloned and characterized five hsp-encoding genes of l. trifolii (lthsp20, lthsp40, lthsp60, lthsp70, and lthsp90) and monitored their expression under different thermal stresses usin ...201728727798
a decade of leafminer invasion in china: lessons learned.liriomyza trifolii (burgess) is a highly invasive species that has become established in agricultural and ornamental crops throughout the world. l. trifolii was first recorded in china in 2005 in guangdong province. subsequently, its known distribution in china has rapidly expanded to another 11 provinces (zhejiang, jiangsu, shanghai, fujian, guangxi, shandong, hainan, hebei, henan, hubei and anhui), where it is currently causing considerable damage and economic losses. experimental research and ...201728422455
development of a mechanical sexing system to improve the efficacy of an area-wide sterile insect release programme to control american serpentine leafminer (diptera: agromyzidae) in canadian ornamental greenhouses.american serpentine leafminer (asl), liriomyza trifolii (burgess), is a significant pest of greenhouse ornamental crops, and females damage leaf tissue with their ovipositor during feeding and oviposition. the sterile insect technique has been advocated as a non-chemical alternative to currently available control methods. in area-wide sterile insect release programmes, males act as true vectors of sterility. females should be eliminated from a cohort of pupae prior to irradiation to maximise pro ...201727888603
pesticide compatibility with natural enemies for pest management in greenhouse gerbera daisies.pesticides commonly used in commercial greenhouse management were evaluated for compatibility with two biological control agents: a leafminer parasitoid (diglyphus isaea [walker]), and a predatory mite (neoseiulus californicus [mcgregor]). these natural enemies were exposed to miticides, fungicides, and insecticides targeting leafminers, thrips, and whiteflies, according to label directions in laboratory vial assays, after which mortality at 12, 24, and 48 h was recorded. greater mortality of pr ...201324020270
trichomes and hydrocarbons associated with the tomato plant antixenosis to the leafminer.the objective of this work was to study the density of trichomes and hydrocarbons associated with the resistance by antixenosis of 42 subsamples of tomato plants from the horticultural germplasm bank of the universidade federal de viçosa (hgb-ufv) to liriomyza trifolii. these subsamples were studied in addition to 'santa clara' cultivar, which was used as a standard of susceptibility to leafminers. the evaluated characteristics were the number of mined leaflets per plant, mines per plant, tricho ...201222441610
impact of insecticides on parasitoids of the leafminer, liriomyza trifolii, in pepper in south texas.liriomyza leafminers (diptera: agromyzidae) are cosmopolitan, polyphagous pests of horticultural plants and many are resistant to insecticides. producers in south texas rely on insecticides as the primary management tool for leafminers, and several compounds are available. the objective of this study is to address the efficacy of these compounds for controlling liriomyza while minimizing their effects against natural enemies. research plots were established at texas agrilife research center at w ...201121864155
putative polymerase chain reaction markers for insecticide resistance in the leafminer liriomyza trifolii (diptera: agromyzidae) to cyromazine and abamectin.in this study, random amplified polymorphic dna polymerase chain reaction (rapd-pcr) was used to identify polymorphic genomic dna that would discriminate among cyromazine-resistant, abamectin-resistant, and susceptible liriomyza trifolii (burgess) (diptera: agromyzidae) leafminers. using a reference strain that was susceptible to both cyromazine and abamectin, and a cyromazine-resistant strain and an abamectin-resistant strain, 400 oligonucleotides were assayed using rapd-pcr. we found that two ...201021309244
a new oviposition deterrent to the leafminer, liriomyza trifolii: cucurbitane glucoside from momordica charantia.a new cucurbitane glucoside, 23-o-beta-d-glucopyranosyl-7-hydroxy-3-o-malonylcucurbita-5,24-dien-19-al, named momordicine v, has been isolated from momordica charantia leaves, along with the previously reported compounds, momordicines i, ii, iv and 3-o-malonylmomordicine i. the structure of the new compound was established on the basis of spectral analysis, as well as by its conversion to momordicine ii by alkaline catalyzed hydrolysis. momordicine v deterred significantly the oviposition by l. ...200917913080
cucurbitane triterpenoid oviposition deterrent from momordica charantia to the leafminer, liriomyza trifolii.females of liriomyza trifolii, a polyphagous leafminer, are often deterred from ovipositing on momordica charantia leaves. the methanol extract of m. charantia leaves showed strong oviposition deterrent activity against l. trifolii females on the host plant leaf when it was dipped in the methanol extract at a concentration of 1 g of fresh leaf equivalent/ml. bioguided fractionation of the methanol extract of the leaves of m. charantia led to the isolation of a new cucurbitane triterpenoid, 7,23- ...200516195588
multiple insect resistance in transgenic tomato plants over-expressing two families of plant proteinase inhibitors.protease inhibitors have been proposed as potential defense molecules for increased insect resistance in crop plants. compensatory over-production of insensitive proteases in the insect, however, has limited suitability of these proteins in plant protection, with very high levels of inhibitor required for increased plant resistance. in this study we have examined whether combined used of two inhibitors is effective to prevent this compensatory response. we show that leaf-specific over-expression ...200515821877
application of molecular techniques to distinguish liriomyza trifolii from l. sativae (diptera: agromyzidae) on tomato cultivation in japan.a molecular method is applied for differentiating the morphologically related leafminers liriomyza trifolli and l. sativae on tomato cultivation. the method requires multiplex polymerase chain reaction (pcr) amplification of a region of mitochondrial cytochrome oxidase dna using multiprimer sets. the method divides the mitochondrial fragment of l. trifolli into two fragments and l. sativae into three fragments. it is faster, less costly, and easier than random amplified polymorphic dna-pcr, pcr- ...200415279279
ecological history and evolution in a novel environment: habitat heterogeneity and insect adaptation to a new host plant.environmental heterogeneity has often been implicated in the maintenance of genetic variation. however, previous research has not considered how environmental heterogeneity might affect the rate of adaptation to a novel environment. in this study, i used an insect-plant system to test the hypothesis that heterogeneous environments maintain more genetic variation in fitness components in a novel environment than do uniform environments. to manipulate recent ecological history, replicate populatio ...199728568810
the effect of leaf-mining by liriomyza trifolii on seed set in greenhouse marigolds.the effect of foliage feeding by the serpentine leafminer, liriomyza trifolii (burgess), on seed production and germination by male-sterile marigolds (tagetes erecta l.) was examined over two cropping seasons in commercial production greenhouses. five components of t. erecta relative fitness (the number of flowers, ovules, and germinations per plant, seed mass, and plant height) were compared in plants grown under four different control strategies representing three different intensities of l. t ...199227759209
effect of leaf nitrogen content of tomato plants on preference and performance of a leafmining fly.in a study on intraspecific host plant acceptability, liriomyza trifolii females that had previously been exposed to plants of high nitrogen content, showed a feeding and oviposition preference for plants of high nitrogen (minkenberg and fredrix 1989). females showed a preference to feed and oviposit on the high middle leaves within plants. it was hypothesized that the preference between plants was related to a better performance of females and offspring on high nitrogen plants compared to low n ...199028312998
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