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candida phangngensis sp. nov., an anamorphic yeast species in the yarrowia clade, isolated from water in mangrove forests in phang-nga province, thailand.two yeast strains (tm2-16 and pt1-17(t)) were isolated by membrane filtration from samples of estuarine water collected from two mangrove forests, in khao lumpee-haad thaimueang national park and mu ko ra-ko prathong national park, phang-nga province, thailand. analysis of the d1/d2 domain of the large-subunit rdna sequences revealed that the sequences of the two strains were identical. the closest species in terms of pairwise sequence similarity was candida galli, but the level of nucleotide su ...200818218960
candida galli strain pgo6: a novel isolated yeast strain capable of transformation of isoeugenol into vanillin and vanillic acid.candida galli strain pgo6 isolated from oil-contaminated water is the first isolated yeast strain which is capable to form vanillin and vanillic acid during isoeugenol biotransformation. the products were confirmed by thin-layer chromatography (tlc), changes in the uv absorption pattern and high-performance liquid chromatography (hplc). the phenotypic and physiochemical characteristics as well as molecular phylogenetic analysis based on amplification the its1-5.8s-its2 rdna regions indicated the ...201021086130
mitochondrial genomes of yeasts of the yarrowia clade.candida alimentaria, candida deformans, candida galli, and candida phangngensis have been recently reported to be the close relatives of yarrowia lipolytica. to explore this clade of yeasts, we sequenced the mitochondrial genome (mtdna) of these four species and compared it with the mtdna of y. lipolytica. the five mtdnas exhibit a similar architecture and a high level of similarity of protein coding sequences. genome sizes are variable, ranging from 28 017 bp in c. phangngensis to 48 508 bp in ...201122188421
yeasts occurring in surface and mouth cavity of two chelonian species, podocnemis expansa schweigger and p. unifilis troschel (reptilia: chelonia: pelomedusidae), in the javaés river border of araguaia national park in brazil.thirty-eight specimens of free-ranging podocnemis expansa (amazon turtle) and 22 of p. unifilis (tracajá) were screened for yeast isolation from surface (plastron, skin, and nails), eye, and mouth cavity. a hundred and eighteen yeast isolates belonging to 39 species were obtained. debaryomyces hansenii, candida galli, c. sake, and rhodotorula mucilaginosa were the most frequent species isolated from these chelonians. species diversity measured by shannon's index was shown to be low and a degree ...201020936145
polyphasic re-examination of yarrowia lipolytica strains and the description of three novel candida species: candida oslonensis sp. nov., candida alimentaria sp. nov. and candida hollandica sp. nov.the type strain of yarrowia lipolytica and 38 strains identified as yarrowia lipolytica, four strains of candida deformans, including the type and two subcultures of the type, two strains of candida galli and six unidentified strains that resembled y. lipolytica were examined by pcr fingerprints using primers m13 and (gac)5. the same strains, together with four strains of the recently introduced candida yakushimensis nom. inval., were sequenced for the d1/d2 domain of the 26s rrna gene and parts ...200717911320
candida galli sp. nov., a new yeast from poultry.six strains of an unknown yeast species, phenotypically resembling yarrowia lipolytica and isolated from chicken breast and chicken liver, were studied. the investigation of their small (18s) and large (26s) subunit rdna revealed a robust genetic difference between these strains and the type strain of y. lipolytica. a consistent difference in the physiological properties, suitable for separation of the two taxa, was also found. the description of the new anamorphic yeast species, candida galli i ...200415280644
candida galli as a cause of tinea unguium-molecular characterization of a rare clinical fungal entity.we present the first clinical report of an infection caused by candida galli, an anamorphic yeast species in the yarrowia clade. c. galli has been described in the literature only four times, but never before it has been isolated from clinical samples. the colony morphology on sabouraud medium and morphotype on chromagar candida medium were similar to c. lipolytica as well as the carbon assimilation profile. the phenotypic differences with c. lipolytica were the non-assimilation of n-acetyl gluc ...201425037486
identification of three yeast species using the conventional and internal transcribed spacer region sequencing methods as first or second global record from human superficial infections.during the mycological analysis of skin and nail samples taken from patients with onychomycosis and tineas in assiut city, it is interesting to report that yeast fungi were the main causal agents being cultured from 45.79% of total cases. in general, 21 species of yeast were isolated. some of these are reported for the first time from clinical specimens. from the literature available up-to-date around the world, this study reports for the first time saccharomycopsis fibuligera as the causal agen ...201627392537
heterologous expression of lipases ylip4, ylip5, ylip7, ylip13 and ylip15 from yarrowia lipolytica msr80 in e. coli: substrate specificity, kinetic comparison and enantioselectivity.five lipase genes, ylip4, ylip5, ylip7, ylip13 and ylip15, from yarrowia lipolytica msr80 were cloned and expressed in pezz18-hb101 system. the lipases shared maximum sequence identity with candida galli lipase, while they shared structural similarity with ylip2 of y. lipolytica clib122. the enzymes, purified using igg sepharose, had specific activities in the range of 7-25 u/mg. biochemical characteristics of all the lipases varied with respect to thermostability, substrate specificity and enan ...201627775845
relative incidence of ascomycetous yeasts in arctic coastal environments.previous studies of fungi in polar environments have revealed a prevalence of basidiomycetous yeasts in soil and in subglacial environments of polythermal glaciers. ascomycetous yeasts have rarely been reported from extremely cold natural environments, even though they are known contaminants of frozen foods. using media with low water activity, we have isolated various yeast species from the subglacial ice of four glaciers from the coastal arctic environment of kongsfjorden, spitzbergen, includi ...201121221569
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