| complementation of the host range restriction of southern cowpea mosaic virus in bean by southern bean mosaic virus. | vigna unguiculata (cowpea) and phaseolus vulgaris (common bean) are permissive hosts for southern cowpea mosaic virus (scpmv) and southern bean mosaic virus (sbmv), respectively. neither of these two sobemoviruses systemically infects the permissive host of the other. although bean cells are permissive for scpmv rna synthesis, they do not support the assembly of this virus. thus, the host range restriction of scpmv in bean may occur at the level of movement and may involve the inability of scpmv ... | 2000 | 10612668 |
| structure of native and expanded sobemoviruses by electron cryo-microscopy and image reconstruction. | rice yellow mottle virus (rymv) and southern bean mosaic virus, cowpea strain (scpmv) are members of the sobemovirus genus of rna-containing viruses. we used electron cryo-microscopy (cryo-em) and icosahedral image analysis to examine the native structures of these two viruses at 25 a resolution. both viruses have a single tightly packed capsid layer with 180 subunits assembled on a t=3 icosahedral lattice. distinctive crown-like pentamers emanate from the 12 5-fold axes of symmetry. the exterio ... | 2000 | 11023786 |
| complete nucleotide sequence of sesbania mosaic virus: a new virus species of the genus sobemovirus. | the complete nucleotide sequence of the sesbania mosaic virus (semv) genomic rna was determined by sequencing overlapping cdna clones. the semv genome is 4149 nucleotides in length and encodes four potential overlapping open reading frames (orfs). comparison of the nucleotide sequence and the deduced amino acid sequence of the four orfs of semv with that of other sobemoviruses revealed that semv was closest to southern bean mosaic virus arkansas isolate (sbmv-ark, 73% identity). the 5' non-codin ... | 2001 | 11315633 |
| in vitro analysis of an rna binding site within the n-terminal 30 amino acids of the southern cowpea mosaic virus coat protein. | southern cowpea mosaic virus (scpmv) is a positive-sense rna virus with t = 3 icosahedral symmetry. the coat protein (cp) has two domains, the random (r) domain and the shell (s) domain. the r domain is formed by the n-terminal 64 amino acids (aa) and is localized to the interior of the particle where it is expected to interact with the viral rna. the r domain (aa 1--57) was expressed in escherichia coli as a recombinant protein (rwtr) containing a nonviral c-terminal extension with two histidin ... | 2001 | 11485399 |
| membrane activity of the southern cowpea mosaic virus coat protein: the role of basic amino acids, helix-forming potential, and lipid composition. | southern cowpea mosaic virus (scpmv) is a spherical rna virus with t = 3 icosahedral symmetry. the particle is composed of 180 subunits of the coat protein (cp) and one copy of the positive-sense viral rna. the cp has two domains, the random (r) domain formed by the n-terminal 64 aa and the shell (s) domain (aa 65--260). the r domain is highly charged, with 11 of the n-terminal 30 residues being basic. it is localized to the interior of the native particle where it may interact with the viral rn ... | 2001 | 11878899 |
| sobemoviruses possess a common cfmv-like genomic organization. | based on structural differences in the orf2 region, the sobemoviruses have been subdivided into southern cowpea mosaic virus (scpmv)-like and cocksfoot mottle virus (cfmv)-like types of genome organization. however, nearly identical amino acid sequences are encoded by these subgroups in different reading frames of orf2, suggesting that insertion or deletion of appropriate nucleotides could restore similar genomic organizations for these viruses. we resequenced the regions of inconsistency for is ... | 2007 | 17115301 |
| metagenomic-based screening and molecular characterization of cowpea-infecting viruses in burkina faso. | cowpea, (vigna unguiculata l. (walp)) is an annual tropical grain legume. often referred to as "poor man's meat", cowpea is one of the most important subsistence legumes cultivated in west africa due to the high protein content of its seeds. however, african cowpea production can be seriously constrained by viral diseases that reduce yields. while twelve cowpea-infecting viruses have been reported from africa, only three of these have so-far been reported from burkina faso. here we use a virion- ... | 2016 | 27764211 |