| transfer of bifidobacterium inopinatum and bifidobacterium denticolens to scardovia inopinata gen. nov., comb. nov., and parascardovia denticolens gen. nov., comb. nov., respectively. | bifidobacterium inopinatum crociani et al. 1996 and bifidobacterium denticolens crociani et al. 1996 have distinct phenotypic characteristics and low g+c contents compared with other bifidobacteria. in the 16s rrna phylogenetic tree, these two species grouped in an independent subcluster. in our previous work, partial heat-shock protein 60 (hsp60) gene-sequence analysis also indicated that these two species had distinct taxonomic positions. in this work, the complete hsp60 genes of five represen ... | 2002 | 12054242 |
| occurrence of the family bifidobacteriaceae in human dental caries and plaque. | despite the constant presence of members of bifidobacteriaceae in the human oral cavity, few studies of their occurrence in this habitat have been made. the presence and the distribution in human plaque and dental caries of bifidobacterium dentium, scardovia inopinata and parascardovia denticolens, all belonging to the family bifidobacteriaceae, were studied. the identification to species level was based on morphological and growth type features, fermentation tests, polyacrylamide gel electropho ... | 2006 | 16707878 |
| alloscardovia omnicolens gen. nov., sp. nov., from human clinical samples. | the taxonomic position of 12 isolates tentatively assigned to the genus bifidobacterium on the basis of a limited phenotypic characterization was examined. the isolates were collected between 1978 and 2005 in belgium, sweden and norway, and originated from various human clinical samples, including urine, blood, urethra, oral cavity, tonsil, and abscesses of lung and aortic valve. on the basis of band number and clustering analysis, repetitive dna element-based pcr fingerprinting using the boxa1r ... | 2007 | 17625172 |
| isolation and identification of bifidobacteriaceae from human saliva. | bifidobacteriaceae were isolated from saliva and infected dentine by using a mupirocin-based selective medium. of the saliva samples, 94% harbored bifids. the mean concentration (+/- the standard error) was 4.46 (+/-0.12) log(10)(cfu per ml + 1), and the predominant isolates were bifidobacterium dentium, b. longum, scardovia inopinata, parascardovia denticolens, and alloscardovia omnicolens. | 2008 | 18723652 |
| association between bifidobacteriaceae and the clinical severity of root caries lesions. | the isolation of members of the family bifidobacteriaceae (bifids) from oral samples has been sporadic and a recent cloning study has suggested that they are not detectable in root caries lesions. | 2009 | 19121067 |
| the isolation of bifidobacteria from occlusal carious lesions in children and adults. | the aim of this study was to enumerate and identify bifidobacteria from occlusal carious lesions in permanent and deciduous teeth. samples of infected dentine were obtained from 24 active occlusal lesions in deciduous teeth and from 15 occlusal lesions in permanent teeth. plaque samples from sound occlusal surfaces of 12 caries-free adults and 12 children were also obtained. the bifidobacterial strains were isolated in mupirocin-containing selective media, gram-stained and subcultured for identi ... | 2009 | 19494490 |
| bifidobacterium bombi sp. nov., from the bumblebee digestive tract. | gram-positive-staining, anaerobic, non-spore-forming, lactate- and acetate-producing bacterial strains were isolated from the digestive tracts of different bumblebee species (bombus lucorum, bombus pascuorum and bombus lapidarius). all of the isolates produced fructose-6-phosphate phosphoketolase activity. a representative strain, bluci/tpt, was characterized further. cells of strain bluci/tpt showed occasional bifurcation and irregular constrictions. the bacterium utilized a wide range of carbo ... | 2009 | 19567560 |
| scardovia wiggsiae sp. nov., isolated from the human oral cavity and clinical material, and emended descriptions of the genus scardovia and scardovia inopinata. | six strains of anaerobic, pleomorphic gram-positive bacilli, isolated from the human oral cavity and an infected arm wound, were subjected to a comprehensive range of phenotypic and genotypic tests and were found to comprise a homogeneous group. 16s rrna gene sequence analysis revealed that the isolates were most closely related to scardovia inopinata ccug 35729(t) (94.8-94.9 % 16s rrna gene sequence similarity). the isolates were saccharolytic and produced acetic and lactic acids as end product ... | 2011 | 20139283 |
| cultivable anaerobic microbiota of severe early childhood caries. | severe early childhood caries (ecc), while strongly associated with streptococcus mutans using selective detection (culture, pcr), has also been associated with a widely diverse microbiota using molecular cloning approaches. the aim of this study was to evaluate the microbiota of severe ecc using anaerobic culture. the microbial composition of dental plaque from 42 severe ecc children was compared with that of 40 caries-free children. bacterial samples were cultured anaerobically on blood and ac ... | 2011 | 21289150 |
| microbiota of severe early childhood caries before and after therapy. | severe early childhood caries (ecc) is difficult to treat successfully. this study aimed to characterize the microbiota of severe ecc and evaluate whether baseline or follow-up microbiotas are associated with new lesions post-treatment. plaque samples from 2- to 6-year-old children were analyzed by a 16s rrna-based microarray and by pcr for selected taxa. severe-ecc children were monitored for 12 months post-therapy. by microarray, species associated with severe-ecc (n = 53) compared with caries ... | 2011 | 21868693 |