| clinical relevance of the recently described species aeromonas aquariorum. | twenty-two human extraintestinal isolates (11 from blood) and three isolates recovered from patients with diarrhea were genetically characterized as aeromonas aquariorum, a novel species known only from ornamental fish. the isolates proved to bear a considerable number of virulence genes, and all were resistant to amoxicillin (amoxicilline), cephalothin (cefalotin), and cefoxitin. biochemical differentiation from the most relevant clinical species is provided. | 2009 | 19741075 |
| aeromonas aquariorum sp. nov., isolated from aquaria of ornamental fish. | during a survey to determine the prevalence of aeromonas strains in water and skin of imported ornamental fish, 48 strains presumptively identified as aeromonas were isolated but they could not be identified as members of any previously described aeromonas species. these strains were subjected to a polyphasic approach including phylogenetic analysis derived from gyrb, rpod and 16s rrna gene sequencing, dna-dna hybridization, maldi-tof ms analysis, genotyping by rapd and extensive biochemical and ... | 2008 | 18450708 |
| phylogenetic analysis and identification of aeromonas species based on sequencing of the cpn60 universal target. | an analysis of the universal target (ut) sequence from the cpn60 gene was performed in order to evaluate its usefulness in phylogenetic and taxonomic studies and as an identification marker for the genus aeromonas. sequences of 555 bp, corresponding to the ut region, were obtained from a collection of 35 strains representing all of the species and subspecies of aeromonas. from the analysis of these sequences, a range of divergence of 0-23.3% was obtained, with a mean of 11.2+/-0.9%. comparative ... | 2009 | 19567585 |
| phylogenetic evidence suggests that strains of aeromonas hydrophila subsp. dhakensis belong to the species aeromonas aquariorum sp. nov. | three strains of aeromonas hydrophila subspecies dhakensis, including the type strain, were subjected to phylogenetic analysis by sequencing gyrb, rpod, and 16s rrna genes and compared with all known aeromonas species. the obtained gyrb and rpod phylogenetic trees clearly suggested that these a. hydrophila subsp. dhakensis strains indeed belong to the species a. aquariorum. this finding may indicate that, at the time of "dhakensis" subspecies description, the strains were incorrectly identified ... | 2009 | 18839248 |
| characterization of a new strain of aeromonas dhakensis isolated from diseased pacu fish (piaractus mesopotamicus) in brazil. | this study provides a detailed description and characterization of a strain of aeromonas dhakensis isolated from a diseased juvenile piaractus mesopotamicus obtained from the fish farm of the national center for continental fish research and conservation (cepta/icmbio), in the state of são paul, brazil. biochemical tests using the vitek 2 automated bacterial identification system identified the isolate to genus level; however, further molecular analysis of the 16s rrna, gyrb and rpod genes showe ... | 2016 | 26850370 |
| aeromonas hydrophila subsp. dhakensis subsp. nov., isolated from children with diarrhoea in bangladesh, and extended description of aeromonas hydrophila subsp. hydrophila (chester 1901) stanier 1943 (approved lists 1980). | a group of 10 aeromonas hydrophila-like strains referred to as group bd-2, which mainly comprised haemolytic and cytotoxic isolates from diarrhoeal children in bangladesh, was subjected to a polyphasic taxonomic study including fluorescent amplified fragment length polymorphism (faflp) and eric-pcr fingerprinting, microplate dna-dna hybridization and extensive phenotypic characterization. comparison of faflp and eric-pcr genotypic profiles indicated that the group bd-2 isolates constituted a sep ... | 2002 | 12054229 |
| a disease model of muscle necrosis caused by aeromonas dhakensis infection in caenorhabditis elegans. | a variety of bacterial infections cause muscle necrosis in humans. caenorhabditis elegans has epidermis and bands of muscle that resemble soft-tissue structures in mammals and humans. here, we developed a muscle necrosis model caused by aeromonas dhakensis infection in c. elegans. our data showed that a. dhakensis infected and killed c. elegans rapidly. characteristic muscle damage in c. elegans induced by a. dhakensis was demonstrated in vivo. relative expression levels of host necrosis-associa ... | 2016 | 28101079 |
| genotypic and phenotypic identification of aeromonas species and cpha-mediated carbapenem resistance in queensland, australia. | infection caused by aeromonas spp. ranges from superficial wound infection to life-threatening septicemia. carbapenem resistance due to metallo-beta-lactamase, cpha encoded by the cpha gene, is a significant problem. this study defines aeromonas spp. causing clinical disease in queensland, australia. phenotypic tests for carbapenemase detection were assessed. one hundred aeromonas isolates from blood (22), wound (46), sterile sites (11), stool (18), eye (2), and sputum (1) were characterized by ... | 2016 | 26971634 |
| distribution and phenotypic and genotypic detection of a metallo-β-lactamase, cpha, among bacteraemic aeromonas isolates. | the objectives of the study were to investigate the distribution of cpha-related genes (cpha) encoding a cpha metallo-β-lactamase (mbl) among 51 consecutive aeromonas blood isolates and to compare different phenotypic methods for detecting cpha. the presence of cpha was detected by pcr. four phenotypic methods, the imipenem-edta combined disc test, imipenem-edta mbl etest, agar dilution test and modified hodge test (mht), were used to detect imipenem susceptibility and mbl production. the result ... | 2012 | 22322339 |
| molecular characterization of clinical isolates of aeromonas species from malaysia. | aeromonas species are common inhabitants of aquatic environments giving rise to infections in both fish and humans. identification of aeromonads to the species level is problematic and complex due to their phenotypic and genotypic heterogeneity. | 2012 | 22383958 |
| genome sequence of a novel human pathogen, aeromonas aquariorum. | aeromonas aquariorum, a recently described species, is associated with a variety of human diseases. we present here the first genome sequence of a. aquariorum strain aak1, which was isolated as the sole pathogen from the blood of a patient with septicemia and necrotizing fasciitis. | 2012 | 22815437 |
| aeromonas aquariorum septicemia and enterocolitis in a cirrhotic patient. | aeromonas aquariorum is a recently described aeromonas species. we present a cirrhotic patient with septicemia and bloody diarrhea in whom a. aquariorum was isolated from the blood and stool. the species identification was based on sequence analysis of the partial rpob and rpod genes. the a. aquariorum isolates carried genes encoding hemolysin, aerolysin, and the cytotonic enterotoxin alt. significant a. aquariorum-induced cytotoxicity in intestinal epithelial cells was also demonstrated. | 2012 | 22995364 |
| identification and virulence of aeromonas dhakensis, pseudomonas mosselii and microbacterium paraoxydans isolated from nile tilapia, oreochromis niloticus, cultivated in mexico. | to identify bacterial pathogens of diseased niie tilapia oreochromis niloticus and determine their virulence. | 2013 | 23758410 |
| aeromonas aquariorum clinical isolates: antimicrobial profiles, plasmids and genetic determinants. | the objective of this study was to investigate the antimicrobial resistance patterns of 47 clinical isolates of aeromonas aquariorum and to identify the presence of plasmids and the relevant antibiotic resistance genes (args). antibiotic susceptibilities were determined by the standard disc diffusion method. the presence of plasmids and args was detected by gel electrophoresis and monoplex pcr. resistance to amoxicillin/clavulanic acid (98%), amoxicillin (91%), gentamicin (13%), trimethoprim/sul ... | 2013 | 23312608 |
| identification error of aeromonas aquariorum: a causative agent of septicemia. | a genetic reidentification of 7 aeromonas blood isolates revealed that phenotype-based identification systems misidentified 5 (71.4%) isolates. in aeromonas strains, a. aquariorum was the most common misidentified organism and showed the most potent cytotoxic activities against human blood cell lines, suggesting that the correct identification of a. aquariorum is important. | 2013 | 23461831 |
| reclassification of aeromonas hydrophila subsp. dhakensis huys et al. 2002 and aeromonas aquariorum martínez-murcia et al. 2008 as aeromonas dhakensis sp. nov. comb nov. and emendation of the species aeromonas hydrophila. | previous studies indicate that aeromonas aquariorum and aeromonas hydrophila subsp. dhakensis are the same taxon and suggest that they should be synonymized. using a polyphasic approach, the phenotypic and phylogenetic relationship of a. aquariorum with the 3 defined a. hydrophila subspecies (i.e. dhakensis, hydrophila, ranae) was investigated. phylogenetic trees derived from the 16s rrna, rpod or gyrb genes and a multilocus phylogenetic analysis (with the concatenated sequences of gyrb, rpod, r ... | 2013 | 23485124 |
| molecular characterization of aeromonas species isolated from farmed eels (anguilla japonica). | seventy aeromonas strains were identified by phylogenetic analysis using housekeeping genes (gyrb and rpod) in order to investigate etiological agents for aeromoniasis in farmed eels (anguilla japonica). the phylogenetic analysis showed that aeromonas aquariorum (n=22, 31.4%) was the predominant species among the investigated eel strains, followed by aeromonas caviae (n=16, 22.9%), a. veronii (n=13, 18.6%), a. hydrophila (n=12, 17.1%), a. jandaei (n=4, 5.7%), a. media (n=2, 2.9%), and a. trota ( ... | 2013 | 23499189 |
| the improved pcr of the fsta (ferric siderophore receptor) gene differentiates the fish pathogen aeromonas salmonicida from other aeromonas species. | the members of the genus aeromonas are autochthonous of aquatic ecosystems and several species have been associated to septicaemia, ulcerative and haemorrhagic diseases in fish, causing significant mortality in both wild and farmed, freshwater and marine fish species. the species aeromonas salmonicida is generally recognized as the most important fish pathogen responsible for epidemic outbreaks of furunculosis in salmonids, also being able to produce infections in other cultured fish such as tur ... | 2013 | 23890674 |
| aqu-1, a chromosomal class c β-lactamase, among clinical aeromonas dhakensis isolates: distribution and clinical significance. | aeromonas dhakensis, a recently described aeromonas sp. formerly called aeromonas aquariorum, is associated with human infections. in this study, a chromosomal gene, blaaqu-1, was identified in a. dhakensis aak1 that constitutes a 1143-bp open reading frame and is 87% identical to the gene encoding ceph in aeromonas hydrophila. an escherichia coli top10 cell transformant harbouring blaaqu-1 was resistant to cefotaxime but not to cefepime. mrna expression of blaaqu-1 in the cefotaxime-resistant m ... | 2013 | 24055254 |
| severe sepsis due to aeromonas aquariorum in a patient with liver cirrhosis. | thus far, aeromonas aquariorum infection has been unrecorded in korea. herein, we report a fatal case of a. aquariorum infection in a 77-year-old male patient with liver cirrhosis. the bacterium isolated from a blood culture was initially mistaken as aeromonas hydrophila using the vitek2 identification system. in spite of intravenous ceftriaxone therapy, the patient was exacerbated by multiple organ dysfunction. by 4 days after admission, there was no hope for treatment or remission of symptoms ... | 2013 | 24270141 |
| prospecting metal-resistant plant-growth promoting rhizobacteria for rhizoremediation of metal contaminated estuaries using spartina densiflora. | in the salt marshes of the joint estuary of tinto and odiel rivers (sw spain), one of the most polluted areas by heavy metals in the world, spartina densiflora grows on sediments with high concentrations of heavy metals. furthermore, this species has shown to be useful for phytoremediation. the total bacterial population of the rhizosphere of s. densiflora grown in two estuaries with different levels of metal contamination was analyzed by pcr denaturing gradient gel electrophoresis. results sugg ... | 2014 | 24281681 |
| distribution, virulence-associated genes and antimicrobial resistance of aeromonas isolates from diarrheal patients and water, china. | to determine the prevalence of aeromonas infections in diarrheal patients, the distribution of virulence-associated genes and antibiotic resistance among different aeromonas species in china. | 2015 | 25447712 |
| occurrence, molecular characterization, and antimicrobial susceptibility of aeromonas spp. in marine species of shrimps cultured at inland low salinity ponds. | we aimed to document the risk of aeromonas spp. in marine shrimp species cultured in inland low salinity ponds in thailand. in 14 of 18 shrimp samples retrieved from inland grow-up ponds, aeromonas spp. were detected at ranges from 4667 to 1,500,000 cfu/g body weight. the phylogenetic tree constructed with the gyrb and cpn60 concatenated sequences indicated that the 87 isolates consisted of aeromonas veronii (70%), aeromonas aquariorum (18%), aeromonas caviae (7%), aeromonas jandaei (2%), and ae ... | 2015 | 25583334 |
| classification of aeromonas spp. isolated from water and clinical sources and distribution of virulence genes. | in this work, 84 isolates of aeromonads were isolated from water and clinical samples, identified, and characterized. identification was based on routine phenotyping combined with multiplex pcr. in this study, multiplex pcr was retested and reevaluated and its identification key was enhanced by 17 newly described species and five subspecies. identification score increased from 36 % (only phenotyping) to 90 % when supported with multiplex pcr. further description of isolates included detection of ... | 2016 | 27416863 |
| isolation and characterization of acyl homoserine lactone-producing bacteria during an urban river biofilm formation. | the presence and diversity of acyl homoserine lactone (ahl)-producers in an urban river biofilm were investigated during 60-day biofilm formation. ahl biosensors detected the presence of ahl-producers in 1-60-day river biofilms. screening for ahl-producers resulted in 17 aeromonas spp., 3 pseudomonas spp., 3 ensifer spp., and 1 acinetobacter sp. among these isolates, six of them were closely related to acinetobacter tjernbergiae, aeromonas allosaccharophila, aeromonas aquariorum, aeromonas janda ... | 2012 | 23090571 |
| re-identification of aeromonas isolates from chironomid egg masses as the potential pathogenic bacteria aeromonas aquariorum. | egg masses of the non-biting midge chironomous sp. have recently been found to serve as a reservoir for vibrio cholerae and aeromonas species. these insects are widely distributed in freshwater and evidence suggests that they may disseminate pathogenic bacteria species into drinking water systems. in the current study the taxonomy of 26 aeromonas isolates, previously recovered from chironomid egg masses, was re-evaluated. it was found that 23 isolates, which had previously been identified as aer ... | 2011 | 23761256 |
| aeromonas aquariorum is widely distributed in clinical and environmental specimens and can be misidentified as aeromonas hydrophila. | genotypic characterization of 215 aeromonas strains (143 clinical, 52 environmental, and 20 reference strains) showed that aeromonas aquariorum (60 strains, 30.4%) was the most frequently isolated species in clinical and water samples and could be misidentified as aeromonas hydrophila by phenotypic methods. | 2011 | 21697316 |
| validation of a partial rpob gene sequence as a tool for phylogenetic identification of aeromonads isolated from environmental sources. | a collection of 50 aeromonads isolated from environmental sources were studied, together with all known aeromonas nomenspecies, by phenotypic, amplified 16s rdna restriction analysis (16s rdna rflp) and by partial sequence alignment of both 16s rdna and rpob genes. although most of the type strain showed a unique phenotypic pattern, a database constructed on type strain phenotype allowed the identification of only 24% of the isolates. analysis of 16s rdna rflp and the rpob sequence were almost c ... | 2010 | 20453908 |
| aeromonas dhakensis, an increasingly recognized human pathogen. | aeromonas dhakensis was first isolated from children with diarrhea in dhaka, bangladesh and described in 2002. in the past decade, increasing evidence indicate this species is widely distributed in the environment and can cause a variety of infections both in human and animals, especially in coastal areas. a. dhakensis is often misidentified as a. hydrophila, a. veronii, or a. caviae by commercial phenotypic tests in the clinical laboratory. correct identification relies on molecular methods. in ... | 2016 | 27303382 |
| draft genome sequence of aeromonas dhakensis strain f2s2-1, isolated from the skin surface of an indian oil sardine (sardinella longiceps). | draft genome sequencing of aeromonas dhakensis strain f2s2-1, isolated from the skin surface of an indian oil sardine (sardinella longiceps), has been carried out. the draft genome was roughly 4.7 mb in size with 61.7% g+c content. annotation of the genome yielded 4,337 genes coding for proteins, trnas, and rrnas. annotation also revealed the presence of 52 genes linked to resistance to antibiotics/toxic compounds. pathway analysis revealed the presence of novobiocin biosynthetic genes and genes ... | 2016 | 27540048 |
| clinical implications of species identification in monomicrobial aeromonas bacteremia. | advances in aeromonas taxonomy have led to the reclassification of aeromonads. hereon, we aimed to re-evaluate the characteristics of aeromonas bacteremia, including those of a novel species, aeromonas dhakensis. | 2015 | 25679227 |
| high prevalence of blactx-m group genes in aeromonas dhakensis isolated from aquaculture fish species in south korea. | the prevalence of resistant genes against β-lactams in 119 aeromonas strains was determined. a large number (99.2%) of the present fish strains were resistant to one or more β- lactams including ceftiofur, amoxicillin-clavulanic acid, ampicillin, piperacillin and cefpodoxime. among antibiotic resistance phenotypes, the simultaneous resistance to all β-lactams occurred in 25.2% (n=30) of all strains, which consisted of 18 strains of a. dhakensis, 8 strains of a. caviae, 2 strains of a. hydrophila ... | 2014 | 25649940 |
| matrix-assisted laser desorption ionization-time of flight mass spectrometry can accurately differentiate aeromonas dhakensis from a. hydrophila, a. caviae, and a. veronii. | among 217 aeromonas isolates identified by sequencing analysis of their rpob genes, the accuracy rates of identification of a. dhakensis, a. hydrophila, a. veronii, and a. caviae were 96.7%, 90.0%, 96.7%, and 100.0%, respectively, by the cluster analysis of spectra generated by matrix-assisted laser desorption ionization-time of flight mass spectrometry. | 2014 | 24759711 |
| a comparative study of clinical aeromonas dhakensis and aeromonas hydrophila isolates in southern taiwan: a. dhakensis is more predominant and virulent. | aeromonas dhakensis, often phenotypically identified as aeromonas hydrophila, is an important human pathogen. the present study aimed to compare the clinical and biological features of a. dhakensis and a. hydrophila isolates from human wounds. a total of 80 aeromonas wound isolates collected between january 2004 and april 2011 were analysed. the species was identified by the dna sequence matching of rpod and gyrb (or rpob if necessary). most of the aeromonas isolates were identified as a. dhaken ... | 2014 | 24237662 |
| degradation of norgestrel by bacteria from activated sludge: comparison to progesterone. | natural and synthetic progestagens in the environment have become a concern due to their adverse effects on aquatic organisms. laboratory studies were performed to investigate aerobic biodegradation of norgestrel by bacteria from activated sludge in comparison with progesterone, and to identify their degradation products and biotransformation pathways. the degradation of norgestrel followed first order reaction kinetics (t1/2 = 12.5 d), while progesterone followed zero order reaction kinetics (t ... | 2013 | 23952780 |
| aeromonas hydrophila subsp. dhakensis--a causative agent of gastroenteritis imported into the czech republic. | out of the twenty-one a. hydrophila complex isolates obtained during a routine examination of human diarrhoeal faeces, two a. hydrophila subsp. dhakensis isolates (p1097 = ccm 7329 and p1165) were successfully identified by ribotyping. the correct taxonomic position of the a. hydrophila subsp. dhakensis ccm 7329 was verified by cpn60 sequencing (genebank accession number hm536193). the remaining a. hydrophila complex isolates were identified as a. hydrophila subsp. hydrophila. the ability of bio ... | 2012 | 23020031 |
| aeromonas hydrophila subsp. dhakensis isolated from feces, water and fish in mediterranean spain. | eight aeromonas hydrophila-like arabinose-negative isolates from diverse sources (i.e., river freshwater, cooling-system water pond, diseased wild european eels, and human stools) sampled in valencia (spain) during 2004-2005, were characterized by 16s rrna gene sequencing and extensive biochemical testing along with reference strains of most aeromonas species. these isolates and all reference strains of a. hydrophila subsp. dhakensis and a. aquariorum showed a 16s rrna sequence similarity of 99. ... | 2012 | 22472298 |
| the rapid induction of carbapenem-resistance in an aeromonas dhakensis blood isolate. | meropenem-susceptible and -resistant aeromonas dhakensis isolates from blood cultures of a fatal case of septicemia were analyzed. the two isolates were homologous and gene expression of metallo-β-lactamase in the resistant strain was upregulated. physicians should be aware of the possibility of the induction of carbapenem-resistance, following the use of carbapenems in the treatment of aeromonas infection. | 2016 | 26743140 |
| aeromonas dhakensis pneumonia and sepsis in a neonate risso's dolphin grampus griseus from the mediterranean sea. | a neonate risso's dolphin grampus griseus was found stranded alive on a beach in catalonia, spain. rehabilitation attempts were unsuccessful and it died 2 d later, showing pneumonia and sepsis. a pure bacterial culture was obtained from all tissues and blood and identified as aeromonas hydrophila using the api 20ne. however, sequencing the rpod gene showed that the strain in fact belongs to a. dhakensis, making this the first report of fatal haemorrhagic-necrotizing pneumonia and sepsis due to t ... | 2015 | 26378409 |
| characterization and gene expression analysis of pacu (piaractus mesopotamicus) inducible nitric oxide synthase (inos) following aeromonas dhakensis infection. | nitric oxide (no) is an important effector molecule which is involved in a myriad of biological processes, including immune responses against pathogens such as parasites, virus and bacteria. during the inflammatory processes in vertebrates, no is produced by the inducible nitric oxide synthase (inos) enzyme in practically all nucleated cells to suppress or kill intracellular pathogens. the aim of the present study was to characterize the full coding region of the inos gene of pacu (piaractus mes ... | 2017 | 29277697 |