| characterization of a new picorna-like virus, himetobi p virus, in planthoppers. | picorna-like virus particles, 29 nm in diameter, were purified from apparently healthy laodelphax striatellus fallen. the virus particles had a buoyant density of 1.352 g/ml in cscl and a sedimentation coefficient of 161 s. the virus capsid proteins consisted of three major polypeptides of m(r)s 36,500, 33,000 and 28,000, and three minor polypeptides. the virus contained a major ssrna of m(r) 2.8 x 10(6) and was also frequently associated with a minor dsrna of m(r) 4 x 10(6). the 3' end of the s ... | 1992 | 1634869 |
| genetic variation in the ability of a planthopper vector; laodelphax striatellus (fallén) to acquire the rice stripe virus. | | 1967 | 6025062 |
| electrophoretic karyotype of intracellular yeast-like symbiotes in rice planthoppers and anobiid beetles. | chromosomal dna molecules of intracellular yeast-like symbiotes (yls) of three species of rice planthoppers and two species of anobiid beetles have been separated by pulsed-field gel electrophoresis. probable chromosome numbers of nilaparvata lugens, sogatella furcifera, and laodelphax striatellus, were 4, 4, and 5, respectively, and tentative genome sizes were 17.3, 17.6, and 20.1 mbp, respectively, based upon migration of individual chromosome-sized dna relative to the size standards of schizo ... | 1995 | 7722339 |
| phylogenetic position of yeast-like symbiotes of rice planthoppers based on partial 18s rdna sequences. | the rice planthoppers, nilaparvata lugens, sogatella furcifera, and laodelphax striatellus, harbor intracellular yeast-like symbiotes (yls) in the fat body. the yls are obligated endosymbiotes and are transovarially inherited. nucleotide sequences of the 18s rrna genes from the yls were analyzed by direct sequencing. the yls of the three species of planthoppers were monophyletic and their taxonomic positions were in the class pyrenomycetes in the subphylum ascomycotina. | 1995 | 7787846 |
| nonpathogenic nilaparvata lugens reovirus is transmitted to the brown planthopper through rice plant. | nilaparvata lugens reovirus (nlrv) was found in a colony of the brown planthopper, n. lugens, an important plant virus vector. unlike with usual phytopathogenic reoviruses, there are no visible symptoms on a rice plant which has been attacked by nlrv-infected planthoppers. manner of transmission, host range, and multiplication of nlrv in rice plant were studied by enzyme-linked immunosorbent assay or polymerase chain reaction using reverse transcriptase. nlrv was transovumly transmitted to about ... | 1995 | 7871743 |
| pcr-based detection of wolbachia, cytoplasmic incompatibility microorganisms, infected in natural populations of laodelphax striatellus (homoptera: delphacidae) in central japan: has the distribution of wolbachia spread recently? | cytoplasmic incompatibility is caused in various insects by intracellular infection with rickettsia-like microorganisms of the genus wolbachia. in japan laodelphax striatellus shows unidirectional cytoplasmic incompatibility between northeastern and southwestern populations. in this study, nine natural populations of l. striatellus collected from central japan, including the geographic boundary between the two cytotype populations, were analysed for wolbachia infection by pcr using primers speci ... | 1995 | 8825761 |
| allozyme polymorphism and geographic variation in the small brown planthopper, laodelphax striatellus (homoptera: delphacidae). | the small brown planthopper, laodelphax striatellus, immigrates annually into japan over the east china sea from the asian mainland. it is not known whether this long-distance dispersal has any effect on the genetic structure of japanese l. striatellus populations. the dispersal of l. striatellus is suspected to be relevant to the population dynamics of infection with the parasitic bacterium wolbachia, which causes cytoplasmic incompatibility in l. striatellus. wolbachia infection has spread wit ... | 1997 | 9559095 |
| detection and assignment of proteins encoded by rice black streaked dwarf fijivirus s7, s8, s9 and s10. | the proteins encoded by rice black streaked dwarf fijivirus (rbsdv) genomic segments 7-10 (s7-s10) were characterized. open reading frames (orfs) from these segments were expressed as fusion proteins in escherichia coli. antibodies raised against the expressed products were used as probes to determine whether the viral orfs encode structural proteins. in western blots, antibodies to the expressed s8 and s10 products reacted with a core capsid (65 kda) and a major outer capsid (56 kda) protein, r ... | 1998 | 9634092 |
| determining the nucleotide sequence and capsid-coding region of himetobi p virus: a member of a novel group of rna viruses that infect insects. | we determined the complete genome sequence of himetobi p virus (hipv), an insect picorna-like virus, which was isolated from the small brown planthopper, laodelphax striatellus. the genome of hipv consists of 9,275 nucleotides excluding the poly (a) tail, and contains two large open reading frames (orfs), which were separated by a 176-nucleotide noncoding region. the deduced amino acid sequence of the first orf contains core motifs of picornaviral helicase, protease, and rna-dependent rna polyme ... | 1999 | 10550677 |
| [phylogenetic relationships and evolution of yeast-like symbionts of the small brown planthoppers based on partial 18s rdna sequences]. | the yeast-like symbionts are isolated and purified from the small brown planthoppers collected from five areas in china. the 18s rdna sequences of them are determined. combined with the sequences of other fungi, we have constructed a comprehensive phylogenetic tree. the results suggested that the taxonomic positions of the yls are in the class pyrenomycetes in the subphylum ascomycotina and the yls have the closest relationship with h. chrysospermus. the yls of different areas in china and japan ... | 2000 | 10887693 |
| wolbachia infection and cytoplasmic incompatibility in the cricket teleogryllus taiwanemma. | wolbachia are cytoplasmically inherited bacteria found in many arthropods. they induce various reproductive alterations in their hosts, including cytoplasmic incompatibility, thelytokous parthenogenesis, feminization and male-killing. in this study, we examined wolbachia infection and its effects on the host cricket teleogryllus taiwanemma. in a phylogenetic study based on the wsp gene coding for a wolbachia surface protein, the wolbachia strain harboured by t. taiwanemma was clustered together ... | 2000 | 10903165 |
| infection density of wolbachia and incompatibility level in two planthopper species, laodelphax striatellus and sogatella furcifera. | wolbachia, a bacterial endosymbiote of arthropods, causes cytoplasmic incompatibility (ci) in many insect species. ci traits were studied in two planthopper species, laodelphax striatellus and sogatella furcifera, and wolbachia densities in these planthopper species were calculated by quantitative pcr methods. the ci level of l. striatellus was quite high and even aged males strongly caused ci. in contrast, s. furcifera showed partial ci, and males lost their ability to cause ci with age. wolbac ... | 2001 | 11267910 |
| insect symbiosis: derivation of yeast-like endosymbionts within an entomopathogenic filamentous lineage. | yeast-like endosymbionts (ylss) of insects often are restricted to specific hosts and are essential to the host's survival. for example, in planthoppers (homoptera: delphacidae), endosymbionts function in sterol utilization and nitrogen recycling for the hosts. our study, designed to investigate evolutionary changes in the yls lineage involved in the planthopper association, strongly suggests an origin of the ylss from within the filamentous ascomycetes (euascomycetes), not the true yeasts (sacc ... | 2001 | 11371588 |
| wolbachia infection shared among planthoppers (homoptera: delphacidae) and their endoparasite (strepsiptera: elenchidae): a probable case of interspecies transmission. | wolbachia, a group of parasitic bacteria of arthropods, are believed to be horizontally transmitted among arthropod taxa. we present a new probable example of interspecies horizontal transmission of wolbachia by way of an endoparasite based on the conformity of wolbachia gene sequences. field samples of two rice planthoppers, laodelphax striatellus and sogatella furcifera possessed identical wolbachia. among three major endoparasites of planthoppers, a strepsipteran, elenchus japonicus, harboure ... | 2001 | 11555254 |
| [cytopathology of plants infected with viruses. ultrastructure of the leaf cells of cereals affected by rhabdoviruses]. | ultrathin sections of oat, wheat, and ryegrass leaves from healthy plants and plants infected with rhabdoviruses by leafhoppers laodelphax striatellus fallen were studied under the electron microscope. the bacilliform virions often surrounded by endoplasmic reticulum (er) membranes, viroplasm, and tubular structures conforming, in diameter and structure, to the rhabdovirion nucleocapsid were observed in the cytoplasm of leaf cells of the diseased plants. the cereal pseudorosette virus [(165-200) ... | 2002 | 12068435 |
| superinfection of laodelphax striatellus with wolbachia from drosophila simulans. | wolbachia are maternally inherited, intracellular alpha-proteobacteria that infect a wide range of arthropods. they manipulate the reproduction of hosts to facilitate their spread into host populations, through ways such as cytoplasmic incompatibility (ci), parthenogenesis, feminization and male killing. the influence of wolbachia infection on host populations has attracted considerable interest in their possible role in speciation and as a potential agent of biological control. in this study, w ... | 2003 | 12522428 |
| in vitro cultivation of wolbachia in insect and mammalian cell lines. | wolbachia infecting the small brown planthopper, laodelphax striatellus, were successfully maintained and cultivated in two insect and one mammalian cell lines. the bacteria with the planthopper ovary were introduced into the flasks with the cultures of the cell lines. the wolbachia proliferated in mosquito (aedes albopictus) and lepidopteran (heliothis zea) cell lines and in the mouse cell line, l929. proliferation of wolbachia was confirmed by electron microscopy and quantitative polymerase ch ... | 2002 | 12534342 |
| detecting rice stripe virus (rsv) in the small brown planthopper (laodelphax striatellus) with high specificity by rt-pcr. | rice stripe disease, caused by rice stripe virus (rsv), may lead to severe or even crippling losses in many rice-cultured countries and regions. as the most important vector of rsv, the small brown planthopper (sbph) (laodelphax striatellus) is largely responsible for the epidemic phase of the disease. therefore, a rapid identification of rsv in the sbph is of a great need for disease forecasting. a reverse transcription polymerase chain reaction (rt-pcr) assay is described to amplify a rsv gene ... | 2003 | 12951219 |
| [qtl analysis for rice stripe disease resistance gene using recombinant inbred lines (rils) derived from crossing of kinmaze and dv85]. | rice stripe disease transmitted by small brown planthopper (laodelphax striatellus fall.) is one of the most serious viral diseases in east asia. the disease is severely epidemic in most rice growing areas where the main cultivars are susceptible or moderately susceptible to rice stripe virus. in this research, a recombinant inbred lines (rils) population of 81 lines derived from a cross of kinmaze (japonica)/dv85(indica) by the single seed descent method was used to detect quantitative trait lo ... | 2004 | 15195569 |
| detection and localization of rice stripe virus gene products in vivo. | the genome of the tenuivirus, rice stripe virus (rsv) comprises four rnas, the smallest three of which each contain two open reading frames (orfs) arranged in an ambisense manner. the expression of the orfs from rnas 2-4 in plants and the insect vector, laodelphax striatellus, was studied using antisera raised against the gene products. in western blotting of the proteins from infected plants, the molecular masses of p2, p3, pc3 (nucleocapsid protein, n) and p4 (major non-structural protein, ncp ... | 2005 | 16025247 |
| phylogenetic position of the yeast-like symbiotes of tagosodes orizicolus (homoptera: delphacidae) based on 18s ribosomal dna partial sequences. | tagosodes orizicolus muir (homoptera: delphacidae), the endemic delphacid species of tropical america carries yeast-like symbiotes (yls) in the abdominal fat bodies and the ovarial tissues, like other rice planthoppers of asia. these yls are obligate symbiotes, which are transmitted transovarially, and maintain a mutualistic relationship with the insect host. this characteristic has made in vitro culture and classification of yls rather difficult using conventional methods. nevertheless, microor ... | 2004 | 17361570 |
| transmission of rice stripe virus acquired from frozen infected leaves by the small brown planthopper (laodelphax striatellus fallen). | rice stripe disease, caused by rice stripe virus (rsv), is one of the most serious rice diseases in temperate and subtropical regions of the world. since rsv is not transmissible mechanically, an insect transmission test was the original basis for identification of the viral population and cultivar resistance. a simple, rapid and reliable method is described by which virus-free small brown planthoppers acquired rsv from frozen infected rice leaves and transmitted the virus to healthy rice plants ... | 2007 | 17614147 |
| a one-step real time rt-pcr assay for quantifying rice stripe virus in rice and in the small brown planthopper (laodelphax striatellus fallen). | rice stripe virus (rsv) is an important pathogen affecting rice production in subtropical and temperate regions. one-step real time rt-pcr methods using the taqman probe are described for quantitative detection of rsv in rice tissues and in laodelphax striatellus fallen, the small brown planthopper (sbph). primers and probe for specific detection of rsv were designed within the conserved region identified within the coat protein (cp) gene sequence. a dna fragment was amplified for mimicking the ... | 2008 | 18586332 |
| transinfection of wolbachia in planthoppers: nymphal injection of cultured wolbachia and infection dynamics. | wolbachia species are intracellular symbionts that cause reproductive alterations in arthropods. transinfection experiments have been performed in many arthropod species to elucidate the interaction between wolbachia and a new host. to ease transinfection of this bacterium to new arthropod hosts, we introduced two techniques: nymphal injection instead of embryonic injection and the use of a cultured source of wolbachia instead of direct transfer from donors to recipients. wolbachia in the small ... | 2009 | 20021758 |
| massively parallel pyrosequencing-based transcriptome analyses of small brown planthopper (laodelphax striatellus), a vector insect transmitting rice stripe virus (rsv). | the small brown planthopper (laodelphax striatellus) is an important agricultural pest that not only damages rice plants by sap-sucking, but also acts as a vector that transmits rice stripe virus (rsv), which can cause even more serious yield loss. despite being a model organism for studying entomology, population biology, plant protection, molecular interactions among plants, viruses and insects, only a few genomic sequences are available for this species. to investigate its transcriptome and d ... | 2010 | 20462456 |
| expression of defense genes and activities of antioxidant enzymes in rice resistance to rice stripe virus and small brown planthopper. | the rice variety tai06-1 is resistant to rice stripe disease and xiushui63 is a highly susceptible rice variety to this disease. these two varieties were used to analyze the expression patterns of defense genes and antioxidant defense responses at the seedling stage, upon feeding with viruliferous small brown planthopper (sbph) and nonviruliferous sbph, respectively. the expression levels of cp (coat protein) gene of rice stripe virus (rsv) were higher upon feeding with viruliferous sbph in xius ... | 2011 | 21300551 |
| permanent genetic resources added to molecular ecology resources database 1 january 2009-30 april 2009. | this article documents the addition of 283 microsatellite marker loci to the molecular ecology resources database. loci were developed for the following species: agalinis acuta; ambrosia artemisiifolia; berula erecta; casuarius casuarius; cercospora zeae-maydis; chorthippus parallelus; conyza canadensis; cotesia sesamiae; epinephelus acanthistius; ficedula hypoleuca; grindelia hirsutula; guadua angustifolia; leucadendron rubrum; maritrema novaezealandensis; meretrix meretrix; nilaparvata lugens; ... | 2009 | 21564911 |
| genetic dissection of the resistance to rice stripe virus present in the indica rice cultivar 'ir24' | rice stripe disease, caused by rice stripe virus (rsv) and transmitted by the small brown planthopper (laodelphax striatellus fallen), is one of the most serious viral diseases of rice in temperate east asian production regions. prior quantitative trait loci (qtl) mapping has established that oryza sativa l. subsp. indica 'ir24' carries positive alleles at the three loci qstv3, qstv7, and qstv11-i. here, we report an advanced backcross analysis based on three selected chromosome segment substitu ... | 2011 | 21793697 |
| five proteins of laodelphax striatellus are potentially involved in the interactions between rice stripe virus and vector. | rice stripe virus (rsv) is the type member of the genus tenuivirus, which relies on the small brown planthopper (laodelphax striatellus fallén) for its transmission in a persistent, circulative-propagative manner. to be transmitted, virus must cross the midgut and salivary glands epithelial barriers in a transcytosis mechanism where vector receptors interact with virions, and as propagative virus, rsv need utilize host components to complete viral propagation in vector cells. at present, these m ... | 2011 | 22028913 |
| Identification of yeast associated with the planthopper, Perkinsiella saccharicida: potential applications for Fiji leaf gall control. | Yeasts associate with numerous insects, and they can assist the metabolic processes within their hosts. Two distinct yeasts were identified by PCR within the planthopper Perkinsiella saccharicida, the vector of Fiji disease virus to sugarcane. The utility of both microbes for potential paratransgenic approaches to control Fiji leaf gall (FLG) was assessed. Phylogenetic analysis showed one of the microbes is related to yeast-like symbionts from the planthoppers: Laodelphax striatellus, Nilaparvat ... | 2011 | 21850475 |
| suppression of ns3 and mp is important for the stable inheritance of rnai-mediated rice stripe virus (rsv) resistance obtained by targeting the fully complementary rsv-cp gene. | rice stripe virus (rsv) is a viral disease that seriously impacts rice production in east asia, most notably in korea, china, and japan. highly rsv-resistant transgenic japonica rice plants were generated using a dsrnai construct designed to silence the entire sequence region of the rsv-cp gene. transgenic rice plants were inoculated with a population of viruliferous insects, small brown planthoppers (sbph), and their resistance was evaluated using elisa and an infection rate assay. a correlatio ... | 2011 | 22134721 |
| wolbachia from the planthopper laodelphax striatellus establishes a robust, persistent, streptomycin-resistant infection in clonal mosquito cells. | the obligate intracellular bacterium, wolbachia pipientis (rickettsiales: anaplasmataceae), distorts reproduction of its arthropod hosts to facilitate invasion of naïve populations. this property makes wolbachia an attractive "gene drive" agent with potential applications in the control of insect vector populations. genetic manipulation of wolbachia will require in vitro systems for its propagation, genetic modification, amplification, and introduction into target insects. here we show that wolb ... | 2012 | 23271364 |
| the wolbachia wo bacteriophage proteome in the aedes albopictus c/wstr1 cell line: evidence for lytic activity? | wolbachia pipientis (rickettsiales), an obligate intracellular alphaproteobacterium in insects, manipulates host reproduction to maximize invasion of uninfected insect populations. modification of host population structure has potential applications for control of pest species, particularly if wolbachia can be maintained, manipulated, and genetically engineered in vitro. although wolbachia maintains an obligate mutualism with genome stability in nematodes, arthropods can be co-infected with dist ... | 2015 | 26427709 |
| depletion of host cell riboflavin reduces wolbachia levels in cultured mosquito cells. | wolbachia is an obligate intracellular alphaproteobacterium that occurs in arthropod and nematode hosts. wolbachia presumably provides a fitness benefit to its hosts, but the basis for its retention and spread in host populations remains unclear. wolbachia genomes retain biosynthetic pathways for some vitamins, and the possibility that these vitamins benefit host cells provides a potential means of selecting for wolbachia-infected cell lines. to explore whether riboflavin produced by wolbachia i ... | 2014 | 24789726 |
| proteomic analysis of interaction between a plant virus and its vector insect reveals new functions of hemipteran cuticular protein. | numerous viruses can be transmitted by their corresponding vector insects; however, the molecular mechanisms enabling virus transmission by vector insects have been poorly understood, especially the identity of vector components interacting with the virus. here, we used the yeast two-hybrid system to study proteomic interactions of a plant virus (rice stripe virus, rsv, genus tenuivirus) with its vector insect, small brown planthopper (laodelphax striatellus). sixty-six proteins of l. striatellu ... | 2015 | 26091699 |
| proteomic profiling of a robust wolbachia infection in an aedes albopictus mosquito cell line. | wolbachia pipientis, a widespread vertically transmitted intracellular bacterium, provides a tool for insect control through manipulation of host-microbe interactions. we report proteomic characterization of wstr, a wolbachia strain associated with a strong cytoplasmic incompatibility phenotype in its native host, laodelphax striatellus. in the aedes albopictus c/wstr1 mosquito cell line, wstr maintains a robust, persistent infection. ms/ms analyses of gel bands revealed a protein 'footprint' do ... | 2014 | 25155417 |
| small interfering rna pathway modulates initial viral infection in midgut epithelium of insect after ingestion of virus. | numerous viruses are transmitted in a persistent manner by insect vectors. persistent viruses establish their initial infection in the midgut epithelium, from where they disseminate to the midgut visceral muscles. although propagation of viruses in insect vectors can be controlled by the small interfering rna (sirna) antiviral pathway, whether the sirna pathway can control viral dissemination from the midgut epithelium is unknown. infection by a rice virus (southern rice black streaked dwarf vir ... | 2015 | 26537672 |
| identification and profiling of conserved and novel micrornas in laodelphax striatellus in response to rice black-streaked dwarf virus (rbsdv) infection. | micrornas (mirnas) are small non-coding endogenous rna molecules that play important roles in various biological processes. this study examined microrna profiles of laodelphax striatellus using the small rna libraries derived from virus free (vf) and rice black-streaked dwarf virus (rbsdv) infected (rb) insects. a total of 59 mature mirnas (46 mirna families) were identified as conserved insect mirnas in both vf and rb libraries. among these conserved mirnas, 24 were derived from the two arms of ... | 2014 | 26484150 |
| rice stripe virus affects the viability of its vector offspring by changing developmental gene expression in embryos. | plant viruses may affect the viability and development process of their herbivore vectors. small brown planthopper (sbph) is main vector of rice stripe virus (rsv), which causes serious rice stripe disease. here, we reported the effects of rsv on sbph offspring by crossing experiments between viruliferous and non-viruliferous strains. the life parameters of offspring from different cross combinations were compared. the hatchability of f1 progeny from viruliferous parents decreased significantly, ... | 2015 | 25601039 |
| facilitation of rice stripe virus accumulation in the insect vector by himetobi p virus vp1. | the small brown planthopper (sbph) is the main vector for rice stripe virus (rsv), which causes serious rice stripe disease in east asia. to characterize the virus-vector interactions, the sbph cdna library was screened with rsv ribonucleoprotein (rnp) as bait using a gal4-based yeast two-hybrid system. the interaction between rsv-rnp and the himetobi p virus (hipv, an insect picorna-like virus) vp1 protein was identified. the relationships between hipv and rsv in sbph were further investigated, ... | 2015 | 25807055 |
| diapause induction and termination in the small brown planthopper, laodelphax striatellus (hemiptera: delphacidae). | the small brown planthopper, laodelphax striatellus (fallén) enters the photoperiodic induction of diapause as 3rd or 4th instar nymphs. the photoperiodic response curves in this planthopper showed a typical long-day response type with a critical daylength of approximately 11 h at 25 °c, 12 h at 22 and 20 °c and 12.5 h at 18 °c, and diapause induction was almost abrogated at 28 °c. the third stage was the most sensitive stage to photoperiod. the photoperiodic response curve at 20 °c showed a gra ... | 2014 | 25188306 |
| overexpression of multiple detoxification genes in deltamethrin resistant laodelphax striatellus (hemiptera: delphacidae) in china. | the small brown planthopper (sbph), laodelphax striatellus (fallén), is one of the major rice pests in asia and has developed resistance to multiple classes of insecticides. understanding resistance mechanisms is essential to the management of this pest. biochemical and molecular assays were performed in this study to systematically characterize deltamethrin resistance mechanisms with laboratory-selected resistant and susceptible strains of sbph. | 2013 | 24324548 |
| transcriptome analysis of the small brown planthopper, laodelphax striatellus carrying rice stripe virus. | rice stripe virus (rsv), the type member of the genus tenuivirus, transmits by the feeding behavior of small brown planthopper (sbph), laodelphax striatellus. to investigate the interactions between the virus and vector insect, total rna was extracted from rsv-viruliferous sbph (rvls) and non-viruliferous sbph (nvls) adults to construct expressed sequence tag databases for comparative transcriptome analysis. over 30 million bases were sequenced by 454 pyrosequencing to construct 1,538 and 953 of ... | 0 | 25288960 |
| rna interference depletion of the halloween gene disembodied implies its potential application for management of planthopper sogatella furcifera and laodelphax striatellus. | sogatella furcifera and laodelphax striatellus are economically important rice pests in china by acting as vectors of several rice viruses, sucking the phloem sap and blocking the phloem vessels. ecdysteroid hormone 20-hydroxyecdysone regulates insect development and reproduction. a cytochrome p450 monooxygenase cyp302a1 (22-hydroxylase), encoded by the halloween gene disembodied (dib), plays a critical role in ecdysteroidogenesis. the objective of this study is to test whether dib genes are pot ... | 2014 | 24489765 |
| genome sizes of nine insect species determined by flow cytometry and k-mer analysis. | the flow cytometry method was used to estimate the genome sizes of nine agriculturally important insects, including two coleopterans, five hemipterans, and two hymenopterans. among which, the coleopteran lissorhoptrus oryzophilus (kuschel) had the largest genome of 981 mb. the average genome size was 504 mb, suggesting that insects have a moderate-size genome. compared with the insects in other orders, hymenopterans had small genomes, which were averagely about ~200 mb. we found that the genome ... | 2016 | 27932995 |
| the oxidizing agent, paraquat, is more toxic to wolbachia than to mosquito host cells. | cultured cells provide an important in vitro system for examining metabolic interactions between the intracellular bacterium, wolbachia pipientis, and its insect hosts. to test whether wolbachia-associated changes in antioxidant activities could provide a tool to select for infected cells, we tested the effects of paraquat (pq) on aedes albopictus mosquito cells. like mammalian cells, mosquito cells tolerate pq over a wide range of concentrations, and for considerable lengths of time, depending ... | 2013 | 23719839 |
| rice stripe virus counters reduced fecundity in its insect vector by modifying insect physiology, primary endosymbionts and feeding behavior. | virus-vector relationships can be complex and diverse as a result of long-term coevolution. understanding these interactions is crucial for disease and vector management. rice stripe virus (rsv) is known to be transovarially transmitted within its vector, laodelphax striatellus, and causes serious rice stripe disease. in rsv-infected l. striatellus, we found contrasting changes in vector fecundity, physiology, primary endosymbionts (i.e. yeast-like symbionts, yls) and feeding behavior that can i ... | 2015 | 26211618 |
| transcriptome and comparative gene expression analysis of sogatella furcifera (horváth) in response to southern rice black-streaked dwarf virus. | the white backed planthopper (wbph), sogatella furcifera (horváth), causes great damage to many crops by direct feeding or transmitting plant viruses. southern rice black-streaked dwarf virus (srbsdv), transmitted by wbph, has become a great threat to rice production in east asia. | 2012 | 22558400 |
| roles of the laodelphax striatellus down syndrome cell adhesion molecule in rice stripe virus infection of its insect vector. | the arthropod down syndrome cell adhesion molecule (dscam) mediates pathogen-specific recognition via an extensive protein isoform repertoire produced by alternative splicing. to date, most studies have focused on the subsequent pathogen-specific immune response, and few have investigated the entry into cells of viruses or endosymbionts. in the present study, we cloned and characterized the cdna of laodelphax striatellus dscam (lsdscam) and investigated the function of lsdscam in rice stripe vir ... | 2016 | 26991800 |
| cloning, expression, purification, and insecticidal activity of a novel cry1na3 toxin from bacillus thuringiensis brc-zyr2. | bacillus thuringiensis produces a variety of insecticidal crystal proteins (icps). genome sequencing is a promising strategy for detecting and identifying b. thuringiensis icps, which are of great interest to the biocontrol field. in this study, a novel icp gene was cloned from b. thuringiensis brc-zyr2 based on genomic data from 454 gs-flx titanium sequencing and an analysis of the results using the b. thuringiensis toxin_scanner (http://bcam.hzaubmb.net/bttoxin_scanner/index.php). cry1na3 desi ... | 2016 | 27170729 |
| single and fused transgenic bacillus thuringiensis rice alter the species-specific responses of non-target planthoppers to elevated carbon dioxide and temperature. | the approval of transgenic bacillus thuringiensis (bt) rice by china was momentous for biotech crops, although it has yet to be approved for commercial production. non-target pest problems in rice paddies, such as the three ecologically similar species of planthoppers nilaparvata lugens, laodelphax striatellus and sogatella furcifera, could become increasingly serious under global climate change. fused (cry1ab/cry1ac) and single (cry1ab) transgenic bt rice were evaluated for effects on species-s ... | 2014 | 24136625 |
| infection rate assay by nested pcr and the phylogenetic analysis of himetobi p virus in the main pests of rice-wheat cropping systems. | himetobi p virus (hipv) is an ssrna in the family dicistroviridae that infects rice pests belonging to hemiptera. to determine its host range, a nested pcr method was designed to detect hipv in some of the main rice pests (hemiptera) in eastern china. the incidence of infection in the grain aphid sitobion avenae fabricius (hemiptera: aphididae) was low (3%), while high incidences of infection occurred in the planthoppers laodelphax striatellus (fallén) (hemiptera: delphacidae) (100%) and nilapar ... | 2015 | 26470259 |
| unusual characteristics of dicistrovirus-derived small rnas in the small brown planthopper, laodelphax striatellus. | in this study, sequences of small rna (srna) libraries derived from the insect vector laodelphax striatellus were assembled into contigs and used as queries for database searches. a large number of contigs were highly homologous to the genome sequence of an insect dicistrovirus, himetobi p virus (hipv). interestingly, hipv-derived srnas had a wide size distribution, and were relatively abundant throughout the 18-30 nt size range with only a slight peak at 22 nt. hipv srnas had a strong bias towa ... | 2014 | 24323637 |
| identification of himetobi p virus in the small brown planthopper by deep sequencing and assembly of virus-derived small interfering rnas. | profiling and assembly of virus-derived small interfering rnas (sirnas) using next-generation sequencing technologies have been very useful for identification and diagnosis of a number of plant and invertebrate viruses. in this work, we have conducted high-throughput pyrosequencing and bioinformatic analysis of the small brown planthopper (sbph, laodelphax striatellus), and these analyses unexpectedly showed that the himetobi p virus (hipv) was present in our laboratory cultures. hipv was also f ... | 2014 | 24239755 |
| rnai-mediated resistance to rice black-streaked dwarf virus in transgenic rice. | rice black-streaked dwarf virus (rbsdv), a member of the genus fijivirus in the family reoviridae, causes significant economic losses in rice production in china and many other asian countries. development of resistant varieties by using conventional breeding methods is limited, as germplasm with high level of resistance to rbsdv have not yet been found. one of the most promising methods to confer resistance against rbsdv is the use of rna interference (rnai) technology. rbsdv non-structural pro ... | 2017 | 27900537 |
| the speed of tubule formation of two fijiviruses corresponds with their dissemination efficiency in their insect vectors. | rice black-streaked dwarf virus (rbsdv) and southern rice black-streaked dwarf virus (srbsdv) are two closely related fijiviruses transmitted by the small brown planthopper (sbph) and white-backed planthopper (wbph), respectively. srbsdv has a latent period 4 days shorter than that of rbsdv, implying a more efficient spread in insect vector. currently, the mechanisms underlying this higher efficiency are poorly understood. however, our recent studies have implicated a role of virus induced tubul ... | 2016 | 27760544 |
| complete genomic sequence of maize rough dwarf virus, a fijivirus transmitted by the small brown planthopper. | the nucleotide sequences of the 10 genomic segments of an italian isolate of maize rough dwarf virus (mrdv) were determined. this first complete genomic sequence of mrdv will help understand the phylogenetic relationships among group 2 fijiviruses and especially the closely related rice black-streaked dwarf virus, which is also found to naturally infect maize. | 2016 | 26847903 |
| argonaute subfamily genes in the small brown planthopper, laodelphax striatellus (hemiptera: delphacidae). | argonaute (ago) proteins are essential catalytic components of the rna-induced silencing complex and play central roles in rna interference. using a combination of bioinformatics and rapid amplification of cdna ends (race) methods, putative ago subfamily members, ls-ago1 and ls-ago2, were cloned and characterized from the small brown planthopper, laodelphax striatellus. the open reading frame (orf) of ls-ago1 is 2,820 bp long, encoding a putative protein of 939 amino acid residues, and ls-ago2 c ... | 2016 | 26446351 |
| genetic analysis and molecular mapping of qtls for resistance to rice black-streaked dwarf disease in rice. | rice black-streaked dwarf disease, caused by rice black-streaked dwarf virus (rbsdv), is transmitted by small brown planthoppers (laodelphax striatellus fallén, sbph) and causes severe yield loss in epidemic years in china and other east asian countries. breeding for resistance to rbsdv is a promising strategy to control the disease. we identified tetep that showed resistance to rbsdv using a field test and artificial inoculation test. an evaluation of the resistance mechanism revealed that tete ... | 2015 | 26198760 |
| whole-genome expression analysis of rice black-streaked dwarf virus in different plant hosts and small brown planthopper. | rice black-streaked dwarf virus (rbsdv) can infect a number of gramineous plants and cause severe crop yield losses in southeast asian countries. the virus is transmitted by small brown planthopper (sbph) in a persistent circulative manner. the interactions between rbsdv and its different hosts remain unknown. besides, how the virus adjusts itself to infect different hosts is unclear. in the present study, the relative rna levels of the thirteen rbsdv genes in rice, maize, wheat, and sbph were m ... | 2015 | 26149652 |
| quantification of southern rice black streaked dwarf virus and rice black streaked dwarf virus in the organs of their vector and nonvector insect over time. | southern rice black streaked dwarf virus (srbsdv) and rice black streaked dwarf virus (rbsdv) are serious rice-infecting reoviruses, which are transmitted by different planthoppers in a persistent propagative manner. in this study, we quantitatively compared the spatial distribution of srbsdv and rbsdv contents over time in their vector and nonvector insects using real time-pcr. genome equivalent copies (gec) were assessed every 2 days from 0 to 14 days after a 3-days acquisition access period ( ... | 2015 | 26116274 |
| a simplified method for simultaneous detection of rice stripe virus and rice black-streaked dwarf virus in insect vector. | rice stripe virus (rsv) and rice black-streaked dwarf virus (rbsdv) are transmitted by their common vector small brown planthopper (sbph) that cause serious crop losses in china. a simple reverse transcription-pcr method was developed for the simultaneous detection of rsv and rbsdv in single sbph. three primers targeted to rsv-rna4 and rbsdv-s2 segments were designed to amplify respectively 1114-bp and 414-bp fragments in a reaction. the method is reliable, rapid and inexpensive for detecting th ... | 2015 | 25455902 |
| a novel, in vivo, indoor method to preserve rice black-streaked dwarf virus in small brown planthopper using wheat seedling as a bridge host. | rice black-streaked dwarf virus (rbsdv) naturally infects gramineae plants through small brown planthopper (sbph) as a vector. however, rbsdv cannot be transmitted to the sbph offspring through transovarian transmission. wheat plant, an important intermediate host in winter, is essential for the completion of the annual cycle of rbsdv in farm ecosystem. we developed a novel, in vivo, indoor method to preserve rbsdv in sbph using wheat seedling as a bridge host. the temperature range of 23-27°c w ... | 2014 | 25075933 |
| migration of rice planthoppers and their vectored re-emerging and novel rice viruses in east asia. | this review examines recent studies of the migration of three rice planthoppers, laodelphax striatellus, sogatella furcifera, and nilaparvata lugens, in east asia. laodelphax striatellus has recently broken out in jiangsu province, eastern china. the population density in the province started to increase in the early 2000s and peaked in 2004. in 2005, rice stripe virus (rsv) viruliferous rate of l. striatellus peaked at 31.3%. since then, rice stripe disease spread severely across the whole prov ... | 2013 | 24312081 |
| overexpression of rice black-streaked dwarf virus p7-1 in arabidopsis results in male sterility due to non-dehiscent anthers. | rice black-streaked dwarf virus (rbsdv), a member of the genus fijivirus in the family reoviridae, is propagatively transmitted by the small brown planthopper (laodelphax striatellus fallén). rbsdv causes rice black-streaked dwarf and maize rough dwarf diseases, which lead to severe yield losses in crops in china. although several rbsdv proteins have been studied in detail, the functions of the nonstructural protein p7-1 are still largely unknown. to investigate the role of the p7-1 protein in v ... | 2013 | 24260239 |
| southern rice black-streaked dwarf virus: a white-backed planthopper-transmitted fijivirus threatening rice production in asia. | southern rice black-streaked dwarf virus (srbsdv), a non-enveloped icosahedral virus with a genome of 10 double-stranded rna segments, is a novel species in the genus fijivirus (family reoviridae) first recognized in 2008. rice plants infected with this virus exhibit symptoms similar to those caused by rice black-streaked dwarf virus. since 2009, the virus has rapidly spread and caused serious rice losses in east and southeast asia. significant progress has been made in recent years in understan ... | 2013 | 24058362 |
| influence of rice black streaked dwarf virus on the ecological fitness of non-vector planthopper nilaparvata lugens (hemiptera: delphacidae). | rice black streak dwarf virus (rbsdv) is transmitted by the small brown planthopper (sbph), laodelphax striatellus (fallen). non-vector rice brown planthopper (bph), nilaparvata lugens (stål), shares the same host rice plants with sbph in paddy fields. the changes in nutritional composition of rice plants infected by rbsdv and the ecological fitness of bph feeding on the infected plants were studied under both artificial climate chamber and field conditions. contents of 16 detected amino acids a ... | 2014 | 23956237 |
| characterization of rice black-streaked dwarf virus- and rice stripe virus-derived sirnas in singly and doubly infected insect vector laodelphax striatellus. | replication of rna viruses in insect cells triggers an antiviral defense that is mediated by rna interference (rnai) which generates viral-derived small interfering rnas (sirnas). however, it is not known whether an antiviral rnai response is also induced in insects by reoviruses, whose double-stranded rna genome replication is thought to occur within core particles. deep sequencing of small rnas showed that when the small brown planthopper (laodelphax striatellus) was infected by rice black-str ... | 2013 | 23776591 |
| restriction of viral dissemination from the midgut determines incompetence of small brown planthopper as a vector of southern rice black-streaked dwarf virus. | southern rice black-streaked dwarf virus (srbsdv), a fijivirus, is transmitted by the white-backed planthopper in a persistent-propagative manner. in this study, we found that another planthopper species, the small brown planthopper (sbph), could acquire srbsdv but not transmit it. to identify the transmission barrier for srbsdv in sbphs, sequential infection by srbsdv in the organs of sbphs was studied with immunofluorescence for viral antigens. srbsdv initially entered the epithelial cells of ... | 2012 | 22683297 |
| evolution of rice stripe virus. | rice stripe virus (rsv) is an insect-borne tenuivirus of economical significance. it is endemic to the rice-growing regions of east asia and exhibits more genetic diversity in yunnan province of china. to gain more insights into the molecular epidemiology and evolution of rsv, recombination analyses were conducted and potential events were detected in each of the four rna segments of rsv. bayesian coalescent method was then applied to the time-stamped coding sequences of the cp gene. the nucleot ... | 2017 | 28189616 |
| rice stripe virus infection alters mrna levels of sphingolipid-metabolizing enzymes and sphingolipids content in laodelphax striatellus. | sphingolipids and their metabolites have been implicated in viral infection and replication in mammal cells but how their metabolizing enzymes in the host are regulated by viruses remains largely unknown. here we report the identification of 12 sphingolipid genes and their regulation by rice stripe virus in the small brown planthopper (laodelphax striatellus fallén), a serious pest of rice throughout eastern asia. according to protein sequence similarity, we identified 12 sphingolipid enzyme gen ... | 2017 | 28130458 |
| artificial feeding rice stripe virus enables efficient virus infection of laodelphax striatellus. | rice stripe virus (rsv), the causative agent of rice stripe disease, is transmitted by laodelphax striatellus in a persistent-propagative manner. efficient virus acquisition is primary for studies of virus transmission and virus-insect vector interactions. however, under greenhouse conditions, less than 30% of the l. striatellus population, on average, become viruliferous during feeding on rsv-infected plants. here, we explored a method for efficient rsv acquisition by feeding the insects with a ... | 2016 | 27283882 |
| differential proteomics profiling of the ova between healthy and rice stripe virus-infected female insects of laodelphax striatellus. | rice stripe virus-infected females of the small brown planthopper (sbph, laodelphax striatellus) usually lay fewer eggs with a longer hatch period, low hatchability, malformation and retarded or defective development compared with healthy females. to explore the molecular mechanism of those phenomena, we analyzed the differential proteomics profiling of the ova between viruliferous and healthy female insects using an isobaric tag for relative and absolute quantitation (itraq) approach. we obtain ... | 2016 | 27277140 |
| seasonal changes in the percentage of rice stripe virus viruliferous laodelphax striatellus (hemiptera: delphacidae) in paddy fields in japan. | rice stripe disease, which is caused by rice stripe virus (rsv), is one of the most serious viral diseases of rice. rsv is transmitted in a persistent manner bylaodelphax striatellus(fallén). the incidence of the disease can be estimated from the density of viruliferous vectors. understanding seasonal changes of the percentage of viruliferousl. striatelluscan facilitate forecasting and controlling the disease. in paddies, the percentage of viruliferous insects fluctuated in phase with the rate o ... | 2016 | 27099363 |
| viruliferous rate of small brown planthopper is a good indicator of rice stripe disease epidemics. | rice stripe virus (rsv), its vector insect (small brown planthopper, sbph) and climatic conditions in jiangsu, china were monitored between 2002 and 2012 to determine key biotic and abiotic factors driving epidemics of the disease. average disease severity, disease incidence and viruliferous rate of sbph peaked in 2004 and then gradually decreased. disease severity of rsv was positively correlated with viruliferous rate of the vector but not with the population density of the insect, suggesting ... | 2016 | 26898155 |
| organ-specific transcriptome response of the small brown planthopper toward rice stripe virus. | rice stripe virus (rsv) causes rice stripe disease and is transmitted by the small brown planthopper (laodelphax striatellus, sbph) in a persistent, circulative, and propagative manner. the alimentary canal and salivary gland of sbph play important roles in viral replication and transmission. however, little is known about the underlying molecular functions of these two organs in the interaction between rsv and sbph. in this study, organ-specific transcriptomes of the alimentary canal and saliva ... | 2016 | 26678499 |
| different pathogenicities of rice stripe virus from the insect vector and from viruliferous plants. | persistent plant viruses usually depend on insects for their transmission; they cannot be transmitted between plants or through mechanical inoculation. however, the mechanism by which persistent viruses become pathogenic in insect vectors remains unknown. in this study, we used rice stripe virus (rsv), its insect vector laodelphax striatellus and host plant (oryza sativa) to explore how persistent viruses acquire pathogenicity from insect vectors. rsv acquired phytopathogenicity in both the alim ... | 2016 | 26585422 |
| [stress effects of imidacloprid on rsv in rice plants]. | the rice stripe disease is a viral disease transmitted by small brown planthopper, laodelphax striatellus, which outbroke a few years ago in the yangtze river basin, especially jiangsu region, china. to study the effects of imidacloprid stress on rice stripe virus (rsv) in rice plants, the rice seedlings were treated with imidacloprid 1, 2, 3 and 4 times (b1, b2, b3 and b4), respectively, after artificial inoculation by l. striatellus for 48 h, and the expression levels of relative genes includi ... | 2014 | 25876413 |
| rice stripe tenuivirus nonstructural protein 3 hijacks the 26s proteasome of the small brown planthopper via direct interaction with regulatory particle non-atpase subunit 3. | the ubiquitin/26s proteasome system plays a vital role in regulating host defenses against pathogens. previous studies have highlighted different roles for the ubiquitin/26s proteasome in defense during virus infection in both mammals and plants, but their role in the vectors that transmit those viruses is still unclear. in this study, we determined that the 26s proteasome is present in the small brown planthopper (sbph) (laodelphax striatellus) and has components similar to those in plants and ... | 2015 | 25653432 |
| transovarial transmission of a plant virus is mediated by vitellogenin of its insect vector. | most plant viruses are transmitted by hemipteroid insects. some viruses can be transmitted from female parent to offspring usually through eggs, but the mechanism of this transovarial transmission remains unclear. rice stripe virus (rsv), a tenuivirus, transmitted mainly by the small brown planthopper (laodelphax striatellus), is also spread to the offspring through the eggs. here, we used the rsv-planthopper system as a model to investigate the mechanism of transovarial transmission and demonst ... | 2014 | 24603905 |
| nonstructural protein ns4 of rice stripe virus plays a critical role in viral spread in the body of vector insects. | rice stripe virus (rsv), a tenuivirus, is transmitted by small brown planthopper (sbph) in a persistent-propagative manner. in this study, sequential infection of rsv in the internal organs of sbph after ingestion of virus indicated that rsv initially infected the midgut epithelium, and then progressed to the visceral muscle tissues, through which rsv spread to the entire alimentary canal. finally, rsv spread into the salivary glands and reproductive system. during viral infection, the nonstruct ... | 2014 | 24523924 |
| comparative transcriptome analysis of two rice varieties in response to rice stripe virus and small brown planthoppers during early interaction. | rice stripe, a virus disease, transmitted by a small brown planthopper (sbph), has greatly reduced production of japonica rice in east asia, especially in china. although we have made great progress in mapping resistance genes, little is known about the mechanism of resistance. by de novo transcriptome assembling, we gained sufficient transcript data to analyze changes in gene expression of early interaction in response to sbph and rsv infection in rice. respectively 648 and 937 degs were detect ... | 2013 | 24358146 |
| investigation on subcellular localization of rice stripe virus in its vector small brown planthopper by electron microscopy. | rice stripe virus (rsv), which is transmitted by small brown planthopper (laodelphax striatellus fallén, sbph), has been reported to be epidemic and cause severe rice stripe disease in rice fields in many east asian countries, including china. investigation on viral localization in the vector is very important for elucidating transmission mechanisms of rsv by sbph. in this study, transmission electron microscopy and immuno-gold labeling technique were used to investigate the subcellular localiza ... | 2013 | 24139455 |
| transcription of orfs on rna2 and rna4 of rice stripe virus terminate at an auccggau sequence that is conserved in the genus tenuivirus. | rice stripe virus, the type member of the genus tenuivirus, has four genomic rnas. rnas 2-4 have an ambisense coding strategy and the noncoding intergenic regions (irs) separating the two orfs are thought to function in termination of transcription. sequencing the 3'-untranslated region of transcripts from rna2 and rna4 in virus-infected oryza sativa (the natural host), nicotiana benthamiana (an experimental host) and laodelphax striatellus (the vector), showed that the sequences of p2 and pc2 t ... | 2013 | 23624227 |
| detection of quantitative trait loci (qtls) for resistances to small brown planthopper and rice stripe virus in rice using recombinant inbred lines. | small brown planthopper (sbph) and rice stripe virus (rsv) disease transmitted by sbph cause serious damage to rice (oryza sativa l.) in china. in the present study, we screened 312 rice accessions for resistance to sbph. the indica variety, n22, is highly resistant to sbph. one hundred and eighty two recombinant inbred lines (rils) derived from a cross of n22 and the highly susceptible variety, ussr5, were used for quantitative trait locus (qtl) analysis of resistances to sbph and rsv. in a mod ... | 2013 | 23591851 |
| an insect cell line derived from the small brown planthopper supports replication of rice stripe virus, a tenuivirus. | a cell line from the small brown planthopper (sbph; laodelphax striatellus) was established to study replication of rice stripe virus (rsv), a tenuivirus. the sbph cell line, which had been subcultured through 30 passages, formed monolayers of epithelial-like cells. inoculation of cultured vector cells with rsv resulted in a persistent infection. during viral infection in the sbph cell line, the viral non-structural protein ns3 co-localized with the filamentous ribonucleoprotein particles of rsv ... | 2013 | 23468422 |
| surface display of rice stripe virus nsvc2 and analysis of its membrane fusion activity. | rice stripe virus (rsv) infects rice and is transmitted in a propagative manner by the small brown planthopper. how rsv enters an insect cell to initiate the infection cycle is poorly understood. sequence analysis revealed that the rsv nsvc2 protein was similar to the membrane glycoproteins of several members in the family bunyaviridae and might induce cell membrane fusion. to conveniently study the membrane fusion activity of nsvc2, we constructed cell surface display vectors for expressing nsv ... | 2012 | 22492001 |
| analysis of rice stripe virus whole-gene expression in rice and in the small brown planthopper by real-time quantitative pcr. | rice stripe virus (rsv) is a typical member of the tenuivirus genus, which is transmitted mainly by the small brown planthopper (sbph) in a persistent, circulative-propagative manner. at present, expression levels of viral genes in rice and in sbph remain unclear. in this study, we investigated rsv gene expression in rsv-infected rice tissues and in viruliferous sbph using a sensitive and reliable real-time quantitative pcr (q-pcr) method. the results revealed that ns3 gene exhibited the highest ... | 2012 | 22404613 |
| pichia anomala, a new species of yeast-like endosymbionts and its variation in small brown planthopper (laodelphax striatellus). | yeast-like symbionts (yls) are endosymbionts that promote the growth of delphacid planthoppers (hemiptera: delphacidae), some of which are pests on cultivated rice. identification and characterization of yls growth can be helpful for pest control, because it has been demonstrated that there is a variety of yls in rice planthopper and they affected the planthopper's growth and virulence to plant hosts. so, elucidation of the species of yls in planthopper is crucial for exploiting a new way to con ... | 2015 | 25499750 |
| knockdown of a putative halloween gene shade reveals its role in ecdysteroidogenesis in the small brown planthopper laodelphax striatellus. | ecdysteroid hormone 20-hydroxyecdysone (20e) plays fundamental roles in insect development and reproduction, whereas the primary role of ecdysone (e) is the precursor for 20e. a cytochrome p450 monooxygenase (cyp), encoded by a halloween gene shade (shd, cyp314a1), catalyzes the conversion of e into 20e in representative insect species in diptera, lepidoptera and orthoptera. we describe here the cloning and characterization of lsshd in a hemipteran insect species, the small brown planthopper lao ... | 2013 | 24055487 |
| chitin synthase 1 gene and its two alternative splicing variants from two sap-sucking insects, nilaparvata lugens and laodelphax striatellus (hemiptera: delphacidae). | chitin synthase (chs) is an enzyme that is required for chitin formation in insect cuticles and other tissues. in this study, chs genes from two destructive rice insect pests, the brown planthopper nilaparvata lugens and the small brown planthopper laodelphax striatellus, were cloned. phylogenetic analysis showed that these genes belonged to class chs1 of the chs gene family. most insects possess two chs genes (chs1 and chs2); however, genome and transcriptome searches showed that n. lugens poss ... | 2012 | 22634163 |
| knockdown of nadph-cytochrome p450 reductase results in reduced resistance to buprofezin in the small brown planthopper, laodelphax striatellus (fallén). | nadph-cytochrome p450 reductase (cpr) plays an important role in cytochrome p450 function, and cpr knockdown in several insects leads to increased susceptibility to insecticides. however, a putative cpr gene has not yet been fully characterized in the small brown planthopper laodelphax striatellus, a notorious agricultural pest in rice that causes serious damage by transmitting rice stripe and rice black-streaked dwarf viruses. the objective of this study was to clone the cdna and to knock down ... | 2016 | 26821654 |
| rna interference-mediated silencing of a halloween gene spookier affects nymph performance in the small brown planthopper laodelphax striatellus. | post-embryonic development of insects is highly dependent on ecdysteroid hormone 20-hydroxyecdysone. halloween gene spookier (spok, cyp307a2) has been documented to be involved in ecdysteroidogenesis in drosophila melanogaster and bombyx mori. we describe here the cloning and characterization of halloween gene spookier (lsspok, lscyp307a2) in the small brown planthopper laodelphax striatellus, a hemipteran insect species. lsspok has three insect-conserved p450 motifs, that is, helix-k, perf moti ... | 2015 | 24282064 |
| bt rice expressing cry2aa does not cause direct detrimental effects on larvae of chrysoperla sinica. | to assess the potential effects of cry2aa-expressing insect-resistant bt rice on chrysoperla sinica larvae, we conducted two tritrophic bioassays using a non-target (laodelphax striatellus) and a target herbivore (chilo suppressalis) as prey. none of the tested life-table parameters of c. sinica did differ when fed with l. striatellus nymphs reared on either bt or control rice plants. similarly, c. sinica larval survival and development were not affected when fed c. suppressalis larvae that were ... | 2013 | 24057602 |
| effects of high temperature on life history traits and heat shock protein expression in chlorpyrifos-resistant laodelphax striatella. | the resistance of the small brown planthopper (sbph), laodelphax striatella, to insecticides has been widely found in china, and has posed serious problems to efforts to control the pest. to determine the costs and benefits of resistance, the life tables of chlorpyrifos-resistant and -susceptible strains were constructed at 24 and 30°c. the results showed the resistant sbph (yn-cpf) had lower fitness at 24°c, but slightly higher fitness at 30°c compared to the susceptible sbph. transcriptomic an ... | 2017 | 28187833 |
| differential mechanisms of action of the novel γ-aminobutyric acid receptor antagonist ectoparasiticides fluralaner (a1443) and fipronil. | fluralaner (a1443) is an isoxazoline ectoparasiticide that is a novel antagonist of γ-aminobutyric acid (gaba) receptors (gabars), with a potency comparable to that of fipronil, a phenylpyrazole ectoparasiticide. to clarify the biological effectiveness of fluralaner against fipronil-resistant pests, differences in the actions of fluralaner and fipronil on gabars that possess resistance to dieldrin (rdl)-type mutations were evaluated. | 2015 | 24591229 |
| complete mitochondrial genome of the small brown planthopper, laodelphax striatellus (delphacidae: hemiptera), with a novel gene order. | we determined the first complete mitochondrial genome (mitogenome) sequence from a representative of the insect family delphacidae, laodelphax striatellus. the 16,513 bp long l. striatellus mitogenome encodes 13 putative proteins, two ribosomal rnas, and 22 transfer rnas, and contains a putative control region (or a+t-rich region). the nucleotide composition is biased toward adenine and thymine (77.2% a+t), and the amino acid composition is affected to a similar degree by the at mutational bias. ... | 2009 | 19968473 |
| first report of cheiloneurus exitiosus (perkins, 1906) and helegonatopus dimorphus (hoffer, 1954) (hymenoptera: encyrtidae) from japan, with remarks on their abundance in rice paddies. | encyrtid secondary parasitoids of delphacidae have not been recorded in japan. however, they may play an important role in the rice ecosystem because they can reduce the number of dryinidae, the natural enemies of rice planthoppers. | 2016 | 27660532 |
| development of a simplified rt-pcr without rna isolation for rapid detection of rna viruses in a single small brown planthopper (laodelphax striatellus fallén). | the small brown planthopper (sbph) is an important pest of cereal crops and acts as a transmission vector for multiple rna viruses. rapid diagnosis of virus in the vector is crucial for efficient forecast and control of viral disease. reverse transcription polymerase chain reaction (rt-pcr) is a rapid, sensitive and reliable method for virus detection. the traditional rt-pcr contains a rna isolation step and is widely used for virus detection in insect. however, using the traditional rt-pcr for ... | 2017 | 28468626 |
| phases of maize rough dwarf virus multiplication in the vector laodelphax striatellus (fallén). | | 1970 | 5422718 |