| mapping of antigenic sites on the major inner capsid protein of avian rotavirus using an escherichia coli expression system. | the cdna encoding the vp6 gene of avian rotavirus po-13 strain was inserted into the bacterial expression vector pet-3a. upon isopropyl-1-thio-beta-d-galactoside induction, the e. coli bl21 (de3) harboring the vector containing cdna of the vp6 gene produced an approximately 45-kda polypeptide, which reacted with rabbit serum against po-13 strain in western blotting. to study the antigenic sites on vp6, various deletion mutants were constructed, expressed in e. coli and the reactivity with antige ... | 1996 | 8973528 |
| complete nucleotide sequence of a group a avian rotavirus genome and a comparison with its counterparts of mammalian rotaviruses. | the nucleotide sequences encoding four structural proteins (vp1-4) and six nonstructural proteins (nsp1-6) of avian rotavirus po-13 were determined. based on the results of earlier sequencing studies [ito et al., 1995, sequence analysis of cdna for the vp6 protein of group a avian rota viruses. arch. vriol. 140, 605-612; rohwedder et al., 1997, chicken rotavirus ch-1 shows a second type of avian vp6 gene, virus genes 15, 65-71; rohwedder et al., 1997, bovine rotavirus 993/83 shows a third subtyp ... | 2001 | 11325467 |