overview of studies on liver carcinogenesis in english sole from puget sound; evidence for a xenobiotic chemical etiology. ii: biochemical studies.the levels of aromatic hydrocarbons in sediments of puget sound, washington, are positively correlated with the prevalence of hepatic neoplasms and related lesions in english sole (parophrys vetulus). to investigate the biochemical processes involved in chemical carcinogenesis in fish from puget sound, we have studied the uptake, activation, and detoxication of polycyclic aromatic hydrocarbons (pahs) in english sole, and have compared these data to pah metabolism in a related species, starry flo ...19902360039
chemical carcinogenesis in feral fish: uptake, activation, and detoxication of organic xenobiotics.the high prevalence of liver neoplasms in english sole (parophrys vetulus) and substantially lower prevalence of neoplasms in a closely related species, starry flounder (platichthys stellatus) captured from industrialized waterways, provide a unique opportunity to compare biochemical processes involved in chemical carcinogenesis in feral fish species. because levels of aromatic hydrocarbons (ahs) in urban sediments are correlated with prevalences of liver neoplasms in english sole, we have initi ...19873297658
volume-sensitive taurine transport in fish erythrocytes.taurine plays an important role in cell volume regulation in both vertebrates and invertebrates. erythrocytes from two euryhaline fish species, the eel (anguilla japonica) and the starry flounder (platichthys stellatus) were found to contain high intracellular concentrations of this amino acid (approximately equal to 30 mmol per liter of cell water). kinetic studies established that the cells possessed a saturable high-affinity na+-dependent beta-amino-acid transport system which also required c ...19873585985
comparative metabolism of benzo(a)pyrene and covalent binding to hepatic dna in english sole, starry flounder, and rat.metabolism of benzo(a)pyrene (bap) in vivo and in vitro was studied using two benthic fish species, english sole (parophrys vetulus) and starry flounder (platichthys stellatus), and sprague-dawley rats. at 24 h after administration of bap (7.9 mumol/kg of body weight) to fish either p.o. (experiment 1) or i.p. (experiment 2), the specific activity of binding of bap metabolites to hepatic dna (pmol of bap equivalent per mg of dna) was higher in sole [2.1 in experiment 1; 28 +/- 5 (se) in experime ...19863731058
cryotoxicity of antifreeze proteins and glycoproteins to spinach thylakoid membranes--comparison with cryotoxic sugar acids.we have used thylakoids from spinach (spinacia oleracea l.) chloroplasts to test the effects of antifreeze proteins (afp) from the starry flounder (platichthys stellatus; afp-sf) and from the antarctic eel pout (austrolycichthys brachycephalus; afp-ab), and antifreeze glycoproteins (afgp) from the antarctic fish dissostichus mawsoni on biological membranes during freezing. freeze-thaw damage, measured as the release of the lumenal protein plastocyanin from the thylakoid vesicles, was strongly in ...19938452860
trimethylamine oxide and urea synthesis in rainbow smelt and some other northern fishes.trimethylamine oxide (tmao) and urea levels in the blood of rainbow smelt, osmerus mordax, were previously shown to increase dramatically in winter, but the means by which these osmolytes are acquired has remained unclear. in this study, i show that the smelt can synthesize tmao via liver trimethylamine oxidase activity and thus are not completely dependent on a dietary source of tmao. cold-acclimatized pacific herring, clupea harengus, were also found to have high levels of tmao in the blood, w ...19989754528
isolation and characterization of a rhabdovirus from starry flounder (platichthys stellatus) collected from the northern portion of puget sound, washington, usa.the initial characterization of a rhabdovirus isolated from a single, asymptomatic starry flounder (platichthys stellatus) collected during a viral survey of marine fishes from the northern portion of puget sound, washington, usa, is reported. virions were bullet-shaped and approximately 100 nm long and 50 nm wide, contained a lipid envelope, remained stable for at least 14 days at temperatures ranging from -80 to 5 degrees c and grew optimally at 15 degrees c in cultures of epithelioma papulosu ...200414769907
novel l-amino acid oxidase with antibacterial activity against methicillin-resistant staphylococcus aureus isolated from epidermal mucus of the flounder platichthys produce mucus substances as a defensive outer barrier against environmental xenobiotics and predators. recently, we found a bioactive protein in the mucus layer of the flounder platichthys stellatus, which showed antibacterial activity against staphylococcus epidermidis, staphylococcus aureus and methicillin-resistant s. aureus. in this study, we isolated and identified the antibacterial protein from the mucus components of p. stellatus using a series of column chromatography steps. we then ...201020015076
metabolic activation and covalent binding of benzo[a]pyrene to deoxyribonucleic acid catalyzed by liver enzymes of marine fish.metabolic activation and covalent binding of benzo[a]pyrene (bp) to deproteinized salmon sperm dna by supernatant fractions (10,000 g) of liver homogenates isolated from untreated, 3-methylcholanthrene (3-mc) or bp-treated starry flounder (platichthys stellatus) and coho salmon (oncorhynchus kisutch) were investigated. the influence of temperature, ph, time and concentrations of protein, bp, nadph and dna on covalent binding was investigated to obtain optimum conditions for in vitro binding (pmo ...198020227952
[ecological groups of flatfishes of peter the great bay (japan sea) based on the analysis od species composition of myxosporidian parasites].comparison of myxosporidian species compositions in different flatfish species fro peter the great bay showed that the flatfishes should be divided into three ecological groups--deep water (scaly-eyed plaice acanthopsetta nadeshnyi, korean flounder glyptocephalus stelleri, flatheaded flounder hippoglossoides dubius, and pinewood flounder cleisthenes herzensteini), relatively coastal (yellow-finned sole limanda aspera and brown sole pseudopleuronectes herzensteini), and coastal (japanese flounder ...201020536006
comparison of the effects of dietary single and multi-probiotics on growth, non-specific immune responses and disease resistance in starry flounder, platichthys 8-week feeding trial was conducted to evaluate the effects of dietary probiotics on growth performance and non-specific immune responses in starry flounder, platichthys stellatus. fish averaging 46.5 ± 0.65 g (mean ± sd) were fed one of the six experimental diets; one control (cont), and five other diets were prepared by supplementing single-probiotics 1 (bacillus subtilis; sp1, 2 × 10(9) cfu kg(-1) diet), single-probiotics 2 (bacillus licheniformis; sp2, 2 × 10(9) cfu kg(-1) diet), multi-pro ...201627818339
the diversity of microsporidia in parasitic copepods (caligidae: siphonostomatoida) in the northeast pacific ocean with description of facilispora margolisi n. g., n. sp. and a new family facilisporidae n. fam.three distinct microsporidia were identified from parasitic copepods in the northeast pacific ocean. sequencing and phylogenetic analysis of a partial small subunit ribosomal rna gene (ssu rdna) sequence identified a genetically distinct variety of desmozoon lepeophtherii from lepeophtheirus salmonis on cultured atlantic salmon salmo salar, and this was confirmed by transmission electron microscopy. phylogenetic analysis resolved the ssu rdna sequence of the second organism in a unique lineage t ...201222452386
recombinant production and evaluation of an antibacterial l-amino acid oxidase derived from flounder platichthys produce mucus substances as a defensive outer barrier against several bacterial infections. we have recently identified an antibacterial l-amino acid oxidase (pslaao1) in the mucus layer of the flounder platichthys stellate. in this study, the antibacterial protein pslaao1 was expressed as a secretory bioactive recombinant protein in the methylotrophic yeast pichia pastoris. the recombinant pslaao1 inhibited the growth of bacteria to the same levels as native pslaao1 present in mucus. in pa ...201525661816
streptococcous parauberis infection in starry flounder, platichthys stellatus: characterization of innate immune responses following experimental infection.streptococcus parauberis causing systemic infections has been recognized as a major bacterial disease in olive flounder paralichthys olivaceus in south korea. although an emerging outbreak of s. parauberis has affected heavily farmed fish species starry flounder platichthys stellatus, no study of the innate immune responses and pathogenic mechanisms in starry flounder is available. in the present study, starry flounder were intraperitoneally challenged with four s. parauberis strains to investig ...201323707742
characterization of genes encoding prolactin and prolactin receptors in starry flounder platichthys stellatus and their expression upon acclimation to freshwater.this study aims to investigate the genes encoding prolactin (prl) and prolactin receptors (prlr) and their tissue-specific expression in starry flounder platichthys stellatus. starry flounder prl gene consisting of five exons encodes an orf of 212 amino acid residue comprised of a putative signal peptide of 24 amino acids and a mature protein of 188 amino acids. it showed amino acid identities of 73 % with tuna thunnus thynnus, 71 % with black porgy acanthopagrus schlegelii, 69 % with nile tilap ...201322843312
conservation of a glutathione s-transferase in marine and freshwater fish.we have previously reported the isolation and cloning of glutathione s-transferase (gst) cdnas from two marine fish, english sole (pleuronectes vetulus) and starry flounder (platichthys stellatus), that exhibited > 95% identity to plaice (pleuronectes platessa) gst-a aquatic toxicol., 44, 171-182]. in the present study, we have used reverse transcription-polymerase chain reaction (rt-pcr) analysis to isolate a 471 nucleotide gst-like cdna from largemouth bass (micropterus salmoides) liver. seque ...200311460686
a study of the lethal and sublethal toxicity of polyphase p-100, an antisapstain fungicide containing 3-iodo-2-propynyl butyl carbamate (ipbc), on fish and aquatic invertebrates.the acute toxicity of polyphase p-100, an antisapstain wood preservative that contains 97% 3-iodo-2-propynyl butyl carbamate (ipbc), was determined for three species of fish (coho salmon, rainbow trout, and starry flounder) and three species of aquatic invertebrates (daphnia magna, hyalella azteca, and neomysis mercedis). the 96-h lc50 values for the various fish species exposed to polyphase p-100 ranged from 95 ppb for coho smolts (oncorhynchus kisutch) to 370 ppm for juvenile starry flounder ( ...19989732479
metabolic effects of exercise in the golden fish salminus maxillosus "dourado" (valenciennes, 1849).strenuous exercise in fish is usually a consequence of migration, reproduction, and spawning. varying among fishes, this kind of stress is associated with blood glucose and lactate increase, in relation to which two major groups are distinguishable: the "lactate releasers" and "non-lactate releasers". unlike strenuous exercise, sustained swimming imposes a variety of effort that results in distinct kinetic types of blood lactate and glucose. compared to platichthys stellatus and oncorhynchus myk ...200415620004
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