| identification of four vp4 serological types (p serotypes) of bovine rotavirus using viral reassortants. | a series of five reassortant viruses each containing the vp4 gene of a distinct bovine rotavirus and the vp7 gene of human rotavirus strain st3 was prepared, and antisera to these were produced in rabbits. in neutralization tests, these antisera allowed the differentiation of the five original strains (from three different vp7 or g serotypes) into three or possibly four vp4 or p serotypes. all of a further seven bovine rotavirus strains adapted to cell culture were successfully typed by these an ... | 1992 | 1328488 |
| comparative amino acid sequence analysis of vp4 for vp7 serotype 6 bovine rotavirus strains ncdv, b641, and uk. | in a previous study (s. zheng, g. n. woode, d. r. melendy, and r. f. ramig, j. clin. microbiol. 27:1939-1945, 1989), it was predicted that the vp7 serotype 6 bovine rotavirus strains ncdv and b641 do not share antigenically similar vp4s. in this study, gene 4 and the vp7 gene of b641 were sequenced, and the amino acid sequences were deduced and compared with those of ncdv and bovine rotavirus strain uk. amino acid sequence homology in vp7 between the three strains was greater than 94%, confirmin ... | 1991 | 1654450 |
| serological and genetic characterization of bovine rotaviruses in thailand by elisa and rna-rna hybridization: detection of numerous non-serotype 6 strains. | a total of 62 fecal specimens positive for rotavirus were collected from diarrheic cows in thailand in 1988 and 1989. the antigenic properties of rotaviruses in stool were examined by enzyme-liked immunosorbent assays using specific monoclonal antibodies directed at vp4, vp6 or vp7: all the bovine rotavirus strains were determined as subgroup i; none of the strains were reactive with serotype 6-specific monoclonal antibody; and different reactivities of the bovine strains with two anti-vp4 monoc ... | 1990 | 2098924 |
| molecular characterization by rna-rna hybridization of a serotype 8 human rotavirus with "super-short" rna electropherotype. | a human rotavirus isolate (the 69m strain) with a "super-short" rna electropherotype has been classified as serotype 8, a new human serotype [matsuno et al., 1985]rna-rna hybridization using 32p-labeled transcription probes was used to assess the genetic relatedness of the 69m strain to a panel of human and animal rotaviruses. the 69m strain showed a medium level of homology with subgroup 1, serotype 2 human rotaviruses with short rna electropherotypes. however, the 69m strain was not significan ... | 1990 | 2156004 |
| presence of three p types (vp4 serotypes) and two g types (vp7 serotypes) among bovine rotavirus strains. | cross neutralization tests with a panel of rotavirus strains representing previously described nine vp7 (g) serotypes revealed that bovine rotavirus strain kk-3, a prototype japanese bovine serotype 2, belonged to a new serotype (g10), confirming and extending the recent report of snodgrass et al. [j. clin. microbiol. 28: 504-507 (1990)] which showed that hyperimmune serum to the kk-3 strain neutralized the b223 strain, a proposed type strain of g10. further antigenic characteristics of the kk-3 ... | 1990 | 2175589 |
| prevalence of antibodies to four bovine rotavirus strains in different age groups of cattle. | neutralizing antibody titers to four bovine rotavirus strains, representing three serotypes, were measured in 160 sera from cattle of different age groups. age-specific seroprevalence analysis revealed serotype 6, represented by bovine rotavirus (brv) ncdv, as the predominant rotavirus serotype infecting german cattle and serotype 10, represented by brv v1005, as the least prominent. infections with serotype 8, represented by brv 678, occurred with intermediate frequency. antibodies of young cal ... | 1990 | 2177934 |
| comparative studies of the antigenic polypeptide species vp4, vp6, and vp7 of three strains of bovine rotavirus. | three bovine rotavirus strains belonging to two distinct serotype groups, serotype 6 (ncdv and b641) and b223, distinct from the other six mammalian rotavirus serotypes but not yet assigned to a serotype group, were compared with each other and with canine rotavirus (k9, serotype 3) by studying the properties of their cognate polypeptide species vp4, vp6, and vp7. the three viruses showed distinct differences in the polyacrylamide gel electrophoretic migration rates of protein species vp4 and vp ... | 1989 | 2476464 |
| antigenic and molecular characterization of bovine rotaviruses isolated in japan. | japanese bovine rotavirus isolates, which fall into two different serotypes, were shown to belong to subgroup i and to have long rna electrophoretypes. this study confirmed the distinction of two serotypes on the basis of a greater than 20-fold difference in neutralization titres between the homologous and heterologous reactions; however, significant one-way cross-neutralization was observed between one of the strains with bovine serotype 2 and antisera to strains with bovine serotype 1 (serotyp ... | 1989 | 2549598 |
| serological characterization of bovine rotaviruses isolated from dairy and beef herds in argentina. | bovine rotaviruses isolated from beef and dairy herds in argentina were serotyped by the immunoperoxidase focus reduction assay as previously described (g. gerna, m. battaglia, g. milenesi, n. passarani, e. percivalle, and e. cattaneo, infect. immun. 43:722-729, 1984). three strains from beef herds were related to the uk and ncdv bovine rotavirus strains defined as serotype 6 (y. hoshino, r. g. wyatt, h. b. greenberg, j. flores, and a. z. kapikian, j. infect. dis. 149:694-702, 1984). two other s ... | 1989 | 2553769 |
| isolation of a bovine rotavirus with a "super-short" rna electrophoretic pattern from a calf with diarrhea. | a rotavirus with a "super-short" rna electropherotype was isolated from a calf with diarrhea and was designated vmri strain. segments 10 and 11 of this rotavirus migrated more slowly than did those of bovine rotavirus strains ncdv, b641, and b223. the electrophoretic pattern of the vmri strain was similar to that reported for rotaviruses with super-short rna electropherotypes from humans and rabbits. northern (rna) blot hybridization indicated that gene 11 of the vmri strain was altered and migr ... | 1988 | 2846639 |
| genetic relatedness among animal rotaviruses. | the genomic relatedness among representative rotavirus strains was examined by employing cross-hybridization techniques. single stranded (ss) rna prepared by in vitro transcription of purified rotavirus particles and labeled with either 32p or 125i was hybridized to denatured genomic, double stranded (ds) rnas. the hybrids formed were analyzed by polyacrylamide gel electrophoresis (page) or by testing their sensitivity to digestion with single strand specific nuclease (s-1 nuclease). a relativel ... | 1986 | 3004389 |
| reassortant rotaviruses containing structural proteins vp3 and vp7 from different parents induce antibodies protective against each parental serotype. | genetic studies of reassortant rotaviruses have demonstrated that gene segments 4 and 9 each segregate with the serotype-specific neutralization phenotype in vitro. reassortant rotaviruses derived by coinfection of ma-104 cells with the simian strain sa11 and the antigenically distinct bovine strain ncdv were used to determine which viral genes coded for proteins which induced a protective immune response in vivo. in addition, reassortant rotaviruses containing only the gene segment 4 or 9 prote ... | 1986 | 3021983 |
| a study of cytopathic rotavirus strains isolated from calves with acute enteritis. | nine cytopathic bovine rotavirus strains were isolated in ma-104 cell cultures from fecal specimens of dairy calves suffering from diarrhea. isolation of the virus was accomplished from three outbreaks which occurred on dairy farms located in central and southern italy. fecal suspensions were treated with a high concentration (1000 micrograms/ml) of trypsin, and inoculated into ma-104 cell cultures grown out in eagle's minimum essential medium (mem) containing 5 micrograms/ml of the enzyme. cyto ... | 1983 | 6313289 |
| isolation of new serotypes of bovine rotavirus. brief report. | two cytopathogenic bovine rotavirus strains were isolated. the infectivity of both isolates was relatively stable at ph 3.0 and resistant to ether. their replication was not affected by 5-iodo-2'-deoxyuridine. the new isolates shared immunofluorescent antigen with the lincoln strain of bovine rotavirus. by neutralization test, however, these strains were clearly distinguished from one another. | 1983 | 6316874 |
| prevalence of neutralizing antibodies to 9 rotavirus strains representing 7 g-serotypes in sheep sera. | neutralizing antibodies to 9 rotavirus strains representing serotypes g1, g3, g5, g6, g8, g9, and g10 were investigated in 212 ovine serum samples from 3 age groups, 1-week-old lambs, 2- to 3-months-old lambs and adult sheep. all sera from 1-week-old lambs had neutralizing antibodies to all 9 rotavirus strains. both neutralizing antibody titers and prevalences to all 9 strains markedly decreased in the 2- to 3-months-old lamb group and increased again in the adult sheep group. also, adult sheep ... | 1995 | 7483248 |
| determination of bovine rotavirus g and p serotypes by polymerase chain reaction. | among bovine rotaviruses there are two major g serotypes (g6 and g10) and three p serotypes (p1, p5, and p11, each of which is defined on the basis of the vp4 antigenic specificity of ncdv, uk and kk-3, respectively). the nucleotide sequence of a p11 gene (kk-3) was determined. the predicted kk-3 vp4 contained 772 amino-acids and showed 96% amino-acid identity with b223 vp4, an american prototype of p11 bovine rotavirus. comparative analysis of the genes determining the g and p serotypes of bovi ... | 1993 | 8232344 |
| two-way cross-neutralization mediated by a shared p (vp4) serotype between bovine rotavirus strains with distinct g (vp7) serotypes. | among bovine rotavirus strains, there are three g serotypes (g6, g8, and g10) and three p (vp4) serotypes (pb1, pb2, and pb3, which are defined on the basis of strains ncdv, uk, and b223, respectively). plaque reduction neutralization assays with hyperimmune antisera disclosed two-way antigenic relationships of strain kn-4 with strain kk-3 (g10, pb3) as well as with strains ncdv (g6, pb1) and 0510 (g6, pb2). neutralization assays with monoclonal antibodies specific for g6, g10, and pb3 revealed ... | 1993 | 8381812 |
| independent segregation of the vp4 and the vp7 genes in bovine rotaviruses as confirmed by vp4 sequence analysis of g8 and g10 bovine rotavirus strains. | we determined the complete nucleotide sequences of the vp4 genes of five bovine rotavirus strains (a5, 61a, a44, b223 and kk3). the deduced vp4 amino acid sequence of strain a5 with g8 serotype specificity is 91.1% identical to that of strain ncdv of the serotype g6, and strain 61a with g10 serotype specificity has a vp4 amino acid sequence (95.2% identity) similar to that of the uk strain of the g6 serotype. in contrast, the vp4 amino acid sequences of strains a44, b223 and kk3 of the g10 serot ... | 1993 | 8389807 |
| review of rotavirus vaccine trials in finland. | oral rotavirus vaccines, including bovine rotavirus strains rit 4237 and rit 4256, rhesus rotavirus (rrv) vaccine, rhesus-human rotavirus vaccine reassortants (d x rrv, ds-1 x rrv, and tetravalent rrv), and human nursery rotavirus strain m37, have been evaluated in 5353 finnish infants for safety, immunogenicity, and efficacy against rotavirus gastroenteritis. bovine rotavirus vaccines were nonreactogenic in infants, whereas rrv-based and m37 vaccines were occasionally associated with febrile re ... | 1996 | 8752295 |
| the vp4 and vp7 of bovine rotavirus vmri are antigenically and genetically closely related to p-type 5, g-type 6 strains. | we have previously reported the isolation of a bovine rotavirus, designated vmri, with a super-short electropherotype. we have characterized this strain further as it has shown antigenic differences with the prototype g6 strain ncdv-lincoln. in this communication, we report the antigenic and molecular characterization and the nucleotide sequence of the vp4 and vp7 genes of this strain. virus neutralization tests indicated 2- to 13-fold differences in the titers between ncdv-lincoln, b641 and vmr ... | 1996 | 8870187 |
| detection of group a rotavirus strains circulating in calves in tunisia. | faecal samples were collected from 89 dairy calves to determine the prevalence of rotavirus infection in tunisia and the genomic diversity of bovine rotavirus strains. after screening of all faecal samples by enzyme-linked immunosorbent assay, rotavirus strains were analysed by rna polyacrylamide gel electrophoresis and characterized antigenically by monoclonal antibodies to the vp6 subgroup. the vp7 genotype was determined by nested rt-pcr. of the 89 calves tested, 27 (30%) were positive for ro ... | 2005 | 15703001 |
| a rotavirus strain isolated from pig-tailed macaque (macaca nemestrina) with diarrhea bears a p6[1]:g8 specificity. | a distinct rotavirus strain (ptrv) was isolated in cell cultures from a stool sample obtained from a diarrheic 3-year-old female pig-tailed macaque (macaca nemestrina) that was born at the breeding colony of the university of washington in seattle. unlike other known simian rotavirus strains including vervet monkey rotavirus sa11 which bears p5b[2]:g3 or p6[1]:g3 specificity, rhesus monkey rotavirus mmu18006 with p5b[3]:g3 specificity, pig-tailed macaque rotavirus yk-1 with p[3]:g3 specificity a ... | 2006 | 16242747 |
| molecular characterisation and analysis of bovine rotavirus strains circulating in ireland 2002-2004. | one hundred and two faecal samples were collected from calves diagnosed with rotavirus infection, in the southern region of ireland, from 2002 to 2004. ninety one percent (n=93) were confirmed positive for rotavirus, using latex agglutination and enzyme linked immunosorbent assay (elisa) methods. determination of the g- and p-types was carried out using nested reverse transcriptase polymerase chain reaction (nrt-pcr). g6 was the most prevalent genotype, accounting for 80.6% (75/93), g10 accounte ... | 2006 | 16844325 |
| molecular characterization of novel g5 bovine rotavirus strains. | group a rotaviruses are a major cause of acute gastroenteritis in young children as well as many domestic animals. the rotavirus genome is composed of 11 segments of double-stranded rna and can undergo genetic reassortment during mixed infections, leading to progeny viruses with novel or atypical phenotypes. the aim of this study was to determine if the bovine group a rotavirus strains kj44 and kj75, isolated from clinically infected calves, share genetic features with viruses obtained from hete ... | 2006 | 16928963 |
| molecular characterization of bovine rotavirus strains circulating in northern italy, 2003-2005. | a total of 232 stools collected from calves with rotavirus infection in herds located in northern italy from 2003 to 2005 was investigated. determination of the rotavirus g and p types was carried out using nested rt-pcr. g6 was the most prevalent genotype, accounting for 78.5% of samples, g10 accounted for 9.9% of samples and viruses of g8 type were found in 4.7% of samples. in 3% of samples, viruses were not classified due to concomitant infection with more g type strains, whereas viruses in 3 ... | 2008 | 18191347 |
| full genome-based classification of rotaviruses reveals a common origin between human wa-like and porcine rotavirus strains and human ds-1-like and bovine rotavirus strains. | group a rotavirus classification is currently based on the molecular properties of the two outer layer proteins, vp7 and vp4, and the middle layer protein, vp6. as reassortment of all the 11 rotavirus gene segments plays a key role in generating rotavirus diversity in nature, a classification system that is based on all the rotavirus gene segments is desirable for determining which genes influence rotavirus host range restriction, replication, and virulence, as well as for studying rotavirus epi ... | 2008 | 18216098 |
| [nucleotide sequence analysis of the nsp4 gene from group a rotaviruses isolated in nizhni novgorod]. | the complete nucleotide sequences of the nsp4 gene from russian isolates of group a rotaviruses with different g[p] types obtained in nizhni novgorod have been first ascertained. they belong to their affiliation to three nsp4 (a, b, and c) of the 5 known genotypes. eleven strains had nsp4 genotype. phylogenetic analysis based on the complete nucleotide sequences of the nsp4 gene revealed the sequence homology of g1p[8] viruses with the strains circulating in scandinavia and that of the g3 type v ... | 2008 | 19172905 |
| sequence analysis of unusual p[7]g5 bovine rotavirus strains reveals evidence of interspecies transmission. | by sequence and phylogenetic analyses, the 11 genomic segments of two bovine rotaviruses isolated from clinically infected calves were proven to be derived from the swine-like p[7]g5 genotype. this finding reinforced the hypothesis that interspecies transmission of completely heterologous strains can occur in nature. | 2009 | 19710284 |
| detection and analysis of bovine rotavirus strains circulating in australian calves during 2004 and 2005. | bovine rotavirus (brv) has been detected in both dairy and beef cattle herds worldwide. stool samples collected from calves in the gippsland region of victoria, australia were screened to determine the presence of brv. a total of 100 faecal samples were collected from calves with and without diarrhoea across three farms during 2004 and 2005. group a brv was detected in 26% of faecal samples (22 from diarrheic calves and four from asymptomatic calves). genotyping analysis of rotavirus positive sa ... | 2010 | 19713055 |
| development of a rotavirus vaccine: clinical safety, immunogenicity, and efficacy of the pentavalent rotavirus vaccine, rotateq. | initial approaches for rotavirus vaccines were based on the classical "jennerian" approach and utilized simian and bovine rotavirus strains, which provided cross-protection against human rotavirus strains but did not cause illness in infants and young children because of their species-specific tropism. the demonstrated efficacy of these vaccines was not consistent across studies. thus, human-animal reassortants containing an animal rotavirus backbone with human rotavirus surface g and/or p prote ... | 2009 | 20006144 |
| molecular characterization of g and p-types bovine rotavirus strains from goiás, brazil: high frequency of mixed p-type infections. | in this study, 331 samples from calves less than one month old from a dairy herd in the district of piracanjuba, state of goiás, brazil were tested for rotavirus. thirty-three samples (9.9%) tested positive for rotavirus. out of those, 31 were submitted to g and p characterization by reverse transcription followed by semi-nested polymerase chain reaction. two samples were characterized as g6p[1], three as g10p[11] and five as g6p[11]. the majority of the samples (51.6%) displayed multiple p geno ... | 2010 | 21225202 |
| phylogenetic analysis of rotavirus a nsp2 gene sequences and evidence of intragenic recombination. | the rotavirus non-structural protein nsp2 is one of the earliest and most abundant viral proteins produced during infection. this protein has multiple essential roles in the replication cycle involving rna binding, viroplasm formation, helicase and can hydrolyse the +¦-phosphate of rna and ntps acting as an rtpase and ntpase. in studying sequences from rotavirus strains isolated in australia between 1984 and 2009, the nsp2 gene was seen to be highly conserved and clustered with defined nsp2 geno ... | 2011 | 21689784 |
| diversity and zoonotic potential of rotaviruses in swine and cattle across europe. | group a rotaviruses can infect both humans and animals. individual rotavirus strains can occasionally cross species barriers and might hereby contribute to the emergence of new genotypes in heterologous hosts. the incidence and impact of zoonotic rotavirus are not well defined, and one reason for this is a lack of data about strains circulating in suspected reservoir animal hosts. in this study we report the incidence, genetic diversity, and molecular epidemiology of rotaviruses detected in dome ... | 2011 | 22079216 |
| bovine rotavirus strains circulating in beef and dairy herds in argentina from 2004 to 2010. | bovine group a rotavirus (rva) is one of the main causes of neonatal calf diarrhea worldwide. the present study reports the genotyping of bovine rva strains circulating in argentinean cattle from 2004 to 2010. additionally, a new set of typing primers was designed and tested to differentiate between g8 and g6 (lineage iii and iv) rva strains. bovine rva was detected in 30% (435/1462) of the tested samples, corresponding to 49% (207/423) of the studied outbreaks with a similar detection rates in ... | 2011 | 22503600 |
| whole genome characterization of a g6p[5] rotavirus a strain isolated from a stray cat in japan. | the whole genome of an unusual g6p[5] rotavirus a strain named frv537, which was isolated from a stray cat in japan, was characterized to determine its species of origin. the genotype constellation of frv537 was g6-p[5]-i2-r2-c2-m2-a13-n2- t6-e2-h3. no known feline rotavirus has this genotype constellation; the japanese equine strain oh-4 is the only known strain that does. while frv537 shares the same genotype with some feline rotaviruses in all genes except those encoding vp4 and nsp1, none of ... | 2016 | 27139026 |
| whole genomic analysis of an unusual human g6p[14] rotavirus strain isolated from a child with diarrhea in thailand: evidence for bovine-to-human interspecies transmission and reassortment events. | an unusual rotavirus strain, skt-27, with the g6p[14] genotypes (rva/human-wt/tha/skt-27/2012/g6p[14]), was identified in a stool specimen from a hospitalized child aged eight months with severe diarrhea. in this study, we sequenced and characterized the complete genome of strain skt-27. on whole genomic analysis, strain skt-27 was found to have a unique genotype constellation: g6-p[14]-i2-r2-c2-m2-a3-n2-t6-e2-h3. the non-g/p genotype constellation of this strain (i2-r2-c2-m2-a3-n2-t6-e2-h3) is ... | 2015 | 26421718 |
| whole-genome analysis of a rare human korean g3p rotavirus strain suggests a complex evolutionary origin potentially involving reassortment events between feline and bovine rotaviruses. | a rare human rotavirus, g3p[9] strain rva/human-tc/kor/cau12-2-51/2013/g3p[9], was isolated from the stool of a 9-year-old female hospitalized with acute watery diarrhea in august 2012 in south korea using a cell culture system, and its genome was analyzed. the complete genomic constellation of the cau12-2-51 strain revealed a novel genotype constellation for human rotavirus, g3-p[9]-i2-r2-c2-m2-a3-n2-t3-e3-h3. phylogenetic analysis revealed that the cau12-2-51 strain originated from feline- and ... | 2014 | 24818762 |
| human and bovine rotavirus strain antigens for evaluation of immunogenicity in a randomized, double-blind, placebo-controlled trial of a single dose live attenuated tetravalent, bovine-human-reassortant, oral rotavirus vaccine in indian adults. | a single dose of live attenuated tetravalent (g1-g4) bovine human reassortant rotavirus vaccine (brv-tv) was administered to healthy indian adult volunteers, who were assessed for safety and immunogenicity of the vaccine with 3:1 randomization to vaccine or placebo. all 20 adult male volunteers in the study had rotavirus specific serum iga at baseline. there were no side effects or adverse events reported. administration of brv-tv was not associated with fever, diarrhea, or altered liver transam ... | 2014 | 24704505 |
| different virulence of porcine and porcine-like bovine rotavirus strains with genetically nearly identical genomes in piglets and calves. | direct interspecies transmissions of group a rotaviruses (rva) have been reported under natural conditions. however, the pathogenicity of rva has never been directly compared in homologous and heterologous hosts. the bovine rva/cow-tc/kor/k5/2004/g5p[7] strain, which was shown to possess a typical porcine-like genotype constellation similar to that of the g5p[7] prototype rva/pig-tc/usa/osu/1977/g5p9[7] strain, was examined for its pathogenicity and compared with the porcine g5p[7] rva/pig-tc/ko ... | 2013 | 24083947 |
| whole genome sequence analysis of bovine g6p[11] rotavirus strain found in a child with gastroenteritis. | during the rotavirus strain surveillance in slovenia, g6p[11] bovine rotavirus strain was detected in a 5 months old boy with gastroenteritis. the strain was enrolled in a whole genome sequence analysis to determine its genome segment composition and genetic characteristics. genotype composition for the whole genome was g6-p[11]-i2-r2-c2-m2-a13-n2-t6-e2-h3, reflecting similarities with bovine rotavirus strains. the bovine origin of the strain was confirmed in all genome segments, showing the hig ... | 2013 | 22995281 |
| whole genome sequence analyses of three african bovine rotaviruses reveal that they emerged through multiple reassortment events between rotaviruses from different mammalian species. | animal-to-human interspecies transmission is one of the evolutionary mechanisms driving rotavirus strain diversity in humans. although quite a few studies emanating from africa revealed evidence of bovine-to-human rotavirus interspecies transmission, whole genome data of african bovine rotavirus strains are not yet available. to gain insight into the complete genome constellation of african bovine rotaviruses, the full genomes of three bovine rotavirus strains were extracted from stool samples c ... | 2012 | 22541163 |
| characterisation of a rare, reassortant human g10p[14] rotavirus strain detected in honduras. | although first detected in animals, the rare rotavirus strain g10p[14] has been sporadically detected in humans in slovenia, thailand, united kingdom and australia among other countries. earlier studies suggest that the strains found in humans resulted from interspecies transmission and reassortment between human and bovine rotavirus strains. | 2018 | 29211103 |