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isolation and characterization of a new species of kobuvirus associated with cattle.a cytopathic agent was isolated using vero cells from the culture medium of hela cells that had been used for more than 30 years in our laboratory. this agent, termed u-1 strain, was serially passed in vero cells with distinct cpe. particles of u-1 strain negatively stained with phosphotungstic acid exhibited a distinct surface that resembled aichi virus. the rna genome of u-1 strain comprises 8374 nt, with a genome organization analogous to that of picornaviruses. possible cleavage sites of the ...200314573811
bovine kobuvirus in europe. 200919402981
complete nucleotide and amino acid sequences and genetic organization of porcine kobuvirus, a member of a new species in the genus kobuvirus, family picornaviridae.kobuvirus is a new genus in the family picornaviridae. two species are currently known: aichi virus (human kobuvirus) and bovine kobuvirus (u-1). in this study, the complete nucleotide and amino acid sequences and genetic organization of porcine kobuvirus (kobuvirus/swine/s-1-hun/2007/hungary, eu787450) were determined. the structure of the s-1-hun genome, vpg-5'utr-leader protein-structural proteins (vp0, vp3, vp1)-non-structural proteins (2a-2c, 3a-3d)-3'utr-poly(a) tail, was found to be typic ...200919096904
molecular detection of bovine coronavirus in a diarrhea outbreak in pasture-feeding nellore steers in southern brazil.worldwide diarrhea outbreaks in cattle herds are more frequently detected in calves being that diarrhea outbreaks in adult cattle are not common. winter dysentery (wd) is a bovine coronavirus (bcov) enteric infection that is more reported in northern hemisphere. seasonal outbreaks of wd in adult cattle occur mainly in dairy cows. wd has not been described in beef cattle herds of tropical countries. this study describes the molecular detection of bcov in a diarrhea outbreak in beef cattle steers ...201626712361
molecular characterization and genetic diversity of bovine kobuvirus, brazil.it is suggested that bovine kobuvirus (bkv) is involved in the etiology of gastroenteric diseases especially among calves; however, this association remains unknown. this study evaluated 216 fecal samples from cattle with and without diarrhea symptoms obtained from different regions of brazil. a 216 bp fragment of the bkv 3d gene was amplified by rt-pcr in 14.4 % (31/216) of the studied samples, and 17 samples were subjected to nucleotide sequencing. all positive samples were obtained from anima ...201727623839
identification by next-generation sequencing of aichivirus b in a calf with enterocolitis and neurologic signs.an 11-d-old holstein bull calf was presented to the veterinary medical teaching hospital at the university of wisconsin-madison because of a 4-d history of diarrhea and persistent low-grade fever. initial diagnosis was enteritis caused by cryptosporidium and rotavirus. during hospitalization, the calf became stuporous and was only responsive to noxious stimuli, with hypotonia of all 4 limbs, tail, head, and neck. a cerebrospinal fluid analysis revealed xanthochromia, with marked lymphocytic pleo ...201728176615
molecular characterization and sequence analysis of the 2b region of aichivirus c strains in japan and thailand.aichivirus c is the third species in the genus kobuvirus, family picornaviridae, and the virus is circulating in pigs worldwide. aichivirus a in humans and aichivirus b in cows have been shown to associate with diarrheal diseases, however, the pathogenesis of aichivirus c has not been demonstrated clearly. in this study, the full genome nucleotide sequence of the thai strain, cmp06/2007/tha collected from stool sample of a diarrheal piglet was analyzed and identified as a variant type with a 90- ...201424837671
identification and complete genome analysis of kobuvirus in faecal samples of european roller (coracias garrulus): for the first time in a bird.the genus kobuvirus (picornaviridae) consists of three species, aichivirus a (e.g., aichi virus, which infects humans), aichivirus b and aichivirus c. kobuvirus have not been detected in non-mammal species including birds. in this study, a novel kobuvirus was identified in 3 (17 %) out of 18 faecal samples collected from european rollers (coracias garrulus) in hungary. the complete genome sequence of strain szal6-kov/2011/hun (kj934637), which was determined using a novel 5'/3' race method (dsrn ...201525195063
epidemiology of human and animal kobuviruses.kobuviruses are member of the family picornaviridae. initially, members in kobuvirus genus were named according to the basis of their host species. the viruses found in humans called "aichi virus", the viruses from cattle called "bovine kobuvirus", and the viruses isolated from pigs called "porcine kobuvirus". currently, taxonomy of kobuviruses has been proposed and the virus species have been renamed. the "aichi virus" has been renamed as "aichivirus a", "bovine kobuvirus" has been renamed as " ...201425674585
detection and characterization of aichi virus 1 in pediatric patients with diarrhea in thailand.kobuvirus is a newly discovered virus that belongs to the kobuvirus genus in picornaviridae family, which comprised of three species including aichivirus a, aichivirus b, and aichivirus c. the kobuvirus isolated from human has been classified as aichi virus 1 and belongs to aichivirus a species. the present study aimed to assess the epidemiology and to perform molecular characterization of aichi virus 1 in children admitted to hospitals with acute gastroenteritis in chiang mai, thailand. a total ...201727420653
phylogenetic analysis of kobuviruses and astroviruses from korean wild boars: 2016-2018.between 2016 and 2018, the prevalence of porcine kobuvirus (pkov) and porcine astrovirus (pastv) in korean wild boars (n = 845) was 28.0% and 10.7%, respectively. coinfection by both viruses was detected in 5.1% of boars. phylogenetic analysis revealed that 134 pkov isolates belonged to diverse lineages within the species aichivirus c; however, one strain (wkov16cn-8627) clustered with bovine kobuvirus (aichivirus b). forty-seven pastvs belonged to lineage pastv4, and only one strain (wast17jn-1 ...202134244861
molecular survey on kobuviruses in domestic and wild ungulates from northwestern italian alps.since the first identification in 1989 in humans, kobuviruses (kovs) have been identified from a wide range of animal species including carnivores, rodents, birds, ungulates, rabbits, and bats. several studies have described the identification of genetically related kovs in the fecal virome of domestic and wild animals suggesting a mutual exchange of viruses. by screening a total of 231 fecal samples from wild and domestic ungulates, kovs rna was detected in wild boars (3.2%; 2/63), chamois (4.6 ...202134195249
molecular detection and genetic characterization of kobuviruses in fecal samples collected from diarrheic cattle in korea.kobuviruses are small, non-enveloped viruses with single-stranded, positive-sense genomic rna, belonging to the family picornaviridae, a highly diverse family of important pathogens of humans and animals. in spite of discovery of bovine kobuvirus was first reported in 2003, which is several years ago, information about the virus is lacking. in this study, we first identified bovine kobuviruses from korean diarrheic cattle by rt-pcr using the generic kobuvirus primers with subsequent cloning and ...201121382521
sequence analysis of porcine kobuvirus vp1 region detected in pigs in japan and thailand.porcine kobuvirus is a new candidate species of the genus kobuvirus in the family picornaviridae, and information is still limited. the identification of porcine kobuvirus has been performed by the sequence analyses of the 3d region of the viruses. therefore, the purpose of this study was to characterize the molecular properties of vp1 nucleotide sequences of the porcine kobuviruses isolated from porcine stool samples in japan during 2009 and thailand between 2006 and 2008. in addition, previous ...201122109708
three clusters of bovine kobuvirus isolated in korea, 2008-2010.fecal samples (n = 107) were collected from cattle with ascertained or suspected diarrheal disease on korean farms during 2008-2010. of these, 37 samples tested positive for bovine kobuvirus. the 37 positive samples came from 32 cattle that exhibited diarrhea and five cattle that were non-diarrhetic. the majority of the virus-positive feces samples were from calves under 1 month of age (n = 25). nine of the 37 cattle infected with bovine kobuvirus were confirmed to have a co-infection with other ...201121399921
molecular detection of kobuvirus sequences in stool samples collected from healthy pigs in japan.this study reports the detection and genetic characterization of porcine kobuvirus, a new member of the genus kobuvirus in the family picornaviridae. among 293 fecal specimens collected from healthy pigs in 2009, in japan, 133 (45.4%) were positive for kobuvirus using rt-pcr screening method. of the positive specimens, 124 were obtained from pigs < or =6 months old, while 9 samples were from pigs >6 months old. fifty-two representative strains of kobuviruses detected in this study were randomly ...201020547246
identification and characterization of porcine kobuvirus variant isolated from suckling piglet in gansu province, china.kobuviruses comprise three species, the aichivirus a, aichivirus b, and aichivirus c (porcine kobuvirus). porcine kobuvirus is endemic to pig farms and is not restricted geographically but, rather, is distributed worldwide. the complete genomic sequences of four porcine kobuvirus strains isolated during a diarrhea outbreak in piglets in the gansu province of china were determined. two of these strains exhibited variations relative to the traditional strains. the potential 3c/3d cleavage sites of ...201324145960
the 3a protein from multiple picornaviruses utilizes the golgi adaptor protein acbd3 to recruit pi4kiiiβthe activity of phosphatidylinositol 4-kinase class iii beta (pi4kiiiβ) has been shown to be required for the replication of multiple picornaviruses, however it is unclear whether a physical association between pi4kiiiβ and the viral replication machinery exists and if it does, whether association is necessary. we examined the ability of the 3a protein from 18 different picornaviruses to form a complex with pi4kiiiβ by affinity purification of strep-tagged transiently transfected constructs foll ...201222258260
kobuviruses - a comprehensive review.kobuviruses are members of the large and growing family picornaviridae. until now, two official, aichi virus and bovine kobuvirus, and one candidate kobuvirus species, 'porcine kobuvirus', have been identified in human, cattle and swine, respectively. in addition, kobu-like viruses were detected very recently in the bat. aichi virus could be one of the causative agents of gastroenteritis in humans, and kobuviruses probably also cause diarrhoea in cattle and swine. although aichi virus has been d ...201121294214
kobuvirus (aichivirus b) infection in brazilian cattle herds.there are few studies involving the detection of aichivirus b in cattle herds worldwide, and this virus has never been diagnosed in south america. this study evaluated 222 diarrhoeic faecal samples from four brazilian geographical regions (south, southeast, midwest, and north), collected between february 2010 to may 2012. to evaluate the frequency of occurrence in different types of livestock, samples from beef (n = 105) and dairy (n = 117) cattle herds were evaluated. to determine the category ...201424590582
a distinct class of internal ribosomal entry site in members of the kobuvirus and proposed salivirus and paraturdivirus genera of the picornaviridae.the 5'-untranslated regions (5' utrs) of picornavirus genomes contain an internal ribosomal entry site (ires) that promotes the end-independent initiation of translation. picornavirus iress are classified into four structurally distinct groups, each with different initiation factor requirements. here, we identify a fifth ires class in members of kobuvirus, salivirus, and paraturdivirus genera of picornaviridae: aichi virus (av), bovine kobuvirus (bkv), canine kobuvirus (ckov), mouse kobuvirus (m ...201122114340
molecular detection of kobuviruses and recombinant noroviruses in cattle in continental europe.two genotypes (jena and newbury2) and two intergenotype recombinant strains have been recognized in bovine noroviruses. several studies have shown an apparent predominance of bovine infection with newbury2-related (genotype 2) strains. bovine stool samples were screened with two primer pairs targeting both the polymerase and the capsid genes. among the predominant genotype 2 sequences, two were genetically related to the recombinant strain thirsk10. the detection of sequences genetically related ...200919816652
prevalence and genetic diversity of bovine kobuvirus in china.a total of 166 faecal specimens from diarrheic cattle were collected in china for detection of bovine kobuvirus (bkv) by reverse transcription pcr (rt-pcr) targeting the region a portion of the 3d nonstructural protein, with an amplicon size of 631 bp. the rna corresponding to the bkv 3d region was detected in 34.9 % of faecal samples (58/166) in four major dairy-cattle-production areas in china, and sequence analysis based on the partial 3d sequences (35/58) indicated that the chinese bkvs shar ...201424366549
kobuvirus in south korean black goats.kobuviruses have been detected in humans and several animal species, including cattle, swine, sheep, canines, mice, and probably bats. while investigating the possibility of kobuviruses infecting additional animal host species, we detected kobuvirus in three fecal samples from domestic korean black goats. in a maximum parsimony tree and a bayesian tree, the 08kg680 strain fell within the bovine kobuvirus lineage, but the 09kg172 and 10kg056 strains did not fall within any of the known animal kob ...201222528642
phylogenetic analysis of vp1 and rdrp genes of brazilian aichivirus b strains involved in a diarrhea outbreak in dairy calves.aichivirus b has been reported worldwide in calves and adult cattle with and without diarrhea. the aim of this study was to describe the molecular characteristics of the rdrp and vp1 genes of aichivirus b strains identified as the most frequent etiologic agent in a neonatal diarrhea outbreak in a high-production brazilian dairy cattle herd. preliminary laboratory analysis ruled out important enteropathogens (cryptosporidium spp; eimeria spp., e. coli f5, and bovine coronavirus). fecal samples fr ...201728849283
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