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sinorhizobium americanus sp. nov., a new sinorhizobium species nodulating native acacia spp. in mexico.the sinorhizobia isolated from root nodules of acacia species native of mexico constitute a diverse group of bacteria on the basis of their metabolic enzyme electromorphs but share restriction patterns of the pcr products of 16s rrna genes and a common 500 kb symbiotic plasmid. they are distinguished from other sinorhizobium species by their levels of dna-dna hybridization and the sequence of 16s rrna and nifh genes. nolr gene hybridization patterns were found useful to identify sinorhizobia and ...200312747410
rhizobia with different symbiotic efficiencies nodulate acaciella angustissima in mexico, including sinorhizobium chiapanecum sp. nov. which has common symbiotic genes with sinorhizobium mexicanum.bacteria from nodules of the legume acaciella angustissima native to the south of mexico were characterized genetically and their nodulation and competitiveness were evaluated. phylogenetic studies derived from rpob gene sequences indicated that a. angustissima is nodulated by sinorhizobium mexicanum, rhizobium tropici, mesorhizobium plurifarium and agrobacterium tumefaciens and by bacteria related to sinorhizobium americanum, sinorhizobium terangae, rhizobium etli and rhizobium gallicum. a new ...200919120461
microsymbionts of phaseolus vulgaris in acid and alkaline soils of mexico.in order to investigate bean-nodulating rhizobia in different types of soil, 41 nodule isolates from acid and alkaline soils in mexico were characterized. based upon the phylogenetic studies of 16s rrna, atpd, glnii, reca, rpob, gyrb, nifh and nodc genes, the isolates originating from acid soils were identified as the phaseoli symbiovar of the rhizobium leguminosarum-like group and rhizobium grahamii, whereas the isolates from alkaline soils were defined as ensifer americanum sv. mediterranense ...201425294010
sinorhizobium americanum symbiovar mediterranense is a predominant symbiont that nodulates and fixes nitrogen with common bean (phaseolus vulgaris l.) in a northern tunisian field.a total of 40 symbiotic bacterial strains isolated from root nodules of common bean grown in a soil located in the north of tunisia were characterized by pcr-rflp of the 16s rrna genes. six different ribotypes were revealed. nine representative isolates were submitted to phylogenetic analyses of rrs, reca, atpd, dnak, nifh and noda genes. the strains 23c40 and 23c95 representing the most abundant ribotype were closely related to sinorhizobium americanum cfnei 156(t). s. americanum was isolated f ...201222633818
proposal of ensifer psoraleae sp. nov., ensifer sesbaniae sp. nov., ensifer morelense comb. nov. and ensifer americanum comb. nov.in a survey of rhizobia associated with the native legumes in yunnan province, china, seven and nine strains isolated from the root nodules of psoralea corylifolia, sesbania cannabina and medicago lupulina were respectively classified into the novel genomic species groups i and ii in the genus ensifer (former sinorhizobium) based on the sequence analyses of the 16s rrna gene. analyses of concatenated housekeeping genes (atpd, reca and glnii) further revealed that they were distinct lineages in t ...201323759600
calcium precipitate induced aerobic granulation.aerobic granulation is a novel biotechnology for wastewater treatment. this study refined existing aerobic granulation mechanisms as a sequencing process including formation of calcium precipitate under alkaline ph to form inorganic cores, followed by bacterial attachment and growth on these cores to form the exopolysaccharide matrix. mature granules comprised an inner core and a matrix layer and a rim layer with enriched microbial strains. the inorganic core was a mix of different crystals of c ...201525460981
genomic studies of nitrogen-fixing rhizobial strains from phaseolus vulgaris seeds and nodules.rhizobia are soil bacteria that establish symbiotic relationships with legumes and fix nitrogen in root nodules. we recently reported that several nitrogen-fixing rhizobial strains, belonging to rhizobium phaseoli, r. trifolii, r. grahamii and sinorhizobium americanum, were able to colonize phaseolus vulgaris (common bean) seeds. to gain further insight into the traits that support this ability, we analyzed the genomic sequences and proteomes of r. phaseoli (ccgm1) and s. americanum (ccgm7) stra ...201627601031
nitrogen-fixing rhizobial strains isolated from common bean seeds: phylogeny, physiology, and genome analysis.rhizobial bacteria are commonly found in soil but also establish symbiotic relationships with legumes, inhabiting the root nodules, where they fix nitrogen. endophytic rhizobia have also been reported in the roots and stems of legumes and other plants. we isolated several rhizobial strains from the nodules of noninoculated bean plants and looked for their provenance in the interiors of the seeds. nine isolates were obtained, covering most known bean symbiont species, which belong to the rhizobiu ...201425002426
[phenotypic and genotypic characterization of twelve rhizobial isolates from different regions of venezuela].rhizobial taxonomy and systematics have progressed substantially, nevertheless, few studies have been developed on venezuelan species. this study evaluated the phenotypic and genetic variation between 12 venezuelan indigenous rhizobial isolates and 10 international referential strains, by phenotypical traits and dna molecular markers. in this regard, a pcr-rflp of the 16s rdna gene, the presence of large plasmids, metabolic assays in solid media, salinity resistance, ph and temperature growth co ...201122017109
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