| construction of an infectious cdna clone of aichi virus (a new member of the family picornaviridae) and mutational analysis of a stem-loop structure at the 5' end of the genome. | aichi virus is the type species of a new genus, kobuvirus, of the family picornaviridae. in this study, we constructed a full-length cdna clone of aichi virus whose in vitro transcripts were infectious to vero cells. during construction of the infectious cdna clone, a novel sequence of 32 nucleotides was identified at the 5' end of the genome. computer-assisted prediction of the secondary structure of the 5' end of the genome, including the novel sequence, suggested the formation of a stable ste ... | 2001 | 11483747 |
| phylogenetic analysis of ljungan virus and a-2 plaque virus, new members of the picornaviridae. | in addition to the viruses belonging to the nine proposed genera of the picornaviridae, enterovirus, rhinovirus, cardiovirus, aphtovirus, hepatovirus, parechovirus, kobuvirus, erbovirus and teschovirus, two new members of this family have recently been discovered. three strains of ljungan virus (lv) were isolated from bank voles (clethrionomys glareolus) and a-2 plaque virus (a-2) was isolated from human sera. to study the genetic relationship between these recently discovered viruses and the me ... | 2002 | 11955639 |
| genomic evidence that simian virus 2 and six other simian picornaviruses represent a new genus in picornaviridae. | analysis of the vp1 capsid protein coding region of simian virus (sv) 2, sv16, sv18, sv42, sv44, sv45, and sv49 demonstrates that they are clearly distinct from members of the enterovirus genus and from members of other existing picornavirus genera. to further characterize this group of viruses and to clarify their classification within the picornaviridae, we have determined the complete genomic sequence of sv2 (8126 nucleotides). the genome was typical of members of picornaviridae, encoding a s ... | 2003 | 14517081 |
| isolation and characterization of a new species of kobuvirus associated with cattle. | a cytopathic agent was isolated using vero cells from the culture medium of hela cells that had been used for more than 30 years in our laboratory. this agent, termed u-1 strain, was serially passed in vero cells with distinct cpe. particles of u-1 strain negatively stained with phosphotungstic acid exhibited a distinct surface that resembled aichi virus. the rna genome of u-1 strain comprises 8374 nt, with a genome organization analogous to that of picornaviruses. possible cleavage sites of the ... | 2003 | 14573811 |
| phylogeny of the picornaviridae and differential evolutionary divergence of picornavirus proteins. | a rooted phylogenetic analysis of the viral family picornaviridae identified several major clusters within this viral family. the genus parechovirus, including ljungan virus, was found to be basal to the family. within the family, there were two major subgroups: (1) a cluster including viruses assigned to the genera enterovirus and rhinovirus; and (2) a cluster including teschovirus, cardiovirus, erbovirus, aphthovirus, and probably kobuvirus. within the former cluster, the genera enterovirus an ... | 2004 | 15157632 |
| an rna extraction protocol for shellfish-borne viruses. | the gptt virus rna extraction method, originally developed for extraction of human norovirus and hepatitis a virus rnas from contaminated shellfish, was evaluated for extraction of rna from aichi virus strain a846/88 (aiv), coxsackievirus strains a9 (cav9) and b5 (cbv5), murine norovirus (strain mnv-1), and the norovirus surrogate, feline calicivirus (fcv) strain kcd, for the purpose of rt-pcr detection within seeded oyster (crassostrea virginica) extracts. the rt-pcr equivalent sensitivities ob ... | 2007 | 17184849 |
| isolation and molecular characterization of aichi viruses from fecal specimens collected in japan, bangladesh, thailand, and vietnam. | aichi virus is a new member of the family picornaviridae, genus kobuvirus, and is associated with human gastroenteritis. this study detected aichi virus in 28 of 912 fecal specimens which were negative for rotavirus, adenovirus, norovirus, sapovirus, and astrovirus and were collected in japan, bangladesh, thailand, and vietnam during 2002 to 2005. | 2007 | 17522267 |
| [analysis of aichi virus replication]. | | 2007 | 17824229 |
| [analysis of aichi virus replication]. | aichi virus is a member of the family picornaviridae. this virus was first isolated in 1989 from a stool specimen from a patient with oyster-associated gastroenteritis in aichi, japan. we analyzed the function of the 5' terminal region of the genome and the leader protein in virus replication. the results indicate that both the 5' terminal region of the genome and the leader protein are involved in viral rna replication and encapsidation. | 2007 | 18040156 |
| prevalence and genetic diversity of aichi virus strains in stool samples from community and hospitalized patients. | aichi virus has been proposed as a causative agent of gastroenteritis. a total of 457 stool specimens from children hospitalized with acute diarrhea and 566 stool specimens from adults and children involved in 110 gastroenteritis outbreaks were screened for the presence of aichi virus by reverse transcription-pcr (rt-pcr) amplification of the genomic region of the 3c and 3d (3cd) nonstructural proteins. our results show a low incidence of aichi virus in pediatric samples and the existence of mix ... | 2008 | 18256215 |
| seroprevalence distribution of aichi virus among a french population in 2006-2007. | little is known about the epidemiology of aichi virus, which is a new member of the family picornaviridae, in the genus kobuvirus. we report here on seroprevalence in france. sera were screened using an enzyme-linked immunosorbent assay (elisa) for immunoglobulin g. of 972 sera tested, seroprevalence ranged from 25% for the 7-month-to-9-year-old age group to about 85% for the 30-to-39-year-old age group and older age groups. our elisa correlated well with the microneutralization technique. this ... | 2008 | 18446423 |
| interaction between polypeptide 3abc and the 5'-terminal structural elements of the genome of aichi virus: implication for negative-strand rna synthesis. | secondary structural elements at the 5' end of picornavirus genomic rna function as cis-acting replication elements and are known to interact specifically with viral p3 proteins in several picornaviruses. in poliovirus, ribonucleoprotein complex formation at the 5' end of the genome is required for negative-strand synthesis. we have previously shown that the 5'-end 115 nucleotides of the aichi virus genome, which are predicted to fold into two stem-loops (sl-a and sl-c) and one pseudoknot (pk-b) ... | 2008 | 18448525 |
| sequence analysis of the capsid gene of aichi viruses detected from japan, bangladesh, thailand, and vietnam. | sequence analysis of the capsid gene of aichi viruses was performed on 12 strains detected in japan, bangladesh, thailand, and vietnam during 2002-2005. the phylogenetic tree constructed from 17 nucleotide sequences of the capsid gene of the strains studied and reference strains demonstrated that aichi virus strains clustered into two branches. a classification of aichi viruses based on the capsid gene was proposed, in which lineage i consists of the aichi virus strains detected from japan, thai ... | 2008 | 18461624 |
| bovine kobuviruses from cattle with diarrhea. | | 2008 | 18507924 |
| uv light inactivation of hepatitis a virus, aichi virus, and feline calicivirus on strawberries, green onions, and lettuce. | a majority of illnesses caused by foodborne viruses are associated with fresh produce. fruits and vegetables may be considered high-risk foods, as they are often consumed raw without a specific inactivation step. therefore, there is a need to evaluate nonthermal treatments for the inactivation of foodborne pathogens. this study investigates the uv inactivation of three viruses: feline calicivirus (a surrogate for norovirus), and two picornaviruses, hepatitis a virus and aichi virus. three produc ... | 2008 | 18522022 |
| aichi virus 2a protein is involved in viral rna replication. | the aichi virus 2a protein is not a protease, unlike many other picornavirus 2a proteins, and it is related to a cellular protein, h-rev107. here, we examined the replication properties of two 2a mutants in vero cells and a cell-free translation/replication system. in one mutant, amino acids 36 to 126 were replaced with an unrelated amino acid sequence. in the other mutant, the nc motif conserved in the h-rev107 family of proteins was changed to alanine residues. the two mutations abolished viru ... | 2008 | 18653460 |
| detection of human enteric viruses in japanese clams. | a total of 57 clam packages that were collected from supermarkets and fish markets from 11 different sites in western japan between 8 december 2005 and 6 september 2006 were examined for human enteric viruses (i.e., norovirus, aichi virus, rotavirus, adenovirus, hepatitis a virus, and astrovirus), using pcr and reverse transcription pcr. sixty-one percent of the packages were contaminated with one type of virus, 9% had two different types of viruses, 28% had three different types of viruses, and ... | 2008 | 18724766 |
| aichi virus, norovirus, astrovirus, enterovirus, and rotavirus involved in clinical cases from a french oyster-related gastroenteritis outbreak. | following a flooding event close to a shellfish production lagoon, 205 cases of gastroenteritis were linked to oyster consumption. twelve stool samples from different individuals were collected. analysis showed that eight samples were positive for multiple enteric viruses, and one stool sample had seven different enteric viruses. analysis of shellfish implicated in the outbreak allowed detection of the same diversity of enteric viruses, with some viral genomic sequences being identical to those ... | 2008 | 18842942 |
| comparative recovery of foodborne viruses from fresh produce. | a large percentage of foodborne outbreaks are caused by viruses, and outbreaks associated with fresh produce have increased over the past decade within the united states. virus recovery from food is of the utmost importance in determining the cause of viral outbreaks. while there are many experimental studies investigating viruses on fruits and vegetables, there is a lack of standard techniques concerning the initial inoculation and recovery of viruses. this study investigates the efficiency of ... | 2008 | 18991544 |
| candidate new species of kobuvirus in porcine hosts. | | 2008 | 19046542 |
| development of genotype-specific primers for differentiation of genotypes a and b of aichi viruses. | a nested polymerase chain reaction method using genotype-specific primers based on the capsid gene was developed to differentiate between genotypes a and b of aichi viruses. results of the study showed that the pcr using newly designed genotype-specific primers could generate appropriate pcr products from all 17 samples tested, the newly developed primers could differentiate genotype a from genotype b, and all matched those obtained by nucleotide sequencing of the capsid regions. the nested pcr ... | 2009 | 19059437 |
| complete nucleotide and amino acid sequences and genetic organization of porcine kobuvirus, a member of a new species in the genus kobuvirus, family picornaviridae. | kobuvirus is a new genus in the family picornaviridae. two species are currently known: aichi virus (human kobuvirus) and bovine kobuvirus (u-1). in this study, the complete nucleotide and amino acid sequences and genetic organization of porcine kobuvirus (kobuvirus/swine/s-1-hun/2007/hungary, eu787450) were determined. the structure of the s-1-hun genome, vpg-5'utr-leader protein-structural proteins (vp0, vp3, vp1)-non-structural proteins (2a-2c, 3a-3d)-3'utr-poly(a) tail, was found to be typic ... | 2009 | 19096904 |
| detection of multiple sapovirus genotypes and genogroups in oyster-associated outbreaks. | this report describes multiple viruses in stool specimens from oyster-associated gastroenteritis. eleven outbreaks of oyster-associated gastroenteritis were examined for enteric viruses between january 2002 and march 2006 in japan. multiple norovirus genotypes were detected in all outbreaks; moreover, kobuvirus, sapovirus, and astrovirus were also detected in 6, 3, and 1 of the 11 outbreaks, respectively. notably, multiple sapovirus genogroups were detected in the stool specimens from subjects i ... | 2009 | 19168964 |
| surveillance of pathogens in outpatients with gastroenteritis and characterization of sapovirus strains between 2002 and 2007 in kumamoto prefecture, japan. | infectious acute gastroenteritis is an important public health problem worldwide. a total of 639 stool specimens were tested for the presence of diarrhea pathogens. the specimens were from outpatients with acute gastroenteritis who consulted the pediatric clinic in kumamoto prefecture, japan, from june 2002 to december 2007. of these, 421 (65.9%) were positive for diarrhea pathogens. among them were norovirus (nov) in 260 (61.8%), sapovirus (sav) in 81 (19.2%), rotavirus in 49 (11.6%), adenoviru ... | 2009 | 19382269 |
| bovine kobuvirus in europe. | | 2009 | 19402981 |
| candidate porcine kobuvirus, china. | | 2009 | 19402982 |
| detection and genomic characterization of aichi viruses in stool samples from children in monastir, tunisia. | aichi virus has been associated with acute gastroenteritis in adults and children. stool samples were collected from 788 tunisian children suffering from diarrhea. aichi virus was found in 4.1% of the cases. the high proportion of monoinfections and the high frequency of hospitalizations support the role of aichi virus in pediatric gastroenteritis. | 2009 | 19474269 |
| the complete genome of klassevirus - a novel picornavirus in pediatric stool. | diarrhea kills 2 million children worldwide each year, yet an etiological agent is not found in approximately 30-50% of cases. picornaviral genera such as enterovirus, kobuvirus, cosavirus, parechovirus, hepatovirus, teschovirus, and cardiovirus have all been found in human and animal diarrhea. modern technologies, especially deep sequencing, allow rapid, high-throughput screening of clinical samples such as stool for new infectious agents associated with human disease. | 2009 | 19538752 |
| klassevirus 1, a previously undescribed member of the family picornaviridae, is globally widespread. | diarrhea is the third leading infectious cause of death worldwide and is estimated to be responsible for approximately 2 million deaths a year. while many infectious causes of diarrhea have been established, approximately 40% of all diarrhea cases are of unknown etiology. in an effort to identify novel viruses that may be causal agents of diarrhea, we used high throughput mass sequencing to analyze stool samples collected from patients with acute diarrhea. | 2009 | 19552824 |
| detection of aichi virus shedding in a child with enteric and extraintestinal symptoms in hungary. | aichi virus, genus kobuvirus, family picornaviridae, has been proposed as a causative agent of gastroenteritis in human. although high seroprevalence has been detected, it has been identified in only a few cases. we report detection of aichi virus in hungary. a total of 65 stool samples were tested retrospectively, collected from children with diarrhea, by reverse transcription-polymerase chain reaction. one (1.5%) sample from a 3-year-old girl was positive. besides diarrhea, fever, purulent con ... | 2009 | 19669615 |
| molecular detection of kobuviruses and recombinant noroviruses in cattle in continental europe. | two genotypes (jena and newbury2) and two intergenotype recombinant strains have been recognized in bovine noroviruses. several studies have shown an apparent predominance of bovine infection with newbury2-related (genotype 2) strains. bovine stool samples were screened with two primer pairs targeting both the polymerase and the capsid genes. among the predominant genotype 2 sequences, two were genetically related to the recombinant strain thirsk10. the detection of sequences genetically related ... | 2009 | 19816652 |
| aichi virus strains in children with gastroenteritis, china. | | 2009 | 19861087 |
| overall linkage map of the nonstructural proteins of aichi virus. | aichi virus (aiv), which is associated with acute gastroenteritis in humans, is a member of the genus kobuvirus of the family picornaviridae. picornavirus genome replication occurs in replication complexes that include viral nonstructural proteins, host proteins and viral rna. in poliovirus, all nonstructural proteins are found in the replication complexes, suggesting the ability of the viral nonstructural proteins to interact with each other. in this study, we examined the interactions between ... | 2010 | 19879907 |
| aichi virus infection in children with acute gastroenteritis in finland. | aichi virus has been proposed as a novel causative agent of acute gastroenteritis. in addition to several asian countries, south america and africa, aichi virus has also recently been found in europe. our objective was to study the causative role of aichi virus in children with acute gastroenteritis in finland. we analysed 595 stool specimens from infants in an efficacy trial of rotavirus vaccine and 468 stool specimens from children in a hospital-based epidemiological and aetiological study of ... | 2010 | 19961643 |
| porcine kobuvirus in piglets, thailand. | | 2009 | 19961712 |
| seroprevalence of aichi virus in a spanish population from 2007 to 2008. | viruses are among the most common causes of acute gastroenteritis. in recent years, new viruses causing outbreaks of acute gastroenteritis have been described. among these, aichi virus was identified in japan in 1989. aichi virus belongs to the kobuvirus genus in the family picornaviridae. this virus has been detected in outbreaks of gastroenteritis associated with oyster consumption and in pediatric stool samples, but little is known about its epidemiology or pathogenesis. in the present study, ... | 2010 | 20164249 |
| virus' (ms2, phix174, and aichi) attachment on sand measured by atomic force microscopy and their transport through sand columns. | atomic force microscopy (afm) was used to study the attachment of phix174, ms2, and aichi viruses on sands of different surface properties: oxide-removed (clean), goethite-coated, and aluminum oxide-coated. interaction forces between viruses and sand surfaces were measured by contact mode afm using tips coated with particles of each virus. column experiments were conducted to quantify the macroscopic transport and retention of the viruses in sand. the average adhesion force measured with afm was ... | 2010 | 20205469 |
| evolution of porcine kobuvirus infection, hungary. | porcine kobuvirus was first identified in early 2007 in hungary. originally thought to be confined to the intestine, almost 2 years later the virus was found in the blood of clinically healthy pigs on the same farm. porcine kobuvirus may be widely distributed on pig farms worldwide. | 2010 | 20350391 |
| kobuvirus in domestic sheep, hungary. | | 2010 | 20409388 |
| molecular detection and characterization of aichi viruses in sewage-polluted waters of venezuela. | the circulation of aichi virus in a major urban area was demonstrated using molecular detection with samples recovered from a major river polluted with sewage discharges in caracas, venezuela. five out of 11 water samples studied were positive, being classified by phylogenetic analysis as genotype b. analysis of sewage waters appears to be a useful methodology to uncover the presence of a hitherto undetected fecal pathogen in a given geographical area. | 2010 | 20418428 |
| aichi virus igg seroprevalence in tunisia parallels genomic detection and clinical presentation in children with gastroenteritis. | aichi virus has been described as a novel causative agent of gastroenteritis in humans. in this study, we report the seroprevalence distribution of aichi virus in tunisia. a panel of 1,000 sera was screened by applying an enzyme-linked immunosorbent assay for immunoglobulin g specific for aichi virus. a considerable prevalence (92%) of antibody to aichi virus was found across all age groups. the specific anti-aichi virus antibodies increased with age, from a high rate (68.8%) in children under 1 ... | 2010 | 20484569 |
| molecular detection of kobuvirus sequences in stool samples collected from healthy pigs in japan. | this study reports the detection and genetic characterization of porcine kobuvirus, a new member of the genus kobuvirus in the family picornaviridae. among 293 fecal specimens collected from healthy pigs in 2009, in japan, 133 (45.4%) were positive for kobuvirus using rt-pcr screening method. of the positive specimens, 124 were obtained from pigs < or =6 months old, while 9 samples were from pigs >6 months old. fifty-two representative strains of kobuviruses detected in this study were randomly ... | 2010 | 20547246 |
| comparative analysis of six genome sequences of three novel picornaviruses, turdiviruses 1, 2 and 3, in dead wild birds, and proposal of two novel genera, orthoturdivirus and paraturdivirus, in the family picornaviridae. | in this territory-wide molecular epidemiology study of picornaviruses, involving 6765 dead wild birds from 201 species in 50 families over a 12 month period, three novel picornaviruses, turdiviruses 1, 2 and 3 (tv1, tv2 and tv3), were identified from birds of different genera in the family turdidae. in contrast to many other viruses in birds of the family turdidae or viruses of the family picornaviridae, tv1, tv2 and tv3 were found exclusively in the autumn and winter months. two genomes each of ... | 2010 | 20554801 |
| first molecular detection of aichi virus in sewage and shellfish samples in the monastir region of tunisia. | the aims of our investigations were (1) to look for aichi virus in environmental samples and (2) to compare the aichi virus strains in both clinical and environmental samples in order to evaluate the role of environmental contamination as a possible vehicle for viral transmission. aichi virus was detected in 15 (6%) sewage samples and in 4 (6.6%) shellfish samples. aichi virus was identified for the first time in water samples. phylogenetic analysis revealed several clusters that occurred sequen ... | 2010 | 20607319 |
| fate of human enteric viruses during dairy manure-based composting. | murine norovirus 1 (mnv-1), aichi virus (aiv), and human adenovirus 41 (ad41) were seeded in dairy manure and composted for 60 days, and both the stability of virus genomes and infectious viruses were evaluated. for compost started in late fall, pile temperature reached approximately 54.5 degrees c on day 1 and remained between 55 and 60 degrees c for 3 days. for viral genomes, aiv had an approximate 1.4-log loss of viral genome after 1 day and more than a 3.1-log loss after 2 days; while for mn ... | 2010 | 20819370 |
| molecular detection of porcine kobuviruses in pigs in korea and their association with diarrhea. | kobuviruses are small, non-enveloped viruses with a single-stranded, positive-sense genomic rna, belonging to the family picornaviridae, a highly diverse family of important pathogens of human and other animals. porcine kobuvirus has been found recently, and consequently, information about the virus is lacking. in this study, we identified porcine kobuviruses from pigs in korea by rt-pcr, cloning and sequencing, and we showed the existence of genetic diversity among geographically separated porc ... | 2010 | 20820823 |
| [detection of aichi virus in stool samples from children in lanzhou]. | to identification and analysis aichi virus from diarrhea and normal children in lanzhou, and discuss the relationship between aichi virus and infant diarrhea. | 2010 | 20848838 |
| porcine kobuvirus from pig stool in korea. | to survey for porcine kobuvirus infection, a total of 119 pig fecal samples in korea were collected from three pig farms with good breeding facilities in three provinces. forty-three (36.1%) of the fecal samples tested were positive for porcine kobuvirus. in addition, porcine epidemic diarrhea virus (pedv) and transmissible gastroenteritis virus (tgev) infections were not identified in any of the fecal samples, but porcine group a rotavirus (gar) infection was identified in 11.8% (14/119) of the ... | 2010 | 21161358 |
| prevalence and genetic diversity of aichi viruses in wastewater and river water in japan. | aichi virus (aiv) genomes were detected in 12 (100%) influent and 11 (92%) effluent wastewater and 36 (60%) river water samples. among 260 strains identified, 255 were genotype a and 5 were genotype b. this is the first report describing the molecular characterization of aivs in aquatic environments in japan. | 2011 | 21257803 |
| analysis and characterization of the complete genome of a member of a new species of kobuvirus associated with swine. | a virus belonging to a new species in the genus kobuvirus, family picornaviridae, was first isolated in 2008 from apparently healthy pigs in hungary and china. we report the complete genome sequence and the genetic organization of the novel porcine kobuvirus strain y-1-chi, which was identified in china. the rna genome of strain y-1-chi contains 8210 nucleotides (nt) and has an organization similar to that of other picornaviruses. the full-length nucleotide sequence of y-1-chi was 88.62%, 58.66% ... | 2011 | 21274731 |
| kobuviruses - a comprehensive review. | kobuviruses are members of the large and growing family picornaviridae. until now, two official, aichi virus and bovine kobuvirus, and one candidate kobuvirus species, 'porcine kobuvirus', have been identified in human, cattle and swine, respectively. in addition, kobu-like viruses were detected very recently in the bat. aichi virus could be one of the causative agents of gastroenteritis in humans, and kobuviruses probably also cause diarrhoea in cattle and swine. although aichi virus has been d ... | 2011 | 21294214 |
| molecular detection and genetic characterization of kobuviruses in fecal samples collected from diarrheic cattle in korea. | kobuviruses are small, non-enveloped viruses with single-stranded, positive-sense genomic rna, belonging to the family picornaviridae, a highly diverse family of important pathogens of humans and animals. in spite of discovery of bovine kobuvirus was first reported in 2003, which is several years ago, information about the virus is lacking. in this study, we first identified bovine kobuviruses from korean diarrheic cattle by rt-pcr using the generic kobuvirus primers with subsequent cloning and ... | 2011 | 21382521 |
| three clusters of bovine kobuvirus isolated in korea, 2008-2010. | fecal samples (n = 107) were collected from cattle with ascertained or suspected diarrheal disease on korean farms during 2008-2010. of these, 37 samples tested positive for bovine kobuvirus. the 37 positive samples came from 32 cattle that exhibited diarrhea and five cattle that were non-diarrhetic. the majority of the virus-positive feces samples were from calves under 1 month of age (n = 25). nine of the 37 cattle infected with bovine kobuvirus were confirmed to have a co-infection with other ... | 2011 | 21399921 |
| aichi virus shedding in high concentrations in patients with acute diarrheac. | we assessed aichi virus shedding in patients with gastroenteritis and negative test results for other viral and bacterial infections. high concentrations of up to 1.32 × 1012 rna copies/g stool were found in 10 (2.0%) of 499 outpatients sampled in northern germany, 2004. these data substantiate aichi virus pathogenicity in humans. | 2011 | 21801647 |
| porcine kobuvirus from pig stool specimens in shanghai, china. | in this study, a total of 116 stool specimens were collected from pigs of different ages in three pig farms of shanghai in china during 2010. forty-five (38.8%) stool specimens were positive for porcine kobuvirus using reverse transcriptase-polymerase chain reaction (rt-pcr). the prevalence rate of porcine kobuvirus in the three pig farms which are located in minhang district, qingpu district and fengxian district was 46.7%(21/45), 35.1%(13/37), and 32.4%(11/34), respectively. we demonstrated th ... | 2011 | 21789634 |
| viruses in diarrhetic dogs include novel kobuviruses and sapoviruses. | the close interactions of dogs with humans and surrounding wild life provide frequent opportunities for cross-species virus transmissions. in order to initiate an unbiased characterization of the eukaryotic viruses in the gut of dogs we used deep sequencing of partially purified viral-capsid protected nucleic acids from the feces of eighteen diarrhetic dogs. we identified known canine parvoviruses, coronaviruses, and rotaviruses, and characterized the genomes of the first reported canine kobuvir ... | 2011 | 21775584 |
| first detection of kobuvirus in farm animals in brazil and the netherlands. | animal kobuviruses have been described in pigs, cattle, sheep and bats in countries in asia and europe. the virus can be detected in fecal and serum samples of infected animals with or without diarrhea, but most of the clinical as well as epidemiological features of kobuvirus infection are still unknown. this study reports the first detection of kobuvirus in farm animals from brazil and the netherlands and the molecular analysis of the detected strains. in brazil, 53% (61/115) of the pigs (suckl ... | 2011 | 21763785 |
| sequence analysis reveals mosaic genome of aichi virus. | abstract: aichi virus is a positive-sense and single-stranded rna virus, which demonstrated to be related to diarrhea of children. in the present study, phylogenetic and recombination analysis based on the aichi virus complete genomes available in genbank reveal a mosaic genome sequence [genbank: fj890523], of which the nt 261-852 region (the nt position was based on the aligned sequence file) shows close relationship with ab010145/japan with 97.9% sequence identity, while the other genomic regi ... | 2011 | 21819561 |
| the mechanism of translation initiation on aichivirus rna mediated by a novel type of picornavirus ires. | picornavirus mrnas contain iress that sustain their translation during infection, when host protein synthesis is shut off. the major classes of picornavirus iress (types 1 and 2) have distinct structures and sequences, but initiation on both is determined by their specific interaction with eif4g. we report here that aichivirus (av), a member of the kobuvirus genus of picornaviridae, contains an ires that differs structurally from type 1 and type 2 iress. its function similarly involves interacti ... | 2011 | 21873976 |
| sequence analysis of porcine kobuvirus vp1 region detected in pigs in japan and thailand. | porcine kobuvirus is a new candidate species of the genus kobuvirus in the family picornaviridae, and information is still limited. the identification of porcine kobuvirus has been performed by the sequence analyses of the 3d region of the viruses. therefore, the purpose of this study was to characterize the molecular properties of vp1 nucleotide sequences of the porcine kobuviruses isolated from porcine stool samples in japan during 2009 and thailand between 2006 and 2008. in addition, previous ... | 2011 | 22109708 |
| Characterization of a canine homolog of human Aichivirus. | Many of our fatal "civilization" infectious diseases have arisen from domesticated animals. Although picornaviruses infect most mammals, infection of a companion animal is not known. Here we describe the identification and genomic characterization of the first canine picornavirus. Canine kobuvirus (CKoV), identified in stool samples from dogs with diarrhea, has a genomic organization typical of a picornavirus and encodes a 2,469-amino-acid polyprotein flanked by 5' and 3' untranslated regions. C ... | 2011 | 21880761 |
| a distinct class of internal ribosomal entry site in members of the kobuvirus and proposed salivirus and paraturdivirus genera of the picornaviridae. | the 5'-untranslated regions (5' utrs) of picornavirus genomes contain an internal ribosomal entry site (ires) that promotes the end-independent initiation of translation. picornavirus iress are classified into four structurally distinct groups, each with different initiation factor requirements. here, we identify a fifth ires class in members of kobuvirus, salivirus, and paraturdivirus genera of picornaviridae: aichi virus (av), bovine kobuvirus (bkv), canine kobuvirus (ckov), mouse kobuvirus (m ... | 2011 | 22114340 |
| molecular characterization of a porcine kobuvirus strain in china. | porcine kobuvirus was first identified in 2007 in hungary. the virus has been detected in several asian countries. in our study, the complete genome of the recently identified porcine kobuvirus strain sh-w-chn was amplified by rt-pcr and was sequenced. dendrograms indicated that sh-w-chn is closely related to other porcine kobuviruses. to identify sites of possible recombination within the genome of the sh-w-chn strain, the simplot program was used to perform recombination analysis. the results ... | 2011 | 22209786 |
| emerging and re-emerging swine viruses. | in the past two decades or so, a number of viruses have emerged in the global swine population. some, such as porcine reproductive and respiratory syndrome virus (prrsv) and porcine circovirus type 2 (pcv2), cause economically important diseases in pigs, whereas others such as porcine torque teno virus (ttv), now known as torque teno sus virus (ttsuv), porcine bocavirus (pbov) and related novel parvoviruses, porcine kobuvirus, porcine toroviruses (ptov) and porcine lymphotropic herpesviruses (pl ... | 2012 | 22225855 |
| the 3a protein from multiple picornaviruses utilizes the golgi adaptor protein acbd3 to recruit pi4kiiiβ | the activity of phosphatidylinositol 4-kinase class iii beta (pi4kiiiβ) has been shown to be required for the replication of multiple picornaviruses, however it is unclear whether a physical association between pi4kiiiβ and the viral replication machinery exists and if it does, whether association is necessary. we examined the ability of the 3a protein from 18 different picornaviruses to form a complex with pi4kiiiβ by affinity purification of strep-tagged transiently transfected constructs foll ... | 2012 | 22258260 |