| mutational evidence that the vpg is involved in the replication and not the movement of pea enation mosaic virus-1. | pea enation mosaic disease is caused by an obligatory association between the enamovirus pea enation mosaic virus-1 (pemv-1) and the umbravirus pea enation mosaic virus-2 (pemv-2). encapsidated rnas 1 and 2 are covalently linked to a 3138 da vpg encoded by the rna of pemv-1. to determine the role of the vpg in the pathogenicity of pemv (pemv-1+pemv-2), the infectivity of clones with mutations in key amino acids in the vpg was evaluated in protoplasts and in plants. using quantitative, real-time ... | 2000 | 10725438 |
| mechanical transmission of potato leafroll virus. | like typical luteoviruses, potato leafroll virus (plrv) cannot be transmitted mechanically by rubbing plants with solutions containing virus particles. however, plrv was found to be mechanically transmissible from extracts of plants that had been inoculated by viruliferous aphids and then post-inoculated with pea enation mosaic virus-2 (pemv-2). unlike the asymptomatic infections induced by either virus alone, double infections in nicotiana benthamiana induced necrotic symptoms with some line pa ... | 2000 | 11038393 |
| umbravirus gene expression helps potato leafroll virus to invade mesophyll tissues and to be transmitted mechanically between plants. | potato leafroll virus (plrv) was mechanically transmissible when inocula also contained the umbravirus pea enation mosaic virus-2 (pemv-2). in plants infected with plrv and pemv-2, plrv accumulated in clusters of mesophyll cells in both inoculated and systemically infected leaves. no transmissions were obtained by coinoculation with potato virus y, potato virus x (pvx), tobacco mosaic virus, or cucumber mosaic virus (cmv), although plrv was transmissible from mixtures with cmv(orf4) (a recombina ... | 2001 | 11485404 |
| umbravirus-encoded proteins both stabilize heterologous viral rna and mediate its systemic movement in some plant species. | the proteins encoded by open reading frame 3 (orf3) of the umbraviruses pea enation mosaic virus-2 and tobacco mottle virus, like that of groundnut rosette virus, mediated the movement of viral rna through the phloem of infected nicotiana benthamiana or n. clevelandii plants when they were expressed from chimeric tobacco mosaic virus in place of the coat protein. however, these chimeras did not move systemically in n. tabacum. in lysates of n. benthamiana or n. tabacum protoplasts, the chimeric ... | 2001 | 11601910 |
| analysis of the accumulation of pea enation mosaic virus genomes in seed tissues and lack of evidence for seed transmission in pea (pisum sativum). | pea enation mosaic virus (pemv) is an important virus disease of pea. international movement of commercial pea cultivars and germplasm can be problematic due to uncertainty about seed transmission of the viruses responsible for the disease. whether pemv is seedborne was assessed by collecting developing seed from infected plants and determining the relative concentrations of the pemv-1 and pemv-2 viral genomes using quantitative real-time reverse-transcription polymerase chain reaction. the rela ... | 2009 | 19821732 |
| genomic characterization of pea enation mosaic virus-2 from the pacific northwestern usa. | | 2011 | 21837418 |
| synergistic infection of bryv and pemv 2 increases the accumulations of both bryv and bryv-derived sirnas in nicotiana benthamiana. | viral synergism is caused by co-infection of two unrelated viruses, leading to more severe symptoms or increased titres of one or both viruses. synergistic infection of phloem-restricted poleroviruses and umbraviruses has destructive effects on crop plants. the mechanism underlying this synergy remains elusive. in our study, synergism was observed in co-infections of a polerovirus brassica yellows virus (bryv) and an umbravirus pea enation mosaic virus 2 (pemv 2) on nicotiana benthamiana, which ... | 2017 | 28345652 |
| quantification of pea enation mosaic virus 1 and 2 during infection of pisum sativum by one step real-time rt-pcr. | pea enation mosaic virus 1 (pemv1) and pea enation mosaic virus 2 (pemv2) are two viruses in an obligate symbiosis that cause pea enation mosaic disease mainly in plants in the fabaceae family. this virus system is a valuable model to investigate plant virus replication, movement and vector transmission. thus, here we describe growth conditions, virus detection methods, and virus accumulation behavior. to measure the accumulation and movement of pemv1 and pemv2 in plants during the course of inf ... | 2017 | 27915037 |
| role of pea enation mosaic virus coat protein in the host plant and aphid vector. | understanding the molecular mechanisms involved in plant virus-vector interactions is essential for the development of effective control measures for aphid-vectored epidemic plant diseases. the coat proteins (cp) are the main component of the viral capsids, and they are implicated in practically every stage of the viral infection cycle. pea enation mosaic virus 1 (pemv1, enamovirus, luteoviridae) and pea enation mosaic virus 2 (pemv2, umbravirus, tombusviridae) are two rna viruses in an obligate ... | 2016 | 27869713 |
| differential use of 3'cites by the subgenomic rna of pea enation mosaic virus 2. | the genomic rna (grna) of pea enation mosaic virus 2 (pemv2) is the template for p33 and -1 frameshift product p94. the pemv2 subgenomic rna (sgrna) encodes two overlapping orfs, p26 and p27, which are required for movement and stability of the grna. efficient translation of p33 requires two of three 3' proximal cap-independent translation enhancers (3'cites): the kl-tss, which binds ribosomes and engages in a long-distance interaction with the 5'end; and the adjacent eif4e-binding pte. unlike t ... | 2017 | 28750323 |